Measurement of Endo and Exo-Glucanas e Activities in Cellulase Using Non-Crystalline Cellulose Rajesh Gupta and Y. Y. Lee Rajesh Gupta and Y. Y. Lee Department of Chemical Engineering Department of Chemical Engineering Auburn University, AL 36849 Auburn University, AL 36849 [email protected][email protected]AIChE Annual Meeting, San Francisco, CA AIChE Annual Meeting, San Francisco, CA November 16, 2006 November 16, 2006
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Measurement of Endo and Exo-Glucanase Activities in Cellulase Using Non-Crystalline Cellulose Rajesh Gupta and Y. Y. Lee Department of Chemical Engineering.
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Measurement of Endo and Exo-Glucanase Activities in Cellulase
Using Non-Crystalline Cellulose
Rajesh Gupta and Y. Y. LeeRajesh Gupta and Y. Y. Lee
Department of Chemical EngineeringDepartment of Chemical Engineering
Auburn University, AL 36849Auburn University, AL 36849
AIChE Annual Meeting, San Francisco, CAAIChE Annual Meeting, San Francisco, CA
November 16, 2006November 16, 2006
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OutlineOutline
Overview on Cellulosic EthanolOverview on Cellulosic Ethanol Significance of Cellulase EnzymesSignificance of Cellulase Enzymes Cellulase Action and ActivitiesCellulase Action and Activities
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BIOFUELBIOFUEL
““Sustainable transportation fuel made from Sustainable transportation fuel made from renewable feedstock”renewable feedstock”
Biofuels present opportunities for new Biofuels present opportunities for new industry and improved farm economy.industry and improved farm economy.
The fuel ethanol industry alone adds more The fuel ethanol industry alone adds more than $20 billion/year to the US economy.than $20 billion/year to the US economy.
Why Why BiofuelsBiofuels??
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EthanolEthanol BoomBoom
US produced 3.9 billion gallons of BioethaUS produced 3.9 billion gallons of Bioethanol in 2005 (about 3% of US gasoline consnol in 2005 (about 3% of US gasoline consumption) exclusively from corn. umption) exclusively from corn.
30 new plants and 9 expansions are curre30 new plants and 9 expansions are currently under way(=additional 1.8 billion gallontly under way(=additional 1.8 billion gallons/year capacity). ns/year capacity).
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Economic Impact of Corn Based Economic Impact of Corn Based EthanolEthanol
Goals of Renewable Fuel Standard (2005):Goals of Renewable Fuel Standard (2005):
8 billion gallons by 2012.8 billion gallons by 2012. Ethanol industry will spend $70 billion over 2005-Ethanol industry will spend $70 billion over 2005-
2012.2012. Direct spending and indirect impact will add $200 Direct spending and indirect impact will add $200
billion to GDP over 2005-2012.billion to GDP over 2005-2012. Reduce trade deficit by $64 billion.Reduce trade deficit by $64 billion.
Long term goal: 20 billon gallons by 2020. Long term goal: 20 billon gallons by 2020.
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Constraints of Corn-Based Constraints of Corn-Based EthanolEthanol
Upper limit? Upper limit?
8 billion gallons/year represents 1/3 of total 8 billion gallons/year represents 1/3 of total corn production.corn production.
Marketability of byproducts (DDG).Marketability of byproducts (DDG).
Feedstock other than corn must be Feedstock other than corn must be utilized.utilized.
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Inherent Engineering DifficultiesInherent Engineering Difficultiesin Cellulosic Ethanol in Cellulosic Ethanol
Uses filter paper (Whatman No.1) as the standard Uses filter paper (Whatman No.1) as the standard substrate. substrate.
Initial rate is measured by one data-point. Initial rate is measured by one data-point.
Released sugars are measured in terms of reducing ends Released sugars are measured in terms of reducing ends by DNS reagent (does not distinguish G1 and G2). by DNS reagent (does not distinguish G1 and G2).
Repeatability is poor because of several factors in Repeatability is poor because of several factors in the procedure that are error-prone.the procedure that are error-prone.
