From the SelectedWorks of George McNamara Fall October 3, 2016 McNamara 20161006 revised draſt tables for CPHG Light Microscopy and Image Analysis.pdf George McNamara is work is licensed under a Creative Commons CC_BY International License. Available at: hps://works.bepress.com/gmcnamara/77/
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McNamara 20161006 revised draft tables for CPHG Light ...
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This work is licensed under a Creative Commons CC_BY International License
Available at httpsworksbepresscomgmcnamara77
Table 441 Useful Light Microscopy Web Sites
Light Microscopy (overview) Confocal Listserv (not just for confocal microscopes) httpslistsumneducgi-binwaA0=confocalmicroscopy G McNamara ndash Multi-Probe Microscopy (content last updated 2005 includes extensive glossary) httpsworksbepresscomgmcnamara2 Molecular Expressions National High Magnetic Field Laboratory (NHMFL) Florida State University httpmicromagnetfsuedu Microscopedia ndash Resources Guide To Light Microscopy httpwwwmicroscopediacomMicroscopyLight Molecular Probes Inc Invitrogen Thermo Fisher (Many links to useful and informative sites) httpswwwthermofishercomusenhomebrandsmolecular-probeshtml Photonics buyerrsquos guide (much more comprehensive than this list) httpwwwphotonicscomBuyersGuideaspx Science Exchange ndash Reproducibility Initiative (focus on commercial antibodies and uses) httpswwwscienceexchangecomapplicationsreproducibility Wikipedia - Chromosomes httpsenwikipediaorgwikiChromosome Wikipedia ndash Microscope httpenwikipediaorgwikiMicroscope Societies Association of Biomolecular Resource Facilities Light Microscopy Research Group (ABRF LMRG) httpsabrforgresearch-grouplight-microscopy-research-group-lmrg European Microscopy Society (EMS) httpwwweurmicsocorg International Society for Advancement of Cytometry (ISAC) httpisac-netorg Microscopy Society of America (MSA) httpwwwmicroscopyorg Royal Microscopical Society (RMS) httpwwwrmsorguk Major Microscope Companies Leica Microsystems Inc httpwwwleica-microsystemscom httpwwwleica-microsystemscomscience-lab httpwwwleica-microsystemscomproductsconfocal-microscopesdetailsproductleica-tcs-sp8-sted-3x
Karyotype Systems and Digital Pathology Digital Pathology Blog ndash see educational sponsors for comprehensive list of digital pathology vendors httptissuepathologycom 3DHistech ndash digital pathology Pannoramic scanners httpwww3dhistechcom Agilent Pathology Solutions Dako ndash IHC and ISH reagents autostainers httpwwwdakocomdkindexknowledgecenteromnishtm httpwwwdakocomdkar42productgroupshtm Applied Spectral Imaging ndash SKY Spectral karyotyping GenASIs digital pathology httpwwwspectral-imagingcom httpwwwspectral-imagingcomapplicationsspectral-imaging httpwwwspectral-imagingcomapplicationspathology Bioview ndash pathology and FISH cytogenetics scanners httpbioviewcom Definiens (MedImmuneAstraZeneca) - digital pathology image analysis (with deep learning algorithms) httpwwwdefinienscom Huron Digital Pathology ndash large area confocal scanning (entire human brain slices) httpwwwhurondigitalpathologycom Leica Biosystems ndash CytoVision CytogeneticsFISH Aperio digital pathology httpswwwleicabiosystemscomclinical-microscopy-surgery-radiologycytogenetics httpswwwleicabiosystemscomdigital-pathology MetaSystems International ndash karyotyping FISH digital pathology httpsmetasystems-internationalcomenproducts Molecular Probes ThermoFisher Scientific ndash Evos FL Auto and Evos Floid cell imaging stations high content screening (HCS) (Cellomics) httpswwwthermofishercomusenhomelife-sciencecell-analysiscellular-imagingcell-imaging-systemsevos-fl-autohtml httpswwwthermofishercomusenhomelife-sciencecell-analysiscellular-imagingcell-imaging-systemsfloid-cell-imaging-stationhtml httpswwwthermofishercomusenhomelife-sciencecell-analysiscellular-imaginghigh-content-screeninghigh-content-screening-instrumentshtml Philips ndash digital pathology httpwwwusaphilipscomhealthcaresolutionspathology Ventana Roche Digital Pathology ndash digital pathology FISH httpwwwventanacomdigitalpathology Visiopharm ndash digital pathology image analysis (with deep learning algorithms) stereology httpwwwvisiopharmcom
Live cell imaging dishes and multi-well plates (ldquoglass bottom dishesrdquo)
We recommend these for simplified handing and processing vs either thick plastic dishesplates or having to handle loose coverslips with your valuable cells
Leicester Imaging Technologies (LITE) - University of Leicester - table of dishes for live cell imaging (our table has current links and bad links left out) httpwww2leacukcollegesmedbiopsychfacilities-and-servicescbsliteaiftips-and-tricks-1dishes-for-live-cell-imaging Bellco Glass Inc - glass flat bottom plate httpwwwbellcoglasscomnode1046 Bioptechs - interchangable coverslip dish ($036 per experiment) httpwwwbioptechscomproductinterchangeable-coverslip-dish Brooks Automation Inc - clar bottom optical imaging microplates httpwwwbrookscomproductslife-scienceconsumablesmatrical-microplates-microtubes-racks-lidsglass-bottom-optical-imaging-microplates Cell EampG - glass bottom cell sulture dishes plates httpcellegcomproducthtml Cellvis - glass bottom dishes and plates httpwwwcellviscom_35-mm-glass-bottom-dishes_products_by_categoryphpcat_id=3 Corning - glass bottom microplates httpcatalog2corningcomLifeSciencesen-USShoppingCategoryaspxcategoryname=Microplates20-202015(Lifesciences)|Assay20Microplates20-202015(Lifesciences) Design 1 Solutions - Custom microplates and fabrication services httpwwwdesign1solutionscomcustom-microplateshtml Eppendorf - cell imaging dishes imaging plates httpsonline-shopeppendorfcomOC-enCell-Culture-and-Imaging-Consumables-110320Cell-Imaging-Consumables-120210Eppendorf-Cell-Imaging-Dishes-PF-19457html httpsonline-shopeppendorfcomOC-enCell-Culture-and-Imaging-Consumables-110320Cell-Imaging-Consumables-120210Eppendorf-Cell-Imaging-Plates-PF-19458html Greiner Bio-One International - CellView glass bottom dishes quadrant dishes httpsshopgbocomenrowarticlescataloguearticle0110_0110_0040_002027513 ibidi - u-Plates glass bottom dishes microfluidic slides httpibidicomxtproductsenibidi-Labwarem-Plates httpibidicomxtproductsenibidi-Labware Matsunami Glass Ind Ltd httpwwwmatsunami-glasscojpenglishlifebiodata01html Mattek - glass bottom dishes and SBS plates httpswwwmattekcomstore-categoryculturewareglass-bottom-dishes Nunc - glass bottom dishes httpswwwthermofishercomordercatalogproduct150680 NEST Biotechnology Co Ltd Wuxi Jiangsu PRChina httpwwwnestscientificusacomproducts_detailproductId=31html PerkinElmer - glass bottom plates httpwwwperkinelmercomSECatalogCategoryIDMicroplates
Porvair Sciences - clear bottom assay plates httpwwwporvair-sciencescomenservices-menulife-sciencesclear-bottom-assay-plates Ted Pella Inc - glass bottom dishes clear wall or black wall (manufactured by WillCo Wells) httpswwwtedpellacomsection_html706dishhtm Warner Instruments (Harvard Apparatus) - culture dishes perfusion systems httpswwwwarneronlinecomproduct_cat_infocfmid=1322 Willco Wells - glass bottom dishes wwwwillcowellscom World Precision Instruments - Fluorodish glass bottom dishes httpswwwwpiinccomproductslaboratory-suppliesfd35-100-fluorodish-cell-culture-dish-35mm-23mm-well-pkg-of-100 Microscopes Microscopy Accessories (accessorize) Reagents Resources 3scan ndash knife edge scanning microscope (images slices repeats through specimen) httpwww3scancomdata-2 89North (affiliated with Chroma Technology Corp) httpwww89northcom Abberior Instruments ndash STED RESOLFT precision localization super-resolution microscopes STEDYCON convert any fluorescence microscope to STED httpabberior-instrumentscom httpabberior-instrumentscomproductscompact-lineabberior-stedy-con Acquifer ndash HIVE imaging machine (computer hardware) for high content screening big data httpswwwacquiferde Adobe Systems Inc httpwwwAdobecom httpscreativeadobecomproductsdownloadphotoshop Affymetrix eBioscience (ThermoFisher) - microarrays httpwwwaffymetrixcomestorebrowselevel_one_category_template_onejspparent=35796ampcategory=35796 httpwwwaffymetrixcomestorecatalog131547AFFYGeneChip26231743B+System+3000Dx+v22623423B1_1 Agilent Technologies ndash SureScan microarray scanner (array CGH microRNA SNPs CNV) httpwwwgenomicsagilentcomenMicroarray-Scanner-Processing-HardwareSureScan-Microarray-Scannercid=AG-PT-144 Andor Technology (Oxford Instruments) ndash sCMOS cameras Dragonfly spinning disk confocal Revolution DSD2 differential spinning disk confocal photostimulation (fluorescence recovery after photobleaching optogenetics) Mosaic3 DMDDLP high speed light patterning (optogenetics structured illumination) MicroPoint pulsed UV laser for ablation DNA damage photobleachingactivation uncaging accessories httpwwwandorcomscientific-camerasneo-and-zyla-scmos-cameras httpwwwandorcommicroscopy-systemsdragonfly httpwwwandorcommicroscopy-systemsrevolution-dsd httpwwwandorcommicroscopy-systemsphotostimulation httpwwwandorcommicroscopy-systemsphotostimulationmosaic httpwwwandorcommicroscopy-systemsphotostimulationmicropoint httpwwwandorcommicroscopy-systemsmicroscopy-components Apogee Instruments Inc (Andor Technology) ndash CCD cameras and filter wheels (mostly marketed for astronomy) httpwwwccdcomandorhtml Applied Scientific Instrumentation Inc ndash light sheet imager (iSPIM diSPIM)
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
TGP (Thermal Green Protein) 493 507 64000 066 42 126
Topaz 514 527 94500 06 57 169
UnaG 498 527 77300 051 39 117
VafLOV 450 495 12500 023 3 009
Venus2 (V2) 515 528 184000 057 105 312
Venus3 (V3) 515 528 276000 057 157 468
Venus4 (V4) 515 528 368000 057 210 624
Venus5 (V5) 515 528 460000 057 262 780
Venus6 (V6) 515 528 552000 057 315 936
Violeta (GFPmut3W66) 350 425 na na na na
vsfGFP-0 (dimer) 485 510 209000 076 159 473
vsfGFP-0 (monomer) 485 510 104500 070 73 218
WasCFP 492 505 51000 085 43 129
WiPhy (Wi-Phy) 700 722 118000 006 7 021
WiPhy2 (Wi-Phy2) 696 719 118000 009 10 031
YPet 517 530 104000 077 80 238
zFP506 Zoanthus sp 496 506 35600 063 22 067
zFP538 Zoanthus sp 528 538 20200 042 8 025
Table ignores photostability (photoinstability) molecular weight monomer vs dimer vs tetramer oligomerization
aggregation prone-ness pKa (pH sensitivity) and more Jellyfish (EGFP) coral Amphioxus etc fluorescent proteins
require one or two oxygen molecules to manufacture the chromophore bonds (a product is H2O2) LOV proteins require
Flavin cofactor Wi-Phy iRFPs UnaG require biliverdin This table also excludes photocontrollable fluorescent proteins
photoactivatable photoconvertible photoswitchable Cranfill et al (2016) published an experimental direct comparison of
several popular fluorescent proteins with literature comparisons NTnC GECI (genetically engineered Ca++ ion indicator)
