1 Lecture 6: DNA Binding Interactions 6.1 DNA‐DNA Interactions – Antisense (plus modifications) – Thermodynamics of DNA Duplex Formation – Nucleobase Binding Sites 6.2 Protein‐DNA Interactions – Amino acid‐nucleic acid interactions – Structures: Zn‐finger, helix‐turn‐helix, helix‐loop‐helix, leucine zipper 6.3 Small molecule‐DNA Interactions and modifications – Sequence‐Specific Binding by Small molecules (Polyamide code‐ reading) – DNA intercalation – Overview of Nucleic Acid Modifications – DNA oxidation and photodamage ANTISENSE OLIGOS Crooke ST, Annu. Rev. Med. 55, 61-95 (2004) (1)single‐strand DNA oligo passes through cell membrane and enters cytoplasm. (2)oligo enters nucleus. (3)oligonucleotide binds (hybridizes) to the target mRNA, forming a sense‐ antisense duplex. (4)formation of the duplex initiates the recruitment of the RNase H enzyme, an endogenous nuclease. (5)RNase H degrades the target mRNA, inhibiting target mRNA expression.
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Lecture 6: DNA Binding Interactionsn.ethz.ch/~nielssi/download/5. Semester/Biological Chemistry I Nucle… · Lecture 6: DNA Binding Interactions 6.1 DNA‐DNA Interactions – Antisense
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Lecture 6: DNA Binding Interactions6.1 DNA‐DNA Interactions