IDF International Symposium -Lactose and its Derivatives Analytical Methods for Lactose Quantification in Milk and Milk Products Rachid Kouaouci, Ing., M.Sc., Chemist Valacta, Ste-Anne de Bellevue, Québec, Canada Centre d’expertise en production laitière du Québec
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Metodos Para Cuantificacion de Lactosa en Leche (FIL-IDF)
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IDF International Symposium -Lactose and its Derivatives
Analytical Methods for Lactose Quantification in Milk and Milk Products
Rachid Kouaouci, Ing., M.Sc., ChemistValacta, Ste-Anne de Bellevue, Québec, Canada
Centre d’expertise en production laitière du Québec
IDF International Symposium -Lactose and its Derivatives
IDF International Symposium -Lactose and its Derivatives
HPLCIDF 198 │ISO 22622 (FDIS)
Chromatogram of raw milk sample
IDF International Symposium -Lactose and its Derivatives
HPLCIDF 198 │ISO 22622 (FDIS)
Chromatogram of raw milk sample with added lactulose
IDF International Symposium -Lactose and its Derivatives
HPLCIDF 198 │ISO 22622 (FDIS)
Chromatogram of lactose-reduced milk sample
IDF International Symposium -Lactose and its Derivatives
HPLCIDF 198 │ISO 22622 (FDIS)
Chromatogram of raw milk sample with added sucrose and fructose
IDF International Symposium -Lactose and its Derivatives
HPLCIDF 198 │ISO 22622 (FDIS)
Advantages:Direct methodDifferentiation between carbohydratesAutomated methodReference methodFlexibility
Disadvantages:Costs
IDF International Symposium -Lactose and its Derivatives
HPLCIDF 198 │ISO 22622 (DIS)
IDF International Symposium -Lactose and its Derivatives
HPLC
IDF 198 │ISO 22622 (DIS)
IDF International Symposium -Lactose and its Derivatives
HPLCIDF 198 │ISO 22622 (DIS)
IDF International Symposium -Lactose and its Derivatives
HPLCIDF 198 │ISO 22622 (DIS)
IDF International Symposium -Lactose and its Derivatives
HPLCIDF 198 │ISO 22622 (DIS)
IDF International Symposium -Lactose and its Derivatives
HPLCIDF 198 │ISO 22622 (DIS)
IDF International Symposium -Lactose and its Derivatives
REFERENCES
• Brons, C, and Olieman, C, 1983. Study of the high performance liquid chromatography separation of reducing sugars applied to the determination of lactose in milk, J. Chromato., 259, 79.
• IDF 141C:2000 -Whole Milk- Determination of milkfat, protein and lactose content, Guidance on the operation of Mid-infrared instruments.
• IDF 79-1:2002│ISO 5765-1- Dried milk, dried ice-mixes and processed cheese- Determination of lactose content- Part 1: Enzymatic method utilizing the glucose moiety of the lactose.
• IDF 79-2:2002│ISO 5765-2- Dried milk, dried ice-mixes and processed cheese- Determination of lactose content- Part 2: Enzymatic method utilizing the galactose moiety of the lactose.
• IDF 106:2004│ISO 5548- Caseins and caseinates- Determination of lactose content- Photometric method.
• Official Methods of Analysis, 16th Ed., 4th Revision, 1998, AOAC INTERNATIONAL, Gaithersburg, MD, methods 896.01, 984.15, 930.28 and 972.16.
• Nickerson, T. A., Vujicic, I. F., and Lin, A. Y, 1976. Colorimetric estimation of lactose and its hydrolytic products. J. Dairy Sci., 59, 386.
IDF International Symposium -Lactose and its Derivatives