Bellwork- have slate on desk ! 1. Explain how you would build this LEGO penguin? 2. What “rules” do you have to follow when building something with LEGOs? (i.e. how do they work?) 3. What would happen if you didn’t have all of the right pieces? 4. Find the TATA box & transcribe the gene into mRNA (hint: a gene must code for more than one amino acids): GCT ATA GCG TAC ATG CTT TCA ACT CGA CGA TAT CGC ATG TAC GAA AGT TGA GCT 5. Translate the mRNA into an amino acid sequence using a codon wheel to find the secret word. 6. When you are finished work on the “Secret Messages” assignment
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Materials to Prep Set of Toobers & Tacks for partners (borrowed from Nadja w/ the Biotech project @ UA)
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Bellwork- have slate on desk!1. Explain how you would build this LEGO penguin?
2. What “rules” do you have to follow when building something with LEGOs? (i.e. how do they work?)
3. What would happen if you didn’t have all of the right pieces?
4. Find the TATA box & transcribe the gene into mRNA (hint: a gene must code for more than one amino acids):
GCT ATA GCG TAC ATG CTT TCA ACT CGA CGA TAT CGC ATG
TAC GAA AGT TGA GCT
5. Translate the mRNA into an amino acid sequence using a codon wheel to find the secret word.
6. When you are finished work on the “Secret Messages” assignment
Bellwork check
GCT ATA GCG TAC ATG CTT TCA ACT CGA
CGA TAT CGC ATG TAC GAA AGT TGA GCT
mRNA: CGC AUG UAC GAA AGU UGA GCUTranscription
Amino acids: --- start(M) Y E S stop --- Translation
Protein Folding (On back of notetaker)
After translation, amino acid chains must fold into the correct 3D shape before they can begin working in the cell.
Remember this?
Enzymes (a type of protein) must be the correct shape to carry out their function
Discuss with your neighbor: How might proteins
get folded into the correct shape?
Amino Acid Sequence!
Amino acids interact based on their
chemical structure
Example: Cysteines (C) always form a “bridge” which folds the protein
Remove the yellow hydrophobic amino acid closest to the top
Replace it with a white polar amino acid
Fix your amino acid folding (yellow must be on inside, white must be on outside)
Explain how your protein shape changed (or didn’t).
How would this affect your protein’s ability to do its job?
Where do mutations come from?
GCT ATA GCG TAC ATG CTT TCA ACT CGA
CGA TAT CGC ATG TAC GAA AGT TGA GCT
Where do mutations come from?
GCT ATA GCG TAC ATG CTT TCA ACT CGA
CGA TAT CGC ATG TAC GAA AGT TGA GCT
CGA TAT CGC ATG TAC GAA AGT TGA GCT
GCT ATA GCG TAC ATC CTT TCA ACT CGA
mRNA: CGC AUG UAC GAA AGU UGA GCU aa seq: --- start(M) Y E S stop ---
mRNA: CGC AUG UAG GAA AGU UGA GCU aa seq: --- start(M) STOP
No Protein!
Closure Finish filling in the table below
Name of process Where it occurs Type(s) of RNA involved
Summary of how it works
Transcription mRNA
CytoplasmThe info coded in mRNA is used to make an amino acid chain
Protein Folding none
Closure Finish filling in the table below
Name of process Where it occurs Type(s) of RNA involved
Summary of how it works
Transcription Nucleus mRNARNA Polymerase makes an mRNA copy of the DNA gene, starting right after the promoter (TATA). Introns spliced out of mRNA before it leaves the nucleus.
Translation Cytoplasm mRNA, rRNA, tRNA Ribosome reads the mRNA and matches it to the tRNA’s which bring correct amino acids. Forms a long amino acid chain.
Protein Folding Cytoplasm none
Amino acid chains fold into a specific pattern based on the way the side chains interact with one another
Another day….
Mutations critical reading BW
Mutations Worksheet
Complete the mutations worksheet to see the effect of different kinds of mutations on the final protein
When you are finished, pick up the new critter assignment & begin your rough draft (due the day of the test)
Closure
Draw a comic strip showing the major steps of protein synthesis (transcription, translation, protein folding)