BACTERIOLOGY 231 PLATE MEDIA MEDIA CARBOHYDRATE INHIBITOR pH INDICATOR RESULT Alpha hemolysis Incomplete or partial lysis with greening Beta hemolysis Complete lysis with colorless zone [diffusion of hemoglobin into the surrounding medium] BAP Blood agar Outdated human blood or 5% defribinated sheep blood (preferred) Trypticase Soy Agar or Nutrient agar {Nonselective Differential agar} Gamma hemolysis Nonhemolytic Lysed red blood cells Trypticase Soy Agar or Nutrient agar Hemolytic CAP Chocolate agar X factor (hemin) V factor or NAD ( nicotinamide adenine dinucletide, coenzyme) {Nonselective Differential agar} Nonhemolytic PEA Phenylethyl {Selective & Differential agar} Gram (-) inhibitor {Selective agar} ✔ Staphylococcus sp.