LEPTOSPIROSES Shakera Sadiq Gill
LEPTOSPIROSES Shakera Sadiq Gill
Definition Weils disease Acute systemic infections of humans and
animals caused by various serovars of Leptospira interogans
Etiology Leptospires are
Motile Gram negative Spiral shaped Terminal hooks
Fletcher medium is used for culture Culturing at 28-30o C
The organisms are detected by dark field microscopy
Important human leptospiral serovars
L interrogans serovars Reservoirs Icterohaemmorrhagiae Rat
Gripotyphosa Mouse
Australis Mouse Pomona Cattle
Canicola Dog
Hardio cattle
Occurrence Worldwide distribution Prevalent in the tropics Carried by >100 animal species Wild animals can also be infected Excrete large number of leptospires in
their urine
Population at risk In human population peak incidence is
during summer and fall Agricultural workers Veterinarians Breeders Abattoir workers Butchers
People handling meat Cooks Dog owners Hunters Animal trappers Sewage workers
Transmission Skin lesions from professional exposure Swimming or walking with bare feet Bites of rats, mice, hamsters Animal urine voided at the time of bite is
most likely source of infection Lesions in the nose, mouth and mucosa
are also the portal of entry
Divers Swimmers Canal workers Leptospiroses also observed after
flooding Adventure tourists
Clinical manifestations Leptospires are able to invade any organ They damage epithelial cells by means of
cytotoxic glycoprotein and induce bleeding and ischemic lesions
90% of the infections are self limited nonspecific febrile illnesses
Incubation period is 5-14 days (range 2-26 days)
Leptospires follow a diphasic course Bacteremic phase Immune phase
Bacteremic phase Phase of 4-7 days Sudden bout of fever up to 40oC Chills Malaise Headache Meningeal irritation Non productive cough
Hepatospleenomegaly Mild jaundice Abdominal pain Loss of appetite Nausea Vomiting
Detection of leptospires in bacteremic phsase
In blood In CSF Kidneys Urine More than 50% of the cases the disease
goes into second immune phase
Immune phase 1-30 days period Meningitis Iridocyclitis Jaundice Renal failure Anemia
Leptospires can also cause miscarriage Mortality rate ranges from 5-40 %which is
due to hepatic or renal failure
Disease in animals Acute febrile illness Jaundice Anemia Haematuria Abortion Mostly asymptomatic
Diagnosis History including occupational and
recreational activities During first week organism is detected
from CSF or urine by culturing on fletcher medium
Guinea pigs and hamsters are used to propagate organism
Microagglutination-lysis is the test of choice for detecting antibodies against leptospires
ELISA Diagnosis in animals
Agglutination lysis test
Differential diagnosis In first week
Influenza Salmonellosis Brucellosis Malaria dengue
In second week Hepatitis Meningitis Glomerulonephritis
Therapy In severe cases
Pencillin G Ampicilin
In less severe cases Amoxicilin should be administered orally for 7
days
Animals should be treated with tetracycline and streptomycin
Prophylaxis Rat and mouse control Protective clothing Water tight boots ,gloves and goggles
should be used for high risk population Walking bare footed and swimming in
stagnant should be discouraged Chemoprophylaxis with doxycyclin is >95
efficacious
End