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Supplementary Information
Supplementary information is provided in three files: a word file containing materials and methods,
phylogenetic analysis results, legend to supplementary figures 1, 2, 3, 4, 5, 6, 7 and 8;
supplementary tables 1, 2 and 3, sequences of Skeletonema marinoi transcripts of the prostaglandin
pathway reported in the main text; a word file with the sequences used in the phylogenetic analysis;
eight pdf file containing the supplementary figures 1, 2, 3, 4, 5, 6, 7 and 8.
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Supplementary information: file 1.
Content: materials and methods, phylogenetic analysis results, legend to supplementary figures 1, 2, 3, 4, 5, 6, 7 and 8; supplementary tables 1, 2 and 3; sequences of Skeletonema marinoi transcripts of the prostaglandin pathway reported in the main text.
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Materials and Methods
Bioinformatic identification of the Prostaglandin pathway
Transcriptome sequencing of Skeletonema marinoi stain FE7 and FE60 (MMETSP1039 and
MMETSP1040 respectively) were obtained thanks to the Marine Microbial Eukaryote
Transcriptome Sequencing Project initiative, by the National Centre for Genome resources (NGR)
(Santa Fe, New Mexico, USA), supported by the Betty and Gordon Moore Foundation
(www.moore.org). Sequences are now freely available in the public database CAMERA
(http://camera.crbs.ucsd.edu/mmetsp/list.php) and iMicrobes interactive query tool for microbial
data (http://data.imicrobe.us/sample/view/1867 http://data.imicrobe.us/sample/view/1868).
To identify expressed metabolic pathways, Skeletonema marinoi FE7 and FE60 contigs were
submitted to the Kyoto Encyclopaedia of Genes and Genome (KEGG) Automatic Annotation
Server (KAAS, http://www.genome.jp/tools/kaas/) as “Complete or Draft Genome” using bi-
directional best hit assignment method and adding to the default organisms the following others:
Phaeodactylum tricornutum, Thalassiosira pseudonana, Ostreococcus lucimarinus, Ostreococcus
tauri and Cyanidioschyzon merolae. Enzymes involved in prostaglandin synthesis were found
annotated in map 0590 relative to arachidonate metabolism (Supplementary Figure S1). The protein
sequence annotated as prostaglandin transporter was found exploring the annotation list obtained
using Blast2go PRO software (http://www.blast2go.com/b2ghome) with default parameters.
Phytoplankton cultures
The two Skeletonema marinoi strains, FE7 and FE60, were isolated from phytoplankton samples
collected during diatom blooms in the northern Adriatic Sea in 1997 and 2005, respectively
(Gerecht et al., 2011). Three replicate cultures of each clone, with initial cell concentrations of 5000
cells/mL, were grown in f⁄2-medium (Guillard, 1975) in 10 lt polycarbonate bottles under gentle
bubbling with sterile-filtered ambient air and kept in a climate chamber at 20°C on a 12:12 light:
dark (L:D) cycle at 100 µmol photons m-2 s-1. Culture growth was followed in each replicates by
daily cell enumeration in a Sedgewick-Rafter counting chamber (Hausser Scientific, Horsham, PA, 3
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USA), up to the 11th day from the inoculation. All cultures were harvested always at the same time
of the day in order to avoid interference due to circadian variability. Samples were collected on day
4, 7 and 11 representing exponential, stationary and senescent phases of the growth curve,
respectively (Supplementary figure S2). Cultures were concentrated by centrifugation in a cooled
centrifuge at 2753g for 15 min with a swing-out rotor (DR 15P, Braun Biotechnology International,
Allentown, PA, USA), immediately frozen in liquid nitrogen, and kept at -80°C until analysis.
Silica starvation experiment were conducted in triplicates as described in Orefice et al. (Orefice et
al., 2015).
RNA extraction and reverse transcription
Total RNA was extracted from 500 ml of cultures harvested at day 4 and 300 ml of cultures
harvested at days 7 and 11. Each pellet was treated following the procedure described in Barra et al.
(Barra et al., 2013). DNase treatment was carried out using DNAse I recombinant, RNAse-free
(Roche, Basel, Switzerland) method according to manufacturer's protocol to eliminate potential
genomic DNA contamination. Total RNA sample was purified and concentrated using RNeasy
MinElute Cleanup Kit (Qiagen, Venlo, Netherlands) and eluted in 20 μl RNase-free water.
Concentration of the resulting RNA samples was assessed by absorbance at 260 nm (ND-1000
Spectrophotometer; NanoDrop Technologies, Wilmington, DE, USA). The integrity of total RNA
was checked with agarose gel electrophoresis. From each RNA sample 1g was retro-transcribed in
the T100 Thermal cycler (Bio-Rad Laboratories, Hercules, CA, USA) following the manufacturer's
instructions of the sensiFAST™ cDNA synthesis kit (Bioline, Cat. No. BIO-65054).
Primer design and real time quantitative PCR
Transcript of interest were selected considering the annotation of the putative protein sequences
reported in the transcriptome of S. marinoi strain FE7 from CAMERA: MMETSP1039-20121108|
4563_1 Prostaglandin G/H synthase 1/2 (Ptgs 1/2) or cyclooxygenase-1 (COX-1), MMETSP1039-
20121108|5744_1 Prostaglandin E synthase 2 (PtgEs), MMETSP1039-20121108|1431_1 Glutatione
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S-transferase o Prostaglandin H2 D-isomerase (PtgHi or PtgDs), MMETSP1039-20121108|
17278_1 Prostaglandin Transport (PtgT).
Primers, designed using Primer3 program V. 0.4.0 (Untergasser et al., 2012; Koressaar and Remm,
2007), are:
COX-1: Ptgs_F: GGGTGGGATGATGAACGTGT; Ptgs_R: AAGGACCTGGCTCGACTTTG;
PtgEs: PtgEs_F: GAGTTGAAACCATGGTCAGGAG; PtgEs_R:
TGTGTATCAAGTAGCGCATCG; PtgDs or PtgHi: PtgHi_F: AAAAGAATGGCGGAGTGGGA;
PtgHi_R: GTCGCCACAATACCCGCATA; for PtgT: PtgTr_F:
GGAGAGCATTGGAGTCAGCA; PtgTr_R: TTCGCCACCCTCTGAAATGT.
Each sequence was initially tested by standard PCR. Reactions were carried out in 25 μl volume
with 2,5 μl of 10× PCR reaction buffer (Roche, Basel, Switzerland), 2,5 μl of 10 × 2 mM dNTP, 0.2
μl of 5 U/μl Taq (Roche, Basel, Switzerland), 1,25μl 10 of each oligo, 1 μl of cDNA templates
and nuclease-free water up to 25 μl. The PCR program consisted of a denaturation step at 95 °C for
3 min, 40 cycles at 95 °C for 30 s, 53 °C 30s, 72 °C for 30s, and a final extension step at 72 °C for 7
min. Amplified PCR products were analysed by agarose gel electrophoresis. The resulting bands
were excised from the gel and extracted according to the GenElute Gel Extraction Kit protocol
(Sigma-Aldrich, St. Louis, MO, USA). Sequences were obtained by BigDye Terminator Cycle
Sequencing Technology (Applied Biosystems, Foster City, CA, USA) and purified using the
Agencourt CleanSEQ Dye terminator removal Kit (Agencourt Bioscience Corporation, Beverly,
MA, USA) in automated robotic station Biomek FX (Beckman Coulter, Pasadena, CA, USA).
Products were analysed on the Automated Capillary Electrophoresis Sequencer 3730 DNA
Analyser (Applied Biosystems, Foster City, CA, USA). Alignments were performed with BioEdit
software V. 7.0.5.3 (http://www.mbio.ncsu.edu/BioEdit/bioedit.html).
Selected reference genes were ribosomal protein small subunit 30S (RPS), actin (ACT) and
ubiquitin (UB) which were the best reference genes in Orefice et al. (Orefice et al., 2015).
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Reverse transcription-quantitative PCR (RT-qPCR) experiments were performed in MicroAmp
Optical 384-Well reaction plate (Applied Biosystems, Foster City, CA, USA) with Optical
Adhesive Covers (Applied Biosystems, Foster City, CA, USA) in a Viia7 Real Time PCR System
(Applied Biosystem, Foster City, CA, USA). Five serial dilutions of mixed cDNAs were used to
determine primer reaction efficiency (E= 10−1/slope) that for each oligo couple was two. The PCR
volume for each sample was 10μl, with 5μl of Fast Start SYBR Green Master Mix with ROX
(Roche, Basel, Switzerland), 1μl of cDNA template (1 to 5 dilution each template) and 1μl of 10M
oligo mix (forward and reverse). Program reaction used was: 95°C for 20s, 40 cycles of 95°C for 1s
and 60°C for 20s. The program was set to reveal the melting curve of each amplicon from 60°C to
95°C, and read every 0.5°C. Single peaks for all genes confirmed gene-specific amplification and
the absence of primer-dimers. All RT-qPCR reactions were carried out in triplicate to capture intra-
assay variability. Each assay included three no-template negative controls for each primer pair.
The expression levels of each gene of interest relative to the most stable reference genes were
calculated by using the REST tool (Relative Expression Software Tool) (Pfaffl et al., 2002). The
evaluation of gene expression along the growth curve of S. marinoi, was calculated considering the
exponential phase at the fourth day of the growth curve as the reference condition.
Data are represented in graph as log2 x-fold difference in the expression level of the analysed genes
in the stationary (Day7) and senescent (Day11) phases compared to the control exponential phase
(Day4). Results of silica starvation experiment are reported as log2 x-fold difference in the
expression level of the analysed genes for each growth phase under silica starvation with respect to
the corresponding phase in standard culturing conditions (control: complete medium). Relative
expression ratios above two fold were considered significant. Statistical analysis was performed
using the Pair Wise Fixed Reallocation Randomisation test by REST.
Prostaglandin extraction
500 ml of cell culture from day 4 and 300 ml of cell culture from days 7 and 11 were harvested by
centrifugation at 2753g for 15 minutes. The pellets were re-suspended in 1 ml PBS per gr wet
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weight, added with 1µg of Prostaglandin E2-d4 (CAYMAN CHEMICAL, Michigan, USA) as
internal standard and sonicated with 1-minute pulse, 30 seconds on ice and another 30 seconds of
pulse at 20% of maximum intensity. After sonication, an aliquot of 50 l was taken to measure
proteins concentration by Bradford assay (PanReac AppliChem, product code A6932). Samples
were left 10 minutes at room temperature. To precipitate proteins, samples were added with 4
volumes of ethanol and left 5 minutes at 4°C. Precipitated proteins were removed by centrifugation
at 3000g for 10 minutes. After ethanol evaporation under nitrogen, samples were acidified with 4
volumes of 1M acetate buffer pH 4 and centrifuged 15 minutes at 4200g. Clean supernatant was
fractionated by using an automated procedure on a GX-271 ASPEC Gilson
apparatus equipped with a TRILUTION® LH Software (Gilson, Middleton, WI,
USA). C18 columns (Aspec C18 6 mL/500 mg. Gilson, Middleton, WI, USA) were
activated with 5 ml of methanol and equilibrated with 10 mL of distilled water. Elution
was performed after a first washing step with 5 mL 100% H2O using 5ml ethyl
acetate-1% methanol. The ethyl acetate-methanol fraction was dried under nitrogen and saved until
the chemical analysis.
LC/MSMS analysis
Samples were resuspended in 1ml methanol and analysed by using a 4000QTrap mass spectrometer
(Applied Biosystems, Toronto , Canada), a QqLIT instrument, working in negative ion mode and
coupled to a 1100 nanoHPLC system (Agilent Technologies, Waldbronn, Germany). The
prostaglandins were separated by using a micro C18 column (10 cm x 1,0mm, 5µm). The mobile
phase was generated by mixing eluent A (0.1% Acetic Acid) and eluent B (acetonitrile/isopropanol
50/50) and the flow rate was 30 nl/min. Starting condition was 20% to 95% B in 15 minutes.
Tandem mass spectrometry was performed using a turbo ion spray source operated in negative
mode, and the multiple reaction monitoring (MRM) mode was used for the selected analytes. For
the detection of metabolites, the Turbo ion spray source was operated as follows: curtain gas 20psi,
GS 1/2 50/50psi, ion spray voltage -5500V , DP, -50 V; Dwell 25ms and temperature of 550°C. MS
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were carried out on a quadrupole MRM mode for identification and quantification. MRM was
performed by the detection of a unique product ion arising from collision-induced fragmentation of
the deprotonated selected parent compounds (Wang et al., 2014; Yang et al., 2009) after proper
optimization of mass spectral parameters as reported in Supplementary Table 1 and Supplementary
Figure 3 and 4.
The amount of prostaglandins quantified as described above, was normalized by protein content of
each sample.
Phylogenetic analyses
The COX-1, PTGE Synthase, PTGH Isomerase and PTG Transporter sequences from the other
diatom species were obtained as follow: the file with the peptide sequences of each diatom species
was downloaded from the MMETSP website (http://data.imicrobe.us/project/view/104). Each
peptide file was indexed (following NCBI instructions:
http://www.ncbi.nlm.nih.gov/books/NBK1763/) and searched using the corresponding Skeletonema
marinoi clone FE7 sequence as a query in a blastp search with default parameters. Sequences with
highest similarity were blasted against the non-redundant (NR) NCBI database to verify the identity
and checked for their annotation in the corresponding MMETSP project swiss-prot table. Outgroup
sequences were selected from the blastp results of each Skeletonema marinoi strain FE7 protein
analyzed in the prostaglandin pathway against the NCBI non redundant sequences database
(https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE=Proteins ).
Sequences were visualized and aligned using BioEdit Sequence Alignment Editor (Hall, 1999). A
first automatic alignment was performed using the ClustalW algorithm implemented in BioEdit,
then the alignment was manually refined. Bayesian analyses were performed on the alignment using
MrBayes (Ronquist and Huelsenbeck, 2003) computer program. All the settings for each analysis
are listed in the figure 2 legend. For Bayesian analyses, two parallel and totally independent
Markov Chain Monte Carlo (MCMC) runs were carried out on data matrices. Four chains (three hot
and one cold) drove each analysis. The number of generations and the split frequency standard
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deviation for each analysis are reported in figure 2 legend. Sampling frequency was set every 500
generations. The analyses were forced to jump among the evolutionary models for protein sequence
alignments implemented in the software. The first 25% of the samples from the cold chain were
discarded in order to stabilize the algorithm, reduce the variability among results and have a more
robust analysis. Consensus tree with posterior probability (PP) of each node and branch length are
reported after a 50% majority-rule consensus. Phylogenetic trees were visualized and edited in the
FigTree (Tree Figure Drawing Tool Version 1.4.2) software (http://tree.bio.ed.ac.uk/).The complete
list of sequences used in the present work for phylogenetic analyses are listed in Supplementary
table 3. The sequences from strain SkelB that were annotated as Skeletonema dohrnii in MMETSP
database were changed to S. marinoi after ribosomal sequence identification. Sequences ID were
left unchanged in the fasta sequences list attached at the end of this supplemental information file.
