MEMS design and Micro-fabrication L MML SU -8 M old PD M S replication M IP Reactor M icropump Microvalve (a) (b) (c) (d) Heater Tem p. Sensor (e) 200µm 50µm 2-Dimensional SPR Detection System Integrated 2-Dimensional SPR Detection System Integrated with Molecular Imprinting Polymer Microarrays with Molecular Imprinting Polymer Microarrays Using Microfluidic Technology Using Microfluidic Technology Kuo-Hoong Lee Kuo-Hoong Lee , Yuan-Deng Su, Shean-Jen Chen and Gwo-Bin Lee , Yuan-Deng Su, Shean-Jen Chen and Gwo-Bin Lee Department of Engineering Science, National Cheng Kung University, Tainan, Taiwan 701 Department of Engineering Science, National Cheng Kung University, Tainan, Taiwan 701 This study reports a novel microfluidic chip integrated with arrayed molecular imprinting polymer (MIP) films for surface plasmon resonance (SPR) phase imaging of specific bio-samples. The SPR imaging system uses a surface-sensitive optical technique to detect two-dimensional spatial phase variation caused by bio-molecules absorbed on a sensing surface composed of highly-specific MIP films. The developed system has a high resolution and a high-throughput screening capability and has been successfully applied to the analysis of multiple bio-molecules without the need for additional labeling in long-term measuring. Simplified fabrication process of the SPR/MIP microfluidic chip. (a) SU-8 molding and PDMS casting fabrication process; (b) Spin-coating of MIP films and polymerization process; (c) Temperature sensor and heaters fabricated by using lift-off technique. A novel SPR/MIP microfluidic chip integrated with arrayed MIP films for SPR phase imaging of specific bio- samples was developed. Multiple MIP films could be used for highly- sensitive, highly-specific bio-sensing. The development of the SPR/MIP microfluidic chip can be promising for nano- sensing applications and can detect bio-samples with a low molecular weight. The temperature control system can heated up bio-samples to 37 °C within 20s and kept them at a uniform temperature. A SPR/MIP microfluidic chip comprising microchannels, micropumps/microvalves, micro-heaters and temperature sensors coupled with a 2-D SPR imaging system was developed. Micropumps were used to automate the sample injection. A micromachine- based temperature control module comprised of micro- heaters and a temperature sensor was used to maintain the temperature during measurement. 0 50 100 150 0 0.002 0.004 0.006 0.008 0.01 Tim e (m in) SPR angleshift (deg) Flowed progesterone sam ple 0 50 100 150 0 0.002 0.004 0.006 0.008 0.01 Tim e (m in) SPR angleshift (deg) Flowed progesterone sam ple Arrived saturation W ashed with ethanol The detection kinetics of 50 μM progesterone. Reaction procedure (0 ~ 21 min : ethanol, 21 ~ 126 min : ethanol + 50μM progesterone, 126 min ~ : ethanol). (a) SPR phase interference image and (b) phase reconstructed image when ethanol flows through the arrayed MIP films. The relationship between the pumping rate and the driving frequency. prism Slide(SF-11) Au (47.5 nm ) Outlet Inlet θ 1 CCD He-N e laser M icropump Microchannel Arrayed M IP Heater Tem p. Sensor prism Slide(SF-11) Au (47.5 nm ) Glass Outlet Inlet θ 1 CCD He-N e laser M icropump Microchannel Arrayed M IP Heater Tem p. Sensor SU -8 m olding PD M S replication Si PD M S bonding Cleaning PD M S release/Viaholeform ation Sputtering 47.5nm Au Thiolgroup m odification M IP spin coating and polym erization Lithography Ptdeposition PR lift-off Au lead deposition Assem bly (a) (b) (c) SU -8 m olding PD M S replication Si PD M S bonding Cleaning PD M S release/Viaholeform ation Sputtering 47.5nm Au Thiolgroup m odification M IP spin coating and polym erization Slide Lithography Ptdeposition PR lift-off Au lead deposition Glass Assem bly Assem bly (a) (b) (c) 6 cm Heater Tem p. Sensor 4cm Glass M icropump Microvalve PD M S layer1 & layer2 PD M S layer3 Microchannel Arrayed M IP film s Inlet Outlet 6 cm Heater Tem p. Sensor 4cm Glass M icropump Microvalve PD M S layer1 & layer2 PD M S layer3 Microchannel Arrayed M IP film s Inlet Outlet Microchannel Micropump/valve Inlet Outlet Microchannel Micropump/valve Inlet Outlet Heater Tem p. sensor G old film Microchannel Micropump/valve Inlet Outlet Microchannel Micropump/valve Inlet Outlet PD M S #2 PD M S #3 PD M S #1 Heater Slide (SF 11) - Tem p. sensor G old film (a) Schematic illustration of the arrayed SPR/MIP microfluidic chip (b) Cross- sectional view showing that three layers of PDMS could be used to transport samples from inlet to outlet through the arrayed MIP films. Freq.(H z) Pumpingrate(ul/min) 0 5 10 15 20 10 20 30 40 50 25 psi 20 psi 15 psi 10 psi Tim e(s) Tem perature( o C) 0 25 50 75 100 25 30 35 40 45 SEM images of the SU-8 molds (a and c) and PDMS replicas (b and d) of the arrayed MIP reactors and micropumps/valves. (e) the temperature sensor and heater. Design Conclusions Results Fabrication Abstract The authors gratefully acknowledge the financial support provided to this study by the MOE Program for Promoting Academic Excellence of Universities (Grant number EX- A-91-E-FA08-1-4). Acknowledgements (a ) (b ) (a) (b) 2006