The Microcirculation & Lymphatic System
The Microcirculation & Lymphatic System
Blood Vessel Structure & Function
The major types of blood vessels are
Arteries
The large distributing vessels that bring blood to the body
Arterioles bring blood to the capillaries
Capillaries
The tiny vessels that distribute blood to the cells
Venules drain blood from the capillaries
Veins
The large collecting vessels that bring blood back to the heart
Capillary Beds
A capillary bed is a network of the body’s smallest vessels that run throughout almost all tissues, especially the loose connective tissue
This flow is also called a microcirculation
Structure of the Microcirculation
Capillary System
• Metarterioles
• Precapillary sphincter
• True capillaries
The microcirculation
This is transport of nutrients to the tissues and removal of cell excreta.
The small arterioles control blood flow to each tissue area
local conditions in the tissues in turn control the diameters of the arterioles.
Each tissue controls its own blood flow in relation to its individual needs
The walls of the capillaries are thin, constructed of single-layer, highly permeable endothelial cells
Therefore, water, cell nutrients, and cell excreta can all interchange quickly and easily between the tissues and the circulating blood
Structure of the Microcirculation and Capillary System
Each artery entering an organ branches six to eight times before the arteries become small enough to be called arterioles
Then the arterioles themselves branch two to five times, reaching diameters of 5 to 9 micrometers at their ends where they supply blood to the capillaries.
Metarteriole and precapillary sphincter
The metarterioles do not have a continuous muscular coat, but smooth muscle fibers encircle the vessel at intermittent points
At the point where each true capillary originates from a metarteriole, a smooth muscle fiber usually encircles the capillary
This is called theprecapillary sphincter
This precapillary sphincter. can open and close the entrance to the capillary
Capillary Beds
When the precapillary sphincters are relaxed, blood flows through the true capillaries and takes part in exchanges with tissue cells
Capillary Beds
When the precapillary sphincters are contracted, blood flows through the shunts and bypasses the tissue cells
Arterioles
The diameter of each arteriole is regulated in two ways:
Local factors in the tissues signal the smooth musculature to contract or relax, thus regulating the amount of blood sent downstream to each capillary bed
Sympathetic nervous system adjusts the diameter of arterioles throughout the body to regulate systemic blood pressure
Structure of the Capillary Wall
The wall is composed of endothelial cells and is surrounded by basement membrane
The total thickness of the capillary wall is only about 0.5 micrometer
The internal diameter of the capillary barely large enough for red blood cells and other blood cells to squeeze through
Two passageways connecting the interior of the capillary with the exterior
1. intercellular cleft
2. plasmalemmal vesicles
The cleft normally with a width of about 10 nanometers, slightly smaller than the diameter of an albumin protein molecule
The rate of thermal motion of water molecules, ions and small solutes is so rapid that all of these diffuse with ease between the interior and exterior of the capillaries through these the intercellular clefts
Pores" in the Capillary Membrane
Flow of Blood in the Capillaries-Vasomotion
Blood usually flows intermittently, turning on and off every few seconds or minutes
The cause of this intermittency is the phenomenon called vasomotion, which means intermittent contraction of the metarterioles and precapillary
Exchange of water and substances between the blood and interstitial fluid
The most important means by which substances are transferred between the plasma and the interstitial fluid is diffusion
Diffusion results from thermal motion of the water molecules and dissolved substances in the fluid
Lipid-Soluble Substances Can Diffuse Directly Through the Cell Membranes of the Capillary Endothelium
If a substance is lipid soluble, it can diffuse directly through the cell membranes of the capillary without having to go through the pores.
Such substances include oxygen and carbon dioxide
Non-Lipid-Soluble Substances Diffuse Only Through Intercellular "Pores" in the Capillary Membrane
Oxygen, CO2, small solutes, nutrients move across capillaries primarily through diffusion
Diffusion through Capillary Membrane
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Molecular Weight (Daltons)
Per
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Water
Albumin
The relative permeability of skeletal muscle capillary pores to different sized molecules substances through capillary membrane decreases roughly with the molecular size
NaCl
Urea
Glucose
Sucrose
Inulin
The capillaries in different tissues have extreme differences in their permeabilities
The membrane of the liver capillary sinusoids is so permeable that even plasma proteins pass freely through these walls, almost as easily as water and other substances
The membrane of the renal glomerular is permeable to water and electrolytes, but this is not true for the plasma proteins
Capillaries are grouped according to their “leakiness”
Most common intestine, kidneyLiver sinusoids
10-15 nm holes 50-80 nm holes 100-1000 nm holes
The Interstitium and Interstitial Fluid
The spaces between cells are called the interstitium
The fluid in these spaces is the interstitial fluid
It contains two major types of solid structures:
(1) collagen fiber bundles
(2) proteoglycan filaments
The collagen fiber bundles are extremely strong and therefore provide most of the tensional strength of the tissues
The proteoglycan filaments, however, are extremely thin coiled molecules
Fluid filtration across capillaries is determined by hydrostatic and colloid osmotic pressure
The hydrostatic pressure in the capillaries tends to force fluid through the capillary pores into the interstitial spaces
Osmotic pressure caused by the plasma proteins (called colloid osmotic pressure) tends to cause fluid movement by osmosis from the interstitial spaces into the blood
Also important is the lymphatic system, which returns to the circulation the small amounts of excess protein and fluid that leak from the blood into the interstitial spaces
Four Primary Hydrostatic and Colloid Osmotic Forces Determine Fluid Movement Through the Capillary Membrane
Starling forces are:
The capillary pressure (Pc), which tends to force fluid outward through the capillary membrane
The interstitial fluid pressure (Pif), which tends to force fluid inward through the capillary membrane when Pif is positive but outward when Pif is negative.
The capillary plasma colloid osmotic pressure (Πp), which tends to cause osmosis of fluid inward through the capillary membrane.
The interstitial fluid colloid osmotic pressure (Πif), which tends to cause osmosis of fluid outward through the capillary membrane
If the sum of these forces, the net filtration pressure, is positive, there will be a net fluid filtration across the capillaries
If the sum of the Starling forces is negative, there will be a net fluid absorptionfrom the interstitial spaces into the capillaries. The net filtration pressure (NFP) is calculated as:
NFP = Pc – Pif – Πp + Πif
The rate of fluid filtration in a tissue is also determined by the capillary filtration coefficient (Kf) which is a measure of the capacity of the capillary membranes to filter water for a given NFP and is usually expressed as ml/min per mm Hg net filtration pressure
The rate of capillary fluid filtration is therefore determined as:Filtration = Kf X NFP