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Results
Effect of nutrient limitation on prostaglandin enzymes gene expression
Silica starvation experiment (36 μM Si(OH)4) revealed a large variability among replicates and no
significant differences were evident in the expression levels of prostaglandin synthesizing enzymes
(Supplementary Figure S5).
The MMETSP database includes transcriptomic data of different diatom species and clones grown
in many different conditions: nutrient limitation (Silica, Iron, Nitrate, Phosphorus), high or low
CO2, changes in light/dark cycle, freezing temperature, nocodazole treatment, different nitrogen
sources such as glutamate,urea, ammonium or nitrate. We used this information to analyze the
relative presence of the three enzymes of the prostaglandin pathway (Supplementary Table 2).
Results highlight a large variability of COX-1 expression (reported as Fragments Per Kilobase of
transcript per Million mapped reads, FPKM) among species and among different growth conditions
for the same species. For example, COX-1 expression in Thalassiosira gravida, clone GMp14c1,
seems to be affected by nitrogen limitation but not by phosphorus with respect to the control
condition. Skeletonema marinoi clone SkelA, contrary to our clones, seems to be affected by silica
deprivation, with COX-1 expression being almost doubled compared to the control condition. On
the other hand, COX-1 expression seems to be affected by silica limitation in Chaetoceros
neogracile, by iron deprivation in Chaetoceros debilis and by nitrogen, phosphorus and CO2 in
Chaetoceros affinis.
Detailed results of the phylogenetic analyses on Prostaglandin D Synthase (PTGDS) and Prostaglandin transporter (PTGT)
In addition to information given in the main text, here we report detailed description of the
phylogenetic analysis performed on two of the protein sequences involved in the prostaglandin
pathway.
PTGD Synthase
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The Bayesian phylogeny produced with PTGD Synthase alignment contained 230 sequences from
diatoms and other organisms (Supplementary Figure S7). The protein sequences were very variable
and in fact after 10 million generations, the split frequency standard deviation did not go below 0.04
and the log likelihood did not change over at least five million generations. This tree showed at least
nine variably supported big clades (PP from 0.53 to 1.00); five out of them contained only diatom
sequences. The remaining four clades contained at least one outgroup sequence, some of which
annotated as Glutathione S-transferase 4, Glutathione S-transferase-like protein, Glutathione-
requiring prostaglandin D synthase or Glutathione S-transferase. The other outgroup sequences in
fact cluster in unresolved branches or in supported but not resolved small clades. What is worth of a
note is that all but one clades contained both centric and pennate diatoms; this can corroborate the
hypothesis that the nine clades in fact cluster different proteins, present in both diatom classes. This,
together with the low level of resolution of the phylogeny, suggests that the sequences retrieved by
BLAST in MMETSP and in NCBI databases for diatoms and other organisms in fact share a certain
identity and contain similar domains, but can belong to different protein families. Conversely,
PTGD Synthase can have evolved quite differently in different organisms and also in diatoms
different proteins can serve the same function or be involved in different conditions. More
functional studies are required to disentangle this issue.
PTG transporter
PTGT sequence was annotated in the transcriptomes of our strains as prostaglandin transporter.
However, blastp of its protein sequence in the NCBI database retrieved similarity with the broad
ABC transporter family which include less specific transporters. For this reason, Bayesian analysis
of the PTGT sequence was restricted only to diatom sequences that showed a low e-value indicating
high rate of similarity. The analyses showed four main clades indicated by different shades in
Supplementary Figure 8. In this tree, some diatom genera are represented in all the clades, while
some others appear in only one (Cyclotella, Amphora, Coscinodiscus, Astrosyne, Cyclophora,
Nitzschia, Attheya, Striatella), two (Staurosira, Grammatophora, Dactyliosolen, Odontella,
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Aulacoseira, Thalassiotrix, Minutocellulus) or three clades (Amphiprora, Rhizosolenia,
Asterionellopsis, Licmophora, Thalassionema, Stephanopyxis, Proboscia, Letpocylindrus). Among
the genera that appear in only one clade, no clear taxonomic pattern can be identified because in
clade A two centric and three pennate diatoms are recorded as single appearances; while in clade D,
one centric and two pennates were recorded. What is worth noting is that single occurrences were
recorded only in clades A and D (that are the widest in the tree) and that pennate genera are the
most represented with five genera against 3 centric ones. Among the double and triple occurrences,
centric diatoms are the most represented, with five genera against two pennate for the former; and
six centric against two pennate genera for the latter. All the double and triple occurrences have at
least one representative in clades A and D (apart from Minutocellulus with sequences clustering in
clades A and C and Asterionellopsis not recorded in clade D). From this analysis of clustering
pattern it emerged that likely transporter proteins clustering in clades A and D are the most spread
among diatoms (no single occurrences were recorded in clades B and C) possibly revealing a
subordinate hierarchical level of transporter proteins B and C. No identification of subcellular
localization was performed on the sequences because transcripts usually miss the specific signal
peptide or were truncated.
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References
Barra L, Ruggiero MV, Sarno D, Montresor M, Kooistra WHCF. (2013). Strengths and weaknesses of microarray approaches to detect Pseudo-nitzschia species in the field. Environ Sci Pollut Res Int 20: 6705–6718.
Gerecht A, Romano G, Ianora A, d’Ippolito G, Cutignano A, Fontana A. (2011). Plasticity of Oxylipin Metabolism Among Clones of the Marine Diatom Skeletonema Marinoi (bacillariophyceae)1. J Phycol 47: 1050–1056.
Guillard RRL. (1975). Culture of Phytoplankton for Feeding Marine Invertebrates. In: Smith WL, Chanley MH (eds). Culture of Marine Invertebrate Animals. Springer US, pp 29–60.
Koressaar T, Remm M. (2007). Enhancements and modifications of primer design program Primer3. Bioinforma Oxf Engl 23: 1289–1291.
Orefice I, Lauritano C, Procaccini G, Ianora A, Romano G. (2015). Insights into possible cell-death markers in the diatom Skeletonema marinoi in response to senescence and silica starvation. Mar Genomics 24 Pt 1: 81–88.
Pfaffl MW, Horgan GW, Dempfle L. (2002). Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 30: e36.
Ronquist F, Huelsenbeck JP. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574.
Hall TA. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, Remm M, et al. (2012). Primer3--new capabilities and interfaces. Nucleic Acids Res 40: e115.
Wang Y, Armando AM, Quehenberger O, Yan C, Dennis EA. (2014). Comprehensive ultra-performance liquid chromatographic separation and mass spectrometric analysis of eicosanoid metabolites in human samples. J Chromatogr A 1359: 60–69.
Yang J, Schmelzer K, Georgi K, Hammock BD. (2009). Quantitative Profiling Method for Oxylipin Metabolome by Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry. Anal Chem 81: 8085–8093.
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Legend to Supplementary Figure 1, 2, 3, 4, 5, 6, 7 and 8
Supplementary Figure 1: Arachidonic acid metabolism reference pathway (Map 0590).
The map shows the metabolic pathways originating from the arachidonic acid, including the
prostaglandin pathway. Enzymes are identified by their code numbers (EC) enclosed in rectangular
boxes. Red arrows highlight the prostaglandin pathway. Green ovals indicate gene product
annotated in the Skeletonema marinoi strain FE7 transcriptome and blue rectangles the gene product
annotated in FE60 strain.
Supplementary Figure 2: Skeletonema marinoi growth curves.
Growth curve of Skeletonema marinoi strain Fe7 (A) and strain FE60 (B) from day 0 to day 11.
Day 4, 7 and 11, have been taken as representative point of the exponential, stationary and
senescent phases of the growth curve, respectively. Day 4 was considered the control phase, as it
represents the health phase of a diatom culture.
Supplementary Figure 3: MRM chromatogram.
MRM chromatogram for diatom sample displaying transitions for all targeted prostaglandins.
Supplementary Figure 4: MRM chromatogram of selected analytes.
MRM chromatogram only showing some detected PGs. The specific MRM transitions for
monitoring the different analytes are indicated as an example. The deuterated PG used as internal
standard is also reported.
Supplementary Figure 5: Expression levels of the prostaglandin enzymes in Skeletonema
marinoi (Clone FE7) under silica starvation.
Expression levels of enzymes responsible for the PGs synthesis and of the prostaglandin transporter
measured by Real Time qPCR in exponential, stationary and senescent phases of growth under
silica starvation. Results were analysed with the REST software and reported as 2log expression
ratio on the standard growth conditions (control) ±SD. Statistical analysis (N=3) was performed
using the Pair Wise Fixed Reallocation Randomization test by REST. Relative expression ratios
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above two fold were considered significant. Results are means of three technical replicates for each
biological replicate (N=3).
Supplementary Figure 6: Skeletonema marinoi-COX1 alignment.
Skeletonema marinoi COX1 sequence (highlighted in yellow) alignment with representative COX
sequences from vertebrate and invertebrate organisms. The alignment of Sm_COX-1 showed a
percent of identity ranging from about 36% to 24% having the highest similarity with cyanobacteria
followed by mammals, corals, teleosts and finally red algae. Amminoacidic residues Gln203 and
His388 involved in peroxidase activity and residue Tyr385, crucial to initiate cyclooxygenase
activity, were conserved, similarly to the red algae, while the region containing the amminoacidic
residue Arg120, which is the site that interacts with the substrate, was poor conserved.
Representative domains are highlighted in red boxes following the Ovis aries (highlighted in pink)
COX sequence positions. Important amminoacidic residues are highlighted in blue.
Supplementary Figure 7: PTGD Synthase phylogenetic tree.
Bayesian mid-point routed phylogenetic trees of PTGD Synthase proteins (10,000,000 generations,
split frequency standard deviation = 0.04) from pennate (blue) and centric (red) diatoms. Non-
diatom sequences are reported in black. Sequences used for phylogenetic analyses are listed in
Supplementary Table S3. The tree was built on scale in the branch length (scale bars reported).
Sequences are identified by the species name and annotation (where applicable), followed by their
MMETSP ID deprived of the taxon ID for simplicity or GenBank accession number. Posterior
probability (PP) values are reported at the nodes. Branch coloring refers to PP, color code is
reported in figure. The nine main clades are identified by letters (see Figure 2c in the main text)
highlighted with vertical bars on the right hand side of the picture. Extended clusters 10 and 11 are
also highlighted.
Supplementary Figure 8: PTG transporter phylogenetic tree.
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Bayesian mid-point routed phylogenetic tree (5,000,000 generations, split frequency standard
deviation = 0.01) of proteins from pennate (blue) and centric (red) diatoms. The tree was built on
scale in the branch length (scale bar reported). Sequences are identified by species name and
MMESP ID deprived of the taxon ID for simplicity. Four main clades (see text) are highlighted in
different colors. Posterior probability (PP) values are reported at the nodes. Branch coloring refers
to PP, color code is reported in figure.
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Supplementary Table 1: Prostaglandin metabolites mass spectral parameters (LC/MSMS data)
Abbreviation Name Pathway Precursor fatty acid
Parent ion
Product Ions
collision energy (V)
PGE1 Prostaglandin E1 COX ETE 353 191 -28235 -29
PGD1 Prostaglandin D1 COX ETE 353 317 -30163 -29
PGF1 Prostaglandin F1 COX ETE 355 311 -24293 -36
6k PGE1 6-keto-prostaglandin E1 COX ETE 367 163 -30331 -36
PGE2 Prostaglandin E2 COX AA 351 235 -25271 -35
PGD2 Prostaglandin D2 COX AA 351 271 -24233 -35
PGF2 Prostaglandin F2 COX AA 353 309 -32193 -35
PGA2 Prostaglandin A2 COX AA 333 189 -30271 -20
PGB2 Prostaglandin B2 COX AA 333 175 -25235 -30
PGJ2 Prostaglandin J2 COX AA 333 251 -28189 -30
PGK2 Prostaglandin K2 COX AA 349 287 -31249 -28
11 PGF2 11 Prostaglandin F2 COX AA 353 299 -25335 -30
15d PGA2 15-deoxy-Prostaglandin A2 COX AA 315 233 -23255 -30
15d PGD2 15-deoxy-Prostaglandin D2 COX AA 333 203 -28271 -26
15d PGJ2 15-deoxy-Prostaglandin J2 COX AA 318 217 -30203 -30
15k PGD2 15-keto-Prostaglandin D2 COX AA 349 287 -24235 -18
15k PGE2 15-keto-Prostaglandin E2 COX AA 349 283 -27235 -22
15k PGF2 15-keto-Prostaglandin F2 COX AA 351 289 -30219 -30
dhk PGE2 13,14-dihydro-15-keto-Prostaglandin E2 COX AA 351 315 -26
207 -28
dhk-PGD2 13,14-dihydro-15-keto-Prostaglandin D2 COX AA 351 333 -24
175 -35
dh-PGF2 13,14-dihydro Prostaglandin F2 COX AA 355 275 -32
283 -22
dhomo-PGF2 dihomo-Prostaglandin F2 COX AA 381 301 -24337 -24
dihomo_PGJ2 dihomo-Prostaglandin J2 COX AA 361 284 -17299 -28
PGE3 Prostaglandin E3 COX EPA 349 313 -15269 -24
PGD3 Prostaglandin D3 COX EPA 349 233 -28269 -24
PGF3 Prostaglandin F3 COX EPA 351 147 -24193 -26
17
357
358
359
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Page 18
Supplementary table 2: presence/absence of complete or partial prostaglandin pathway in all available MMETSP transcriptomes of diatom species grown in different conditions.
