Leafy Vegetables, WP3, 14 th February Leafy Vegetables, WP3, 14 th February Leafy Vegetables Leafy Vegetables WP 3: Evaluation WP 3: Evaluation Leader: Valérie Cadot, France GEVES : Variety and seed, study and control group Groupe d’Etude et de contrôle des Variétés Et des Semences
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Leafy Vegetables, WP3, 14 th February Leafy Vegetables WP 3: Evaluation Leader: Valérie Cadot, France GEVES : Variety and seed, study and control group.
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– Material selected for the evaluation activities of the 4 cropsMaterial selected for the evaluation activities of the 4 crops– Seeds sent to the partners Seeds sent to the partners
2008, June: 2008, June: 1st year of evaluation1st year of evaluation 2009, December: 2009, December: 2nd year of evaluation2nd year of evaluation
2010, December: 2010, December: 3rd year of evaluation3rd year of evaluationData entered in a fixed format to be sent to the database Data entered in a fixed format to be sent to the database managersmanagers– Lettuce : Lettuce : 107/ 115 (contract)107/ 115 (contract)– Spinach: Spinach: 320320– Chicory: Chicory: 390 / 265 (contract)390 / 265 (contract)– Minor crops:Minor crops: 4949
C.C. Preparation of evaluationsPreparation of evaluations1.1. What is done by who?What is done by who?
2.2. How to select the material for evaluation How to select the material for evaluation (core collection)?(core collection)?
3.3. What about seeds? What about seeds? who sends to who?who sends to who? which quantity?which quantity?
4.4. What about methods? What about methods? if absent, which process? if absent, which process? harmonization when several methods?harmonization when several methods?
5.5. Which type of data will be included in the Which type of data will be included in the data base?data base?
a) Which criteria to select accessions?a) Which criteria to select accessions?
– based on maximizing the genetic diversity with with a limited set of accessions a limited set of accessions (core collection: often 5 to (core collection: often 5 to 20% of the whole collection)20% of the whole collection)
– based based on the purpose of evaluationson the purpose of evaluations ex : ex : Bremia lactucaeBremia lactucae: old cultivars without Dm genes : old cultivars without Dm genes (durable resistance).(durable resistance).
– to use to use the same accessions (or subgroups) for the same accessions (or subgroups) for the different evaluationsthe different evaluations while taking into while taking into account the purpose of each one.account the purpose of each one.
Pragmatic considerations: - the group size: ex: L strategy (logarithmic)
- availability of seeds- quality of documentation: passport data computerized curator’s knowledge about the phenotype - use of needs - reputation: standard,…
A mixture of different strategies based on diversity, use and numbers
5 steps 5 steps for the selection of a core collectionfor the selection of a core collection(see « Core collections of plant genetic resources », IPGRI)
1.1. Identify the material that will be Identify the material that will be presentedpresented
2.2. Decide on the size of the core collectionDecide on the size of the core collection
3.3. Divide the set of material used into Divide the set of material used into distinct groupsdistinct groups
4.4. Decide on the number of entries per Decide on the number of entries per groupgroup
5.5. Choose the entries from each group that Choose the entries from each group that will be included in the core.will be included in the core.
– If method absent, do we use the If method absent, do we use the method of another partner? method of another partner? Ex : Lettuce: Taste (organoleptic) and qualityEx : Lettuce: Taste (organoleptic) and qualityP7: No method P7: No method use P9 method?use P9 method?
– Harmonization when several methods?Harmonization when several methods?Ex: ChicoryEx: Chicory::