is used as an example of a small state of the art fluorescent protein biosensor also shown is Ca++ ion insensitive double
mutant control (Barykina et al 2016) NTnC is an inverse signal (No Ca++ bright Ca++ saturated ~50 brightness) FP
biosensor ndash the paper shows simultaneous use with R-GECO (red fluorescence dark to bright) FP biosensor ndash these
could be used together to enhance ratiometric response Subcellular localization ex to cytoplasmic side of plasma
membrane could be used to further improve signal-to-noise ratio and provide compartment specific data The NTnC
Ca++ insensitive double mutant and mCherry (R-GECOrsquos parent Zhao et al 2011) could be simultaneously localized and
concentrated to the nucleolus Golgi body or other lsquoout of the wayrsquo compartment PubMed
(httpswwwncbinlmnihgovpubmed) Google ScienceDirect Highwire Press and other search engines and publishers
can be used to find full text publications of all fluorophores and fluorescent proteins listed Photo-changeable fluorescent
proteins are reviewed in Turkowyd et al (2016) and summarized in Lambert T Thorn (2016) SandercyaninBLA is a
lipocalin family member non-fluorescent apo-protein is 18 kDa fluorescent tetramer is 75 kDa quantum yield in 70
D2O is 166x that of in H2O (00267 vs 0016) (Ghosh et al (2016)
From the SelectedWorks of George McNamara
Fall October 3 2016
McNamara 20161006 revised draft tables for CPHG Light Microscopy and Image Analysispdf
tmp2O7op4pdf
Table 441 Useful Light Microscopy Web Sites
Light Microscopy (overview) Confocal Listserv (not just for confocal microscopes) httpslistsumneducgi-binwaA0=confocalmicroscopy G McNamara ndash Multi-Probe Microscopy (content last updated 2005 includes extensive glossary) httpsworksbepresscomgmcnamara2 Molecular Expressions National High Magnetic Field Laboratory (NHMFL) Florida State University httpmicromagnetfsuedu Microscopedia ndash Resources Guide To Light Microscopy httpwwwmicroscopediacomMicroscopyLight Molecular Probes Inc Invitrogen Thermo Fisher (Many links to useful and informative sites) httpswwwthermofishercomusenhomebrandsmolecular-probeshtml Photonics buyerrsquos guide (much more comprehensive than this list) httpwwwphotonicscomBuyersGuideaspx Science Exchange ndash Reproducibility Initiative (focus on commercial antibodies and uses) httpswwwscienceexchangecomapplicationsreproducibility Wikipedia - Chromosomes httpsenwikipediaorgwikiChromosome Wikipedia ndash Microscope httpenwikipediaorgwikiMicroscope Societies Association of Biomolecular Resource Facilities Light Microscopy Research Group (ABRF LMRG) httpsabrforgresearch-grouplight-microscopy-research-group-lmrg European Microscopy Society (EMS) httpwwweurmicsocorg International Society for Advancement of Cytometry (ISAC) httpisac-netorg Microscopy Society of America (MSA) httpwwwmicroscopyorg Royal Microscopical Society (RMS) httpwwwrmsorguk Major Microscope Companies Leica Microsystems Inc httpwwwleica-microsystemscom httpwwwleica-microsystemscomscience-lab httpwwwleica-microsystemscomproductsconfocal-microscopesdetailsproductleica-tcs-sp8-sted-3x
Karyotype Systems and Digital Pathology Digital Pathology Blog ndash see educational sponsors for comprehensive list of digital pathology vendors httptissuepathologycom 3DHistech ndash digital pathology Pannoramic scanners httpwww3dhistechcom Agilent Pathology Solutions Dako ndash IHC and ISH reagents autostainers httpwwwdakocomdkindexknowledgecenteromnishtm httpwwwdakocomdkar42productgroupshtm Applied Spectral Imaging ndash SKY Spectral karyotyping GenASIs digital pathology httpwwwspectral-imagingcom httpwwwspectral-imagingcomapplicationsspectral-imaging httpwwwspectral-imagingcomapplicationspathology Bioview ndash pathology and FISH cytogenetics scanners httpbioviewcom Definiens (MedImmuneAstraZeneca) - digital pathology image analysis (with deep learning algorithms) httpwwwdefinienscom Huron Digital Pathology ndash large area confocal scanning (entire human brain slices) httpwwwhurondigitalpathologycom Leica Biosystems ndash CytoVision CytogeneticsFISH Aperio digital pathology httpswwwleicabiosystemscomclinical-microscopy-surgery-radiologycytogenetics httpswwwleicabiosystemscomdigital-pathology MetaSystems International ndash karyotyping FISH digital pathology httpsmetasystems-internationalcomenproducts Molecular Probes ThermoFisher Scientific ndash Evos FL Auto and Evos Floid cell imaging stations high content screening (HCS) (Cellomics) httpswwwthermofishercomusenhomelife-sciencecell-analysiscellular-imagingcell-imaging-systemsevos-fl-autohtml httpswwwthermofishercomusenhomelife-sciencecell-analysiscellular-imagingcell-imaging-systemsfloid-cell-imaging-stationhtml httpswwwthermofishercomusenhomelife-sciencecell-analysiscellular-imaginghigh-content-screeninghigh-content-screening-instrumentshtml Philips ndash digital pathology httpwwwusaphilipscomhealthcaresolutionspathology Ventana Roche Digital Pathology ndash digital pathology FISH httpwwwventanacomdigitalpathology Visiopharm ndash digital pathology image analysis (with deep learning algorithms) stereology httpwwwvisiopharmcom
Live cell imaging dishes and multi-well plates (ldquoglass bottom dishesrdquo)
We recommend these for simplified handing and processing vs either thick plastic dishesplates or having to handle loose coverslips with your valuable cells
Leicester Imaging Technologies (LITE) - University of Leicester - table of dishes for live cell imaging (our table has current links and bad links left out) httpwww2leacukcollegesmedbiopsychfacilities-and-servicescbsliteaiftips-and-tricks-1dishes-for-live-cell-imaging Bellco Glass Inc - glass flat bottom plate httpwwwbellcoglasscomnode1046 Bioptechs - interchangable coverslip dish ($036 per experiment) httpwwwbioptechscomproductinterchangeable-coverslip-dish Brooks Automation Inc - clar bottom optical imaging microplates httpwwwbrookscomproductslife-scienceconsumablesmatrical-microplates-microtubes-racks-lidsglass-bottom-optical-imaging-microplates Cell EampG - glass bottom cell sulture dishes plates httpcellegcomproducthtml Cellvis - glass bottom dishes and plates httpwwwcellviscom_35-mm-glass-bottom-dishes_products_by_categoryphpcat_id=3 Corning - glass bottom microplates httpcatalog2corningcomLifeSciencesen-USShoppingCategoryaspxcategoryname=Microplates20-202015(Lifesciences)|Assay20Microplates20-202015(Lifesciences) Design 1 Solutions - Custom microplates and fabrication services httpwwwdesign1solutionscomcustom-microplateshtml Eppendorf - cell imaging dishes imaging plates httpsonline-shopeppendorfcomOC-enCell-Culture-and-Imaging-Consumables-110320Cell-Imaging-Consumables-120210Eppendorf-Cell-Imaging-Dishes-PF-19457html httpsonline-shopeppendorfcomOC-enCell-Culture-and-Imaging-Consumables-110320Cell-Imaging-Consumables-120210Eppendorf-Cell-Imaging-Plates-PF-19458html Greiner Bio-One International - CellView glass bottom dishes quadrant dishes httpsshopgbocomenrowarticlescataloguearticle0110_0110_0040_002027513 ibidi - u-Plates glass bottom dishes microfluidic slides httpibidicomxtproductsenibidi-Labwarem-Plates httpibidicomxtproductsenibidi-Labware Matsunami Glass Ind Ltd httpwwwmatsunami-glasscojpenglishlifebiodata01html Mattek - glass bottom dishes and SBS plates httpswwwmattekcomstore-categoryculturewareglass-bottom-dishes Nunc - glass bottom dishes httpswwwthermofishercomordercatalogproduct150680 NEST Biotechnology Co Ltd Wuxi Jiangsu PRChina httpwwwnestscientificusacomproducts_detailproductId=31html PerkinElmer - glass bottom plates httpwwwperkinelmercomSECatalogCategoryIDMicroplates
Porvair Sciences - clear bottom assay plates httpwwwporvair-sciencescomenservices-menulife-sciencesclear-bottom-assay-plates Ted Pella Inc - glass bottom dishes clear wall or black wall (manufactured by WillCo Wells) httpswwwtedpellacomsection_html706dishhtm Warner Instruments (Harvard Apparatus) - culture dishes perfusion systems httpswwwwarneronlinecomproduct_cat_infocfmid=1322 Willco Wells - glass bottom dishes wwwwillcowellscom World Precision Instruments - Fluorodish glass bottom dishes httpswwwwpiinccomproductslaboratory-suppliesfd35-100-fluorodish-cell-culture-dish-35mm-23mm-well-pkg-of-100 Microscopes Microscopy Accessories (accessorize) Reagents Resources 3scan ndash knife edge scanning microscope (images slices repeats through specimen) httpwww3scancomdata-2 89North (affiliated with Chroma Technology Corp) httpwww89northcom Abberior Instruments ndash STED RESOLFT precision localization super-resolution microscopes STEDYCON convert any fluorescence microscope to STED httpabberior-instrumentscom httpabberior-instrumentscomproductscompact-lineabberior-stedy-con Acquifer ndash HIVE imaging machine (computer hardware) for high content screening big data httpswwwacquiferde Adobe Systems Inc httpwwwAdobecom httpscreativeadobecomproductsdownloadphotoshop Affymetrix eBioscience (ThermoFisher) - microarrays httpwwwaffymetrixcomestorebrowselevel_one_category_template_onejspparent=35796ampcategory=35796 httpwwwaffymetrixcomestorecatalog131547AFFYGeneChip26231743B+System+3000Dx+v22623423B1_1 Agilent Technologies ndash SureScan microarray scanner (array CGH microRNA SNPs CNV) httpwwwgenomicsagilentcomenMicroarray-Scanner-Processing-HardwareSureScan-Microarray-Scannercid=AG-PT-144 Andor Technology (Oxford Instruments) ndash sCMOS cameras Dragonfly spinning disk confocal Revolution DSD2 differential spinning disk confocal photostimulation (fluorescence recovery after photobleaching optogenetics) Mosaic3 DMDDLP high speed light patterning (optogenetics structured illumination) MicroPoint pulsed UV laser for ablation DNA damage photobleachingactivation uncaging accessories httpwwwandorcomscientific-camerasneo-and-zyla-scmos-cameras httpwwwandorcommicroscopy-systemsdragonfly httpwwwandorcommicroscopy-systemsrevolution-dsd httpwwwandorcommicroscopy-systemsphotostimulation httpwwwandorcommicroscopy-systemsphotostimulationmosaic httpwwwandorcommicroscopy-systemsphotostimulationmicropoint httpwwwandorcommicroscopy-systemsmicroscopy-components Apogee Instruments Inc (Andor Technology) ndash CCD cameras and filter wheels (mostly marketed for astronomy) httpwwwccdcomandorhtml Applied Scientific Instrumentation Inc ndash light sheet imager (iSPIM diSPIM)
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