MMETSP ID Genus Species Clone Treatment COX1
FPKM
PtgE Synthas
e
PtgH isomerase
Ptg Transporte
r
1065 Amphiprora paludosa CCMP125 - - + + +
0724 Amphiprora sp CCMP467 Exponential - + + +
0727 Amphiprora sp CCMP467 Nocodazole - - - +
0725 Amphiprora sp CCMP467 SiLim - + + +
0316 Amphora coffeaeformis CCMP127
f/2 + silicate,
adjusted to 1 mM
nitrate and amended with 200
µM acetate
- - - +
0317 Amphora coffeaeformis CCMP127
f/2 + silicate, but
without nitrate
- - - -
0318 Amphora coffeaeformis CCMP127
f/2 + silicate,
adjusted to 1 mM nitrate
- - + -
1394 Asterionellopsis glacialis CCMP1581 - + + + +
0713 Asterionellopsis glacialis - - - + + +
0705 Asterionellopsis glacialis CCMP134 Exponential - - - -
0708 Asterionellopsis glacialis CCMP134 Nocodazole - - - -
0707 Asterionellopsis glacialis CCMP134 N-limited + 7305,03 - + +
0706 Asterionellopsis glacialis CCMP134 Si-limited + 8052,7 - + -
0418 Astrosyne radiata 13vi08-1A - - - + +
1449 Attheya septentrionalis CCMP2084 - - + + +
1064 Aulacoseira subarctica CCAP1002/5 - + + + +
0785 Bolidomonas pacifica CCMP1866 - - + + -
0088 Chaetoceros affinis CCMP159 Replete-ModernCO2 + 22383 - + -
0090 Chaetoceros affinis CCMP159 -N-ModernCO2 + 43066 - + +
0091 Chaetoceros affinis CCMP159 -P-ModernCO2 + 7994,5 + + +
18
360361
3536
Page 19
0092 Chaetoceros affinis CCMP159 Replete-FutureCO2 + 2526,8 - + -
1435 Chaetoceros brevis CCMP164 - - - + -
0716 Chaetoceros curvisetus Exponential - - - -
0717 Chaetoceros curvisetus Exponential + + + +
0718 Chaetoceros curvisetus Exponential - + + +
0719 Chaetoceros curvisetus Exponential - - - -
0149 Chaetoceros debilis MM31A-1 CTR +21207,5
- + +
0150 Chaetoceros debilis MM31A-1 -Fe +10718,8
+ + +
1447 Chaetoceros dichaeta CCMP1751 - - + + +
1336 Chaetoceros neogracile RCC1993 - + + + +
0751 Chaetoceros neogracile CCMP1317 Exponential + 2887 - + +
0753 Chaetoceros neogracile CCMP1317 NLim + 2311,38 + - -
0754 Chaetoceros neogracile CCMP1317 Nocodazole + 5420,1 - + +
0752 Chaetoceros neogracile CCMP1317 SiLim + 4620 - + +
0200 Chaetoceros sp. clone GSL56 - - + + +
1429 Chaetoceros sp. clone UNC1202 - - + + +
0010 Corethron hystrix 308 - - + + +
0169 Corethron pennatum L29A3 CTR - - + +
0171 Corethron pennatum L29A3 -Fe - - + +
1066 Coscinodiscus wailesii CCMP2513 - - + + +
1442 Craspedostauros australis CCMP3328 - - + + +
0397 Cyclophora tenuis ECT3854 - - + + +
1057 Cyclotella meneghiniana CCMP 338 - + - + +
0017 Cylindrotheca closterium KMMCC:B-181 - - + + +
0580 Dactyliosolen fragilissimus - - + + +
1058 Detonula confervacea CCMP 353 - + + + +
1443 Entomoneis sp CCMP2396 - - + + -
193738
Page 20
1437 Eucampia antarctica CCMP1452 - - + + +
0733 Fragilariopsis kerguelensis L26-C5 Fe LL - - + +
0734 Fragilariopsis kerguelensis L26-C5 Fe SL - - + +
0735 Fragilariopsis kerguelensis L26-C5 -Fe LL - + + -
0736 Fragilariopsis kerguelensis L26-C5 -Fe SL - - + +
0906 Fragilariopsis kerguelensis L2-C3
freezing_temperature (T=-2°C, light=45)
- - - -
0907 Fragilariopsis kerguelensis L2-C3
control growth (T=5°C,
light=100)
+ - - +
0908 Fragilariopsis kerguelensis L2-C3
iron limitation,
T=4°C, light=80
- - + +
0909 Fragilariopsis kerguelensis L2-C3 Darkness, T=5°C - - - -
0009 Grammatophora oceanica CCMP 410 - + - + +
0322 Leptocylindrus apora B651 - - + + +
0321 Leptocylindrus danicus B650 - - - - -
1362 Leptocylindrus danicus CCMP1856 - - + + +
1360 Licmophora paradoxa CCMP2313 - - + + +
1070 Minutocellus polymorphus NH13 - - - + +
1434 Minutocellus polymorphus CCMP3303 - - - - +
1322 Minutocellus polymorphus RCC2270 - - + + +
0744 Nitzschia punctata CCMP561 Exponential - - + +
0745 Nitzschia puncatata CCMP561 SiLim - + - +
0746 Nitzschia puncatata CCMP561 N-limit - - - -
0747 Nitzschia punctata CCMP561 Nocodazole - - + +
0014 Nitzschia sp RCC80 - + + + +
0015 Odontella aurita 1302-5 - + + + +
0160 Odontella sinensis Grunow1884 - + + + +
203940
Page 21
0174 Proboscia alata PI-D3 CTR - - + +
0176 Proboscia alata PI-D3 -Fe - + + +
0816 Proboscia inermis CCAP1064/1 - - + + +
0329 Pseudonitzschia arenisensys B593 - + + + +
0142_2 Pseudonitzschia australis 10ABGLUTAMA
TE as N source
- - + +
0140 Pseudonitzschia australis 10AB NH4 as N source - - + -
0139 Pseudonitzschia australis 10AB NO3 as N source + - - +
0141 Pseudonitzschia australis 10AB UREA as N source - + + +
0327 Pseudonitzschia delicatissima B596 - - + + +
0850 Pseudonitzschia fraudolenta WWA7 Si Replete 390ppm - - - +
0851 Pseudonitzschia fraudolenta WWA7 Si Lim 390ppm - - + +
0852 Pseudonitzschia fraudolenta WWA7 Si Replete 750ppm - + + -
0853 Pseudonitzschia fraudolenta WWA7 Si Lim 750ppm - - - -
1423 Pseudonitzschia heimii UNC1101 - - + + +
- Pseudonitzschia multistriata female at little size - + + +
1061 Pseudonitzschia pungens - + - + -
1060 Pseudonitzschia pungens f cingulata - - + - +
0789 Rhizosolenia setigera CCMP 1694 - - + + +
0013_2 Skeletonema costatum 1716 - + - + +
0562 Skeletonema marinoi SkelB Replete + - - +
0563 Skeletonema marinoi SkelB N-limit - - - +
0578 Skeletonema grethea CCMP 1804 - + - + +
0593 Skeletonema japonicum CCMP2506 - - - + +
1040 Skeletonema marinoi FE60 - - + + +
1039 Skeletonema marinoi FE7 - + + + +
0918 Skeletonema marinoi SkelA Replete + 10270 - - -
214142
Page 22
0920 Skeletonema marinoi SkelA SiLim + 24515 - - -
0320 Skeletonema marinoi SM1012Den-03 NoCuNoSi +
+ + +
0319 Skeletonema marinoi SM1012Hels-07 NoCuNoSi + - - +
1428 Skeletonema marinoi UNC1201 - + - - -
0603 Skeletonema menzelii CCMP793 Replete - - + +
0604 Skeletonema menzelii CCMP793 NLim - - - +
1361 Staurosira complex CCMP2646 - + - - +
0794 Stephanophyxis turris CCMP 815 - - + + +
0800 Striatella unipunctata CCMP2910 - - - + +
1176 Synedropsis recta CCMP1620 - - - + -
0786 Thalassionema frauenfeldii CCMP 1798 - + + + +
0156 Thalassionema nitzschioides L26-B CTR 1 - + + +
0158 Thalassionema nitzschioides L26-B -Fe - - + +
0905 Thalassiosira antarctica CCMP982 control growth - + - +
0902 Thalassiosira antarctica CCMP982 freezing temperature - - + +
0903 Thalassiosira antarctica CCMP982 iron limitation - - + -
0904 Thalassiosira antarctica CCMP982 silica limitation - - + -
0492 Thalassiosira gravida GMp14c1 Replete + 5168,2 - + -
0493 Thalassiosira gravida GMp14c1 NLim + 2093 - - +
0494 Thalassiosira gravida GMp14c1 PLim + 5953,6 - - +
0737 Thalassiosira minuscola CCMP1093 Exponential - - + +
0738 Thalassiosira minuscola CCMP1093 SiLim - - - -
0739 Thalassiosira minuscola CCMP1093 NLim - + + +
0740 Thalassiosira minuscola CCMP1093 Nocodazole - - - -
0970 Thalassiosira oceanica CCMP1005 Replete - + + +
0971 Thalassiosira oceanica CCMP1005 iron limitation - + - +
0972 Thalassiosira oceanica CCMP1005 Silicate Limitation - - - +
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Page 23
0973 Thalassiosira oceanica CCMP1005 Nitrate Limitation - - - -
1067 Thalassiosira puntigera Tpunct2005C2 - - - + +
0404 Thalassiosira rotula CCMP3096 darkcycle - - + -
0403 Thalassiosira rotula CCMP3096 lightCycle - + - +
0910 Thalassiosira rotula GSO102 PLim - - + +
0911 Thalassiosira rotula GSO102 FeLim - - - -
0912 Thalassiosira rotula GSO102 FeLim_2 - - - -
0910 Thalassiosira rotula GSO102 Replete - - - -
1059 Thalassiosira sp. FreshWater - - + + +
1071 Thalassiosira sp. NH16 - - + + +
0878 Thalassiosira weissflogii CCMP1336 20nM Fe - - - -
0879 Thalassiosira weissflogii CCMP1336 60nM Fe - - - -
0880 Thalassiosira weissflogii CCMP1336 20nM Fe_2 - - - -
0881 Thalassiosira weissflogii CCMP1336 60nM Fe_2 - - - +
0898 Thalassiosira weissflogii CCMP1010 Replete - - - -
0899 Thalassiosira weissflogii CCMP1010 iron limitation - + + -
0900 Thalassiosira weissflogii CCMP1010 silica limitation - - - -
0901 Thalassiosira weissflogii CCMP1010 Nitrate Limitation - + + -
1405 Thalassiosira weissflogii CCMP1010 RepleteA - + - -
1406 Thalassiosira weissflogii CCMP1010 RepleteB - - - -
1407 Thalassiosira weissflogii CCMP1010 RepleteC - - - +
1408 Thalassiosira weissflogii CCMP1010 SiLimA - - - -
1409 Thalassiosira weissflogii CCMP1010 SiLimB - - - -
1410 Thalassiosira weissflogii CCMP1010 SiLimC - - - -
1411 Thalassiosira weissflogii CCMP1010 FeLimA - - - -
1412 Thalassiosira weissflogii CCMP1010 FeLimB - - - +
1413 Thalassiosira weissflogii CCMP1010 FeLimC - - - +
234546
Page 24
1414 Thalassiosira weissflogii CCMP1010 +2HrFeAddA - - - -
1415 Thalassiosira weissflogii CCMP1010 +2HrFeAddB - - + -
1416 Thalassiosira weissflogii CCMP1010 +2HrFeAddC - - - +
1417 Thalassiosira weissflogii CCMP1010 +8HrFeAddA - - - -
1418 Thalassiosira weissflogii CCMP1010 +8HrFeAddB - - - -
1419 Thalassiosira weissflogii CCMP1010 +8HrFeAddC - - - +
1420 Thalassiosira weissflogii CCMP1010 +24HrFeAddA - - - -
1421 Thalassiosira weissflogii CCMP1010 +24HrFeAddA - - - -
1422 Thalassiosira weissflogii CCMP1010 +24HrFeAddB - - + +
0152 Thalassiothrix antarctica L6-D1 CTR1 - - + +
0154 Thalassiothrix antarctica L6-D1 Fe1 - + + +
24
362
363
4748
Page 25
Supplementary table 3: list of sequences used in the phylogenetic analyses
Prostaglandin Enzyme Sequence ID Strain Specie Organism
COX1 MMETSP0707-20130614|6459 CCMP134 Asterionellopsis glacialis Microalga-Diatom
COX1 MMETSP1064-20121228|17645 CCAP1002/5 Aulacoseira subarctica Microalga-Diatom
COX1 MMETSP1064-20121228|57453 CCAP1002/5 Aulacoseira subarctica Microalga-Diatom
COX1 MMETSP0090-20130426|9955 CCMP159 Chaetoceros affinis Microalga-Diatom
COX1 MMETSP0717-20131115|51459 Chaetoceros curvisetus Microalga-Diatom
COX1 MMETSP0149-20130528|3320 MM31A-1 Chaetoceros debilis Microalga-Diatom
COX1 MMETSP0150-20130528|3844 MM31A-1 Chaetoceros debilis Microalga-Diatom
COX1 MMETSP0751-20121128|24195 CCMP1317 Chaetoceros neogracile Microalga-Diatom
COX1 MMETSP0751-20121128|3624 CCMP1317 Chaetoceros neogracile Microalga-Diatom
COX1 MMETSP1336-20130426|1470 RCC1993 Chaetoceros neogracile Microalga-Diatom
COX1 MMETSP1057-20121228|12622 CCMP338 Cyclotella meneghiniana Microalga-Diatom
COX1 MMETSP1057-20121228|4220 CCMP338 Cyclotella meneghiniana Microalga-Diatom
COX1 MMETSP1058-20130122|27510 CCMP353 Detonula confervacea Microalga-Diatom
COX1 MMETSP0907-20130614|13332 L2-C3 Fragilariopsis kerguelensis
Microalga-Diatom
COX1 MMETSP0009_2-20130614|5553 CCMP410 Grammatophora oceanica
Microalga-Diatom
COX1 MMETSP0014_2-20120614|6887 RCC80 Nitzschia sp Microalga-Diatom
