Mechanical Properties of Collagen Molecule

Mechanical Properties of Proteins

Swapnil Khachane

Take Away

1. What are proteins

2. Necessity of the study

3. Sarcomere and Titin

4. Go-like Model

5.Results

6.Summary

Purpose of the Study

• Diseases

• Injury/Trauma

• Future Inventions

Titin

From. Cieplak 2002Figure 1. Structure of Titin

Titin

Figure 2. Structure of TitinFrom. Tshovorebova 2005

Sarcomere in a Striated Muscle

From. Cieplak 2002Figure 3. Sarcomere

Go Like Model

From. Buehler 2008

• Cα Atoms

• Lennard Jones Potential

• Equation of Motion

612

ij 24 ij

ij

ij

ij

rrE

randFFcrrm

Results from the model

Figure 4. Force Displacement Curve for 1titFrom. Cieplak 2005

Experimental Results

Figure 5. Force Displacement Curve for 3tit

From. Tshovorebova 2005

Experimental Results

Figure 6. Force Displacement Curve for 3tit

From. Tshovorebova 2005

References•Sulkowska et al., Predicting the order in which contacts are broken during single molecule protein stretching experiments, Wiley-Liss Inc, 2007.•Cieplak et al. , Folding and Stretching in a Go-like Model of Titin.•Cieplak et al,. Stretching of proteins in the entropic limit. Phys Rev e 2004; 69: 011912.•Erickson HP. Protein biophysics - stretching single protein molecules: titin is a weird spring. Science 1997; 276: 1090-1092.•Tskhovrebova et al, Elasticity and unfolding of single molecules of the giant muscle protein titin. Nature 1997; 387: 308-312.•Dietz H,Berkemeier F,Bertz M,Rief M. Anisotropic deformation response of single protein molecules. Proc Natl Acad Sci USA 2006; 103: 12724-12728.•West et al, Mechanical Resistance of Proteins Explained Using Simple Molecular Models, Biophysical Journal, Volume 90, Issue 1, 287-297, 1 January 2006.•Ceiplak et al, Pulling single bacteriorhodopsin out of a membrane: Comparison of simulation and experiment, Biochimica et Biophysica Acta (BBA) - Biomembranes•Volume 1758, Issue 4, April 2006, Pages 537-544.•Kil Ho Eom, The Mechanical Modeling of Proteins.•Yoon et al, Mesoscopic Model for Mechanical Characterization of Biological Protein Materials, Nano-Bio Research Center, Korea Institute of Science & Technology (KIST), Seoul 136-791, Republic of Korea.

Thank You