LLTECH BREAST ATLAS 2012

1 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com

LLTech Light-‐CT Scanner Breast images 2012


•  Op8cal in-‐depth biopsies of gross 8ssue within minutes

•  1 µm 2D and 3D histopathological resolu8on

•  Easy explora8on, acquisi8on and rendering in DICOM format

•  Safe, non-‐invasive and non-‐destruc8ve process

Light-‐CT™ key benefits

Fast and non-‐invasive 3D in-‐depth structural and cellular imaging


Based on Full-‐Field Op8cal Coherence Tomography (FFOCT)

Combines microscope resolu8on with interferometry

High resolu8on in-‐depth C scans

Commercial device specs:

•  Excellent resolu8on: 1,5µm transverse, 1µm axial

•  70Hz max. tomographic frame rate – 0.8 mm x 0.8 mm

•  Penetra8on depth 200µm – 1mm depending on 8ssue scaZering

•  25 mm diameter sample size

•  Small footprint: Scanner and light source fit on 70 cm x 35 cm

Light Computed Tomography technology


Op8cal acquisi8on unit, moving ver8cally •  User friendly

acquisi8on so\ware

•  DICOM 2D and 3D Viewer

Movable tray with sample holder

X,Y moving stage

Joys8ck for easy control of X,Y, Z movements

White Light Source

Integrated wide field camera to take sample picture before

imaging

Light-‐CT™ Scanner for ex-‐vivo cellular imaging


BREAST IMAGING


Grainy aspect of normal fibrous 8ssue

Duct with calcifica8on

Lobule Adipocytes Vessel

© LLTech 2012 Courtesy of Hôpital Tenon, Paris, France

Healthy breast <ssue

7 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com © LLTech 2012 Figure 3 : Diagnosis decision tree for FF-‐OCT images on human breast tissue

Breast tissue reading tree


Breast Histology DCIS – Ductal Carcinoma in situ

Breast., Ductal Carinoma H&E vs LightCT on Fresh Tissue Hopital Tenon, France, August 2010

Lobules

Fat cells

1 cm


Breast Histology DCIS – Ductal Carcinoma in situ

Lobules

Duct with necrosiss


Breast Histology Invasive adenocarcinoma - Nodular tumor

Tumorous 8ssue: highly scaZering thin trabeculae aspect of fibers

Healthy 8ssue: grainy medium scaZering fibers


Breast Milk duct with thick wall

1 mm

3 mm

1

Milk Duct

2

Blood vessels

Traditional Histology

1 mm

Connective tissue

Fat cells


Breast Histology Identification of the milk duct structures

Milk Duct End

Fat Cells


Healthy breast <ssue

Lobule

Galactophorous duct cut longitudinally

Honeycomb configura8on of adipocytes



Collec8ng duct with secre8on in the lumen

Collec8ng duct in the retroareolar sec8on

Nipple -‐ Cross sec<on



Invasive adenocarcinoma with in Situ Component

Enlarged ducts filled with cells prolifera8on or necrosis

Highly scaZering thin trabeculae aspect of fibrous 8ssue

surrounding grey cellular zones



Elongated compressed ductules with slit-‐like lumen

Well delimited nodule with lobulated appearence

Fibroadenoma



Fibroadenoma

Enlarged ductules characteris8c of the lesion



The Light-CT scanner allows fast tissue Processing and Pathology Examination. It completes the tools available for pathologists

When Chemical Fixation is needed

(Mostly Diagnostic)

When Frozen Section is needed

(Mostly Intra-operative)

Biopsy 3D

Digital Image

2D Digital Image

Slide

15-12 mins Artefacts

Destructive

12-24 hours Expensive Destructive

5-8 minutes Non-Destructive

Tissue Scanner


The Light-CT could help reducing the number of re excision in breast cancer surgery whilst preserving the current habits of pathologists

Per surgery

LLTech’s analysis

Histology And/or

Immuno histochemistry

Further treatment

Post surgery

Surgical margin analysis Metastasis analysis (> 2mm) et

micro metastaisi : PN1mi > 0.2 – 2 mm <

Surgery OK OK

Per surgery Post Surgery

Cur

rent

tech

niqu

es

Up

to 4

0% o

f dou

ble

proc

edur

es

LLTe

ch L

ight

-CT

Sig

nific

ativ

e de

crea

se o

f do

uble

pro

cedu

res

Visual analysis, cryostat

Further treatment Surgery

OK

Cancerous cancerous

OK

Poor identification of metastasis

Up to 40% of double surgical procedures

Several days analysis Few minutes analysis

Surgeon Pathologist Pathologist

Decrease of double surgical procedures Cancerous Cancerous

No surgical margins analysis

Histology And/or

Immuno histochemistry

Node

Margin

Several days analysis Few minutes analysis

Surgeon Pathologist Pathologist


Contact Details

USA

LLTech Inc. 103 Carnegie Center Drive

Suite 300 Princeton, NJ 08540

USA

www.lltechimaging.com [email protected]

Phone :+ 1 609 955 3506

LLTech SAS 6, place de la Madeleine

75008 Paris - France

www.lltechimaging.com

[email protected]

Phone :+33 9 72 16 33 40

Europe

LLTECH BREAST ATLAS 2012

Health & Medicine