McGill Radiobiology tutorial session: Assays for measuring radiation survival following irradiation Stanley Liu, MD, PhD Depts. Of Medical Biophysics and Radiation Oncology, University of Toronto Sunnybrook Odette Cancer Centre, Sunnybrook Health Sciences Centre
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McGill Radiobiology tutorial session: Assays for … · Assays for measuring radiation survival following irradiation Stanley Liu, MD, PhD Depts. ... Hall E. Radiobiology for the
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McGill Radiobiology tutorial session:
Assays for measuring radiation survival
following irradiation
Stanley Liu, MD, PhD Depts. Of Medical Biophysics and Radiation Oncology, University of Toronto
Sunnybrook Odette Cancer Centre, Sunnybrook Health Sciences Centre
Overview
Assays for radiosensitization
• In vitro – Radiation clonogenic survival assays
• In vivo – Tumor growth delay
– TCD50
– Ex vivo clonogenic survival
Joiner M & van der Kogel A (eds). Basic Clinical Radiobiology. Edward Arnold 2009.
Cell death after IR
Mitosis
DNA
Damage
Response Apoptosis
Senescence Autophagy
Necrosis
Mitotic Catastrophe
Clonogenic
Survival
Adapted from Wouters B (2015). Molecular Basis of Cell Death. Clin and Exp Rad
Bio Course U of T; and Minafra & Bravatà. Trans Cancer Res. 3(1): 2014
Clonogenic Survival Assay
Day 1:
Seed cells
Day 2 :
radiation treatment
~ Day 10:
stain and count
Munshi et al. Methods in Molecular Medicine vol 110.
Plotting clonogenic assay
Example of clonogenic assay to assess differential survival
Joiner M & van der Kogel A (eds). Basic Clinical Radiobiology. Edward Arnold 2009.
In vivo assays
Hallahan and Weinberg. Cell 2000
In vivo assays
–Tumor growth delay
–TCD50
– Ex vivo clonogenic survival
Tumor growth delay
Inject tumor cells SC into flank of
nude mouse
Irradiate tumor
measure tumor volumes
Tumor will typically regress in
volume then regrow
Tumor growth delay
Hall E. Radiobiology for the Radiologist. Tumor regresses in volume then regrows after IR
Tumor growth delay
TCD50
Joiner M & van der Kogel A (eds). Basic Clinical Radiobiology. Edward Arnold 2009.