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HPLC for measurement of released sugarHPLC for measurement of released sugar G1 & G2 are converted to glucan for G1 & G2 are converted to glucan for
conversion calculation. conversion calculation. Slope-method (multiple points) for initial rateSlope-method (multiple points) for initial rate
Proposed ModificationProposed Modification
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Activity Determination by initial slope method
Substrate: Filter Paper
Enzyme Loading: 0.01ml/g glucan
y = 0.0128x + 0.0002
y = 0.0114x + 0.0009
y = 0.0168x + 0.0008
0.00%
0.20%
0.40%
0.60%
0.80%
1.00%
1.20%
1.40%
1.60%
1.80%
2.00%
0 0.2 0.4 0.6 0.8 1 1.2
Time(hrs)
%G
luca
n Spezyme CP A
Spezyme Cp B
GC 220
Enzyme Loading: 0.005ml/g glucan
y = 0.007x + 0.0005
y = 0.005x + 0.0016
y = 0.0092x + 0.0013
0.00%
0.20%
0.40%
0.60%
0.80%
1.00%
1.20%
0 0.2 0.4 0.6 0.8 1 1.2
Time(hrs)
%G
luca
n Spezyme CP A
Spezyme Cp B
GC 220
Enzyme Loading (ml/g glucan)
Spezyme CP A Spezyme CP B GC 220
0.01 1.00 0.89 1.31
0.005 1.00 0.71 1.31
Average 1.00 0.89 1.31
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Beyond the FPU?Beyond the FPU?
Observation of G1 & G2 is not sufficient to Observation of G1 & G2 is not sufficient to characterize the cellulase.characterize the cellulase.
Different combination of the three cellulase Different combination of the three cellulase
components may give same FPU.components may give same FPU. Use of substrates with different properties Use of substrates with different properties
may provide additional information.may provide additional information.
• Amorphous cellulose made in our laboratory Amorphous cellulose made in our laboratory
from crystalline cellulose.from crystalline cellulose.• Hydrogen-bonds in cellulose are disrupted.Hydrogen-bonds in cellulose are disrupted.• Crystallinity is essentially removed.Crystallinity is essentially removed.
Schematic Representation of Structural Differences Schematic Representation of Structural Differences between Crystalline Cellulose & NCCbetween Crystalline Cellulose & NCC
Amorphous domain (Substrate for Endo-glucanase)
Reducing Ends(Susbtrate for Exo-glucanase)
Amorphous domain(Substrate for Endo-glucanase)
Reducing Ends(Susbtrate for Exo-glucanase)
E + S Pk
dP= k[E][S]
dt
E Pk’ = k[S]
dP= K’[E]
dt
Crystalline Cellulose NCC
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SOLIDLIQUID
Low DP NCC
COS
CELLOBIOSE
GLUCOSE
Β-G
NCC
Hydrolysis of NCC
Exo-G
Endo-G
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0.5ml 2% sonicated NCC Solution with Buffer0.5ml of diluted Enzyme Solution
Stop the reaction by boiling it for 5min. &
Dilute it 3 times by cold DI water
Stir the content and take two 0.5ml sample containing
uniform mixture liquid and solid
Take liquid samplefor finding the sugar concentration
(From HPLC)
Find the Glucose and Cellobioseconcentration
Incubate at 50oCfor 30min.
Add 3ml DNS reagent in one sample as it is and centrifuge other sample & then add 3ml of DNS in liquid part only. Boil both the sample for exact 5min and cool
the content Immediately. Centrifuge both the sample again and find the absorbance of both
( Solid+Liquid part and only Liquid part )
Measurement of Endo-Glucanase and Exo-Glucanase Activities from NCC
0.5ml 2% sonicated NCC Solution with Buffer0.5ml of diluted Enzyme Solution
Stop the reaction by boiling it for 5min. &
Dilute it 3 times by cold DI water
Stir the content and take two 0.5ml sample containing
uniform mixture liquid and solid
Take liquid samplefor HPLC
sugar analysis
Determine Glucose and Cellobioseconcentration
Incubate at 50oCfor 30min.
Add 3ml DNS reagent in one sample and centrifuge other sample & then add 3ml of DNS in liquid
part only. Boil both the sample for 5min and cool Immediately afterward. Centrifuge the sample
and find the absorbance of the Solid+Liquid part and the Liquid part.
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Activity Determination
Sample calculation
* HPLC results represent mg of sugars generated from the reaction.