COX1 MMETSP0015_2-20120614|17751 1302-5 Odontella aurita Microalga-Diatom
COX1 MMETSP0329-20121206|15499 B593 Pseudo-nitzschia arenysensis
Microalga-Diatom
COX1 MMETSP1061-20121228|74475 Pseudo-nitzschia pungens Microalga-Diatom
COX1 MMETSP0013_2-20120614|556 1716 Skeletonema costatum Microalga-Diatom
COX1 MMETSP0562-20121206|12376 SkelB Skeletonema dohrnii Microalga-Diatom
COX1 MMETSP0578-20130828|1168 CCMP1804 Skeletonema grethea Microalga-Diatom
COX1 MMETSP0319-20121206|1511 SM1012Hels-07 Skeletonema marinoi Microalga-Diatom
COX1 MMETSP0319-20121206|26582 SM1012Hels-07 Skeletonema marinoi Microalga-Diatom
COX1 MMETSP0320-20121206|19182 SM1012Den-03 Skeletonema marinoi Microalga-Diatom
COX1 MMETSP0920-20130426|33289 skelA Skeletonema marinoi Microalga-Diatom
COX1 MMETSP1039-20121108|4563 FE7 Skeletonema marinoi Microalga-Diatom
COX1 MMETSP1428-20130617|31509 UNC1201 Skeletonema marinoi Microalga-Diatom
COX1 MMETSP1428-20130617|783 UNC1201 Skeletonema marinoi Microalga-Diatom
COX1 MMETSP1361-20130828|23824 CCMP2646 Staurosira complex Microalga-Diatom
COX1 MMETSP1176-20130426|10672 CCMP1620 Synedropsis recta Microalga-Diatom
COX1 MMETSP0786-20121207|3776 CCMP1798 Thalassionema frauenfeldii
Microalga-Diatom
COX1 ADB65785 Caprella sp Animalia-shrimp
COXa XP_002127674 Ciona intestinalis Animalia-ascidia
25
364
4950
Page 26
COXb XP_002123273 Ciona intestinalis Animalia-ascidia
COX1 AFN20596 Coccotylus truncatus Macroalga-read alga
COX1 ADB65786 Gammarus sp Animalia-shrimp
COXa AAF93168 Gersemia fruticosa Animalia-soft coral
COXb AAS48061 Gersemia fruticosa Animalia-soft coral
COX1 NP000953 Homo sapiens Animalia-mammalia
COX2 NP000954 Homo sapiens Animalia-mammalia
COX1 WP015113127 Nostoc sp Bacteria-cyanobacteria
COX1 CAC10360.1 Oncorhynchus mykiss Animalia-rainbow trout
COX1 NP001009476 Ovis aries Animalia-mammalia
15S-specific-cyclooxygenase AAU87497 Plexaura homomalla Animalia-gorgonian
type coral
COX1 AAF93169 Plexaura homomalla Animalia-gorgonian type coral
PtgE synthase MMETSP1065-20121228_57710 CCMP125 Amphiprora paludosa Microalga-Diatom
PtgE synthase MMETSP0724-20121128_6497 CCMP467 Amphiprora sp Microalga-Diatom
PtgE synthase MMETSP0713-20130426_43092 Asterionellopsis glacialis Microalga-Diatom
PtgE synthase MMETSP1394-20130617_3078 CCMP1581 Asterionellopsis glacialis Microalga-Diatom
PtgE synthase MMETSP1449-20131203_4452 CCMP2084 Attheya septentrionalis Microalga-Diatom
PtgE synthase MMETSP1064-20121228_59152 CCAP1002/5 Aulacoseira subarctica Microalga-Diatom
PtgE synthase MMETSP0785-20121206_16150 CCMP1866 Bolidomonas pacifica Microalga-Diatom
PtgE synthase MMETSP0091-20130426_66594 CCMP159 Chaetoceros affinis Microalga-Diatom
PtgE synthase MMETSP0717-20131115_41249 Chaetoceros curvisetus Microalga-Diatom
PtgE synthase MMETSP0150-20130528_6846 MM31A-1 Chaetoceros debilis Microalga-Diatom
PtgE synthase MMETSP0150-20130528_6846 MM31A-1 Chaetoceros debilis Microalga-Diatom
PtgE synthase MMETSP1447-20131203_65193 CCMP1751 Chaetoceros dichaeta Microalga-Diatom
PtgE synthase MMETSP0753-20130528_8419 CCMP1317 Chaetoceros neogracile Microalga-Diatom
PtgE synthase MMETSP1336-20130426_5187 RCC1993 Chaetoceros neogracile Microalga-Diatom
PtgE synthase MMETSP1336-20130426_5187 RCC1993 Chaetoceros neogracile Microalga-Diatom
PtgE synthase MMETSP0200_2-20121128_3741 GSL56 Chaetoceros sp Microalga-Diatom
PtgE synthase MMETSP0010_2-20120614_2064 308 Corethron hystrix Microalga-Diatom
PtgE synthase MMETSP1066-20121228_203475 CCMP2513 Coscinodiscus wailesii Microalga-Diatom
PtgE synthase MMETSP1442-20131203_60396 CCMP3328 Craspedostauros australis
Microalga-Diatom
PtgE synthase MMETSP0397-20121206_3519 ECT3854 Cyclophora tenuis Microalga-Diatom
PtgE synthase MMETSP0017_2-20120614_4529 KMMCC:B-181 Cylindrotheca closterium Microalga-Diatom
PtgE synthase MMETSP0580-20130426_33201 Dactyliosolen fragilissimus
Microalga-Diatom
265152
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PtgE synthase MMETSP1058-20130122_59290 CCMP353 Detonula confervacea Microalga-Diatom
PtgE synthase MMETSP1443-20131203_66539 CCMP2396 Entomoneis sp Microalga-Diatom
PtgE synthase MMETSP0735-20130129_6806 L26-C5 Fragilariopsis kerguelensis
Microalga-Diatom
PtgE synthase MMETSP1360-20130828_7305 CCMP2313 Licmophora paradoxa Microalga-Diatom
PtgE synthase MMETSP1322-20130828_5091 RCC2270 Minutocellus polymorphus
Microalga-Diatom
PtgE synthase MMETSP0014_2-20120614_25505 RCC80 Nitzschia sp Microalga-Diatom
PtgE synthase MMETSP0014_2-20120614_43557 RCC80 Nitzschia sp Microalga-Diatom
PtgE synthase MMETSP0015_2-20120614_4973 isolate 1302-5 Odontella aurita Microalga-Diatom
PtgE synthase MMETSP0160_2-20130417_17287 Grunow 1884 Odontella sinensis Microalga-Diatom
PtgE synthase MMETSP0160_2-20130417_2021 Grunow 1884 Odontella sinensis Microalga-Diatom
PtgE synthase MMETSP0176-20130528_3501 PI-D3 Proboscia alata Microalga-Diatom
PtgE synthase MMETSP0816-20121228_18404 CCAP1064/1 Proboscia inermis Microalga-Diatom
PtgE synthase MMETSP0329-20121206_22727 B593 Pseudo-nitzschia arenysensis
Microalga-Diatom
PtgE synthase MMETSP0327-20121206_12465 B596 Pseudo-nitzschia delicatissima
Microalga-Diatom
PtgE synthase MMETSP0852-20130820_66215 WWA7 Pseudo-nitzschia fraudulenta
Microalga-Diatom
PtgE synthase MMETSP1423-20130617_783 UNC1101 Pseudo-nitzschia heimii Microalga-Diatom
PtgE synthase comp15013_c0 Small size female
Pseudo-nitzschia multistriata
Microalga-Diatom
PtgE synthase MMETSP1060-20121228_69939 Pseudo-nitzschia pungens Microalga-Diatom
PtgE synthase MMETSP0141_2-20121125_8366 10249 10 AB Pseudo-nitzschia_australis
Microalga-Diatom
PtgE synthase MMETSP0789-20121207_286 CCMP1694 Rhizosolenia setigera Microalga-Diatom
PtgE synthase MMETSP1039-20121108_5744 FE7 Skeletonema marinoi Microalga-Diatom
PtgE synthase MMETSP0794_2-20130614_15797 CCMP815 Stephanopyxis turris Microalga-Diatom
PtgE synthase MMETSP0786-20121207_2746 CCMP1798 Thalassionema frauenfeldii
Microalga-Diatom
PtgE synthase MMETSP0156-20130528_14046 L26-B Thalassionema nitzschioides
Microalga-Diatom
PtgE synthase MMETSP0905-20130828_2730 CCMP982 Thalassiosira antarctica Microalga-Diatom
PtgE synthase MMETSP0739-20130205_36184 CCMP1093 Thalassiosira miniscula Microalga-Diatom
PtgE synthase MMETSP0970-20121206_26001 CCMP1005 Thalassiosira oceanica Microalga-Diatom
PtgE synthase MMETSP0971-20121206_7568 CCMP1005 Thalassiosira oceanica Microalga-Diatom
PtgE synthase MMETSP0403-20120918_4534 CCMP3096 Thalassiosira rotula Microalga-Diatom
PtgE synthase MMETSP1059-20121228_69676 FW Thalassiosira sp Microalga-Diatom
PtgE synthase MMETSP1071-20121207_12737 NH16 Thalassiosira sp Microalga-Diatom
PtgE synthase MMETSP0899_2-20130528_50683 CCMP1010 Thalassiosira weissflogii Microalga-Diatom
PtgE synthase MMETSP0901_2-20130828_2659 CCMP1010 Thalassiosira weissflogii Microalga-Diatom
PtgE synthase MMETSP1405-20130829_715 CCMP1010 Thalassiosira weissflogii Microalga-Diatom
PtgE synthase MMETSP0154-20130528_3647 L6-D1 Thalassiothrix antarctica Microalga-Diatom
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PtgE synthase2 XP_006846913 Amborella trichopoda Plant
PtgE synthase2 OAY77991 Ananas comosus Plant
PtgE synthase2 like XP_015933989 Arachis duranensis Plant
PtgE synthase2 KYP65502 Cajanus cajan Plant
PtgE synthase2 like XP_010496074 Camelina sativa Plant
PtgE synthase2 like XP_004512741 Cicer arietinum Plant
PtgE synthase2 like XP_008453485 Cucumis melo Plant
PtgE synthase2 like XP_004137346 Cucumis sativus Plant
Electron carrier/proteindisulfide
oxidoreductaseXP_005785506 Emiliania huxleyi Microalga-
coccolithophore
PtgE synthase2 like XP_010069798 Eucalyptus grandis Plant
PtgE synthase2 KHN44626 Glycine soja Plant
PtgE synthase2 XP_012447492 Gossypium raimondii Plant
PtgE synthase2 EFN78959 Harpegnathos saltator Animalia-insect
PtgE synthase2 like XP_002161497 Hydra vulgaris Animalia-polyp
Glutathione_S-transferase XP_002406052 Ixodes scapularis Animalia-arthropod
PtgE synthase2 like XP_008393428 Malus domestica Plant
Glutathione_S-transferase XP_003619823 Medicago truncatula Plant
PtgE synthase2 XP_009400344 Musa acuminata Plant
Glutaredoxin EWM25410 Nannochloropsis gaditana Microalga
PtgE synthase2 like XP_010273922 Nelumbo nucifera Plant
PtgE synthase2 XP_009772831 Nicotiana sylvestris Plant
PtgE synthase2 CAH67930 Oryza sativa Plant
PtgE synthase2 like XP_015924269 Parasteatoda tepidariorum
Animalia-arthropode
PtgE synthase2 XP_011003060 Populus euphratica Plant
PtgE synthase2 like XP_007209338 Prunus persica Plant
PtgE synthase2 like XP_009356033 Pyrus bretschneideri Plant
Glutathione-S-transferase/glutaredoxin XP_845973 Trypanosoma brucei Excavata-protozoa
PtgE synthase2 XP_002282513 Vitis vinifera Plant
PtgE synthase2 XP_015882743 Ziziphus jujuba Plant
PtgE synthase KMZ69256 Zostera marina Plant
PtgH isomerase MMETSP1065-20121228_10087 CCMP125 Amphiprora paludosa Microalga-Diatom
PtgH isomerase MMETSP1065-20121228_1088 CCMP125 Amphiprora paludosa Microalga-Diatom
PtgH isomerase MMETSP1065-20121228_67778 CCMP125 Amphiprora paludosa Microalga-Diatom
PtgH isomerase MMETSP0725-20121227_3824 CCMP467 Amphiprora sp Microalga-Diatom
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PtgH isomerase MMETSP0724-20121128_16256 CCMP467 Amphiprora sp. Microalga-Diatom
PtgH isomerase MMETSP0724-20121128_16367 CCMP467 Amphiprora sp. Microalga-Diatom
PtgH isomerase MMETSP0725-20121227_10193 CCMP467 Amphiprora sp. Microalga-Diatom
PtgH isomerase MMETSP0318_2-20130129_1568 CCMP127 Amphora coffeaeformis Microalga-Diatom
PtgH isomerase MMETSP0318_2-20130129_6994 CCMP127 Amphora coffeaeformis Microalga-Diatom
PtgH isomerase MMETSP0706-20130129_10541 CCMP134 Asterionellopsis glacialis Microalga-Diatom
PtgH isomerase MMETSP0706-20130129_10593 CCMP134 Asterionellopsis glacialis Microalga-Diatom
PtgH isomerase MMETSP0706-20130129_13708 CCMP134 Asterionellopsis glacialis Microalga-Diatom
PtgH isomerase MMETSP0707-20130614_13155 CCMP134 Asterionellopsis glacialis Microalga-Diatom
PtgH isomerase MMETSP0707-20130614_214 CCMP134 Asterionellopsis glacialis Microalga-Diatom
PtgH isomerase MMETSP0707-20130614_37117 CCMP134 Asterionellopsis glacialis Microalga-Diatom
PtgH isomerase MMETSP0713-20130426_21585 Asterionellopsis glacialis Microalga-Diatom
PtgH isomerase MMETSP0713-20130426_4592 Asterionellopsis glacialis Microalga-Diatom
