Liquid Handling: BioMek 3000; Protocol Plate Washer; SOP Fraction Collector; Description Prepared by: Bob Morrison STLCC- Instrumentation Specialist Original June08, Latest Revision Aug2012 STLCC_CPLS:Morrison 8/3/2012 Beckman Coulter BioMek 3K At BRDG Park R126B Fraction Collection FRAC -100 @ FV Plate Washer, Bio-Plex Pro II @BRDG used with BioPlex Array reader Slide 1 Select instrument picture to jump to that section of the SOP
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Liquid Handling: BioMek 3000; Protocol
Plate Washer; SOP Fraction Collector; Description
Prepared by: Bob Morrison
STLCC- Instrumentation Specialist
Original June08, Latest Revision Aug2012 STLCC_CPLS:Morrison 8/3/2012
Beckman Coulter BioMek 3K
At BRDG Park R126B
Fraction Collection
FRAC -100 @ FV
Plate Washer, Bio-Plex Pro II
@BRDG used with
BioPlex Array reader
Slide 1
Select instrument picture to jump to that section of the SOP
1. Always “Home all Axes” under the Instrument heading before work.
2. Drag and Drop Instrument Setup into the Methods View area which will start a new method, ex: Method1
7
Liquid Handling: Biomek; Method, Drag-Drop Labware to Deck Locations
STLCC_CPLS:Morrison 8/3/2012
For the Method you are creating, Drag needed labware to positions on the Deck. In this sample, a Reservoir is at P3 and a AP96_200uL is at P7. Note: To remove labware, drag that item to the Clear/trashbin on the screen
Note: ML1 = manual latch, AL1= autolatch
8
Liquid Handling: Biomek; Define Tools in their Rack Positions
STLCC_CPLS:Morrison 8/3/2012
Double Click on the Tool Rack to bring up available tools and drag them to their desired/current positions for this Method.
9
STLCC_CPLS:Morrison 8/3/2012
Double-click on Reservoir to enter properties and volumes.
2. Right Click on the tip to see popup menu and select measurement options
3. Note, hover on tip to adjust up/down measurement from bottom.
4. Use keyboard up/down to set precise values.
Dest1
STLCC_CPLS: Morrison 8/3/2012 13
Liquid Handling: Biomek; Adding a Move Labware Step
STLCC_CPLS:Morrison 8/3/2012
1. Drag “Move Labware” from the left menu to the Method section
2. Enter locations on deck for From and To positions.
14
Liquid Handling: Biomek; Finish Step, Estimated Time for Completion
STLCC_CPLS:Morrison 8/3/2012
1. Double-click on Finish in Methods screen and examine Estimated time to complete in status bar at bottom.
2. If there are Errors in the method they will be displayed here.
15
Liquid Handling: Biomek; Saving this Method
1. Use File-Save-as, then enter a name in the pop-up menu for this Method for future use.
STLCC_CPLS: Morrison 8/3/2012 16
Liquid Handling: Biomek; Simulation Mode Setup
1. Under the Instrument Tab at top, select “Hardware Setup”
2. In the popup menu, select “Simulate” for the Port.
3. Select Accept. 4. Be sure to reset this to COM1
after simulation to reconnect to the actual device
STLCC_CPLS: Morrison 8/3/2012 17
Liquid Handling: Biomek; Simulation Mode, Set View Parameters
1. Highlight “Simulate” in the left panel, then adjust these items;
2. Warp Factor = slower or faster than real time in simulation
3. Perspective = Nicest 4. Labware Texture > = finer detail 5. Max Poly size < = better detail 6. Blending , check 7. Show Camera navigation, check 8. Hardware accel, check 9. Hide front bridge, check 10. Then ACCEPT at top
STLCC_CPLS: Morrison 8/3/2012 18
Liquid Handling: Biomek; Simulation, Camera Control Buttons
1. RESET View, restores to start view 2. Bold Up arrow, zoom in 3. Bold Down arrow, zoom out 4. Up/Down/Left/Right movement 5. STORE camera/zoom positions for
future using RESTORE buttons above 6. RESET View if you get lost
STLCC_CPLS: Morrison 8/3/2012 19
Liquid Handling: Biomek; Simulation Mode, Restore Button #3 Example, Zoomed in view of tips
1. Zoomed view was STORED in position #3 2. Zoomed view is activated by selecting
RESTORE #3 button 3. Use RESET to go to original view at
startup
STLCC_CPLS: Morrison 8/3/2012 20
Liquid Handling: Biomek; Simulation, Lighting Control
1. During a simulation run, Hit the tilde (~) key on the keyboard to set or modify Global (ambient) light or local intensity of lighting effects during simulation
2. The menu shown here is enabled where you may adjust lighting conditions and then “APPLY” them to the simulation.
3. “WARP Factor” controls the speed of simulation.
4. “NAVIGATION” adds or removes the camera control buttons on the lower portion of the screen
STLCC_CPLS: Morrison 8/3/2012 21
Liquid Handling: Biomek; Running Simulation
1. Select the Green Arrow in the top menu bar to begin simulation mode.
2. Select the Red box to stop the simulation.
STLCC_CPLS: Morrison 8/3/2012 22
Liquid Handling: Biomek; Running the Method
1. Under the Instrument tab select Hardware Setup
2. After initialization, a popup menu will show tools available and the Method to be run
3. Ensure that COM1 is selected to connect to the device vs. simulators or other devices
4. Select Accept to start the run using the device (not simulation).
• Fraction Collector A fraction detector is a device that allows regular or specified samples to be taken from a column eluate and stored in a retrievable form. The storage vessels are usually small sample tubes or vials that are oriented in a rotating disk or in a moving belt, there movement usually being controlled by a microprocessor.
• On receiving a signal from the microprocessor, the next vial is placed under the column outlet and the eluate collected until receiving another signal from the computer. Once the properties of the chromatogram that describes the separation has been ascertained, then the collection program can be defined.
• The fractions can be collected on a basis of time either at regular intervals or a specific times to collect specific peaks. Alternatively the fractions can be collected by monitoring the detector output and when a peak starts to elute the fraction collector is activated and the peak collected in a specific vial. When the peak returns to base line the column eluate is then directed to waste until the next peak starts eluting.
• Fraction collectors are in common use with most liquid chromatographs. They are used to collect samples for further purification, subsequent exmination by spectroscopic techniques or for biological or organoleptic testing.