LABORATORY SAFETY LABORATORY SAFETY
8/4/2019 Lab Safety Lesson 2
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LABORATORY SAFETYLABORATORY SAFETY
8/4/2019 Lab Safety Lesson 2
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Safe laboratory procedures and
training are needed for everyone!
Safe laboratory procedures and
training are needed for everyone!
8/4/2019 Lab Safety Lesson 2
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WHY IS LABORATORY SAFETY
IMPORTANT?
WHY IS LABORATORY SAFETY
IMPORTANT?
TO PREVENT:
± adverse health effects from exposure to
chemicals ± exposure to organisms, diseases, etc. in
laboratories
± laboratory equipment hazards - if not
maintained properly
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LAB SAFETY COURSE OBJECTIVESLAB SAFETY COURSE OBJECTIVES
To show importance of written laboratory
program
To show importance of safety equipmentand personal protective equipment
To show importance of care of equipment
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Lab safety must be taught to :Lab safety must be taught to :
± all employees, including service groups
± undergraduate students
± graduate students
± professors
± visitors
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REVIEW LABORATORY
SAFETYWH
EN:
REVIEW LABORATORY
SAFETYWH
EN: new employees
new procedures
a change in procedures
new equipment
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LAB SAFETY CONT..LAB SAFETY CONT..
LABOR ATORY PROCEDURES
MUST BE SITE SPECIFIC !
based on your lab needs,
conditions, and equipment
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Laboratory Policies and Procedures
Must be:
written and available
Must be:
written and available
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AREAS TO COVER AREAS TO COVER
General procedures or rules
Glassware
Material handling and care Equipment
Safety equipment
Electrical safety Disposal procedures
Emergency response plans
Inspections
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General Procedures or Rules:General Procedures or Rules:
usually common to all areas
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General Procedures or Rules:General Procedures or Rules:
± no food or drink allowed in the laborator
± no mouth pipetting
± individualsnot
allowed to work in thelaboratory alone
± wear required personal protective
equipment
± no smoking in laboratory
± maintain good housekeeping habits
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GLASSWARE PROCEDURESGLASSWARE PROCEDURES
Storage
Proper use
Cleaning
Cleaning up broken glassware
Disposal of broken glassware
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Glassware Handling
Is this what your lab looks like?
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Glassware handling contGlassware handling cont
Or does it look like this?
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Laboratory MaterialsLaboratory Materials
Can include
± chemicals
± plants
± animals
± pathogens ± organisms
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Procedures for handling chemicalsProcedures for handling chemicals
± proper labeling, including wastes
± proper storage
storage cabinets
store compatible chemicals together rooms properly vented & correct temperature
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Procedures for handling chemicals cont.Procedures for handling chemicals cont.
Maintain a current inventory
Purchasing procedures
Proper handling
± use label or MSDS
± never test by taste or odor
± acids poured into water never vice versa
± take precautions and use proper equipment whenstirring or heating flammable liquids
± follow ³industry standards´ for labeling all
chemicals
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Handling animals and plantsHandling animals and plants
Procedures for caring for animals and plantsincluding feeding and watering
Procedures for cleaning cages
Procedures for cleaning and/or decontaminatingrooms or locations
Procedures for entering & leaving contaminatedareas
Procedures for handling the animals or plants
Procedures for animal bites or scratches
Procedures for disposal to prevent spread of disease
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Handling of diseases or organismsHandling of diseases or organisms
Allow only authorized individuals in infectious
disease laboratories
Do not allow individuals to work alone
Procedures for proper use of equipment and
maintenance
Use proper containers for transportation, incubation,
and storage
Labeling of laboratories and cultures
Proper disinfecting procedures
Hygiene procedures
Procedures for exposure or release of material
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Handling and Using Lab EquipmentHandling and Using Lab Equipment
± Proper installation
± Training on proper
use
± Manuals or written
procedures
available
± Inspections
± Maintenance
± DOCUMENT
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EQUIPMENT INCLUDES:EQUIPMENT INCLUDES:
Meters
Refrigerators
Autoclaves
Scales
Hoods
Drying oven
Compressed gas
cylinders Bunsen burners
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Lab Equipment
Which picture represents a proper hood?