PtgH isomerase MMETSP1394-20130617_12516 CCMP1581 Asterionellopsis glacialis Microalga-Diatom
PtgH isomerase MMETSP1394-20130617_1597 CCMP1581 Asterionellopsis glacialis Microalga-Diatom
PtgH isomerase MMETSP1394-20130617_4507 CCMP1581 Asterionellopsis glacialis Microalga-Diatom
PtgH isomerase MMETSP1394-20130617_879 CCMP1581 Asterionellopsis glacialis Microalga-Diatom
PtgH isomerase MMETSP0418-20121206_7922 13vi08-1A Astrosyne radiata Microalga-Diatom
PtgH isomerase MMETSP1449-20131203_1075 CCMP2084 Attheya septentrionalis Microalga-Diatom
PtgH isomerase MMETSP1449-20131203_30661 CCMP2084 Attheya septentrionalis Microalga-Diatom
PtgH isomerase MMETSP1449-20131203_37398 CCMP2084 Attheya septentrionalis Microalga-Diatom
PtgH isomerase MMETSP1449-20131203_52238 CCMP2084 Attheya septentrionalis Microalga-Diatom
PtgH isomerase MMETSP1064-20121228_1534 CCAP1002/5 Aulacoseira subarctica Microalga-Diatom
PtgH isomerase MMETSP1064-20121228_17710 CCAP1002/5 Aulacoseira subarctica Microalga-Diatom
PtgH isomerase MMETSP0785-20121206_15367 CCMP1866 Bolidomonas pacifica Microalga-Diatom
PtgH isomerase MMETSP0785-20121206_4282 CCMP1866 Bolidomonas pacifica Microalga-Diatom
PtgH isomerase MMETSP0088-20131115_39681 CCMP159 Chaetoceros affinis Microalga-Diatom
PtgH isomerase MMETSP0090-20130426_5481 CCMP159 Chaetoceros affinis Microalga-Diatom
PtgH isomerase MMETSP0091-20130426_1954 CCMP159 Chaetoceros affinis Microalga-Diatom
PtgH isomerase MMETSP0092-20131115_14717 CCMP159 Chaetoceros affinis Microalga-Diatom
PtgH isomerase MMETSP0717-20131115_17446 Chaetoceros curvisetus Microalga-Diatom
PtgH isomerase MMETSP0717-20131115_2320 Chaetoceros curvisetus Microalga-Diatom
PtgH isomerase MMETSP0718-20130828_4404 Chaetoceros curvisetus Microalga-Diatom
PtgH isomerase MMETSP0149-20130528_3694 MM31A-1 Chaetoceros debilis Microalga-Diatom
PtgH isomerase MMETSP0149-20130528_42183 MM31A-1 Chaetoceros debilis Microalga-Diatom
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PtgH isomerase MMETSP0150-20130528_11308 MM31A-1 Chaetoceros debilis Microalga-Diatom
PtgH isomerase MMETSP0150-20130528_47516 MM31A-1 Chaetoceros debilis Microalga-Diatom
PtgH isomerase MMETSP1447-20131203_4865 CCMP1751 Chaetoceros dichaeta Microalga-Diatom
PtgH isomerase MMETSP1447-20131203_7059 CCMP1751 Chaetoceros dichaeta Microalga-Diatom
PtgH isomerase MMETSP0751-20121128_12958 CCMP1317 Chaetoceros neogracile Microalga-Diatom
PtgH isomerase MMETSP0752-20121128_17504 CCMP1317 Chaetoceros neogracile Microalga-Diatom
PtgH isomerase MMETSP0754-20121128_24063 CCMP1317 Chaetoceros neogracile Microalga-Diatom
PtgH isomerase MMETSP1336-20130426_27716 RCC1993 Chaetoceros neogracile Microalga-Diatom
PtgH isomerase MMETSP1336-20130426_3079 RCC1993 Chaetoceros neogracile Microalga-Diatom
PtgH isomerase MMETSP1336-20130426_8885 RCC1993 Chaetoceros neogracile Microalga-Diatom
PtgH isomerase MMETSP1336-20130426_949 RCC1993 Chaetoceros neogracile Microalga-Diatom
PtgH isomerase MMETSP0200_2-20121128_14879 GSL56 Chaetoceros sp. Microalga-Diatom
PtgH isomerase MMETSP0200_2-20121128_2287 GSL56 Chaetoceros sp. Microalga-Diatom
PtgH isomerase MMETSP1429-20130617_66082 UNC1202 Chaetoceros sp. Microalga-Diatom
PtgH isomerase MMETSP1429-20130617_7353 UNC1202 Chaetoceros sp. Microalga-Diatom
PtgH isomerase MMETSP1429-20130617_83425 UNC1202 Chaetoceros sp. Microalga-Diatom
PtgH isomerase MMETSP0010_2-20120614_11223 308 Corethron hystrix Microalga-Diatom
PtgH isomerase MMETSP0010_2-20120614_7429 308 Corethron hystrix Microalga-Diatom
PtgH isomerase MMETSP0010_2-20120614_9630 308 Corethron hystrix Microalga-Diatom
PtgH isomerase MMETSP0169-20130528_22966 L29A3 Corethron pennatum Microalga-Diatom
PtgH isomerase MMETSP0169-20130528_37520 L29A3 Corethron pennatum Microalga-Diatom
PtgH isomerase MMETSP0169-20130528_3970 L29A3 Corethron pennatum Microalga-Diatom
PtgH isomerase MMETSP0171-20130528_2082 L29A3 Corethron pennatum Microalga-Diatom
PtgH isomerase MMETSP0171-20130528_22217 L29A3 Corethron pennatum Microalga-Diatom
PtgH isomerase MMETSP0171-20130528_4142 L29A3 Corethron pennatum Microalga-Diatom
PtgH isomerase MMETSP0171-20130528_55019 L29A3 Corethron pennatum Microalga-Diatom
PtgH isomerase MMETSP0171-20130528_56053 L29A3 Corethron pennatum Microalga-Diatom
PtgH isomerase MMETSP0171-20130528_57107 L29A3 Corethron pennatum Microalga-Diatom
PtgH isomerase MMETSP0171-20130528_94319 L29A3 Corethron pennatum Microalga-Diatom
PtgH isomerase MMETSP0171-20130528_98897 L29A3 Corethron pennatum Microalga-Diatom
PtgH isomerase MMETSP1066-20121228_16401 CCMP2513 Coscinodiscus wailesii Microalga-Diatom
PtgH isomerase MMETSP1066-20121228_260596 CCMP2513 Coscinodiscus wailesii Microalga-Diatom
PtgH isomerase MMETSP1066-20121228_84979 CCMP2513 Coscinodiscus wailesii Microalga-Diatom
PtgH isomerase MMETSP1442-20131203_40613 CCMP3328 Craspedostauros australis
Microalga-Diatom
PtgH isomerase MMETSP1442-20131203_4715 CCMP3328 Craspedostauros australis
Microalga-Diatom
PtgH isomerase MMETSP1442-20131203_5597 CCMP3328 Craspedostauros Microalga-Diatom
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australis
PtgH isomerase MMETSP1442-20131203_59506 CCMP3328 Craspedostauros australis
Microalga-Diatom
PtgH isomerase MMETSP0397-20121206_19965 ECT3854 Cyclophora tenuis Microalga-Diatom
PtgH isomerase MMETSP0397-20121206_2507 ECT3854 Cyclophora tenuis Microalga-Diatom
PtgH isomerase MMETSP0397-20121206_3814 ECT3854 Cyclophora tenuis Microalga-Diatom
PtgH isomerase MMETSP1057-20121228_314 CCMP 338 Cyclotella meneghiniana Microalga-Diatom
PtgH isomerase MMETSP0017_2-20120614_18451 KMMCC:B-181 Cylindrotheca closterium Microalga-Diatom
PtgH isomerase MMETSP0017_2-20120614_27112 KMMCC:B-181 Cylindrotheca closterium Microalga-Diatom
PtgH isomerase MMETSP0017_2-20120614_2763 KMMCC:B-181 Cylindrotheca closterium Microalga-Diatom
PtgH isomerase MMETSP0017_2-20120614_376 KMMCC:B-181 Cylindrotheca closterium Microalga-Diatom
PtgH isomerase MMETSP0580-20130426_8153 Dactyliosolen fragilissimus
Microalga-Diatom
PtgH isomerase MMETSP0580-20130426_9785 Dactyliosolen fragilissimus
Microalga-Diatom
PtgH isomerase MMETSP1058-20130122_15807 CCMP 353 Detonula confervacea Microalga-Diatom
PtgH isomerase MMETSP1058-20130122_19850 CCMP 353 Detonula confervacea Microalga-Diatom
PtgH isomerase MMETSP1058-20130122_7945 CCMP 353 Detonula confervacea Microalga-Diatom
PtgH isomerase MMETSP1443-20131203_181 CCMP2396 Entomoneis sp. Microalga-Diatom
PtgH isomerase MMETSP1443-20131203_308 CCMP2396 Entomoneis sp. Microalga-Diatom
PtgH isomerase MMETSP1443-20131203_68585 CCMP2396 Entomoneis sp. Microalga-Diatom
PtgH isomerase MMETSP1437-20131217_32895 CCMP1452 Eucampia antarctica Microalga-Diatom
PtgH isomerase MMETSP0733-20121128_4596 L26-C5 Fragilariopsis kerguelensis
Microalga-Diatom
PtgH isomerase MMETSP0733-20121128_6486 L26-C5 Fragilariopsis kerguelensis
Microalga-Diatom
PtgH isomerase MMETSP0734-20130129_9994 L26-C5 Fragilariopsis kerguelensis
Microalga-Diatom
PtgH isomerase MMETSP0735-20130129_21087 L26-C5 Fragilariopsis kerguelensis
Microalga-Diatom
PtgH isomerase MMETSP0735-20130129_30216 L26-C5 Fragilariopsis kerguelensis
Microalga-Diatom
PtgH isomerase MMETSP0736-20130129_19763 L26-C5 Fragilariopsis kerguelensis
Microalga-Diatom
PtgH isomerase MMETSP0908-20130614_11536 L2-C3 Fragilariopsis kerguelensis
Microalga-Diatom
PtgH isomerase MMETSP0908-20130614_5886 L2-C3 Fragilariopsis kerguelensis
Microalga-Diatom
PtgH isomerase MMETSP0009_2-20130614_10662 CCMP 410 Grammatophora oceanica
Microalga-Diatom
PtgH isomerase MMETSP0009_2-20130614_6488 CCMP 410 Grammatophora oceanica
Microalga-Diatom
PtgH isomerase MMETSP0009_2-20130614_81747 CCMP 410 Grammatophora oceanica
Microalga-Diatom
PtgH isomerase MMETSP0322-20121206_14221 B651 Leptocylindrus apora Microalga-Diatom
PtgH isomerase MMETSP0322-20121206_7581 B651 Leptocylindrus apora Microalga-Diatom
PtgH isomerase MMETSP1362-20130617_1296 CCMP1856 Leptocylindrus danicus Microalga-Diatom
PtgH isomerase MMETSP1362-20130617_29537 CCMP1856 Leptocylindrus danicus Microalga-Diatom
PtgH isomerase MMETSP1362-20130617_84541 CCMP1856 Leptocylindrus danicus Microalga-Diatom
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PtgH isomerase MMETSP1360-20130828_11548 CCMP2313 Licmophora paradoxa Microalga-Diatom
PtgH isomerase MMETSP1360-20130828_3247 CCMP2313 Licmophora paradoxa Microalga-Diatom
PtgH isomerase MMETSP1070-20121207_3283 NH13 Minutocellulus polymorphus
Microalga-Diatom
PtgH isomerase MMETSP1070-20121207_7688 NH13 Minutocellulus polymorphus
Microalga-Diatom
PtgH isomerase MMETSP1322-20130828_31186 RCC2270 Minutocellulus polymorphus
Microalga-Diatom
PtgH isomerase MMETSP1322-20130828_3156 RCC2270 Minutocellulus polymorphus
Microalga-Diatom
PtgH isomerase MMETSP0744-20121128_10137 CCMP561 Nitzschia punctata Microalga-Diatom
PtgH isomerase MMETSP0747-20121128_14861 CCMP561 Nitzschia punctata Microalga-Diatom
PtgH isomerase MMETSP0014_2-20120614_15951 RCC80 Nitzschia sp Microalga-Diatom
PtgH isomerase MMETSP0014_2-20120614_27091 RCC80 Nitzschia sp Microalga-Diatom
PtgH isomerase MMETSP0014_2-20120614_6366 RCC80 Nitzschia sp Microalga-Diatom
PtgH isomerase MMETSP0015_2-20120614_28361 isolate 1302-5 Odontella aurita Microalga-Diatom
PtgH isomerase MMETSP0015_2-20120614_3716 isolate 1302-5 Odontella aurita Microalga-Diatom
PtgH isomerase MMETSP0015_2-20120614_3957 isolate 1302-5 Odontella aurita Microalga-Diatom
PtgH isomerase MMETSP0160_2-20130417_1657 Grunow 1884 Odontella sinensis Microalga-Diatom
PtgH isomerase MMETSP0160_2-20130417_3338 Grunow 1884 Odontella sinensis Microalga-Diatom
PtgH isomerase MMETSP0160_2-20130417_3948 Grunow 1884 Odontella sinensis Microalga-Diatom
PtgH isomerase MMETSP0160_2-20130417_54523 Grunow 1884 Odontella sinensis Microalga-Diatom
PtgH isomerase MMETSP0160_2-20130417_667 Grunow 1884 Odontella sinensis Microalga-Diatom
PtgH isomerase MMETSP0160_2-20130417_8721 Grunow 1884 Odontella sinensis Microalga-Diatom
PtgH isomerase MMETSP0174-20130528_18662 PI-D3 Proboscia alata Microalga-Diatom
PtgH isomerase MMETSP0174-20130528_22148 PI-D3 Proboscia alata Microalga-Diatom
PtgH isomerase MMETSP0176-20130528_13901 PI-D3 Proboscia alata Microalga-Diatom
PtgH isomerase MMETSP0176-20130528_2071 PI-D3 Proboscia alata Microalga-Diatom