Karyotype Systems and Digital Pathology Digital Pathology Blog ndash see educational sponsors for comprehensive list of digital pathology vendors httptissuepathologycom 3DHistech ndash digital pathology Pannoramic scanners httpwww3dhistechcom Agilent Pathology Solutions Dako ndash IHC and ISH reagents autostainers httpwwwdakocomdkindexknowledgecenteromnishtm httpwwwdakocomdkar42productgroupshtm Applied Spectral Imaging ndash SKY Spectral karyotyping GenASIs digital pathology httpwwwspectral-imagingcom httpwwwspectral-imagingcomapplicationsspectral-imaging httpwwwspectral-imagingcomapplicationspathology Bioview ndash pathology and FISH cytogenetics scanners httpbioviewcom Definiens (MedImmuneAstraZeneca) - digital pathology image analysis (with deep learning algorithms) httpwwwdefinienscom Huron Digital Pathology ndash large area confocal scanning (entire human brain slices) httpwwwhurondigitalpathologycom Leica Biosystems ndash CytoVision CytogeneticsFISH Aperio digital pathology httpswwwleicabiosystemscomclinical-microscopy-surgery-radiologycytogenetics httpswwwleicabiosystemscomdigital-pathology MetaSystems International ndash karyotyping FISH digital pathology httpsmetasystems-internationalcomenproducts Molecular Probes ThermoFisher Scientific ndash Evos FL Auto and Evos Floid cell imaging stations high content screening (HCS) (Cellomics) httpswwwthermofishercomusenhomelife-sciencecell-analysiscellular-imagingcell-imaging-systemsevos-fl-autohtml httpswwwthermofishercomusenhomelife-sciencecell-analysiscellular-imagingcell-imaging-systemsfloid-cell-imaging-stationhtml httpswwwthermofishercomusenhomelife-sciencecell-analysiscellular-imaginghigh-content-screeninghigh-content-screening-instrumentshtml Philips ndash digital pathology httpwwwusaphilipscomhealthcaresolutionspathology Ventana Roche Digital Pathology ndash digital pathology FISH httpwwwventanacomdigitalpathology Visiopharm ndash digital pathology image analysis (with deep learning algorithms) stereology httpwwwvisiopharmcom
Live cell imaging dishes and multi-well plates (ldquoglass bottom dishesrdquo)
We recommend these for simplified handing and processing vs either thick plastic dishesplates or having to handle loose coverslips with your valuable cells
Leicester Imaging Technologies (LITE) - University of Leicester - table of dishes for live cell imaging (our table has current links and bad links left out) httpwww2leacukcollegesmedbiopsychfacilities-and-servicescbsliteaiftips-and-tricks-1dishes-for-live-cell-imaging Bellco Glass Inc - glass flat bottom plate httpwwwbellcoglasscomnode1046 Bioptechs - interchangable coverslip dish ($036 per experiment) httpwwwbioptechscomproductinterchangeable-coverslip-dish Brooks Automation Inc - clar bottom optical imaging microplates httpwwwbrookscomproductslife-scienceconsumablesmatrical-microplates-microtubes-racks-lidsglass-bottom-optical-imaging-microplates Cell EampG - glass bottom cell sulture dishes plates httpcellegcomproducthtml Cellvis - glass bottom dishes and plates httpwwwcellviscom_35-mm-glass-bottom-dishes_products_by_categoryphpcat_id=3 Corning - glass bottom microplates httpcatalog2corningcomLifeSciencesen-USShoppingCategoryaspxcategoryname=Microplates20-202015(Lifesciences)|Assay20Microplates20-202015(Lifesciences) Design 1 Solutions - Custom microplates and fabrication services httpwwwdesign1solutionscomcustom-microplateshtml Eppendorf - cell imaging dishes imaging plates httpsonline-shopeppendorfcomOC-enCell-Culture-and-Imaging-Consumables-110320Cell-Imaging-Consumables-120210Eppendorf-Cell-Imaging-Dishes-PF-19457html httpsonline-shopeppendorfcomOC-enCell-Culture-and-Imaging-Consumables-110320Cell-Imaging-Consumables-120210Eppendorf-Cell-Imaging-Plates-PF-19458html Greiner Bio-One International - CellView glass bottom dishes quadrant dishes httpsshopgbocomenrowarticlescataloguearticle0110_0110_0040_002027513 ibidi - u-Plates glass bottom dishes microfluidic slides httpibidicomxtproductsenibidi-Labwarem-Plates httpibidicomxtproductsenibidi-Labware Matsunami Glass Ind Ltd httpwwwmatsunami-glasscojpenglishlifebiodata01html Mattek - glass bottom dishes and SBS plates httpswwwmattekcomstore-categoryculturewareglass-bottom-dishes Nunc - glass bottom dishes httpswwwthermofishercomordercatalogproduct150680 NEST Biotechnology Co Ltd Wuxi Jiangsu PRChina httpwwwnestscientificusacomproducts_detailproductId=31html PerkinElmer - glass bottom plates httpwwwperkinelmercomSECatalogCategoryIDMicroplates
Porvair Sciences - clear bottom assay plates httpwwwporvair-sciencescomenservices-menulife-sciencesclear-bottom-assay-plates Ted Pella Inc - glass bottom dishes clear wall or black wall (manufactured by WillCo Wells) httpswwwtedpellacomsection_html706dishhtm Warner Instruments (Harvard Apparatus) - culture dishes perfusion systems httpswwwwarneronlinecomproduct_cat_infocfmid=1322 Willco Wells - glass bottom dishes wwwwillcowellscom World Precision Instruments - Fluorodish glass bottom dishes httpswwwwpiinccomproductslaboratory-suppliesfd35-100-fluorodish-cell-culture-dish-35mm-23mm-well-pkg-of-100 Microscopes Microscopy Accessories (accessorize) Reagents Resources 3scan ndash knife edge scanning microscope (images slices repeats through specimen) httpwww3scancomdata-2 89North (affiliated with Chroma Technology Corp) httpwww89northcom Abberior Instruments ndash STED RESOLFT precision localization super-resolution microscopes STEDYCON convert any fluorescence microscope to STED httpabberior-instrumentscom httpabberior-instrumentscomproductscompact-lineabberior-stedy-con Acquifer ndash HIVE imaging machine (computer hardware) for high content screening big data httpswwwacquiferde Adobe Systems Inc httpwwwAdobecom httpscreativeadobecomproductsdownloadphotoshop Affymetrix eBioscience (ThermoFisher) - microarrays httpwwwaffymetrixcomestorebrowselevel_one_category_template_onejspparent=35796ampcategory=35796 httpwwwaffymetrixcomestorecatalog131547AFFYGeneChip26231743B+System+3000Dx+v22623423B1_1 Agilent Technologies ndash SureScan microarray scanner (array CGH microRNA SNPs CNV) httpwwwgenomicsagilentcomenMicroarray-Scanner-Processing-HardwareSureScan-Microarray-Scannercid=AG-PT-144 Andor Technology (Oxford Instruments) ndash sCMOS cameras Dragonfly spinning disk confocal Revolution DSD2 differential spinning disk confocal photostimulation (fluorescence recovery after photobleaching optogenetics) Mosaic3 DMDDLP high speed light patterning (optogenetics structured illumination) MicroPoint pulsed UV laser for ablation DNA damage photobleachingactivation uncaging accessories httpwwwandorcomscientific-camerasneo-and-zyla-scmos-cameras httpwwwandorcommicroscopy-systemsdragonfly httpwwwandorcommicroscopy-systemsrevolution-dsd httpwwwandorcommicroscopy-systemsphotostimulation httpwwwandorcommicroscopy-systemsphotostimulationmosaic httpwwwandorcommicroscopy-systemsphotostimulationmicropoint httpwwwandorcommicroscopy-systemsmicroscopy-components Apogee Instruments Inc (Andor Technology) ndash CCD cameras and filter wheels (mostly marketed for astronomy) httpwwwccdcomandorhtml Applied Scientific Instrumentation Inc ndash light sheet imager (iSPIM diSPIM)
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
Live cell imaging dishes and multi-well plates (ldquoglass bottom dishesrdquo)
We recommend these for simplified handing and processing vs either thick plastic dishesplates or having to handle loose coverslips with your valuable cells
Leicester Imaging Technologies (LITE) - University of Leicester - table of dishes for live cell imaging (our table has current links and bad links left out) httpwww2leacukcollegesmedbiopsychfacilities-and-servicescbsliteaiftips-and-tricks-1dishes-for-live-cell-imaging Bellco Glass Inc - glass flat bottom plate httpwwwbellcoglasscomnode1046 Bioptechs - interchangable coverslip dish ($036 per experiment) httpwwwbioptechscomproductinterchangeable-coverslip-dish Brooks Automation Inc - clar bottom optical imaging microplates httpwwwbrookscomproductslife-scienceconsumablesmatrical-microplates-microtubes-racks-lidsglass-bottom-optical-imaging-microplates Cell EampG - glass bottom cell sulture dishes plates httpcellegcomproducthtml Cellvis - glass bottom dishes and plates httpwwwcellviscom_35-mm-glass-bottom-dishes_products_by_categoryphpcat_id=3 Corning - glass bottom microplates httpcatalog2corningcomLifeSciencesen-USShoppingCategoryaspxcategoryname=Microplates20-202015(Lifesciences)|Assay20Microplates20-202015(Lifesciences) Design 1 Solutions - Custom microplates and fabrication services httpwwwdesign1solutionscomcustom-microplateshtml Eppendorf - cell imaging dishes imaging plates httpsonline-shopeppendorfcomOC-enCell-Culture-and-Imaging-Consumables-110320Cell-Imaging-Consumables-120210Eppendorf-Cell-Imaging-Dishes-PF-19457html httpsonline-shopeppendorfcomOC-enCell-Culture-and-Imaging-Consumables-110320Cell-Imaging-Consumables-120210Eppendorf-Cell-Imaging-Plates-PF-19458html Greiner Bio-One International - CellView glass bottom dishes quadrant dishes httpsshopgbocomenrowarticlescataloguearticle0110_0110_0040_002027513 ibidi - u-Plates glass bottom dishes microfluidic slides httpibidicomxtproductsenibidi-Labwarem-Plates httpibidicomxtproductsenibidi-Labware Matsunami Glass Ind Ltd httpwwwmatsunami-glasscojpenglishlifebiodata01html Mattek - glass bottom dishes and SBS plates httpswwwmattekcomstore-categoryculturewareglass-bottom-dishes Nunc - glass bottom dishes httpswwwthermofishercomordercatalogproduct150680 NEST Biotechnology Co Ltd Wuxi Jiangsu PRChina httpwwwnestscientificusacomproducts_detailproductId=31html PerkinElmer - glass bottom plates httpwwwperkinelmercomSECatalogCategoryIDMicroplates
Porvair Sciences - clear bottom assay plates httpwwwporvair-sciencescomenservices-menulife-sciencesclear-bottom-assay-plates Ted Pella Inc - glass bottom dishes clear wall or black wall (manufactured by WillCo Wells) httpswwwtedpellacomsection_html706dishhtm Warner Instruments (Harvard Apparatus) - culture dishes perfusion systems httpswwwwarneronlinecomproduct_cat_infocfmid=1322 Willco Wells - glass bottom dishes wwwwillcowellscom World Precision Instruments - Fluorodish glass bottom dishes httpswwwwpiinccomproductslaboratory-suppliesfd35-100-fluorodish-cell-culture-dish-35mm-23mm-well-pkg-of-100 Microscopes Microscopy Accessories (accessorize) Reagents Resources 3scan ndash knife edge scanning microscope (images slices repeats through specimen) httpwww3scancomdata-2 89North (affiliated with Chroma Technology Corp) httpwww89northcom Abberior Instruments ndash STED RESOLFT precision localization super-resolution microscopes STEDYCON convert any fluorescence microscope to STED httpabberior-instrumentscom httpabberior-instrumentscomproductscompact-lineabberior-stedy-con Acquifer ndash HIVE imaging machine (computer hardware) for high content screening big data httpswwwacquiferde Adobe Systems Inc httpwwwAdobecom httpscreativeadobecomproductsdownloadphotoshop Affymetrix eBioscience (ThermoFisher) - microarrays httpwwwaffymetrixcomestorebrowselevel_one_category_template_onejspparent=35796ampcategory=35796 httpwwwaffymetrixcomestorecatalog131547AFFYGeneChip26231743B+System+3000Dx+v22623423B1_1 Agilent Technologies ndash SureScan microarray scanner (array CGH microRNA SNPs CNV) httpwwwgenomicsagilentcomenMicroarray-Scanner-Processing-HardwareSureScan-Microarray-Scannercid=AG-PT-144 Andor Technology (Oxford Instruments) ndash sCMOS cameras Dragonfly spinning disk confocal Revolution DSD2 differential spinning disk confocal photostimulation (fluorescence recovery after photobleaching optogenetics) Mosaic3 DMDDLP high speed light patterning (optogenetics structured illumination) MicroPoint pulsed UV laser for ablation DNA damage photobleachingactivation uncaging accessories httpwwwandorcomscientific-camerasneo-and-zyla-scmos-cameras httpwwwandorcommicroscopy-systemsdragonfly httpwwwandorcommicroscopy-systemsrevolution-dsd httpwwwandorcommicroscopy-systemsphotostimulation httpwwwandorcommicroscopy-systemsphotostimulationmosaic httpwwwandorcommicroscopy-systemsphotostimulationmicropoint httpwwwandorcommicroscopy-systemsmicroscopy-components Apogee Instruments Inc (Andor Technology) ndash CCD cameras and filter wheels (mostly marketed for astronomy) httpwwwccdcomandorhtml Applied Scientific Instrumentation Inc ndash light sheet imager (iSPIM diSPIM)