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SAFETY EQUIPMENTSAFETY EQUIPMENT
± proper equipment must be available
± requires training for the location soindividuals know how and when to useequipment appropriately
± training on the correct
maintenance &storage is also
necessary
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SAFETY EQUIPMENTSAFETY EQUIPMENT
± FIRST AID AND
MEDIC AL TRT
± EMERGENCY
EQUIPMENT
± SHOWERS,
EYEW ASH
ST ATIONS
± MSDS¶S
± PPE
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First Aid and Medical TreatmentFirst Aid and Medical Treatment
First aid kit available and properly
stocked (nothing expired)
Trained first aid attendant or Medical facilities within 15 minutes
Emergency numbers posted
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Emergency EquipmentEmergency Equipment
Fire blankets
Fire extinguishers
Emergency notification system
Unrestricted means of communication
Any other emergency equipment
needed for your specific laboratoryneeds
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Emergency Showers &
Eyewash Stations
Emergency Showers &
Eyewash Stations
Immediate washing of the skin and eye
with generous amounts of water is themost effective first aid treatment for
chemical burns (unless chemical reacts
unfavorably to water-MSDS)
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Emergency Showers and Eyewash StationsEmergency Showers and Eyewash Stations
must be available
showers must be tested for proper
operation with results documented
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Alternatives to Installed Showers
and Eyewashes
Alternatives to Installed Showers
and Eyewashes Portable showers or eyewashes
Attachments that connect to existing
faucets
± must supply at least 15 minutes of
continuous water ± must stay on until turned off
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Material Safety Data Sheets, (MSDS)Material Safety Data Sheets, (MSDS)
± required for each
chemical
± requires employee
and student review
± must be accessable
for
employees/studentMSDS FILE
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MSDS Information Includes:MSDS Information Includes:
Nomenclature including chemical family
and formula
Hazardous ingredients
Physical data
Fire and explosion hazard
Health hazard Spill and leak procedures
Special protection information
Storage and handling precautions
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Personal Protective EquipmentPersonal Protective Equipment
AGENCY MUST:
± Provide PPE for all employees
at no cost to the employee ± train employees how to use PPE properly
± train employees on the limitation(s) of PPE
± train employees in proper care, storage,
and useful life, and disposal of PPE
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Appropriate PPE:Appropriate PPE:
± aprons,lab coats
± gloves-
latex,nitrile,neoprene
± goggles, face shields,
safety glasses
± respirators-full, partial,
dust mask
± noise protection
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ELECTRICAL SAFETYELECTRICAL SAFETY
± Protection of
employees and
equipment
± inspect panels and
plugs
± GFIs (specified by
code) ± surge protectors
± inspection &
reporting programs
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DISPOSAL PROCEDURESDISPOSAL PROCEDURES
± chemical wastes
± organisms, diseases, animals
± glassware
± spills ± sharps
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DISPOSAL PROCEDURES
CONT.
DISPOSAL PROCEDURES
CONT.
Trained persons designated to handle
disposal
Meet all required rules and regulations Proper collection containers
Waste collection contracts
Does not expose humans, animals,plants, etc upon disposal - may include
decontamination, sterilization,
incineration, autoclaving
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EMER GENCY RESPONSE PLANEMER GENCY RESPONSE PLAN
agencies should develop an
emergency response plan BEFORE an emergency
review with employees (students)
make sure they understand the plancompletely
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Emergency Response Plan should
include:
Emergency Response Plan should
include:
± recognizing emergencies
± lines of authority
± methods of communication
± safe sites and evacuation routes
± site security and control
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Emergency Response Plan should
include cont.:
Emergency Response Plan should
include cont.:
± decontamination procedures
± provisions for medical treatment
± emergency alerting and responseprocedures
± PPE and emergency equipment for
clean-up ± follow up
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INSPECTION OF LABORATORIESINSPECTION OF LABORATORIES
Develop inspection report appropriate
for laboratory
cover all areas related to laboratory ± personnel practices
± operational practices
± equipment ± emergency protection equipment
± materials inventory
± miscellaneous
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WEB ADDRESSWEB ADDRESS
http://www.doa.state.la.us/orm/lp.htm
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POST-TESTPOST-TEST
1. Laboratory equipment does not require
regular maintenance. T or F
2.
It is acceptable to eat and drink in thelaboratory. T or F
3. What is the most effective first aid treatment
for chemical burns?
4. Chemicals should be stored according tocompatibility. T or F
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POST-TESTPOST-TEST
5. Fume hoods should be used for chemical
storage. T or F
6.
What does MSDS stand for?7. What is some of the information an MSDS
can provide?
8. Electrical safety should not be ignored in
the laboratory. T or F9. Name PPE that might be used in the lab.
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POST-TESTPOST-TEST
10. Who should receive lab safety training?
11. The same laboratory procedures apply to
every lab. T or F12. Who is responsible for supplying PPE?
13. Inspections of laboratories are not
necessary. T or F
14. Chemical wastes can be disposed of in theregular trash. T or F
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POST-TESTPOST-TEST
15. There is no need to test emergency
showers. T or F
16.
Developing your emergency plan as anemergency is occurring is the best method
of development. T or F
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THANK YOU FOR ATTENDINGTHANK YOU FOR ATTENDING