DS-13009A L2013-12903 Lumi-Phos HRP (PS-atto) is a proprietary acridan based chemiluminescent substrate for ELISA detection of horseradish peroxidase (HRP) conjugated molecules. Lumi-Phos HRP couples a broad dynamic range with rapid generation of sustained luminescence. Lumi-Phos HRP ELISA HRP Substrate Chemistry that brings meaning to light. CH 3 S S(CH 2 ) 3 O(CH 2 ) 3 SO 3 Na S(CH 2 ) 3 O(CH 2 ) 3 SO 3 Na N CH 2 Ph O HRP + Light O O N CH 2 Ph N CH 2 Ph CH 3 S Reaction Mechanism Y Excellent sensitivity – less than 5 pg/mL of target protein in plate based ELISA Y Rapid peak intensity – reduced assay time and increased throughput Y Sustained luminescence – timing is less critical Y Temperature insensitive – analytical results areminsensitive to temperatures from 22°C - 35°C reducing the need for precise temperature control 5 3 4 2 1 0 -1 -21 -20 -19 -18 -17 -16 -15 Log (moles HRP) Log (S-B) Figure 1. Lumi-Phos HRP couples a broad dynamic range with rapid generation of sustained luminescence and superior storage stability to provide unmatched convenience to the user. Excellent Sensitivity 1800 1500 1200 900 600 300 0 0 1 3 4 2 5 Time (min) Intensity (RLUs) Addition of Substrate to Enzyme Rapid Peak Intensity Figure 2. Lumi-Phos HRP reaches a sustained maximum light-output peak intensity within seconds of substrate addition. Signal intensities can be read at any time along the profile to produce linear calibration curves.
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DS-13009AL2013-12903
Lumi-Phos HRP (PS-atto) is a proprietary acridan basedchemiluminescent substrate for ELISA detection ofhorseradish peroxidase (HRP) conjugated molecules.
Lumi-Phos HRP couples a broad dynamic range with rapidgeneration of sustained luminescence.
Lumi-Phos HRPELISA HRP Substrate
Chemistry that brings meaning to light.
CH3S S(CH2)3O(CH2)3SO3NaS(CH2)3O(CH2)3SO3Na
N
CH2Ph
O
HRP + Light
O
O
N
CH2Ph
N
CH2Ph
CH3S
Reaction Mechanism
Y Excellent sensitivity – less than 5 pg/mL of target protein in plate based ELISA
Y Rapid peak intensity – reduced assay time and increased throughput
Y Sustained luminescence – timing is less critical
Y Temperature insensitive – analytical results areminsensitive to temperatures from 22°C - 35°Creducing the need for precise temperature control
5
3
4
2
1
0
-1-21 -20 -19 -18 -17 -16 -15
Log (moles HRP)
Log
(S-B
)
Figure 1. Lumi-Phos HRP couples a broad dynamic range with rapid generation of sustainedluminescence and superior storage stability to provide unmatched convenience to the user.
Excellent Sensitivity
1800
1500
1200
900
600
300
00 1 3 42 5
Time (min)
Inte
nsity
(RLU
s)
Addition of Substrate to Enzyme
Rapid Peak Intensity
Figure 2. Lumi-Phos HRP reaches a sustained maximum light-output peak intensity withinseconds of substrate addition. Signal intensities can be read at any time along the profile toproduce linear calibration curves.
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Product Specifications
Enzyme Horseradish Peroxidase (HRP)
Application ELISA
Sensitivity Low picogram to femtogram
Ordering Information
Description Catalog Number
Lumi-Phos HRP (100 mL) PSA-100
Lumi-Phos HRP (1 L) PSA-1000
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