PtgH isomerase MMETSP0176-20130528_24740 PI-D3 Proboscia alata Microalga-Diatom
PtgH isomerase MMETSP0176-20130528_6534 PI-D3 Proboscia alata Microalga-Diatom
PtgH isomerase MMETSP0816-20121228_114861 CCAP1064/1 Proboscia inermis Microalga-Diatom
PtgH isomerase MMETSP0816-20121228_115114 CCAP1064/1 Proboscia inermis Microalga-Diatom
PtgH isomerase MMETSP0816-20121228_20559 CCAP1064/1 Proboscia inermis Microalga-Diatom
PtgH isomerase MMETSP0816-20121228_22070 CCAP1064/1 Proboscia inermis Microalga-Diatom
PtgH isomerase MMETSP0329-20121206_12053 B593 Pseudo-nitzschia arenysensis
Microalga-Diatom
PtgH isomerase MMETSP0329-20121206_1757 B593 Pseudo-nitzschia arenysensis
Microalga-Diatom
PtgH isomerase MMETSP0329-20121206_6712 B593 Pseudo-nitzschia arenysensis
Microalga-Diatom
PtgH isomerase MMETSP0140_2-20121125_19224 10249 10 AB Pseudo-nitzschia australis
Microalga-Diatom
PtgH isomerase MMETSP0141_2-20121125_8528 10249 10 AB Pseudo-nitzschia australis
Microalga-Diatom
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PtgH isomerase MMETSP0142_2-20121227_13165 10249 10 AB Pseudo-nitzschia australis
Microalga-Diatom
PtgH isomerase MMETSP0327-20121206_4279 B596 Pseudo-nitzschia delicatissima
Microalga-Diatom
PtgH isomerase MMETSP0327-20121206_8227 B596 Pseudo-nitzschia delicatissima
Microalga-Diatom
PtgH isomerase MMETSP0851-20130426_113318 WWA7 Pseudo-nitzschia fraudulenta
Microalga-Diatom
PtgH isomerase MMETSP0851-20130426_11958 WWA7 Pseudo-nitzschia fraudulenta
Microalga-Diatom
PtgH isomerase MMETSP0851-20130426_154403 WWA7 Pseudo-nitzschia fraudulenta
Microalga-Diatom
PtgH isomerase MMETSP0851-20130426_65473 WWA7 Pseudo-nitzschia fraudulenta
Microalga-Diatom
PtgH isomerase MMETSP0851-20130426_71847 WWA7 Pseudo-nitzschia fraudulenta
Microalga-Diatom
PtgH isomerase MMETSP0851-20130426_86685 WWA7 Pseudo-nitzschia fraudulenta
Microalga-Diatom
PtgH isomerase MMETSP0852-20130820_3503 WWA7 Pseudo-nitzschia fraudulenta
Microalga-Diatom
PtgH isomerase MMETSP1423-20130617_153 UNC1101 Pseudo-nitzschia heimii Microalga-Diatom
PtgH isomerase MMETSP1423-20130617_4377 UNC1101 Pseudo-nitzschia heimii Microalga-Diatom
PtgH isomerase MMETSP1423-20130617_7437 UNC1101 Pseudo-nitzschia heimii Microalga-Diatom
PtgH isomerase comp10947_c0_seq1 Female small size
Pseudo-nitzschia multistriata
Microalga-Diatom
PtgH isomerase comp10987_c0_seq1 Female small size
Pseudo-nitzschia multistriata
Microalga-Diatom
PtgH isomerase comp2865_c0_seq1 Female small size
Pseudo-nitzschia multistriata
Microalga-Diatom
PtgH isomerase MMETSP1061-20121228_2541 Pseudo-nitzschia pungens Microalga-Diatom
PtgH isomerase MMETSP1061-20121228_50332 Pseudo-nitzschia pungens Microalga-Diatom
PtgH isomerase MMETSP1061-20121228_65282 Pseudo-nitzschia pungens Microalga-Diatom
PtgH isomerase MMETSP0789-20121207_12219 CCMP1694 Rhizosolenia setigera Microalga-Diatom
PtgH isomerase MMETSP0789-20121207_22217 CCMP1694 Rhizosolenia setigera Microalga-Diatom
PtgH isomerase MMETSP0789-20121207_6008 CCMP1694 Rhizosolenia setigera Microalga-Diatom
PtgH isomerase MMETSP0013_2-20120614_1402 1716 Skeletonema costatum Microalga-Diatom
PtgH isomerase MMETSP0013_2-20120614_7554 1716 Skeletonema costatum Microalga-Diatom
PtgH isomerase MMETSP0578-20130828_3923 CCMP1804 Skeletonema grethae Microalga-Diatom
PtgH isomerase MMETSP0593-20130828_9896 CCMP2506 Skeletonema japonicum Microalga-Diatom
PtgH isomerase MMETSP0320-20121206_14352 SM1012Den-03 Skeletonema marinoi Microalga-Diatom
PtgH isomerase MMETSP1039-20121108_1431 FE7 Skeletonema marinoi Microalga-Diatom
PtgH isomerase MMETSP0603-20130417_1199 CCMP793 Skeletonema menzelii Microalga-Diatom
PtgH isomerase MMETSP0603-20130417_164 CCMP793 Skeletonema menzelii Microalga-Diatom
PtgH isomerase MMETSP0794_2-20130614_10777 CCMP815 Stephanopyxis turris Microalga-Diatom
PtgH isomerase MMETSP0794_2-20130614_13802 CCMP815 Stephanopyxis turris Microalga-Diatom
PtgH isomerase MMETSP0794_2-20130614_13810 CCMP815 Stephanopyxis turris Microalga-Diatom
PtgH isomerase MMETSP0800-20121206_5993 CCMP2910 Striatella unipunctata Microalga-Diatom
PtgH isomerase MMETSP1176-20130426_1126 CCMP1620 Synedropsis recta Microalga-Diatom
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PtgH isomerase MMETSP1176-20130426_2088 CCMP1620 Synedropsis recta Microalga-Diatom
PtgH isomerase MMETSP1176-20130426_2727 CCMP1620 Synedropsis recta Microalga-Diatom
PtgH isomerase MMETSP1176-20130426_4557 CCMP1620 Synedropsis recta Microalga-Diatom
PtgH isomerase MMETSP1176-20130426_7053 CCMP1620 Synedropsis recta Microalga-Diatom
PtgH isomerase MMETSP1176-20130426_9434 CCMP1620 Synedropsis recta Microalga-Diatom
PtgH isomerase MMETSP0786-20121207_16667 CCMP1798 Thalassionema frauenfeldii
Microalga-Diatom
PtgH isomerase MMETSP0786-20121207_18202 CCMP1798 Thalassionema frauenfeldii
Microalga-Diatom
PtgH isomerase MMETSP0786-20121207_4968 CCMP1798 Thalassionema frauenfeldii
Microalga-Diatom
PtgH isomerase MMETSP0786-20121207_5691 CCMP1798 Thalassionema frauenfeldii
Microalga-Diatom
PtgH isomerase MMETSP0156-20130528_11107 L26-B Thalassionema nitzschioides
Microalga-Diatom
PtgH isomerase MMETSP0156-20130528_1359 L26-B Thalassionema nitzschioides
Microalga-Diatom
PtgH isomerase MMETSP0158-20130606_17074 L26-B Thalassionema nitzschioides
Microalga-Diatom
PtgH isomerase MMETSP0158-20130606_6010 L26-B Thalassionema nitzschioides
Microalga-Diatom
PtgH isomerase MMETSP0902-20130614_41792 CCMP982 Thalassiosira antarctica Microalga-Diatom
PtgH isomerase MMETSP0903-20130614_658 CCMP982 Thalassiosira antarctica Microalga-Diatom
PtgH isomerase MMETSP0904-20121228_17593 CCMP982 Thalassiosira antarctica Microalga-Diatom
PtgH isomerase MMETSP0492-20130828_47214 GMp14c1 Thalassiosira gravida Microalga-Diatom
PtgH isomerase MMETSP0737-20130205_6022 CCMP1093 Thalassiosira miniscula Microalga-Diatom
PtgH isomerase MMETSP0739-20130205_39664 CCMP1093 Thalassiosira miniscula Microalga-Diatom
PtgH isomerase MMETSP0970-20121206_1919 CCMP1005 Thalassiosira oceanica Microalga-Diatom
PtgH isomerase MMETSP0970-20121206_25055 CCMP1005 Thalassiosira oceanica Microalga-Diatom
PtgH isomerase MMETSP1067-20121228_17924 Tpunct2005C2 Thalassiosira puntigera Microalga-Diatom
PtgH isomerase MMETSP1067-20121228_6006 Tpunct2005C2 Thalassiosira puntigera Microalga-Diatom
PtgH isomerase MMETSP0404_2-20130426_37981 CCMP3096 Thalassiosira rotula Microalga-Diatom
PtgH isomerase MMETSP0910-20130528_8791 GSO102 Thalassiosira rotula Microalga-Diatom
PtgH isomerase MMETSP1059-20121228_75945 FW Thalassiosira sp Microalga-Diatom
PtgH isomerase MMETSP1071-20121207_10010 NH16 Thalassiosira sp Microalga-Diatom
PtgH isomerase MMETSP1071-20121207_6566 NH16 Thalassiosira sp Microalga-Diatom
PtgH isomerase MMETSP0899_2-20130528_8000 CCMP1010 Thalassiosira weissflogii Microalga-Diatom
PtgH isomerase MMETSP0901_2-20130828_34040 CCMP1010 Thalassiosira weissflogii Microalga-Diatom
PtgH isomerase MMETSP1415-20130829_7831 CCMP1010 Thalassiosira weissflogii Microalga-Diatom
PtgH isomerase MMETSP1422-20130829_36592 CCMP1010 Thalassiosira weissflogii Microalga-Diatom
PtgH isomerase MMETSP0152-20130528_2239 L6-D1 Thalassiothrix antarctica Microalga-Diatom
PtgH isomerase MMETSP0154-20130528_10967 L6-D1 Thalassiothrix antarctica Microalga-Diatom
PtgH isomerase MMETSP0154-20130528_21435 L6-D1 Thalassiothrix antarctica Microalga-Diatom
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PtgH isomerase MMETSP0154-20130528_3046 L6-D1 Thalassiothrix antarctica Microalga-Diatom
PtgH isomerase MMETSP0154-20130528_45727 L6-D1 Thalassiothrix antarctica Microalga-Diatom
PtgH isomerase MMETSP0154-20130528_59947 L6-D1 Thalassiothrix antarctica Microalga-Diatom
PtgH isomerase MMETSP0154-20130528_9378 L6-D1 Thalassiothrix antarctica Microalga-Diatom
Glutathione S-transferase B1N8I3 _CHOCR Chondrus crispus Macroalga-red alga
Glutathione s-transferase-like protein KOO22099 CCMP291 Chrysochromulina sp. Microalga
Glutathione-requiring prostaglandin D synthase EKC38075 Crassostrea gigas Animalia-oyster
Glutathione S-transferase CBJ30560 Ectocarpus siliculosus Macroalga-brown alga
Glutathione S-transferase ABR09274 Laminaria digitata Macroalga-brown alga
PtgH isomerase EGZ23680 Phytophthora sojae Plant
Glutathione S-transferase WP006973551 Plesiocystis pacifica Bacteria-marine micro-bacterium
Glutathione S-transferase ACV31082 Pyropia yezoensis Macroalga-red alga
PtgH isomerase XP004998907 Salpingoeca rosetta Microeukaryotes-choanoflagellate
Glutathione S-transferase-like protein AFV00540 Simiduia agarivorans Bacteria-marine
bacterium
ProstaglandinD synthase XP014151970 Sphaeroforma arctica Bacteria-marine bacterium
Glutathione S-transferase WP004371111 Thauera phenylacetica Bacteria
Ptg transporter MMETSP1065-20121228_11996 CCMP125 Amphiprora paludosa Microalga-Diatom
Ptg transporter MMETSP1065-20121228_67738 CCMP125 Amphiprora paludosa Microalga-Diatom
Ptg transporter MMETSP0724-20121128_19505 CCMP467 Amphiprora sp Microalga-Diatom
Ptg transporter MMETSP0724-20121128_3834 CCMP467 Amphiprora sp Microalga-Diatom
Ptg transporter MMETSP0725-20121227_2351 CCMP467 Amphiprora sp Microalga-Diatom
Ptg transporter MMETSP0725-20121227_380 CCMP467 Amphiprora sp Microalga-Diatom
Ptg transporter MMETSP0727-20121128_11428 CCMP467 Amphiprora sp Microalga-Diatom
Ptg transporter MMETSP0727-20121128_14199 CCMP467 Amphiprora sp Microalga-Diatom
Ptg transporter MMETSP0727-20121128_8152 CCMP467 Amphiprora sp Microalga-Diatom
Ptg transporter MMETSP0316-20121206_6107 CCMP127 Amphora coffeaeformis Microalga-Diatom
Ptg transporter MMETSP0707-20130614_37570 CCMP134 Asterionellopsis glacialis Microalga-Diatom
Ptg transporter MMETSP0707-20130614_38189 CCMP134 Asterionellopsis glacialis Microalga-Diatom
Ptg transporter MMETSP0707-20130614_38286 CCMP134 Asterionellopsis glacialis Microalga-Diatom
Ptg transporter MMETSP0707-20130614_38952 CCMP134 Asterionellopsis glacialis Microalga-Diatom
Ptg transporter MMETSP1394-20130617_26855 CCMP1581 Asterionellopsis glacialis Microalga-Diatom
Ptg transporter MMETSP1394-20130617_29306 CCMP1581 Asterionellopsis glacialis Microalga-Diatom
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Ptg transporter MMETSP1394-20130617_3401 CCMP1581 Asterionellopsis glacialis Microalga-Diatom
Ptg transporter MMETSP0418-20121206_1955 13vi08-1A Astrosyne radiata Microalga-Diatom