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
Live cell imaging dishes and multi-well plates (ldquoglass bottom dishesrdquo)
We recommend these for simplified handing and processing vs either thick plastic dishesplates or having to handle loose coverslips with your valuable cells
Leicester Imaging Technologies (LITE) - University of Leicester - table of dishes for live cell imaging (our table has current links and bad links left out) httpwww2leacukcollegesmedbiopsychfacilities-and-servicescbsliteaiftips-and-tricks-1dishes-for-live-cell-imaging Bellco Glass Inc - glass flat bottom plate httpwwwbellcoglasscomnode1046 Bioptechs - interchangable coverslip dish ($036 per experiment) httpwwwbioptechscomproductinterchangeable-coverslip-dish Brooks Automation Inc - clar bottom optical imaging microplates httpwwwbrookscomproductslife-scienceconsumablesmatrical-microplates-microtubes-racks-lidsglass-bottom-optical-imaging-microplates Cell EampG - glass bottom cell sulture dishes plates httpcellegcomproducthtml Cellvis - glass bottom dishes and plates httpwwwcellviscom_35-mm-glass-bottom-dishes_products_by_categoryphpcat_id=3 Corning - glass bottom microplates httpcatalog2corningcomLifeSciencesen-USShoppingCategoryaspxcategoryname=Microplates20-202015(Lifesciences)|Assay20Microplates20-202015(Lifesciences) Design 1 Solutions - Custom microplates and fabrication services httpwwwdesign1solutionscomcustom-microplateshtml Eppendorf - cell imaging dishes imaging plates httpsonline-shopeppendorfcomOC-enCell-Culture-and-Imaging-Consumables-110320Cell-Imaging-Consumables-120210Eppendorf-Cell-Imaging-Dishes-PF-19457html httpsonline-shopeppendorfcomOC-enCell-Culture-and-Imaging-Consumables-110320Cell-Imaging-Consumables-120210Eppendorf-Cell-Imaging-Plates-PF-19458html Greiner Bio-One International - CellView glass bottom dishes quadrant dishes httpsshopgbocomenrowarticlescataloguearticle0110_0110_0040_002027513 ibidi - u-Plates glass bottom dishes microfluidic slides httpibidicomxtproductsenibidi-Labwarem-Plates httpibidicomxtproductsenibidi-Labware Matsunami Glass Ind Ltd httpwwwmatsunami-glasscojpenglishlifebiodata01html Mattek - glass bottom dishes and SBS plates httpswwwmattekcomstore-categoryculturewareglass-bottom-dishes Nunc - glass bottom dishes httpswwwthermofishercomordercatalogproduct150680 NEST Biotechnology Co Ltd Wuxi Jiangsu PRChina httpwwwnestscientificusacomproducts_detailproductId=31html PerkinElmer - glass bottom plates httpwwwperkinelmercomSECatalogCategoryIDMicroplates
Porvair Sciences - clear bottom assay plates httpwwwporvair-sciencescomenservices-menulife-sciencesclear-bottom-assay-plates Ted Pella Inc - glass bottom dishes clear wall or black wall (manufactured by WillCo Wells) httpswwwtedpellacomsection_html706dishhtm Warner Instruments (Harvard Apparatus) - culture dishes perfusion systems httpswwwwarneronlinecomproduct_cat_infocfmid=1322 Willco Wells - glass bottom dishes wwwwillcowellscom World Precision Instruments - Fluorodish glass bottom dishes httpswwwwpiinccomproductslaboratory-suppliesfd35-100-fluorodish-cell-culture-dish-35mm-23mm-well-pkg-of-100 Microscopes Microscopy Accessories (accessorize) Reagents Resources 3scan ndash knife edge scanning microscope (images slices repeats through specimen) httpwww3scancomdata-2 89North (affiliated with Chroma Technology Corp) httpwww89northcom Abberior Instruments ndash STED RESOLFT precision localization super-resolution microscopes STEDYCON convert any fluorescence microscope to STED httpabberior-instrumentscom httpabberior-instrumentscomproductscompact-lineabberior-stedy-con Acquifer ndash HIVE imaging machine (computer hardware) for high content screening big data httpswwwacquiferde Adobe Systems Inc httpwwwAdobecom httpscreativeadobecomproductsdownloadphotoshop Affymetrix eBioscience (ThermoFisher) - microarrays httpwwwaffymetrixcomestorebrowselevel_one_category_template_onejspparent=35796ampcategory=35796 httpwwwaffymetrixcomestorecatalog131547AFFYGeneChip26231743B+System+3000Dx+v22623423B1_1 Agilent Technologies ndash SureScan microarray scanner (array CGH microRNA SNPs CNV) httpwwwgenomicsagilentcomenMicroarray-Scanner-Processing-HardwareSureScan-Microarray-Scannercid=AG-PT-144 Andor Technology (Oxford Instruments) ndash sCMOS cameras Dragonfly spinning disk confocal Revolution DSD2 differential spinning disk confocal photostimulation (fluorescence recovery after photobleaching optogenetics) Mosaic3 DMDDLP high speed light patterning (optogenetics structured illumination) MicroPoint pulsed UV laser for ablation DNA damage photobleachingactivation uncaging accessories httpwwwandorcomscientific-camerasneo-and-zyla-scmos-cameras httpwwwandorcommicroscopy-systemsdragonfly httpwwwandorcommicroscopy-systemsrevolution-dsd httpwwwandorcommicroscopy-systemsphotostimulation httpwwwandorcommicroscopy-systemsphotostimulationmosaic httpwwwandorcommicroscopy-systemsphotostimulationmicropoint httpwwwandorcommicroscopy-systemsmicroscopy-components Apogee Instruments Inc (Andor Technology) ndash CCD cameras and filter wheels (mostly marketed for astronomy) httpwwwccdcomandorhtml Applied Scientific Instrumentation Inc ndash light sheet imager (iSPIM diSPIM)
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
Live cell imaging dishes and multi-well plates (ldquoglass bottom dishesrdquo)
We recommend these for simplified handing and processing vs either thick plastic dishesplates or having to handle loose coverslips with your valuable cells
Leicester Imaging Technologies (LITE) - University of Leicester - table of dishes for live cell imaging (our table has current links and bad links left out) httpwww2leacukcollegesmedbiopsychfacilities-and-servicescbsliteaiftips-and-tricks-1dishes-for-live-cell-imaging Bellco Glass Inc - glass flat bottom plate httpwwwbellcoglasscomnode1046 Bioptechs - interchangable coverslip dish ($036 per experiment) httpwwwbioptechscomproductinterchangeable-coverslip-dish Brooks Automation Inc - clar bottom optical imaging microplates httpwwwbrookscomproductslife-scienceconsumablesmatrical-microplates-microtubes-racks-lidsglass-bottom-optical-imaging-microplates Cell EampG - glass bottom cell sulture dishes plates httpcellegcomproducthtml Cellvis - glass bottom dishes and plates httpwwwcellviscom_35-mm-glass-bottom-dishes_products_by_categoryphpcat_id=3 Corning - glass bottom microplates httpcatalog2corningcomLifeSciencesen-USShoppingCategoryaspxcategoryname=Microplates20-202015(Lifesciences)|Assay20Microplates20-202015(Lifesciences) Design 1 Solutions - Custom microplates and fabrication services httpwwwdesign1solutionscomcustom-microplateshtml Eppendorf - cell imaging dishes imaging plates httpsonline-shopeppendorfcomOC-enCell-Culture-and-Imaging-Consumables-110320Cell-Imaging-Consumables-120210Eppendorf-Cell-Imaging-Dishes-PF-19457html httpsonline-shopeppendorfcomOC-enCell-Culture-and-Imaging-Consumables-110320Cell-Imaging-Consumables-120210Eppendorf-Cell-Imaging-Plates-PF-19458html Greiner Bio-One International - CellView glass bottom dishes quadrant dishes httpsshopgbocomenrowarticlescataloguearticle0110_0110_0040_002027513 ibidi - u-Plates glass bottom dishes microfluidic slides httpibidicomxtproductsenibidi-Labwarem-Plates httpibidicomxtproductsenibidi-Labware Matsunami Glass Ind Ltd httpwwwmatsunami-glasscojpenglishlifebiodata01html Mattek - glass bottom dishes and SBS plates httpswwwmattekcomstore-categoryculturewareglass-bottom-dishes Nunc - glass bottom dishes httpswwwthermofishercomordercatalogproduct150680 NEST Biotechnology Co Ltd Wuxi Jiangsu PRChina httpwwwnestscientificusacomproducts_detailproductId=31html PerkinElmer - glass bottom plates httpwwwperkinelmercomSECatalogCategoryIDMicroplates
Porvair Sciences - clear bottom assay plates httpwwwporvair-sciencescomenservices-menulife-sciencesclear-bottom-assay-plates Ted Pella Inc - glass bottom dishes clear wall or black wall (manufactured by WillCo Wells) httpswwwtedpellacomsection_html706dishhtm Warner Instruments (Harvard Apparatus) - culture dishes perfusion systems httpswwwwarneronlinecomproduct_cat_infocfmid=1322 Willco Wells - glass bottom dishes wwwwillcowellscom World Precision Instruments - Fluorodish glass bottom dishes httpswwwwpiinccomproductslaboratory-suppliesfd35-100-fluorodish-cell-culture-dish-35mm-23mm-well-pkg-of-100 Microscopes Microscopy Accessories (accessorize) Reagents Resources 3scan ndash knife edge scanning microscope (images slices repeats through specimen) httpwww3scancomdata-2 89North (affiliated with Chroma Technology Corp) httpwww89northcom Abberior Instruments ndash STED RESOLFT precision localization super-resolution microscopes STEDYCON convert any fluorescence microscope to STED httpabberior-instrumentscom httpabberior-instrumentscomproductscompact-lineabberior-stedy-con Acquifer ndash HIVE imaging machine (computer hardware) for high content screening big data httpswwwacquiferde Adobe Systems Inc httpwwwAdobecom httpscreativeadobecomproductsdownloadphotoshop Affymetrix eBioscience (ThermoFisher) - microarrays httpwwwaffymetrixcomestorebrowselevel_one_category_template_onejspparent=35796ampcategory=35796 httpwwwaffymetrixcomestorecatalog131547AFFYGeneChip26231743B+System+3000Dx+v22623423B1_1 Agilent Technologies ndash SureScan microarray scanner (array CGH microRNA SNPs CNV) httpwwwgenomicsagilentcomenMicroarray-Scanner-Processing-HardwareSureScan-Microarray-Scannercid=AG-PT-144 Andor Technology (Oxford Instruments) ndash sCMOS cameras Dragonfly spinning disk confocal Revolution DSD2 differential spinning disk confocal photostimulation (fluorescence recovery after photobleaching optogenetics) Mosaic3 DMDDLP high speed light patterning (optogenetics structured illumination) MicroPoint pulsed UV laser for ablation DNA damage photobleachingactivation uncaging accessories httpwwwandorcomscientific-camerasneo-and-zyla-scmos-cameras httpwwwandorcommicroscopy-systemsdragonfly httpwwwandorcommicroscopy-systemsrevolution-dsd httpwwwandorcommicroscopy-systemsphotostimulation httpwwwandorcommicroscopy-systemsphotostimulationmosaic httpwwwandorcommicroscopy-systemsphotostimulationmicropoint httpwwwandorcommicroscopy-systemsmicroscopy-components Apogee Instruments Inc (Andor Technology) ndash CCD cameras and filter wheels (mostly marketed for astronomy) httpwwwccdcomandorhtml Applied Scientific Instrumentation Inc ndash light sheet imager (iSPIM diSPIM)
