Light Microscope Parts and Functions. A. Eye piece Contains the ocular lens Magnification 10x.

Light MicroscopeLight MicroscopeParts and FunctionsParts and Functions

A. Eye pieceA. Eye piece

Contains the Contains the ocularocular lens lens

Magnification 10xMagnification 10x

K. ArmK. Arm

Used to Used to supportsupport the microscope the microscope when carried.when carried.

B. Revolving Nose PieceB. Revolving Nose Piece

Holds the Holds the lowlow and and highhigh-power objective -power objective lenseslenses; can be rotated to change ; can be rotated to change

lenses so that a slide can be seen at lenses so that a slide can be seen at different magnificationsdifferent magnifications

C. Objective LensesC. Objective Lenses

Magnification ranges from 4x to 40xMagnification ranges from 4x to 40x

Scanning Objective LensScanning Objective LensMagnification 4xMagnification 4x

Total Magnification = Total Magnification =

Eyepiece x Objective LensEyepiece x Objective Lens

Total Magnification for scanning Total Magnification for scanning objective lens = objective lens =

Scanning Objective LensScanning Objective LensMagnification 4xMagnification 4x

Total Magnification = Total Magnification =

Eyepiece x Objective LensEyepiece x Objective Lens

Total Magnification for scanning Total Magnification for scanning objective lens = 40xobjective lens = 40x

Low Power Objective Low Power Objective LensLens

Magnification 10xMagnification 10x

Total Magnification = Total Magnification =

Low Power Objective Low Power Objective LensLens

Magnification 10xMagnification 10x

Total Magnification = 100xTotal Magnification = 100x

High Power Objective High Power Objective LensLens

Magnification 40xMagnification 40x

Total Magnification = Total Magnification =

High Power Objective High Power Objective LensLens

Magnification 40xMagnification 40x

Total Magnification = 400xTotal Magnification = 400x

E. StageE. Stage

Supports the Supports the slideslide being viewed being viewed

D. Stage ClipsD. Stage Clips

HoldsHolds slide in place on stage slide in place on stage

J. Coarse Adjustment J. Coarse Adjustment KnobKnob

Moves the stage up and down for Moves the stage up and down for focusing an imagefocusing an image

I. Fine Adjustment KnobI. Fine Adjustment Knob

Moves the stage slightly to Moves the stage slightly to increase the clarity of increase the clarity of the imagethe image

H. DiaphragmH. Diaphragm

Regulates the amount of Regulates the amount of light that light that enters the microscopeenters the microscope

G. BaseG. Base

Supports the Supports the microscopemicroscope

F. Light SourceF. Light Source

Projects light Projects light raysrays through the through the diaphragm, the diaphragm, the slide to be seenslide to be seen, ,

and the and the lenseslenses

THE ENDTHE END

2

How to use a microscopeHow to use a microscope Total Magnification: Total Magnification:

Eye piece x objective lens =Eye piece x objective lens =

Use the SCANNING objective lens first!!!Use the SCANNING objective lens first!!!

NEVER use the coarse adjustment knob NEVER use the coarse adjustment knob when you’re on HIGH power!!!!!!!!!!!!!!!!!!!!! when you’re on HIGH power!!!!!!!!!!!!!!!!!!!!!