Ptg transporter MMETSP1449-20131203_57789 CCMP2084 Attheya septentrionalis Microalga-Diatom
Ptg transporter MMETSP1064-20121228_70784 CCAP 1002/5 Aulacoseira subarctica Microalga-Diatom
Ptg transporter MMETSP1064-20121228_9085 CCAP 1002/5 Aulacoseira subarctica Microalga-Diatom
Ptg transporter MMETSP0090-20130426_47634 CCMP159 Chaetoceros affinis Microalga-Diatom
Ptg transporter MMETSP0090-20130426_48899 CCMP159 Chaetoceros affinis Microalga-Diatom
Ptg transporter MMETSP0090-20130426_48899 CCMP159 Chaetoceros affinis Microalga-Diatom
Ptg transporter MMETSP0090-20130426_49119 CCMP159 Chaetoceros affinis Microalga-Diatom
Ptg transporter MMETSP0091-20130426_70237 CCMP159 Chaetoceros affinis Microalga-Diatom
Ptg transporter MMETSP0091-20130426_70237 CCMP159 Chaetoceros affinis Microalga-Diatom
Ptg transporter MMETSP0717-20131115_847 Chaetoceros curvisetus Microalga-Diatom
Ptg transporter MMETSP0149-20130528_2569 MM31A-1 Chaetoceros debilis Microalga-Diatom
Ptg transporter MMETSP0150-20130528_28680 MM31A-1 Chaetoceros debilis Microalga-Diatom
Ptg transporter MMETSP0150-20130528_5247 MM31A-1 Chaetoceros debilis Microalga-Diatom
Ptg transporter MMETSP1447-20131203_66063 CCMP1751 Chaetoceros dichaeta Microalga-Diatom
Ptg transporter MMETSP0751-20121128_20690 CCMP1317 Chaetoceros neogracile Microalga-Diatom
Ptg transporter MMETSP0751-20121128_4430 CCMP1317 Chaetoceros neogracile Microalga-Diatom
Ptg transporter MMETSP0752-20121128_16154 CCMP1317 Chaetoceros neogracile Microalga-Diatom
Ptg transporter MMETSP0754-20121128_4267 CCMP1317 Chaetoceros neogracile Microalga-Diatom
Ptg transporter MMETSP0754-20121128_6631 CCMP1317 Chaetoceros neogracile Microalga-Diatom
Ptg transporter MMETSP0200_2-20121128_13609 GSL56 Chaetoceros sp Microalga-Diatom
Ptg transporter MMETSP0200_2-20121128_1933 GSL56 Chaetoceros sp Microalga-Diatom
Ptg transporter MMETSP0200_2-20121128_3645 GSL56 Chaetoceros sp Microalga-Diatom
Ptg transporter MMETSP1429-20130617_78154 UNC1202 Chaetoceros sp Microalga-Diatom
Ptg transporter MMETSP1429-20130617_8616 UNC1202 Chaetoceros sp Microalga-Diatom
Ptg transporter MMETSP0010_2-20120614_11436 308 Corethron hystrix Microalga-Diatom
Ptg transporter MMETSP0010_2-20120614_18205 308 Corethron hystrix Microalga-Diatom
Ptg transporter MMETSP0010_2-20120614_5483 308 Corethron hystrix Microalga-Diatom
Ptg transporter MMETSP0169-20130528_5424 L29A3 Corethron pennatum Microalga-Diatom
Ptg transporter MMETSP0169-20130528_61601 L29A3 Corethron pennatum Microalga-Diatom
Ptg transporter MMETSP0171-20130528_107041 L29A3 Corethron pennatum Microalga-Diatom
Ptg transporter MMETSP1066-20121228_134602 CCMP2513 Coscinodiscus wailesii Microalga-Diatom
Ptg transporter MMETSP0397-20121206_3438 ECT3854 Cyclophora tenuis Microalga-Diatom
Ptg transporter MMETSP1057-20121228_10109 CCMP338 Cyclotella meneghiniana Microalga-Diatom
Ptg transporter MMETSP1057-20121228_12627 CCMP338 Cyclotella meneghiniana Microalga-Diatom
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Ptg transporter MMETSP1057-20121228_2242 CCMP338 Cyclotella meneghiniana Microalga-Diatom
Ptg transporter MMETSP0017_2-20120614_25628 KMMCC:B-181 Cylindrotheca closterium Microalga-Diatom
Ptg transporter MMETSP0017_2-20120614_8292 KMMCC:B-181 Cylindrotheca closterium Microalga-Diatom
Ptg transporter MMETSP0017_2-20120614_9474 KMMCC:B-181 Cylindrotheca closterium Microalga-Diatom
Ptg transporter MMETSP0580-20130426_41845 Dactyliosolen fragilissimus
Microalga-Diatom
Ptg transporter MMETSP0580-20130426_6520 Dactyliosolen fragilissimus
Microalga-Diatom
Ptg transporter MMETSP1058-20130122_5998 CCMP353 Detonula confervacea Microalga-Diatom
Ptg transporter MMETSP1058-20130122_60219 CCMP353 Detonula confervacea Microalga-Diatom
Ptg transporter MMETSP1437-20131217_3447 CCMP1452 Eucampia antarctica Microalga-Diatom
Ptg transporter MMETSP1437-20131217_45704 CCMP1452 Eucampia antarctica Microalga-Diatom
Ptg transporter MMETSP1437-20131217_46324 CCMP1452 Eucampia antarctica Microalga-Diatom
Ptg transporter MMETSP0733-20121128_115 L26-C5 Fragilariopsis kerguelensis
Microalga-Diatom
Ptg transporter MMETSP0734-20130129_32869 L26-C5 Fragilariopsis kerguelensis
Microalga-Diatom
Ptg transporter MMETSP0734-20130129_45816 L26-C5 Fragilariopsis kerguelensis
Microalga-Diatom
Ptg transporter MMETSP0736-20130129_7353 L26-C5 Fragilariopsis kerguelensis
Microalga-Diatom
Ptg transporter MMETSP0907-20130614_129340 L2-C3 Fragilariopsis kerguelensis
Microalga-Diatom
Ptg transporter MMETSP0908-20130614_113276 L2-C3 Fragilariopsis kerguelensis
Microalga-Diatom
Ptg transporter MMETSP0009_2-20130614_86438 CCMP410 Grammatophora oceanica
Microalga-Diatom
Ptg transporter MMETSP0009_2-20130614_9365 CCMP410 Grammatophora oceanica
Microalga-Diatom
Ptg transporter MMETSP0322-20121206_16142 B651 Leptocylindrus apora Microalga-Diatom
Ptg transporter MMETSP0322-20121206_6063 B651 Leptocylindrus apora Microalga-Diatom
Ptg transporter MMETSP1362-20130617_58757 CCMP1856 Leptocylindrus danicus Microalga-Diatom
Ptg transporter MMETSP1362-20130617_9502 CCMP1856 Leptocylindrus danicus Microalga-Diatom
Ptg transporter MMETSP1360-20130828_11970 CCMP2313 Licmophora paradoxa Microalga-Diatom
Ptg transporter MMETSP1360-20130828_13532 CCMP2313 Licmophora paradoxa Microalga-Diatom
Ptg transporter MMETSP1360-20130828_76860 CCMP2313 Licmophora paradoxa Microalga-Diatom
Ptg transporter MMETSP1070-20121207_135 NH13 Minutocellus polymorphus
Microalga-Diatom
Ptg transporter MMETSP1070-20121207_5496 NH13 Minutocellus polymorphus
Microalga-Diatom
Ptg transporter MMETSP1070-20121207_6207 NH13 Minutocellus polymorphus
Microalga-Diatom
Ptg transporter MMETSP1322-20130828_30754 RCC2270 Minutocellus polymorphus
Microalga-Diatom
Ptg transporter MMETSP1322-20130828_31343 RCC2270 Minutocellus polymorphus
Microalga-Diatom
Ptg transporter MMETSP0744-20121128_13503 CCMP561 Nitzschia punctata Microalga-Diatom
Ptg transporter MMETSP0745-20121128_1905 CCMP561 Nitzschia punctata Microalga-Diatom
Ptg transporter MMETSP0747-20121128_11634 CCMP561 Nitzschia punctata Microalga-Diatom
Ptg transporter MMETSP0014_2-20120614_15610 RCC80 Nitzschia sp Microalga-Diatom
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Ptg transporter MMETSP0014_2-20120614_19139 RCC80 Nitzschia sp Microalga-Diatom
Ptg transporter MMETSP0014_2-20120614_46247 RCC80 Nitzschia sp Microalga-Diatom
Ptg transporter MMETSP0015_2-20120614_39172 isolate 1302-5 Odontella aurita Microalga-Diatom
Ptg transporter MMETSP0015_2-20120614_6159 isolate 1302-5 Odontella aurita Microalga-Diatom
Ptg transporter MMETSP0160_2-20130417_9596 Grunow 1884 Odontellasinensis Microalga-Diatom
Ptg transporter MMETSP0174-20130528_123001 PI-D3 Proboscia alata Microalga-Diatom
Ptg transporter MMETSP0174-20130528_124643 PI-D3 Proboscia alata Microalga-Diatom
Ptg transporter MMETSP0176-20130528_126607 PI-D3 Proboscia alata Microalga-Diatom
Ptg transporter MMETSP0176-20130528_129569 PI-D3 Proboscia alata Microalga-Diatom
Ptg transporter MMETSP0816-20121228_116198 CCAP1064/1 Proboscia inermis Microalga-Diatom
Ptg transporter MMETSP0816-20121228_117849 CCAP1064/1 Proboscia inermis Microalga-Diatom
Ptg transporter MMETSP0816-20121228_18613 CCAP1064/1 Proboscia inermis Microalga-Diatom
Ptg transporter MMETSP0329-20121206_18166 B593 Pseudo-nitzschia arenysensis
Microalga-Diatom
Ptg transporter MMETSP0329-20121206_8660 B593 Pseudo-nitzschia arenysensis
Microalga-Diatom
Ptg transporter MMETSP0329-20121206_993 B593 Pseudo-nitzschia arenysensis
Microalga-Diatom
Ptg transporter MMETSP0139_2-20121125_8846 10249 10 AB Pseudo-nitzschia australis
Microalga-Diatom
Ptg transporter MMETSP0141_2-20121125_126 10249 10 AB Pseudo-nitzschia australis
Microalga-Diatom
Ptg transporter MMETSP0142_2-20121227_8395 10249 10 AB Pseudo-nitzschia australis
Microalga-Diatom
Ptg transporter MMETSP1060-20121228_6609 Pseudo-nitzschia cingulata
Microalga-Diatom
Ptg transporter MMETSP1060-20121228_68874 Pseudo-nitzschia cingulata
Microalga-Diatom
Ptg transporter MMETSP1060-20121228_69106 Pseudo-nitzschia cingulata
Microalga-Diatom
Ptg transporter MMETSP0327-20121206_2559 B596 Pseudo-nitzschia delicatissima
Microalga-Diatom
Ptg transporter MMETSP0327-20121206_4244 B596 Pseudo-nitzschia delicatissima
Microalga-Diatom
Ptg transporter MMETSP0327-20121206_6469 B596 Pseudo-nitzschia delicatissima
Microalga-Diatom
Ptg transporter MMETSP0850-20130426_54709 WWA7 Pseudo-nitzschia fraudulenta
Microalga-Diatom
Ptg transporter MMETSP0851-20130426_132905 WWA7 Pseudo-nitzschia fraudulenta
Microalga-Diatom
Ptg transporter MMETSP0851-20130426_190132 WWA7 Pseudo-nitzschia fraudulenta
Microalga-Diatom
Ptg transporter MMETSP0851-20130426_3188 WWA7 Pseudo-nitzschia fraudulenta
Microalga-Diatom
Ptg transporter MMETSP1423-20130617_26482 UNC1101 Pseudo-nitzschia heimii Microalga-Diatom
Ptg transporter MMETSP1423-20130617_4130 UNC1101 Pseudo-nitzschia heimii Microalga-Diatom
Ptg transporter MMETSP1423-20130617_720 UNC1101 Pseudo-nitzschia heimii Microalga-Diatom
Ptg transporter comp10879_c0_seq1 Female small size
Pseudo-nitzschia multistriata
Microalga-Diatom
Ptg transporter comp14879_c0_seq3 Female small size
Pseudo-nitzschia multistriata
Microalga-Diatom
Ptg transporter comp14879_c0_seq4 Female small size
Pseudo-nitzschia multistriata
Microalga-Diatom
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Ptg transporter comp14879_c0_seq5 Female small size
Pseudo-nitzschia multistriata
Microalga-Diatom
Ptg transporter comp8763_c0_seq2 Female small size
Pseudo-nitzschia multistriata
Microalga-Diatom
Ptg transporter MMETSP0789-20121207_14424 CCMP1694 Rhizosolenia setigera Microalga-Diatom
Ptg transporter MMETSP0789-20121207_25487 CCMP1694 Rhizosolenia setigera Microalga-Diatom
Ptg transporter MMETSP0789-20121207_2838 CCMP1694 Rhizosolenia setigera Microalga-Diatom
Ptg transporter MMETSP0789-20121207_5660 CCMP1694 Rhizosolenia setigera Microalga-Diatom
Ptg transporter MMETSP0789-20121207_7461 CCMP1694 Rhizosolenia setigera Microalga-Diatom
Ptg transporter MMETSP0013_2-20120614_20568 1716 Skeletonema costatum Microalga-Diatom
Ptg transporter MMETSP0013_2-20120614_3130 1716 Skeletonema costatum Microalga-Diatom
Ptg transporter MMETSP0013_2-20120614_35941 1716 Skeletonema costatum Microalga-Diatom
