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MASSIF 1, automated beamline with RoboDiff Beamline Energy Range Typical Beamsize (microns) ID30A /MASSIF-1 12.8keV 100 ID30A -3 12.9keV 10 ID30B 6 to 20keV 20 to 200
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MASSIF 1, automated beamline with RoboDiff · ROBODIFF AT MASSIF 1 ... Ricardo Hino Jose-Maria Clement Hervé Gonzalez Page 10 MASSIF 1, automated beamline with RoboDiff 8/2/2016,

Sep 25, 2018

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Page 1: MASSIF 1, automated beamline with RoboDiff · ROBODIFF AT MASSIF 1 ... Ricardo Hino Jose-Maria Clement Hervé Gonzalez Page 10 MASSIF 1, automated beamline with RoboDiff 8/2/2016,

MASSIF 1,

automated beamline with RoboDiff

Beamline Energy Range Typical Beamsize

(microns)

ID30A /MASSIF-1 12.8keV 100

ID30A -3 12.9keV 10

ID30B 6 to 20keV 20 to 200

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ROBODIFF AT MASSIF 1

Page 2 MASSIF 1, automated beamline with RoboDiff 8/2/2016, Didier NURIZZO

“Hands-off” End Station :

Diamond monochromator and Be

lenses

RoboDiff (Goniometer and

Sample Changer)

Pilatus3 2M

X-ray and rotation axis alignment

Automatic data Collection and

processing

ISpyB integration

Page 3: MASSIF 1, automated beamline with RoboDiff · ROBODIFF AT MASSIF 1 ... Ricardo Hino Jose-Maria Clement Hervé Gonzalez Page 10 MASSIF 1, automated beamline with RoboDiff 8/2/2016,

ROBODIFF AT MASSIF 1

Page 3 MASSIF 1, automated beamline with RoboDiff 8/2/2016, Didier NURIZZO

Page 4: MASSIF 1, automated beamline with RoboDiff · ROBODIFF AT MASSIF 1 ... Ricardo Hino Jose-Maria Clement Hervé Gonzalez Page 10 MASSIF 1, automated beamline with RoboDiff 8/2/2016,

ROBODIFF AT MASSIF 1

Page 4 MASSIF 1, automated beamline with RoboDiff 8/2/2016, Didier NURIZZO

Scheduling:

Shifts allocated to BAG

Book a slot on the MASSIF-1

Google calendar

A form + invitation

Send crystals for slot

Samples will be processed

within 3 working beam days

of slot

All sample info MUST be

entered in ISPyB

Page 5: MASSIF 1, automated beamline with RoboDiff · ROBODIFF AT MASSIF 1 ... Ricardo Hino Jose-Maria Clement Hervé Gonzalez Page 10 MASSIF 1, automated beamline with RoboDiff 8/2/2016,

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Page 5 MASSIF 1, automated beamline with RoboDiff 8/2/2016, Didier NURIZZO

User experiments linked to ISpyB

Dewar tracking

Safety coordination

Data collection and

processing

File transfer

Page 6: MASSIF 1, automated beamline with RoboDiff · ROBODIFF AT MASSIF 1 ... Ricardo Hino Jose-Maria Clement Hervé Gonzalez Page 10 MASSIF 1, automated beamline with RoboDiff 8/2/2016,

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Page 6 MASSIF 1, automated beamline with RoboDiff 8/2/2016, Didier NURIZZO

Mesh scan:

Aperture 50mm

37% transmission

700mm for 30 images in 8s

300mm for 8 lines

Analyzed with Dozor

Page 7: MASSIF 1, automated beamline with RoboDiff · ROBODIFF AT MASSIF 1 ... Ricardo Hino Jose-Maria Clement Hervé Gonzalez Page 10 MASSIF 1, automated beamline with RoboDiff 8/2/2016,

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Page 7 MASSIF 1, automated beamline with RoboDiff 8/2/2016, Didier NURIZZO

Rotation Axis Alignment :

Aperture 50mm

100% transmission

2 scans (0/180°) 400mm for 40

images in 4s

Horizontal Alignment :

Aperture 50mm

80% Attenuation

1 scan (90°) 500mm for 40

images in 5s

Page 8: MASSIF 1, automated beamline with RoboDiff · ROBODIFF AT MASSIF 1 ... Ricardo Hino Jose-Maria Clement Hervé Gonzalez Page 10 MASSIF 1, automated beamline with RoboDiff 8/2/2016,

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Page 8 MASSIF 1, automated beamline with RoboDiff 8/2/2016, Didier NURIZZO

EDNA characterisation:

Aperture 50mm

100% transmission

4 images 90° apart 1° / 0.267s

Analyzed with Dozor, Best /

Labelit/Mosflm including

crystal size

Page 9: MASSIF 1, automated beamline with RoboDiff · ROBODIFF AT MASSIF 1 ... Ricardo Hino Jose-Maria Clement Hervé Gonzalez Page 10 MASSIF 1, automated beamline with RoboDiff 8/2/2016,

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Page 9 MASSIF 1, automated beamline with RoboDiff 8/2/2016, Didier NURIZZO

Data processing:

EDNA / Fast / Parallel

processing

Molecular replacement / Rigid

body (Dimple)

Fragment Analysis (Smiles)

more than 15,000 samples collected since

September 2014

Page 10: MASSIF 1, automated beamline with RoboDiff · ROBODIFF AT MASSIF 1 ... Ricardo Hino Jose-Maria Clement Hervé Gonzalez Page 10 MASSIF 1, automated beamline with RoboDiff 8/2/2016,

ROBODIFF AT MASSIF 1

Structural Biology Group

David Von Stetten

Daniele de Sanctis

Stéphanie Malbet Monaco

Christoph Mueller-Dieckmann

Gordon Leonard

Nicolas Guichard

Mario Lentini

John Surr

Thierry Giraud

Hugo Caserotto

Julien Soudarin

Fabien Dobias

Beamline Control Unit Matias Guijarro

Antonia Beteva

Sebastien Petitdemange

EMBL

Matthew Bowler

Max Nanao

Andrew McCarthy

Florent Cipriani

Gergely Papp

Engineering Unit Pascal Theveneau

Werner Schmidt

Carole Clavel

Electronics Unit

Ricardo Hino

Jose-Maria Clement

Hervé Gonzalez

Page 10 MASSIF 1, automated beamline with RoboDiff 8/2/2016, Didier NURIZZO

Contact Deborah Davison ([email protected]

Matthew Bowler ([email protected])

Didier Nurizzo ([email protected])

Data Analysis Unit Olof Svesson

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Page 11 MASSIF 1, automated beamline with RoboDiff 8/2/2016, Didier NURIZZO