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
Live cell imaging dishes and multi-well plates (ldquoglass bottom dishesrdquo)
We recommend these for simplified handing and processing vs either thick plastic dishesplates or having to handle loose coverslips with your valuable cells
Leicester Imaging Technologies (LITE) - University of Leicester - table of dishes for live cell imaging (our table has current links and bad links left out) httpwww2leacukcollegesmedbiopsychfacilities-and-servicescbsliteaiftips-and-tricks-1dishes-for-live-cell-imaging Bellco Glass Inc - glass flat bottom plate httpwwwbellcoglasscomnode1046 Bioptechs - interchangable coverslip dish ($036 per experiment) httpwwwbioptechscomproductinterchangeable-coverslip-dish Brooks Automation Inc - clar bottom optical imaging microplates httpwwwbrookscomproductslife-scienceconsumablesmatrical-microplates-microtubes-racks-lidsglass-bottom-optical-imaging-microplates Cell EampG - glass bottom cell sulture dishes plates httpcellegcomproducthtml Cellvis - glass bottom dishes and plates httpwwwcellviscom_35-mm-glass-bottom-dishes_products_by_categoryphpcat_id=3 Corning - glass bottom microplates httpcatalog2corningcomLifeSciencesen-USShoppingCategoryaspxcategoryname=Microplates20-202015(Lifesciences)|Assay20Microplates20-202015(Lifesciences) Design 1 Solutions - Custom microplates and fabrication services httpwwwdesign1solutionscomcustom-microplateshtml Eppendorf - cell imaging dishes imaging plates httpsonline-shopeppendorfcomOC-enCell-Culture-and-Imaging-Consumables-110320Cell-Imaging-Consumables-120210Eppendorf-Cell-Imaging-Dishes-PF-19457html httpsonline-shopeppendorfcomOC-enCell-Culture-and-Imaging-Consumables-110320Cell-Imaging-Consumables-120210Eppendorf-Cell-Imaging-Plates-PF-19458html Greiner Bio-One International - CellView glass bottom dishes quadrant dishes httpsshopgbocomenrowarticlescataloguearticle0110_0110_0040_002027513 ibidi - u-Plates glass bottom dishes microfluidic slides httpibidicomxtproductsenibidi-Labwarem-Plates httpibidicomxtproductsenibidi-Labware Matsunami Glass Ind Ltd httpwwwmatsunami-glasscojpenglishlifebiodata01html Mattek - glass bottom dishes and SBS plates httpswwwmattekcomstore-categoryculturewareglass-bottom-dishes Nunc - glass bottom dishes httpswwwthermofishercomordercatalogproduct150680 NEST Biotechnology Co Ltd Wuxi Jiangsu PRChina httpwwwnestscientificusacomproducts_detailproductId=31html PerkinElmer - glass bottom plates httpwwwperkinelmercomSECatalogCategoryIDMicroplates
Porvair Sciences - clear bottom assay plates httpwwwporvair-sciencescomenservices-menulife-sciencesclear-bottom-assay-plates Ted Pella Inc - glass bottom dishes clear wall or black wall (manufactured by WillCo Wells) httpswwwtedpellacomsection_html706dishhtm Warner Instruments (Harvard Apparatus) - culture dishes perfusion systems httpswwwwarneronlinecomproduct_cat_infocfmid=1322 Willco Wells - glass bottom dishes wwwwillcowellscom World Precision Instruments - Fluorodish glass bottom dishes httpswwwwpiinccomproductslaboratory-suppliesfd35-100-fluorodish-cell-culture-dish-35mm-23mm-well-pkg-of-100 Microscopes Microscopy Accessories (accessorize) Reagents Resources 3scan ndash knife edge scanning microscope (images slices repeats through specimen) httpwww3scancomdata-2 89North (affiliated with Chroma Technology Corp) httpwww89northcom Abberior Instruments ndash STED RESOLFT precision localization super-resolution microscopes STEDYCON convert any fluorescence microscope to STED httpabberior-instrumentscom httpabberior-instrumentscomproductscompact-lineabberior-stedy-con Acquifer ndash HIVE imaging machine (computer hardware) for high content screening big data httpswwwacquiferde Adobe Systems Inc httpwwwAdobecom httpscreativeadobecomproductsdownloadphotoshop Affymetrix eBioscience (ThermoFisher) - microarrays httpwwwaffymetrixcomestorebrowselevel_one_category_template_onejspparent=35796ampcategory=35796 httpwwwaffymetrixcomestorecatalog131547AFFYGeneChip26231743B+System+3000Dx+v22623423B1_1 Agilent Technologies ndash SureScan microarray scanner (array CGH microRNA SNPs CNV) httpwwwgenomicsagilentcomenMicroarray-Scanner-Processing-HardwareSureScan-Microarray-Scannercid=AG-PT-144 Andor Technology (Oxford Instruments) ndash sCMOS cameras Dragonfly spinning disk confocal Revolution DSD2 differential spinning disk confocal photostimulation (fluorescence recovery after photobleaching optogenetics) Mosaic3 DMDDLP high speed light patterning (optogenetics structured illumination) MicroPoint pulsed UV laser for ablation DNA damage photobleachingactivation uncaging accessories httpwwwandorcomscientific-camerasneo-and-zyla-scmos-cameras httpwwwandorcommicroscopy-systemsdragonfly httpwwwandorcommicroscopy-systemsrevolution-dsd httpwwwandorcommicroscopy-systemsphotostimulation httpwwwandorcommicroscopy-systemsphotostimulationmosaic httpwwwandorcommicroscopy-systemsphotostimulationmicropoint httpwwwandorcommicroscopy-systemsmicroscopy-components Apogee Instruments Inc (Andor Technology) ndash CCD cameras and filter wheels (mostly marketed for astronomy) httpwwwccdcomandorhtml Applied Scientific Instrumentation Inc ndash light sheet imager (iSPIM diSPIM)
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
TGP (Thermal Green Protein) 493 507 64000 066 42 126
Topaz 514 527 94500 06 57 169
UnaG 498 527 77300 051 39 117
VafLOV 450 495 12500 023 3 009
Venus2 (V2) 515 528 184000 057 105 312
Venus3 (V3) 515 528 276000 057 157 468
Venus4 (V4) 515 528 368000 057 210 624
Venus5 (V5) 515 528 460000 057 262 780
Venus6 (V6) 515 528 552000 057 315 936
Violeta (GFPmut3W66) 350 425 na na na na
vsfGFP-0 (dimer) 485 510 209000 076 159 473
vsfGFP-0 (monomer) 485 510 104500 070 73 218
WasCFP 492 505 51000 085 43 129
WiPhy (Wi-Phy) 700 722 118000 006 7 021
WiPhy2 (Wi-Phy2) 696 719 118000 009 10 031
YPet 517 530 104000 077 80 238
zFP506 Zoanthus sp 496 506 35600 063 22 067
zFP538 Zoanthus sp 528 538 20200 042 8 025
Table ignores photostability (photoinstability) molecular weight monomer vs dimer vs tetramer oligomerization
aggregation prone-ness pKa (pH sensitivity) and more Jellyfish (EGFP) coral Amphioxus etc fluorescent proteins
require one or two oxygen molecules to manufacture the chromophore bonds (a product is H2O2) LOV proteins require
Flavin cofactor Wi-Phy iRFPs UnaG require biliverdin This table also excludes photocontrollable fluorescent proteins
photoactivatable photoconvertible photoswitchable Cranfill et al (2016) published an experimental direct comparison of
several popular fluorescent proteins with literature comparisons NTnC GECI (genetically engineered Ca++ ion indicator)
is used as an example of a small state of the art fluorescent protein biosensor also shown is Ca++ ion insensitive double
mutant control (Barykina et al 2016) NTnC is an inverse signal (No Ca++ bright Ca++ saturated ~50 brightness) FP
biosensor ndash the paper shows simultaneous use with R-GECO (red fluorescence dark to bright) FP biosensor ndash these
could be used together to enhance ratiometric response Subcellular localization ex to cytoplasmic side of plasma
membrane could be used to further improve signal-to-noise ratio and provide compartment specific data The NTnC
Ca++ insensitive double mutant and mCherry (R-GECOrsquos parent Zhao et al 2011) could be simultaneously localized and
concentrated to the nucleolus Golgi body or other lsquoout of the wayrsquo compartment PubMed
(httpswwwncbinlmnihgovpubmed) Google ScienceDirect Highwire Press and other search engines and publishers
can be used to find full text publications of all fluorophores and fluorescent proteins listed Photo-changeable fluorescent
proteins are reviewed in Turkowyd et al (2016) and summarized in Lambert T Thorn (2016) SandercyaninBLA is a
lipocalin family member non-fluorescent apo-protein is 18 kDa fluorescent tetramer is 75 kDa quantum yield in 70
D2O is 166x that of in H2O (00267 vs 0016) (Ghosh et al (2016)
From the SelectedWorks of George McNamara
Fall October 3 2016
McNamara 20161006 revised draft tables for CPHG Light Microscopy and Image Analysispdf
tmp2O7op4pdf
Live cell imaging dishes and multi-well plates (ldquoglass bottom dishesrdquo)
We recommend these for simplified handing and processing vs either thick plastic dishesplates or having to handle loose coverslips with your valuable cells
Leicester Imaging Technologies (LITE) - University of Leicester - table of dishes for live cell imaging (our table has current links and bad links left out) httpwww2leacukcollegesmedbiopsychfacilities-and-servicescbsliteaiftips-and-tricks-1dishes-for-live-cell-imaging Bellco Glass Inc - glass flat bottom plate httpwwwbellcoglasscomnode1046 Bioptechs - interchangable coverslip dish ($036 per experiment) httpwwwbioptechscomproductinterchangeable-coverslip-dish Brooks Automation Inc - clar bottom optical imaging microplates httpwwwbrookscomproductslife-scienceconsumablesmatrical-microplates-microtubes-racks-lidsglass-bottom-optical-imaging-microplates Cell EampG - glass bottom cell sulture dishes plates httpcellegcomproducthtml Cellvis - glass bottom dishes and plates httpwwwcellviscom_35-mm-glass-bottom-dishes_products_by_categoryphpcat_id=3 Corning - glass bottom microplates httpcatalog2corningcomLifeSciencesen-USShoppingCategoryaspxcategoryname=Microplates20-202015(Lifesciences)|Assay20Microplates20-202015(Lifesciences) Design 1 Solutions - Custom microplates and fabrication services httpwwwdesign1solutionscomcustom-microplateshtml Eppendorf - cell imaging dishes imaging plates httpsonline-shopeppendorfcomOC-enCell-Culture-and-Imaging-Consumables-110320Cell-Imaging-Consumables-120210Eppendorf-Cell-Imaging-Dishes-PF-19457html httpsonline-shopeppendorfcomOC-enCell-Culture-and-Imaging-Consumables-110320Cell-Imaging-Consumables-120210Eppendorf-Cell-Imaging-Plates-PF-19458html Greiner Bio-One International - CellView glass bottom dishes quadrant dishes httpsshopgbocomenrowarticlescataloguearticle0110_0110_0040_002027513 ibidi - u-Plates glass bottom dishes microfluidic slides httpibidicomxtproductsenibidi-Labwarem-Plates httpibidicomxtproductsenibidi-Labware Matsunami Glass Ind Ltd httpwwwmatsunami-glasscojpenglishlifebiodata01html Mattek - glass bottom dishes and SBS plates httpswwwmattekcomstore-categoryculturewareglass-bottom-dishes Nunc - glass bottom dishes httpswwwthermofishercomordercatalogproduct150680 NEST Biotechnology Co Ltd Wuxi Jiangsu PRChina httpwwwnestscientificusacomproducts_detailproductId=31html PerkinElmer - glass bottom plates httpwwwperkinelmercomSECatalogCategoryIDMicroplates
