“Lighting the Way to Technology through Innovation” The Institute for Lasers, Photonics and Biophotonics University at Buffalo Emerging Opportunities In New Directions of Photonics: Nanophotonics and Biophotonics P.N.Prasad www.biophotonics.buffalo.edu www.biophotonics.buffalo.edu
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“Lighting the Way to Technology through Innovation”
“Lighting the Way to Technology through Innovation”. The Institute for Lasers, Photonics and Biophotonics University at Buffalo Emerging Opportunities In New Directions of Photonics: Nanophotonics and Biophotonics P.N.Prasad. www.biophotonics.buffalo.edu. NANOPHOTONICS. - PowerPoint PPT Presentation
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“Lighting the Way to Technology through Innovation”
The Institute for Lasers, Photonics and Biophotonics
Nonradiative Processes for Photonic Functions/Dynamics <10 nm
Optically Induced Photonics Functions/Dynamics sub wavelengths
Manifestations:
Size Dependent Optical Transitions
Novel Optical Resonances
Nano-control of Excitations Dynamics
Manipulation of Light Propagation
Nanoscopic Field Enhancement
NANOPHOTONICS
NANOPHOTONICS Paras N. Prasad
(John Wiley & Sons, April 2004)
SUMMARY OF CONTENTS
1. Introduction 2. Foundations for Nanophotonics 3. Near Field Interaction and Microscopy 4. Quantum Confined Materials 5. Plasmonics 6. Nanocontrol of Excitation Dynamics 7. Processing and Characterization of Nanomaterials 8. Nanostructured Molecular Architectures 9. Nanocomposites 10. Photonic Crystals 11. Nanolithography 12. Biomaterials for Nanophotonics 13. Nanophotonics for Biotechnology and Nanomedicine 14. The Market Place for Nanophotonics
Nanocomposites for Broad Band andEfficient Photovoltaic, Solar Cells
In collaboration with Smalyukh and Lavrentovich, ILC, Kent State University
Laser Tweezers for micro- and nano- manipulation and surface adhesion
Multiple trapping in water by one beamLetters composed in Liquid Crystal
Measurement of colloidal forces and defect line tension and in liquid crystal
Introduction to Biophotonics Paras N. Prasad
(John Wiley & Sons, 2003)
SUMMARY OF CONTENTS 1. Introduction2. Fundamentals of Light and Matter3. Basics of Biology4. Fundamentals of Light-Matter Interactions5. Principles of Lasers, Current Laser Technology, and Nonlinear Optics6. Photobiology7. Bioimaging: Principles and Techniques8. Bioimaging: Applications9. Biosensors10. Microarray Technology for Genomics and Proteomics11. Flow Cytometry12. Light-Activated Therapy: Photodynamic Therapy13. Tissue Engineering with Light14. Laser Tweezers and Laser Scissors15. Nanotechnology for Biophotonics: Bionanophotonics16. Biomaterials for Photonics
Drug tracking using TPLSM
Doxorubicin : Chemotherapy drug
LHRH Peptide : Targeting agent.
C625 : Two-photon Chromophore
TPLSM images of MCF-7 cells showing the intake of drug into cell over a time period of 50 minutes.
= 800nmAvg. Power < 15mW=~ 90 fsf =82 MHz
Confocal images of MCF 7 cells. The arrows indicate The location where the spectra were taken.
Nucleus
Membrane
Cytoplasm
LHTPR
AC
Spectra profiles of AC&LHTPR treated MCF-7 cell (inside the Nucleus, Cytoplasm and on the Membrane)
Localized spectroscopy was used to identify the localization of a chemotherapeutic drug and and one of its component, the carrier protein, inside human cancer cells.
The ratio between the two emission at ~490nm (From AN152:C625) and the Emission at ~590 (From LHRH:TPR) was studied in different cell lines as well as in different parts of a cell to understand the roll of LHRH in carrying the drug into the cells.
Excitation Source: Ti:Sapphire laser tuned to a center wavelength of 800nm.
FGFR1-eGFP
Pre -Bleach Post -Bleach Recovery
0 100 200 300 400
0
10
20
30
40Nucleus
NM
PM
Time (s)
Flu
ore
sce
nc
e R
ec
ov
ery
(%
)
35.55 to 39.9771.70 to 82.0350.82 to
57.10
95% Confidence
37.6376.5253.78t½
(s)
Plasma MembraneNuclear MembraneNucleus
FRAP : A technique to monitor protien Dynamics in Cells