Acid Hydrolysis of Acid Hydrolysis of Cello-oligosaccharidesCello-oligosaccharides
Cello-oligosaccharides
Ce
llob
iose
Glu
cose
Glu
cose
4% H2SO4, 121C, 1 hr
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Enzymatic Hydrolysis Enzymatic Hydrolysis of Cello-oligosaccharidesof Cello-oligosaccharides
Cello-oligosaccharides
Cellobios
e
Glucos
e
Cellobios
e
Glu
cose
Cello-oligosaccharides
Enzymatic Hydrolysis15FPU/g-glucan
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Hydrolysis of cello-oligosaccharide by Hydrolysis of cello-oligosaccharide by ββ-glucosidase (-glucosidase (Novo-188Novo-188))
Hydrolysis of Cello-oligosaccharides
0%
5%
10%
15%
20%
25%
30%
35%
0 20 40 60 80 100 120
Time (Hours)
% G
luc
an E
qu
iva
len
t)
Glucose (15 FPU+30 CBU)
Cellobiose (15 FPU+30 CBU)
Glucose (30 CBU)
Cellobiose (30 CBU)
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Hydrolysis of NCC and Cellobiose with Hydrolysis of NCC and Cellobiose with ββ-glucosidase-glucosidase
(Enzyme loading: 7 CBU/ g glucan)(Enzyme loading: 7 CBU/ g glucan)
Release of Glucose in NCC and Cellobiose
0%
4%
8%
12%
16%
20%
0 0.2 0.4 0.6 0.8 1
Time (Hours)
%G
luc
an
Eq
uiv
ale
nt
NCC
Cellobiose
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SummarySummary The economic, environmental, and The economic, environmental, and
strategic benefits of cellulosic ethanol are strategic benefits of cellulosic ethanol are great. great.
Time is ripe for commercial production of Time is ripe for commercial production of cellulosic ethanol.cellulosic ethanol.
Cost of the enzyme is a significant cost Cost of the enzyme is a significant cost factor in the cellulosic ethanol process.factor in the cellulosic ethanol process.
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oo
There is a room for improvement in the conventional FPU method.
The points to be addressed:
HPLC in place of reducing sugar. Calculate the extent of reaction in terms of the glucan equivalent of combined G1 and G2. Filter paper is still preferred over α-cellulose or Avicel because of consistency in property. Multiple-point (slope) method is preferred over one-point method for higher accuracy.
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Non-crystalline cellulose (NCC) can be used as a substrate to determine the relative activities of individual components of cellulase.
Hydrolysis of NCC by cellulase produces G1,G2,and cello-oligosacchrides (COS).
Formation of G1 and G2 from NCC may be taken as relative activity of exo-glucanase. Increase of reducing sugar in NCC may be taken as a measure of endo-glucanase activity.
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Cello-oligosaccharides (COS) can be used as Cello-oligosaccharides (COS) can be used as a substrate for identification of cellulase reactia substrate for identification of cellulase reactions.ons.
COS is hydrolyzed only by COS is hydrolyzed only by ββ-glucosidase.-glucosidase. (Endo and Exo-G cannot hydrolyze COS.)(Endo and Exo-G cannot hydrolyze COS.)
Hydrolysis of COS by cellulase is much slower Hydrolysis of COS by cellulase is much slower than NCC.than NCC.
ββ-Glucosidase works only on soluble substr-Glucosidase works only on soluble substratesates
(G2 & oligomeres).(G2 & oligomeres).
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AcknowledgementsAcknowledgements US Department of Energy Office of the BiomasUS Department of Energy Office of the Biomas
s Program (Contract DE-FG36-04GO14017)s Program (Contract DE-FG36-04GO14017) US EPA-TSE Program US EPA-TSE Program (No. RD-83164501) (No. RD-83164501) AU Team: Rajesh Gupta, Suma Peri, Yongming AU Team: Rajesh Gupta, Suma Peri, Yongming
Zhu, Hatem Harraz, T. H. Kim, Clayton SmithZhu, Hatem Harraz, T. H. Kim, Clayton Smith CAFI Team:CAFI Team:
Dartmouth College; Michigan State, Purdue, TDartmouth College; Michigan State, Purdue, Texas A&M; University of British Columbia; and exas A&M; University of British Columbia; and the National Renewable Energy Laboratorythe National Renewable Energy Laboratory