Porvair Sciences - clear bottom assay plates httpwwwporvair-sciencescomenservices-menulife-sciencesclear-bottom-assay-plates Ted Pella Inc - glass bottom dishes clear wall or black wall (manufactured by WillCo Wells) httpswwwtedpellacomsection_html706dishhtm Warner Instruments (Harvard Apparatus) - culture dishes perfusion systems httpswwwwarneronlinecomproduct_cat_infocfmid=1322 Willco Wells - glass bottom dishes wwwwillcowellscom World Precision Instruments - Fluorodish glass bottom dishes httpswwwwpiinccomproductslaboratory-suppliesfd35-100-fluorodish-cell-culture-dish-35mm-23mm-well-pkg-of-100 Microscopes Microscopy Accessories (accessorize) Reagents Resources 3scan ndash knife edge scanning microscope (images slices repeats through specimen) httpwww3scancomdata-2 89North (affiliated with Chroma Technology Corp) httpwww89northcom Abberior Instruments ndash STED RESOLFT precision localization super-resolution microscopes STEDYCON convert any fluorescence microscope to STED httpabberior-instrumentscom httpabberior-instrumentscomproductscompact-lineabberior-stedy-con Acquifer ndash HIVE imaging machine (computer hardware) for high content screening big data httpswwwacquiferde Adobe Systems Inc httpwwwAdobecom httpscreativeadobecomproductsdownloadphotoshop Affymetrix eBioscience (ThermoFisher) - microarrays httpwwwaffymetrixcomestorebrowselevel_one_category_template_onejspparent=35796ampcategory=35796 httpwwwaffymetrixcomestorecatalog131547AFFYGeneChip26231743B+System+3000Dx+v22623423B1_1 Agilent Technologies ndash SureScan microarray scanner (array CGH microRNA SNPs CNV) httpwwwgenomicsagilentcomenMicroarray-Scanner-Processing-HardwareSureScan-Microarray-Scannercid=AG-PT-144 Andor Technology (Oxford Instruments) ndash sCMOS cameras Dragonfly spinning disk confocal Revolution DSD2 differential spinning disk confocal photostimulation (fluorescence recovery after photobleaching optogenetics) Mosaic3 DMDDLP high speed light patterning (optogenetics structured illumination) MicroPoint pulsed UV laser for ablation DNA damage photobleachingactivation uncaging accessories httpwwwandorcomscientific-camerasneo-and-zyla-scmos-cameras httpwwwandorcommicroscopy-systemsdragonfly httpwwwandorcommicroscopy-systemsrevolution-dsd httpwwwandorcommicroscopy-systemsphotostimulation httpwwwandorcommicroscopy-systemsphotostimulationmosaic httpwwwandorcommicroscopy-systemsphotostimulationmicropoint httpwwwandorcommicroscopy-systemsmicroscopy-components Apogee Instruments Inc (Andor Technology) ndash CCD cameras and filter wheels (mostly marketed for astronomy) httpwwwccdcomandorhtml Applied Scientific Instrumentation Inc ndash light sheet imager (iSPIM diSPIM)
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
PhotoChemCAD 20 (Jonathan S Lindsey) dye spectra database and chemistry calculator NIRvana Sciences httpwwwphotochemcadcom httpnirvanasciencescompage_id=3088 UCSF (Lambert Thorn 2016) - Fluorescent protein properties httpnicucsfeduFPvisualization University of Arizona - Fluorescent Spectra Database httpwwwspectraarizonaedu Semrock - Searchlight httpssearchlightsemrockcom Spectroscopy Ninja ndash links to spectral and fluorescence data Spekwin32 spectroscopy software httpwwweffemm2deinfoinfo_specdatahtml
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
TGP (Thermal Green Protein) 493 507 64000 066 42 126
Topaz 514 527 94500 06 57 169
UnaG 498 527 77300 051 39 117
VafLOV 450 495 12500 023 3 009
Venus2 (V2) 515 528 184000 057 105 312
Venus3 (V3) 515 528 276000 057 157 468
Venus4 (V4) 515 528 368000 057 210 624
Venus5 (V5) 515 528 460000 057 262 780
Venus6 (V6) 515 528 552000 057 315 936
Violeta (GFPmut3W66) 350 425 na na na na
vsfGFP-0 (dimer) 485 510 209000 076 159 473
vsfGFP-0 (monomer) 485 510 104500 070 73 218
WasCFP 492 505 51000 085 43 129
WiPhy (Wi-Phy) 700 722 118000 006 7 021
WiPhy2 (Wi-Phy2) 696 719 118000 009 10 031
YPet 517 530 104000 077 80 238
zFP506 Zoanthus sp 496 506 35600 063 22 067
zFP538 Zoanthus sp 528 538 20200 042 8 025
Table ignores photostability (photoinstability) molecular weight monomer vs dimer vs tetramer oligomerization
aggregation prone-ness pKa (pH sensitivity) and more Jellyfish (EGFP) coral Amphioxus etc fluorescent proteins
require one or two oxygen molecules to manufacture the chromophore bonds (a product is H2O2) LOV proteins require
Flavin cofactor Wi-Phy iRFPs UnaG require biliverdin This table also excludes photocontrollable fluorescent proteins
photoactivatable photoconvertible photoswitchable Cranfill et al (2016) published an experimental direct comparison of
several popular fluorescent proteins with literature comparisons NTnC GECI (genetically engineered Ca++ ion indicator)
is used as an example of a small state of the art fluorescent protein biosensor also shown is Ca++ ion insensitive double
mutant control (Barykina et al 2016) NTnC is an inverse signal (No Ca++ bright Ca++ saturated ~50 brightness) FP
biosensor ndash the paper shows simultaneous use with R-GECO (red fluorescence dark to bright) FP biosensor ndash these
could be used together to enhance ratiometric response Subcellular localization ex to cytoplasmic side of plasma
membrane could be used to further improve signal-to-noise ratio and provide compartment specific data The NTnC
Ca++ insensitive double mutant and mCherry (R-GECOrsquos parent Zhao et al 2011) could be simultaneously localized and
concentrated to the nucleolus Golgi body or other lsquoout of the wayrsquo compartment PubMed
(httpswwwncbinlmnihgovpubmed) Google ScienceDirect Highwire Press and other search engines and publishers
can be used to find full text publications of all fluorophores and fluorescent proteins listed Photo-changeable fluorescent
proteins are reviewed in Turkowyd et al (2016) and summarized in Lambert T Thorn (2016) SandercyaninBLA is a
lipocalin family member non-fluorescent apo-protein is 18 kDa fluorescent tetramer is 75 kDa quantum yield in 70
D2O is 166x that of in H2O (00267 vs 0016) (Ghosh et al (2016)
From the SelectedWorks of George McNamara
Fall October 3 2016
McNamara 20161006 revised draft tables for CPHG Light Microscopy and Image Analysispdf
tmp2O7op4pdf
httpwwweffemm2deindexhtml Zeiss ndash interactive fluorescence dye and filter database overview dyes overview filter sets httpswwwmicro-shopzeisscoms=50479761ad21e5ampl=enampp=usampf=fampa=i httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=d httpswwwmicro-shopzeisscoms=55721483f91174ampl=enampp=usampf=fampa=f Researcher Labs Alby Diaspro ndash IIT httpwwwlambsitindexphppage=People Alice Ting ndash MIT httpwwwtinglaborgresearch Allen Institute for Brain Science httpwwwalleninstituteorgwhat-we-dobrain-science Allen Institute for Cell Science httpwwwalleninstituteorgwhat-we-docell-science Amina Qutub - Rice University - How are human cells designed lab httpsqutublaborgqutub-lab Amy Herr UC Berkeley ndash Single cell Westerns httpherrlabberkeleyedu Amy Palmer ndash UC Boulder httpspalmerlabcoloradoedu Andrew York ndash Calico Life Sciences httpswwwcalicolabscomteam-memberandrew-york Anne Carpenter ndash Broad Institute CellProfiler httpspersonalbroadinstituteorganne Arjun Raj UPenn single molecule RNA FISH quantitative understanding cellular functions httprajlabseasupenneduindexhtml httpwwwpopscicomsciencearticle2013-09arjun-raj Atsuko Miyawaki - Laboratory for Cell Function Dynamics (CFDS) Fucci2 fluorescent proteins cell cycle indicator httpwwwbrainrikenjpenfacultydetails30 httpcfdsbrainrikenjp httpcfdsbrainrikenjpFuccihtml Aydogan Ozcan - UCLA - new imaging and sensing architectures httpinnovateeeuclaeduwelcomehtml httpwwwhhmiorgscientistsaydogan-ozcan Badri Roysam - UH - FARSIGHT Toolkit httpwwweeuhedufacultyroysam httpwwwfarsight-toolkitorgwikiMain_Page Brian J Ford ndash microscopist and popularizer httpsuklinkedincominprofbrianjford Carolyn Larabell ndashNational Center for X-ray Tomography (LBNL) UCSF httpncxtlblgovq=carolyn httpprofilesucsfeducarolynlarabell Clare Waterman ndash NHLBI NIH
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
TGP (Thermal Green Protein) 493 507 64000 066 42 126
Topaz 514 527 94500 06 57 169
UnaG 498 527 77300 051 39 117
VafLOV 450 495 12500 023 3 009
Venus2 (V2) 515 528 184000 057 105 312
Venus3 (V3) 515 528 276000 057 157 468
Venus4 (V4) 515 528 368000 057 210 624
Venus5 (V5) 515 528 460000 057 262 780
Venus6 (V6) 515 528 552000 057 315 936
Violeta (GFPmut3W66) 350 425 na na na na
vsfGFP-0 (dimer) 485 510 209000 076 159 473
vsfGFP-0 (monomer) 485 510 104500 070 73 218
WasCFP 492 505 51000 085 43 129
WiPhy (Wi-Phy) 700 722 118000 006 7 021
WiPhy2 (Wi-Phy2) 696 719 118000 009 10 031
YPet 517 530 104000 077 80 238
zFP506 Zoanthus sp 496 506 35600 063 22 067
zFP538 Zoanthus sp 528 538 20200 042 8 025
Table ignores photostability (photoinstability) molecular weight monomer vs dimer vs tetramer oligomerization
aggregation prone-ness pKa (pH sensitivity) and more Jellyfish (EGFP) coral Amphioxus etc fluorescent proteins
require one or two oxygen molecules to manufacture the chromophore bonds (a product is H2O2) LOV proteins require
Flavin cofactor Wi-Phy iRFPs UnaG require biliverdin This table also excludes photocontrollable fluorescent proteins
photoactivatable photoconvertible photoswitchable Cranfill et al (2016) published an experimental direct comparison of
several popular fluorescent proteins with literature comparisons NTnC GECI (genetically engineered Ca++ ion indicator)
is used as an example of a small state of the art fluorescent protein biosensor also shown is Ca++ ion insensitive double
mutant control (Barykina et al 2016) NTnC is an inverse signal (No Ca++ bright Ca++ saturated ~50 brightness) FP
biosensor ndash the paper shows simultaneous use with R-GECO (red fluorescence dark to bright) FP biosensor ndash these
could be used together to enhance ratiometric response Subcellular localization ex to cytoplasmic side of plasma
membrane could be used to further improve signal-to-noise ratio and provide compartment specific data The NTnC
Ca++ insensitive double mutant and mCherry (R-GECOrsquos parent Zhao et al 2011) could be simultaneously localized and
concentrated to the nucleolus Golgi body or other lsquoout of the wayrsquo compartment PubMed
(httpswwwncbinlmnihgovpubmed) Google ScienceDirect Highwire Press and other search engines and publishers
can be used to find full text publications of all fluorophores and fluorescent proteins listed Photo-changeable fluorescent
proteins are reviewed in Turkowyd et al (2016) and summarized in Lambert T Thorn (2016) SandercyaninBLA is a
lipocalin family member non-fluorescent apo-protein is 18 kDa fluorescent tetramer is 75 kDa quantum yield in 70
D2O is 166x that of in H2O (00267 vs 0016) (Ghosh et al (2016)
