Marine pelagic protists revealed by metabarcoding · 2019-02-15 · Marine pelagic protists revealed by metabarcoding Karoline Saubrekka Supervisors ... From Caronet al. 2012 basedonBaldauf2008.

Marine pelagic protistsrevealed by metabarcoding

Karoline Saubrekka

SupervisorsProfessor Bente EdvardsenDoctor Anders K. Krabberød

• Eukaryotes

Protists

From Caron et al. 2012 based on Baldauf 2008.

Marine pelagicprotists 

0.2 – 200 µmPhytoplankton

Marine environmental monitoring

• Cornerstone of the marine foodweb• Predators, decomposers, parasites• Climate change• Eutrophication• Toxic species• Invasive species• Good taxonomer• Large (>15 µm)• Well preserved

MetabarcodingCollect water sample

• 2 year periode• 5 m

Collect water sample

Filtration

> 0.22 µm

Collect water sample

Filtration

DNA extraction

Amplify markerregion

Genetic marker: V4 region in the 18S ribosomal gene

Collect water sample

Filtration

DNA extraction

Amplify barcoderegion Sequencing

Sequence filtering

Clustering (OTU)

Taxonomic classification

DiversityCommunity stucture

Population dynamics

What groups of protists can be detected by metabarcoding in the inner Oslofjord?

How did the samples vary through thesampling period?

Revealed only by metabacoding: • Parasites• Naked celles 

Revealed only by light microscope: • Lack interspesific variation in the marker• Lack sequences in the referance database

Light microscope cell count data by NIVA (Norli & Eikrem, 2018)Metabarcoding data

Light microscope vs. Metabarcoding?Light microscope +  Metabarcoding

Thank you!