Ptg transporter MMETSP0013_2-20120614_6102 1716 Skeletonema costatum Microalga-Diatom
Ptg transporter MMETSP0562-20121206_3835 SkelB Skeletonema dohrnii Microalga-Diatom
Ptg transporter MMETSP0563-20130528_11575 SkelB Skeletonema dohrnii Microalga-Diatom
Ptg transporter MMETSP0563-20130528_57923 SkelB Skeletonema dohrnii Microalga-Diatom
Ptg transporter MMETSP0578-20130828_59445 CCMP1804 Skeletonema grethea Microalga-Diatom
Ptg transporter MMETSP0593-20130828_6310 CCMP2506 Skeletonema japonicum Microalga-Diatom
Ptg transporter MMETSP0319-20121206_7134 SM1012Hels-07 Skeletonema marinoi Microalga-Diatom
Ptg transporter MMETSP0320-20121206_143 SM1012Den-03 Skeletonema marinoi Microalga-Diatom
Ptg transporter MMETSP0320-20121206_18510 SM1012Den-03 Skeletonema marinoi Microalga-Diatom
Ptg transporter MMETSP0320-20121206_25798 SM1012Den-03 Skeletonema marinoi Microalga-Diatom
Ptg transporter MMETSP1039-20121108_17278 FE7 Skeletonema marinoi Microalga-Diatom
Ptg transporter MMETSP0603-20130417_70287 CCMP793 Skeletonema menzelii Microalga-Diatom
Ptg transporter MMETSP0603-20130417_71813 CCMP793 Skeletonema menzelii Microalga-Diatom
Ptg transporter MMETSP0604-20130417_72559 CCMP793 Skeletonema menzelii Microalga-Diatom
Ptg transporter MMETSP0604-20130417_72711 CCMP793 Skeletonema menzelii Microalga-Diatom
Ptg transporter MMETSP1361-20130828_34096 CCMP2646 Staurosira complex Microalga-Diatom
Ptg transporter MMETSP1361-20130828_4670 CCMP2646 Staurosira complex Microalga-Diatom
Ptg transporter MMETSP0794_2-20130614_50572 CCMP 815 Stephanopyxis turris Microalga-Diatom
Ptg transporter MMETSP0794_2-20130614_50856 CCMP 815 Stephanopyxis turris Microalga-Diatom
Ptg transporter MMETSP0794_2-20130614_52112 CCMP 815 Stephanopyxis turris Microalga-Diatom
Ptg transporter MMETSP0800-20121206_177 CCMP2910 Striatella unipunctata Microalga-Diatom
Ptg transporter MMETSP0786-20121207_3412 CCMP1798 Thalassionema frauenfeldii
Microalga-Diatom
Ptg transporter MMETSP0786-20121207_9273 CCMP1798 Thalassionema frauenfeldii
Microalga-Diatom
Ptg transporter MMETSP0156-20130528_26157 L26-B Thalassionema nitzschioides
Microalga-Diatom
Ptg transporter MMETSP0158-20130606_26254 L26-B Thalassionema nitzschioides
Microalga-Diatom
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Ptg transporter MMETSP0158-20130606_7722 L26-B Thalassionema nitzschioides
Microalga-Diatom
Ptg transporter MMETSP0902-20130614_56002 CCMP982 Thalassiosira antarctica Microalga-Diatom
Ptg transporter MMETSP0905-20130828_4447 CCMP982 Thalassiosira antarctica Microalga-Diatom
Ptg transporter MMETSP0905-20130828_65551 CCMP982 Thalassiosira antarctica Microalga-Diatom
Ptg transporter MMETSP0493-20130528_38175 GMp14c1 Thalassiosira gravida Microalga-Diatom
Ptg transporter MMETSP0493-20130528_45824 GMp14c1 Thalassiosira gravida Microalga-Diatom
Ptg transporter MMETSP0494-20130426_49330 GMp14c1 Thalassiosira gravida Microalga-Diatom
Ptg transporter MMETSP0494-20130426_52164 GMp14c1 Thalassiosira gravida Microalga-Diatom
Ptg transporter MMETSP0737-20130205_1557 CCMP1093 Thalassiosira miniscula Microalga-Diatom
Ptg transporter MMETSP0737-20130205_66028 CCMP1093 Thalassiosira miniscula Microalga-Diatom
Ptg transporter MMETSP0737-20130205_67342 CCMP1093 Thalassiosira miniscula Microalga-Diatom
Ptg transporter MMETSP0970-20121206_8045 CCMP1005 Thalassiosira oceanica Microalga-Diatom
Ptg transporter MMETSP0971-20121206_10945 CCMP1005 Thalassiosira oceanica Microalga-Diatom
Ptg transporter MMETSP0971-20121206_1703 CCMP1005 Thalassiosira oceanica Microalga-Diatom
Ptg transporter MMETSP0972-20121206_24576 CCMP1005 Thalassiosira oceanica Microalga-Diatom
Ptg transporter MMETSP1067-20121228_139476 Tpunct2005C2 Thalassiosira puntigera Microalga-Diatom
Ptg transporter MMETSP1067-20121228_14711 Tpunct2005C2 Thalassiosira puntigera Microalga-Diatom
Ptg transporter MMETSP0403-20120918_14596 CCMP3096 Thalassiosira rotula Microalga-Diatom
Ptg transporter MMETSP0403-20120918_2990 CCMP3096 Thalassiosira rotula Microalga-Diatom
Ptg transporter MMETSP0910-20130528_2098 GSO102 Thalassiosira rotula Microalga-Diatom
Ptg transporter MMETSP0910-20130528_34273 GSO102 Thalassiosira rotula Microalga-Diatom
Ptg transporter MMETSP1059-20121228_3048 FW Thalassiosira sp Microalga-Diatom
Ptg transporter MMETSP1059-20121228_75934 FW Thalassiosira sp Microalga-Diatom
Ptg transporter MMETSP1071-20121207_17545 NH16 Thalassiosira sp Microalga-Diatom
Ptg transporter MMETSP1071-20121207_6197 NH16 Thalassiosira sp Microalga-Diatom
Ptg transporter MMETSP1071-20121207_8979 NH16 Thalassiosira sp Microalga-Diatom
Ptg transporter MMETSP0881-20121228_4845 CCMP1336 Thalassiosira weissflogii Microalga-Diatom
Ptg transporter MMETSP1407-20130829_41626 CCMP1010 Thalassiosira weissflogii Microalga-Diatom
Ptg transporter MMETSP1412-20130829_53321 CCMP1010 Thalassiosira weissflogii Microalga-Diatom
Ptg transporter MMETSP1413-20130829_48134 CCMP1010 Thalassiosira weissflogii Microalga-Diatom
Ptg transporter MMETSP1416-20130829_56987 CCMP1010 Thalassiosira weissflogii Microalga-Diatom
Ptg transporter MMETSP1419-20130829_2779 CCMP1010 Thalassiosira weissflogii Microalga-Diatom
Ptg transporter MMETSP1422-20130829_39721 CCMP1010 Thalassiosira weissflogii Microalga-Diatom
Ptg transporter MMETSP0152-20130528_78003 L6-D1 Thalassiothrix antarctica Microalga-Diatom
Ptg transporter MMETSP0152-20130528_8369 L6-D1 Thalassiothrix antarctica Microalga-Diatom
Ptg transporter MMETSP0154-20130528_76871 L6-D1 Thalassiothrix antarctica Microalga-Diatom
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Transcripts sequences of the Skeletonema marinoi prostaglandin pathway reported in the main text.
>COX-1_Skel_mar_MMETSP1039-20121108|4563_1 len=1758
ATGAAGGCAGCAGCCAGCATCATCGTAGCCGTCATCGGCATCGTAATCTTTTACCTCAAACAAGCCAGTCTTCCATCCACCGCGCCCACACCACCAGGCTACCCCCTCCCACCCGACAGCATTGAATGGACGAGCCCACCCTATCCCTTTACAGAATGGCTCATCGACACTTTAAAGGCTCCAGTCATCAACTTCGGGTTGAGTCTGCCCGTCATATCTAGTTTTGTGAACCGTCGTCTACAGAACAGGGCAAGCACCAGCGGTTCCAATCGTCCATACAATCTATCCTGTAAATCGGACTACACCTCGTGGGATTCATTGAACGACCGTTCCTACTTTGGTAGACACTTGCCACCCAAAGAAATCCCTGATTTGCCACCTGTCGATGAAGTGGTGAACAAACTATTCCCACGCAAAGATGGCATCCAGACCATGTGTCCAAGATCAACACTTCTTTTTCCAACATTTGCCCAACATTTGATTGATTCGTTTATCAATACGAAAATTAATGTGGAGACTGGTGAGTTTGAGTGGGATAGAACCGATAGCAAGCACGAGATTGGGATAGGTCCTCTGTATGGGGATGAGGTAGAACAGACGAGTCAACTTCGTGAAAAGAGTGAAAAACCTGGGCGGAGGGGACGGCTTAAGACTCAAGTGTTGGAAGGTGGGGAAGAATGGGCACCTTTTCTCTATAATGAAGATGGCACCAAGAAGAAGGAGTTCAGTGCAATCCATGATCCTGATGGGATGAAAACTATCCTTGGGTTGGTCTACAGTTCTGATCCTACCACCAAAAGTTCGATTGAGCAGTCCATCTTTGCATTTGGAGGAAGACGTGCTAATTTGAATCCTAATATCGTTGCATGGAACACTCTCTTGTTGAGGGAGCATAATCGTCTTGCGGGGGAGATTGAGAAGAGTGAACCGGGGTGGGATGATGAACGTGTGTTTCAGACTGCTCGTAATGTCCTTATTGTTATGTATTGCAAGATTGTCATTGAAGAATACATCAAGCACATCAGTGGTGTAAACTTCAAAGTCGAGCCAGGTCCTTGGATGTGGAATGCACCCTGGTACAAAACCAATTGGATGTCTACCGAGTTTGCAATCTTGTATCGCTGGCACGCTCTCATTCCCAATGAGGCTGGTCTAGGTCCAAGCAAGGATGCAGGAGTAATGGAGGCACTATTCAATAATCCTATGTTGCTTGATGACGAGACTGGCCTGGGTGGCAACCTACGTGATATCTTTGTGGACATTAGTCAGACTCGGGTTACATCCTTACAATTGTTCAACACCGAGAAGTGGATGGTTGAACGTGAATCAGCAGCTATCAATCAGGGCCGTGCGAACAATGTTCAGAGTTATGCCGCATACTGCGAATACCTTGACATTGAACCACCCAAGACGTTTGAGGAYATTTCAATGGTGCCTGAAAGGCAGCAAGCCTTGAAGGAGCTTTATGGTACTCCAGATAGGGTAGAATTCTATGTTGGTTTGATTGCGGCAGATCATCCTGCAGGTGGGAAAATCTTTTCAGAGGCCATGACCAAGTTTGTAGCCAACGATGCCTTTAATCAAGCGTTAACCAACCCTCTCCTCTCCCAAAATGTTTGGGAAAATGGTGAGGAGACGTTTGGAAAATATGGTTGGGAGGTGGTAAAGCAAGATCATACCGTCAGGGATCTTCTGGAAAGGAACATTCCAAATGGTCTTGGTGATGCTTTTATTGGGATGACCATTCCAACTCTTTGA
>PTGEs_Skel_mar_MMETSP1039-20121108|5744_1 len=1008
ATGCTCAAAACAACAACAGCAATGAATACTACTCTAAGGTGTTTCGTAGGGCGAGCAGCAGCCCGCACATCTGTGCATAGTCATCATCTAACATCAAAATCACAGCAGCAACTTGCTTTCGTTTCATCATTGCATTCACCATCAACATCGGCATGGCTTTCCAGTAATTATTCAGCAAGATCGTTCTCGGCGGCTKCAACTGGACAAGCACCAGAAATTAAACTCTACCAGTACCACATTTGTCCCTTTTGCAATATTACAAAATCAGTGCTRTCCTATGCCAAATTAGATTATGAGAAGGTGGAGGTAAATCCACTRACAAAACAAGAGTTGAAACCATGGTCAGGAGATTATAAAAAGGTTCCAATAGCTGTGATTGACGAAGAACAAGTGAATGGATCGGAACAAATCATCGATGCGCTACTTGATACACAATATGTGCAGCAATTTTTGCAAAGTCGATGGAATGATGAAGCTAGTGACAAAGATGYCATGACGATGCAACAATTTCAAAACTCGGAAAACTCCCAAAAGTGGATGAGATACGCTGTAGATGATCTTGCAGCSTTGTTGTATCCGAAYAT
42
367368
369
370371372373374375376377378379380381382383384385386387388389390391392393394395396397398399400
401
402403404405406407408409410411
8384
Page 43
TTGYGGTACWCTTGGKGAYTCTTACGATGCGTTTTCWTATGTCAAGAATGTAGATTCGTTTACTGGTGGTCAAAAGATATCAATTCAGATGCTGGGCTCTTTGGCCATGTACTTTGCAGCATCAAGAGTTAAGTCAAAACGAAACATTACGGATGAAAAGAAAGCATTGAAAGAAGCTCTTGACAAGTTTGAGCACGAGGGACTTGATAATGGAAAGTTATCCTTTTGTTCTGGTGTTTCAACACCTTATTTGGGTGATCTTGCCGTGTTTGGAGTCCTCTACTCTGTAAATGGTTTACAGGCTCATACAGATTCTATCCAGCTTCGAGGAGGTGCTGTGAAAGAATGGTATGATAGAATGCATCTCTCTGTGATGGGAGAAAAAGCGCAGCATCAGATCAAGCTTGGTGTCACTTGTGATTAA
>PTGDs or PtHi_Skel_mar_MMETSP1039-20121108|1431_1 len=648
XXCAACAATACAGACAATACAAACAACAATATGACTTCTTCTTCATCGCGACAGCACGAGCTCCTCTACTTCAACGGCGCCGGAAGAGCAGAAACAATTCGCATCTCACTCCACGCCGCTGGCATCAACTTCACCGACACGAGATTCACCGGCAAAGATTGGCCGGCGATCAAACCAACTACACCACTGGGCGGCGTACCCGTTCTCAAGGTAGATGGTGATTCACACTGTCAGTCTGTTTCGTTAGCCCGTTATGCTGGTAAGCTCGGTGGGTTTTATCCAACTGATCCCCTTCAGGCGCTTATTGTAGACGAGGCGATTGAAACTTTGAATGAGTTGATGGCAGTAGCCCCTAAACACTCTGATCCAGAAGAATTACTCAAGCTTCGTAAAGAGTTCCAAGCTGGCACAATGACAAAGTATATTGACTTCCTCGAGAGTTTGATTCAAAAGAATGGCGGAGTGGGATTTGCTTCTACCCCATCTATCGCCGATCTGACTCTTTGGGGTACAGTCAAGATGATTCGTACTGGGGATTGGACTGGAATTGATGCCACCTTCTTTGAATCTTATGCGGGTATTGTGGCGACTGTGGAGTCGATTGCCAAGGATGATAAGGTTGTTGCCTACTATGCATCGAGGGAGTAA
>PTGT_Skel_mar_MMETSP1039-20121108|17278_1 len=741
GGTTCATTGCCAGCGTTACCTGAAAGTGATGCTGATCTGTTGCAATCGGGTTGGCCATGGAGAGGGCATGTTGACAATCCTTCTTTGCCTCTTGTTCTGAAGAATGTATCAGTTGATATTCCCGCTGGTTCTACGCTAGGAGTTGTCGGCCGAACAGGAAGTGGAAAGAGTTCCCTCTTGCTAACTCTATTCCGTCTTGCTGAGATTGAGGGCAACGCAGGAAGTAGCATCACAATTGATGGGGTTGATATCAGATCACTCAGCCTTCAAGGTCTACGAGATTCAATATCTATCATCCCCCAAACTCCGATTCTTTTTGCTGGAACATTAATGCACAATTTAGATGCATCAGGGAAGGCCACGCCAGAGGATGCTTGGAGAGCATTGGAGTCAGCAAGTCCACAGCTCGCCAAACAGTTTAGAGACTCCAGCGAAGGCTTGAACACAAACATTTCAGAGGGTGGCGAAAACCTTTCTCTTGGTCAAAGACAGCTGATTTGTCTGGCAAGGGCATTAATGAAAAGGAGCAAAATTTTAGTTCTTGACGAGGCTACAAGTAGTATCGACATGAAAACTGATGCCCAAGTTCAAGAGACAATCAGGCGAGAGTTCGTTCAAAAAGGAGTGACAGTAATAACTGTTGCTCATAGATTAGAGACAGTGCTTGGGTACGATAGAATCCTTGTGCTTGATGCTGGCGTTCCAGTTGAGTTTGGCCCACCGGATGAACTCTTGAGGCGX
43
412413414415416417418419
420
421422423424425426427428429430431432
433
434435436437438439440441442443444445446
447
448
449
450
451
452
8586