From the SelectedWorks of George McNamara
Fall October 3 2016
McNamara 20161006 revised draft tables for CPHG Light Microscopy and Image Analysispdf
tmp2O7op4pdf
httpswwwnhlbinihgovresearchintramuralresearcherspiwaterman-clare Dana Peer - Columbia University - Computational Systems Biology viSNE and more software httpwwwc2b2columbiaedudanapeerlabhtml httpwwwc2b2columbiaedudanapeerlabhtmlcythtml David Mayerich - UH - STIM Laboratory Scalable Tissue Imaging and Modeling httpwwweeuhedufacultymayerich httpstimeeuhedu David Piston WUSTL httpwwwcellbiologywustledufacultytenureddavid-w-piston-phd Diane Lidke and Keith Lidke - University of New Mexico httppathologyunmeduresearchfaculty-laboratoriesthe-lidke-laboratoryindexhtml httppanda3physunmedu~klidke Donna Arndt-Jovin and Thomas Jovin - Max Planck Institute for Biophysical Chemistry Laboratory of Cellular Dynamics httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsDonnaArdndt-JovinResearchAreasAndProjects httpwww3mpibpcmpgdegroupsjovinindexphpResearchGroupsChairmanThomasJovinResearchAreasAndProjects Ed Boyden ndash MIT Media Lab ndash expansion microscopy (ExM) optogenetics httpsyntheticneurobiologyorg httpsyntheticneurobiologyorgprotocols httpsyntheticneurobiologyorgpublications httpwwwtedcomtalksed_boyden_baby_diapers_inspired_this_new_way_to_study_the_brain httpwwwextbiocombuycell Elizabeth Blackburn ndash Salk Institute - Telomeres httpswwwsalkeduscientistelizabeth-blackburn httpsenwikipediaorgwikiElizabeth_Blackburn httpswwwnobelprizeorgnobel_prizesmedicinelaureates2009blackburn-factshtml Elizabeth Hillman - Columbia university - SCAPE (Swept Confocally-Aligned Planar Excitation) high speed 3D microscopy DyCE (dynamic contrast enhancement to image and segment small animal organs live) Neuroimaging amp Neurovascular Coupling httporionbmecolumbiaedu~hillmanInstrumentationhtml httporionbmecolumbiaedu~hillmanSCAPEhtml httporionbmecolumbiaedu~hillmanMolecularhtml httporionbmecolumbiaedu~hillmanBrain_Imaginghtml Eric Betzig ndash UCSF (moving from HHMI Janelia Farm in 2016) httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014betzig-factshtml Eva Sevick-Muraca ndash UT Health Science Center at Houston molecular imaging clinical NIR fluoresceince imaging httpswwwutheduimmprofilehtmid=1005459 httpswwwutheduimmcentersmolecular-imaginghtm Evelin Schrock - Technische Universitaumlt Dresden spectral karyotyping cytogenetics httpswwwresearchgatenetprofileEvelin_Schrock Garry Nolan Stanford University ndash Mass cytometry MIBI-ToF high throughput imaging mass cytometry (42plex) httpwebstanfordedugroupnolanresourceshtml httpwebstanfordedugroupnolantechnologieshtml George McNamara ndash Cell Morphometry (41) Figure 1 right top two panels (55) The Chase (2016 temporal area maps lsquodynamic morphologyrsquo) (76) newsletter series on ldquoThe Chaserdquo (73) direct Vimeo movie of ldquoThe Chaserdquo Tiki_Goddess linkedin 10+plex blog httpsworksbepresscomgmcnamara41 httpsworksbepresscomgmcnamara55 httpsworksbepresscomgmcnamara76
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
TGP (Thermal Green Protein) 493 507 64000 066 42 126
Topaz 514 527 94500 06 57 169
UnaG 498 527 77300 051 39 117
VafLOV 450 495 12500 023 3 009
Venus2 (V2) 515 528 184000 057 105 312
Venus3 (V3) 515 528 276000 057 157 468
Venus4 (V4) 515 528 368000 057 210 624
Venus5 (V5) 515 528 460000 057 262 780
Venus6 (V6) 515 528 552000 057 315 936
Violeta (GFPmut3W66) 350 425 na na na na
vsfGFP-0 (dimer) 485 510 209000 076 159 473
vsfGFP-0 (monomer) 485 510 104500 070 73 218
WasCFP 492 505 51000 085 43 129
WiPhy (Wi-Phy) 700 722 118000 006 7 021
WiPhy2 (Wi-Phy2) 696 719 118000 009 10 031
YPet 517 530 104000 077 80 238
zFP506 Zoanthus sp 496 506 35600 063 22 067
zFP538 Zoanthus sp 528 538 20200 042 8 025
Table ignores photostability (photoinstability) molecular weight monomer vs dimer vs tetramer oligomerization
aggregation prone-ness pKa (pH sensitivity) and more Jellyfish (EGFP) coral Amphioxus etc fluorescent proteins
require one or two oxygen molecules to manufacture the chromophore bonds (a product is H2O2) LOV proteins require
Flavin cofactor Wi-Phy iRFPs UnaG require biliverdin This table also excludes photocontrollable fluorescent proteins
photoactivatable photoconvertible photoswitchable Cranfill et al (2016) published an experimental direct comparison of
several popular fluorescent proteins with literature comparisons NTnC GECI (genetically engineered Ca++ ion indicator)
is used as an example of a small state of the art fluorescent protein biosensor also shown is Ca++ ion insensitive double
mutant control (Barykina et al 2016) NTnC is an inverse signal (No Ca++ bright Ca++ saturated ~50 brightness) FP
biosensor ndash the paper shows simultaneous use with R-GECO (red fluorescence dark to bright) FP biosensor ndash these
could be used together to enhance ratiometric response Subcellular localization ex to cytoplasmic side of plasma
membrane could be used to further improve signal-to-noise ratio and provide compartment specific data The NTnC
Ca++ insensitive double mutant and mCherry (R-GECOrsquos parent Zhao et al 2011) could be simultaneously localized and
concentrated to the nucleolus Golgi body or other lsquoout of the wayrsquo compartment PubMed
(httpswwwncbinlmnihgovpubmed) Google ScienceDirect Highwire Press and other search engines and publishers
can be used to find full text publications of all fluorophores and fluorescent proteins listed Photo-changeable fluorescent
proteins are reviewed in Turkowyd et al (2016) and summarized in Lambert T Thorn (2016) SandercyaninBLA is a
lipocalin family member non-fluorescent apo-protein is 18 kDa fluorescent tetramer is 75 kDa quantum yield in 70
D2O is 166x that of in H2O (00267 vs 0016) (Ghosh et al (2016)
From the SelectedWorks of George McNamara
Fall October 3 2016
McNamara 20161006 revised draft tables for CPHG Light Microscopy and Image Analysispdf
tmp2O7op4pdf
httpsworksbepresscomgmcnamara73 httpsvimeocom175151196 httphomeearthlinknet~tiki_goddess httpswwwlinkedincomingeorgemcnamara httpswwwlinkedincompulsebd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara Gillian Griffiths - University of Cambridge - T-cell immunological synapse httpwwwcimrcamacukresearchprincipal-investigatorsprincipal-investigators-a-hgriffiths Hari Shroff NIBIB NIH httpswwwnibibnihgovlabs-at-nibibsection-high-resolution-optical-imaging-hroi HHMI Janelia Farm Research Campus (JFRC) - Advanced Imaging Center Open Science httpswwwjaneliaorgopen-scienceadvanced-imaging-center-aic httpswwwjaneliaorgopen-science Jeanne Lawrence University of Massachussetts Medical School httpwwwumassmededucellbiolabslawrencepersonnelprincipal-investigator Jennifer Lippincott-Schwartz ndash HHMI Janelia Farm httpswwwjaneliaorgpeoplejennifer-lippincott-schwartz Jin Zhang ndash UCSD httpprofilesucsdedujinzhang Joe DeRisi - UCSF - 3D printable lab stuff httpderisilabucsfeduindexphppage=3D John Russ Chris Russ Fovea Pro plugins for Adobe Photoshop httpwwwdrjohnrusscomindexhtml httpwwwdrjohnrusscombookshtml httpwwwreindeergraphicscomfoveaprotutorialhtml Julie Theriot httpcmgmstanfordedutheriot Karl Deisseroth ndash Stanford University ndash CLARITY optical tissue clearing Optogenetics httpclarityresourcecenterorg httpwebstanfordedugroupdlaboptogeneticsindexhtml Karsten Koumlnig (Koenig) - Saarland University and JenLab GmbH - biophotonics httpwwwbltuni-saarlanddeindex_ehtm httpwwwjenlabde httpwwwthelastescape-filmcom Karsten Rodenacker ndash image measurements httpkaro03bplacednetkaroMeMisc_WWWpdfMicrosoftWord-EQUAPATpdf Kevin Eliceiri ndash UW-Madison Open Source Photonics and Software Laboratory for Optical and Computational Instrumentation (LOCI) wwwlociwiscedu Klaus Kemp ndash Microlife Services ndash diatoms and other prepared microscope slides httpwwwdiatomscouk Kurt Thorn UCSF httpthornlabucsfedu httpsnicucsfedublog httpnicucsfeduFPvisualization httpgigapancomgigapans163370 Lihong Wang - WUSTL - Optical Imaging Laboratory photoacoustic tomography
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
TGP (Thermal Green Protein) 493 507 64000 066 42 126
Topaz 514 527 94500 06 57 169
UnaG 498 527 77300 051 39 117
VafLOV 450 495 12500 023 3 009
Venus2 (V2) 515 528 184000 057 105 312
Venus3 (V3) 515 528 276000 057 157 468
Venus4 (V4) 515 528 368000 057 210 624
Venus5 (V5) 515 528 460000 057 262 780
Venus6 (V6) 515 528 552000 057 315 936
Violeta (GFPmut3W66) 350 425 na na na na
vsfGFP-0 (dimer) 485 510 209000 076 159 473
vsfGFP-0 (monomer) 485 510 104500 070 73 218
WasCFP 492 505 51000 085 43 129
WiPhy (Wi-Phy) 700 722 118000 006 7 021
WiPhy2 (Wi-Phy2) 696 719 118000 009 10 031
YPet 517 530 104000 077 80 238
zFP506 Zoanthus sp 496 506 35600 063 22 067
zFP538 Zoanthus sp 528 538 20200 042 8 025
Table ignores photostability (photoinstability) molecular weight monomer vs dimer vs tetramer oligomerization
aggregation prone-ness pKa (pH sensitivity) and more Jellyfish (EGFP) coral Amphioxus etc fluorescent proteins
require one or two oxygen molecules to manufacture the chromophore bonds (a product is H2O2) LOV proteins require
Flavin cofactor Wi-Phy iRFPs UnaG require biliverdin This table also excludes photocontrollable fluorescent proteins
photoactivatable photoconvertible photoswitchable Cranfill et al (2016) published an experimental direct comparison of
several popular fluorescent proteins with literature comparisons NTnC GECI (genetically engineered Ca++ ion indicator)
is used as an example of a small state of the art fluorescent protein biosensor also shown is Ca++ ion insensitive double
mutant control (Barykina et al 2016) NTnC is an inverse signal (No Ca++ bright Ca++ saturated ~50 brightness) FP
biosensor ndash the paper shows simultaneous use with R-GECO (red fluorescence dark to bright) FP biosensor ndash these
could be used together to enhance ratiometric response Subcellular localization ex to cytoplasmic side of plasma
membrane could be used to further improve signal-to-noise ratio and provide compartment specific data The NTnC
Ca++ insensitive double mutant and mCherry (R-GECOrsquos parent Zhao et al 2011) could be simultaneously localized and
concentrated to the nucleolus Golgi body or other lsquoout of the wayrsquo compartment PubMed
(httpswwwncbinlmnihgovpubmed) Google ScienceDirect Highwire Press and other search engines and publishers
can be used to find full text publications of all fluorophores and fluorescent proteins listed Photo-changeable fluorescent
proteins are reviewed in Turkowyd et al (2016) and summarized in Lambert T Thorn (2016) SandercyaninBLA is a
lipocalin family member non-fluorescent apo-protein is 18 kDa fluorescent tetramer is 75 kDa quantum yield in 70
D2O is 166x that of in H2O (00267 vs 0016) (Ghosh et al (2016)
From the SelectedWorks of George McNamara
Fall October 3 2016
McNamara 20161006 revised draft tables for CPHG Light Microscopy and Image Analysispdf
tmp2O7op4pdf
httpoilabseaswustledu httpoilabseaswustleduresearch_0html Loren Looger - HHMI JFRC - iGluSnFr fluorescent protein biosensor httpswwwjaneliaorglablooger-lab Mary-Lou Pardue ndash MIT (first ever in situ hybridization with J Gall) httpsbiologymitedupeoplemary_lou_pardue httpsenwikipediaorgwikiMary-Lou_Pardue Nancy Allbritton - UNC - Chemical cytometry microraft arrays organ-on-a-chip httpswwwbmencsueduindexphpcomponentcomprofileruserprofilenlallbri httpwwwbmeuncedulabsallbritton Navin Varadarajan - UH - single-cell Lab httpwwwcheeuhedufacultyvaradarajan httpsinglecellcheeuheducontacthtml Nicholas Shaner - Scintillon Institute - mNeonGreen httpwwwscintillonorgshaner_lab Rebecca Richards-Kortum httpbioengineeringriceedufacultyrebecca_richards-kortumaspx Robert Campbell - Fluorescent proteins optogenetic proteins httpcampbellwebchemualbertaca httpcampbellwebchemualbertacaprojects-2 Robert Murphy ndash Cell measurements web services httpmurphylabwebcmueduservices Sabine Mai - University of Manitoba - Genomic Centre for Cancer Research and Diagnosis (GCCRD) - httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBPlatformsGCCRDhtml httpswwwumanitobacainstitutesmanitoba_institute_cell_biologyMICBScientistsMaiAwardshtml Sally Ward and Raimund Ober - Texas AampM University - httpwwwwardoberlabcom Simon Watkins - U Pittsburgh Center for Biologic Imaging httpwwwcbppittedufacultywatkinshtml httpwwwcbppittedufacultylabwatkinshtml httpwwwcbipittedu Stefan Hell ndash Deparment Hell NanoBiopohotonics httpwwwmpibpcmpgdehell httpsenwikipediaorgwikiStefan_Hell httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014hell-factshtml Sunney Xie Harvard httpsbernsteinharvardedu Susan Cox ndash 3B microscopy httpwwwcoxphysicscomindexhtml httpwwwcoxphysicscom3b Radim Chmeliacutek - Center for innovative microscopy Experimental biophotonics group httpswwwceiteceuceitec-butexperimental-biophotonicsrg3 httpwwwbiophotczhome Taekjip Ha ndash Johns Hopkins University ndash single molecules httphamedjhmiedu
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
TGP (Thermal Green Protein) 493 507 64000 066 42 126
Topaz 514 527 94500 06 57 169
UnaG 498 527 77300 051 39 117
VafLOV 450 495 12500 023 3 009
Venus2 (V2) 515 528 184000 057 105 312
Venus3 (V3) 515 528 276000 057 157 468
Venus4 (V4) 515 528 368000 057 210 624
Venus5 (V5) 515 528 460000 057 262 780
Venus6 (V6) 515 528 552000 057 315 936
Violeta (GFPmut3W66) 350 425 na na na na
vsfGFP-0 (dimer) 485 510 209000 076 159 473
vsfGFP-0 (monomer) 485 510 104500 070 73 218
WasCFP 492 505 51000 085 43 129
WiPhy (Wi-Phy) 700 722 118000 006 7 021
WiPhy2 (Wi-Phy2) 696 719 118000 009 10 031
YPet 517 530 104000 077 80 238
zFP506 Zoanthus sp 496 506 35600 063 22 067
zFP538 Zoanthus sp 528 538 20200 042 8 025
Table ignores photostability (photoinstability) molecular weight monomer vs dimer vs tetramer oligomerization
aggregation prone-ness pKa (pH sensitivity) and more Jellyfish (EGFP) coral Amphioxus etc fluorescent proteins
require one or two oxygen molecules to manufacture the chromophore bonds (a product is H2O2) LOV proteins require
Flavin cofactor Wi-Phy iRFPs UnaG require biliverdin This table also excludes photocontrollable fluorescent proteins
photoactivatable photoconvertible photoswitchable Cranfill et al (2016) published an experimental direct comparison of
several popular fluorescent proteins with literature comparisons NTnC GECI (genetically engineered Ca++ ion indicator)
is used as an example of a small state of the art fluorescent protein biosensor also shown is Ca++ ion insensitive double
mutant control (Barykina et al 2016) NTnC is an inverse signal (No Ca++ bright Ca++ saturated ~50 brightness) FP
biosensor ndash the paper shows simultaneous use with R-GECO (red fluorescence dark to bright) FP biosensor ndash these
could be used together to enhance ratiometric response Subcellular localization ex to cytoplasmic side of plasma
membrane could be used to further improve signal-to-noise ratio and provide compartment specific data The NTnC
Ca++ insensitive double mutant and mCherry (R-GECOrsquos parent Zhao et al 2011) could be simultaneously localized and
concentrated to the nucleolus Golgi body or other lsquoout of the wayrsquo compartment PubMed
(httpswwwncbinlmnihgovpubmed) Google ScienceDirect Highwire Press and other search engines and publishers
can be used to find full text publications of all fluorophores and fluorescent proteins listed Photo-changeable fluorescent
proteins are reviewed in Turkowyd et al (2016) and summarized in Lambert T Thorn (2016) SandercyaninBLA is a
lipocalin family member non-fluorescent apo-protein is 18 kDa fluorescent tetramer is 75 kDa quantum yield in 70
D2O is 166x that of in H2O (00267 vs 0016) (Ghosh et al (2016)
From the SelectedWorks of George McNamara
Fall October 3 2016
McNamara 20161006 revised draft tables for CPHG Light Microscopy and Image Analysispdf
tmp2O7op4pdf
Thomas Ried ndash NCI NIH SKYM-FISH and CGH Database httpsccrcancergovGenetics-Branchthomas-ried httpsccrcancergovGenetics-Branchthomas-riedqt-staff_profile_tabs=9qt-staff_profile_tabs ftpftpncbinlmnihgovsky-cghDATA httpswwwncbinlmnihgovdbvar Viviana Gradinaru ndash CalTech ndash CLARITYPACT Optogenetics httpwwwbbecaltecheducontentviviana-gradinaru httpglabcaltechedu Vladislav Verkhusha - near infrared fluorescent proteins httpwwweinsteinyuedulabsvlad-verkhushadefaultaspx httpwwweinsteinyueduresearchfacilitiesfluorescent WE Moerner ndash Stanford University httpwebstanfordedugroupmoerner Willard Wiggans ndash micro gallery httpwwwwillard-wigancomgallery Wolf Frommer ndash Molecular sensors httpbiosensordpbcarnegiescienceedu (fluorescent protein biosensors table) Xiaowei Zhuang = Harvard httpzhuangharvardedu Yuval Garini - Bar-Ilan University - Nano-Bio-Photonics laboratory httpgarinilabbiuacil Microscopy Magazines Imaging amp Microscopy httpwwwimaging-gitcommagazine Laser Focus World httpwwwlaserfocusworldcomindexhtml Microscopy amp Analysis httpwwwmicroscopy-analysiscom Microscopy Today httpwwwmicroscopy-todaycom Microscopy-UK httpwwwmicroscopy-ukorguk Photonics Spectra Biophotonics httpwwwphotonicscomPublicationsaspx The Microscope Journal httpmcriorgv72the-microscope-journal RMS Infocus Magazine httpwwwrmsorgukstudy-readinfocus-magazinehtml Nobel Prizes in Light Microscopy and Related Fields Chemistry 1925 colloids (light sheet) Zsigmondy (Siedentopf Zsigmondy 1902) httpswwwnobelprizeorgnobel_prizeschemistrylaureates1925
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012
TGP (Thermal Green Protein) 493 507 64000 066 42 126
Topaz 514 527 94500 06 57 169
UnaG 498 527 77300 051 39 117
VafLOV 450 495 12500 023 3 009
Venus2 (V2) 515 528 184000 057 105 312
Venus3 (V3) 515 528 276000 057 157 468
Venus4 (V4) 515 528 368000 057 210 624
Venus5 (V5) 515 528 460000 057 262 780
Venus6 (V6) 515 528 552000 057 315 936
Violeta (GFPmut3W66) 350 425 na na na na
vsfGFP-0 (dimer) 485 510 209000 076 159 473
vsfGFP-0 (monomer) 485 510 104500 070 73 218
WasCFP 492 505 51000 085 43 129
WiPhy (Wi-Phy) 700 722 118000 006 7 021
WiPhy2 (Wi-Phy2) 696 719 118000 009 10 031
YPet 517 530 104000 077 80 238
zFP506 Zoanthus sp 496 506 35600 063 22 067
zFP538 Zoanthus sp 528 538 20200 042 8 025
Table ignores photostability (photoinstability) molecular weight monomer vs dimer vs tetramer oligomerization
aggregation prone-ness pKa (pH sensitivity) and more Jellyfish (EGFP) coral Amphioxus etc fluorescent proteins
require one or two oxygen molecules to manufacture the chromophore bonds (a product is H2O2) LOV proteins require
Flavin cofactor Wi-Phy iRFPs UnaG require biliverdin This table also excludes photocontrollable fluorescent proteins
photoactivatable photoconvertible photoswitchable Cranfill et al (2016) published an experimental direct comparison of
several popular fluorescent proteins with literature comparisons NTnC GECI (genetically engineered Ca++ ion indicator)
is used as an example of a small state of the art fluorescent protein biosensor also shown is Ca++ ion insensitive double
mutant control (Barykina et al 2016) NTnC is an inverse signal (No Ca++ bright Ca++ saturated ~50 brightness) FP
biosensor ndash the paper shows simultaneous use with R-GECO (red fluorescence dark to bright) FP biosensor ndash these
could be used together to enhance ratiometric response Subcellular localization ex to cytoplasmic side of plasma
membrane could be used to further improve signal-to-noise ratio and provide compartment specific data The NTnC
Ca++ insensitive double mutant and mCherry (R-GECOrsquos parent Zhao et al 2011) could be simultaneously localized and
concentrated to the nucleolus Golgi body or other lsquoout of the wayrsquo compartment PubMed
(httpswwwncbinlmnihgovpubmed) Google ScienceDirect Highwire Press and other search engines and publishers
can be used to find full text publications of all fluorophores and fluorescent proteins listed Photo-changeable fluorescent
proteins are reviewed in Turkowyd et al (2016) and summarized in Lambert T Thorn (2016) SandercyaninBLA is a
lipocalin family member non-fluorescent apo-protein is 18 kDa fluorescent tetramer is 75 kDa quantum yield in 70
D2O is 166x that of in H2O (00267 vs 0016) (Ghosh et al (2016)
From the SelectedWorks of George McNamara
Fall October 3 2016
McNamara 20161006 revised draft tables for CPHG Light Microscopy and Image Analysispdf
tmp2O7op4pdf
Chemistry 2000 conductive polymers Heeger MacDiarmid Shirakawa (Brilliant Violets Ultraviolets Blues) httpwwwnobelprizeorgnobel_prizeschemistrylaureates2000 Chemistry 2008 green fluorescent protein GFP Shimomura Chalfie Tsien httpwwwnobelprizeorgnobel_prizeschemistrylaureates2008 Chemistry 2014 super-resolved fluorescence microscopy Betzig Hell Moerner httpswwwnobelprizeorgnobel_prizeschemistrylaureates2014 Physics 1953 Phase Contrast Zernike httpswwwnobelprizeorgnobel_prizesphysicslaureates1953 Physics 2009 invention of an imaging semiconductor circuit ndash the CCD sensor Kao Boyle Smith httpwwwnobelprizeorgnobel_prizesphysicslaureates2009 Physics 2014 efficient blue light emitting diodes Akasaki Amano Nakamura httpwwwnobelprizeorgnobel_prizesphysicslaureates2014 Physiology or Medicine 2006 Fire and Mello RNA interference ndash gene silencing by double stranded RNA httpwwwnobelprizeorgnobel_prizesmedicinelaureates2006 Physiology or Medicine 2006 Capecchi Evans Smithies specific gene modifications in mice by the use of embryonic stem cells httpwwwnobelprizeorgnobel_prizesmedicinelaureates2007 Physiology or Medicine 2009 Blackburn Greider Szostak how chromosomes are protected by telomeres and telomerase httpwwwnobelprizeorgnobel_prizesmedicinelaureates2009 Physiology or Medicine 2012 Gurdon Yamamanka matured cells can be reprogrammed to become pluripotent httpwwwnobelprizeorgnobel_prizesmedicinelaureates2012