Lab Safety Dr. G. Ciptadi,. Lab Genetika dan Pem. Ternak Dr. Marjuki : Nutrition Lab Dr. Lilik Eka R. Anim Tech. Prod. Lab Safety: Why is Important? Lab.

Lab SafetyDr. G. Ciptadi, .Lab Genetika dan Pem. Ternak

Dr. Marjuki : Nutrition Lab

Dr. Lilik Eka R. Anim Tech. Prod.

Lab Safety: Why is Important?

• Lab safety is a major aspect of every lab based science class.

• Lab safety rules and symbols are needed so that students do not injure themselves or their classmates.

Lab safety must be taught to :– all employees, including service groups

– Students

– professors

– Visitors/customer.

REVIEW LABORATORY SAFETY WHEN: REVIEW LABORATORY SAFETY WHEN: new employees new procedures a change in procedures new equipment

Good practice in Lab.

What are the general hazards in a laboratory?

• Fire• Breakage of glassware• Sharps• Spillages• Pressure equipment & gas

cylinders• Extremes of heat & cold• Chemical hazards• Biological hazards• Radiation

And many more!And many more!

When you take a job in a new laboratory, one of the first things you should do is ask your director to review with you the emergency response plans for the lab.

Make particular note of the locations of: Emergency telephone numbers. Eyewash fountains and emergency showers. Spill kits.Emergency exits and evacuation routes.

Emergency Response

Laboratory Equipment

• Never use any laboratory equipment unless you are trained & have been authorised to do so

• As well as injuring yourself you may cause very costly damage

LABORATORY PROCEDURES MUST BE SITE SPECIFIC

based on your lab needs, conditions,

and equipment

TYPES OF LABORATORIESTYPES OF LABORATORIES Pathology Chemistry : hazard Biology : live-dead cell Radiation Agrokompleks : Soils, animals

Personal PPE

You can do a lot to protect yourself while working with detergents and hazardous chemicals. Wear long pants, a long-sleeved shirt, closed-toe shoes, a laboratory coat, eye protection, and gloves.When handling acids wear a rubberized apron for added protection.

Biosafety ProgramPrinciples• General Lab Requirements

– Knowledgeable supervisor – Knowledgeable personnel

• Aware of potential hazards• Proficient in practices & techniques

– Lab specific biosafety manual– Biosafety Levels (BSLs)– Laboratory Practice and Technique– Safety Equipment (Primary Barriers)– Facility Design and Construction

(Secondary Barriers)– Biosafety cabinets (BSCs) - BSL 2/3– Personal protective clothing – Pipetting Devices – Safety centrifuge cups and rotors

2.2

• Well characterized, non-pathogenic organisms or agents

• Open bench- no containment• Use good laboratory practices, waste disposal,

and aseptic techniques

BioSafety Level 1

• Agents of moderate hazard to personnel or environment• Basic lab, but restricted access, containment during certain

processes (i.e. aerosols, large volumes, etc.)• Autoclave and Biological Safety Cabinet desired• Use good laboratory practices, waste disposal, and aseptic

techniques

BioSafety Level 2

• Agents of high hazard to personnel or environment

• Respiratory exotic or indigenous agents which are easily transmissible causing serious or lethal disease

• All work is contained, engineering controls and controlled environments we currently do not have the facilities to handle.

BioSafety Level 3

BioSafety Level 4 • FORGET ABOUT IT!!! • Hemorrhagic fever, deadly viruses, etc.

• Total containment, airtight labs, “submarine” doors, air pumps, water treatment, HEPA filtration, etc. Positive

pressure “moonsuits”

Practicing safe science with chemicals/Biological Material

Working with chemicals safely means doing all the things required for doing good science:

Know your protocol; execute procedures meticulously.Know your chemicals./Biological MaterialsUse smaller quantities of chemicals; consider saferreplacement protocols.Wear safety glasses, proper gloves, and a lab coat when handling hazardous chemicals; avoid splash hazards.Use a laboratory chemical hood when handling stock quantities of volatile hazardous chemicals.Be alert for hazardous situations; use a healthy dose of common sense; maintain concentration.

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Lab. Safety :Clothing

Wear:• Goggles/safety

glasses• Closed-toed shoes• Clothing made of

natural based fiber such as cotton

• Tie back long hair

Do NOT Wear:• Sandals• Jewelry• Loose or Baggy

clothing

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Lab Safety:Equipment

Safety Shower Safety GogglesEye Wash

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Lab Safety Equipment

Fire

Extinguisher Fire Blanket Fume Hood

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Lab Safety :SymbolsFound in your textbook

• Glassware• Heat• Eye and Face• Sharps

• Electrical• Animal• Chemical• Fire

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Other Chemical Hazards and Symbols

• Flammable

• Explosive

• Toxic/Poison

• Irritant

• Corrosive

• Environmental

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Chemical Hazard Symbols and Definitions

• Flammable – Any substance that will burn if exposed to an open flame.

• Explosive – A substance that may explode if exposed to heat or flame.

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Chemical Hazard Symbols and Definitions

• Toxic/Poison – A substance that can lead to death if inhaled, ingested, or absorbed by the skin.

• Corrosive – A substance that can destroy or burn living tissue and can eat away at other materials.

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Chemical Hazard Symbols and Definitions

• Irritant - A substance that causes inflammation upon contact with skin or mucous membranes.

• Environmental - Substances that are harmful to the environment. They must be disposed of properly, not washed down the drain.

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Accidents and Injuries

• Report ALL accidents and injuries to your teacher immediately!!!

• Be aware of safety hazards associated with each chemical you use.

• Eye accident – Wash the eye with copious amounts of water for at least 15 min.

• Fire Burns – Stop, Drop, and be covered with a fire blanket or soaked with water. Do not remove clothing stuck to victim. Get medical attention immediately.

DISPOSAL PROCEDURES– chemical wastes– organisms, diseases, animals– glassware– spills– sharps

Trained persons designated to handle disposal Meet all required rules and regulations Proper collection containers Waste collection contracts Does not expose humans, animals, plants, etc upon

disposal - may include decontamination, sterilization, incineration, autoclaving

Contoh : Carcasses and Body Parts

• Human tissues– Unfixed tissues are medical waste– Make waste unrecognizable!

• Animal tissues, carcasses– When generated in infectious disease or recombinant DNA research, these are

medical waste

• These items must be stored in biolabeled, leakproof containers for incineration.

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Reasons for proper waste disposal

• Hazardous chemicals down the drain can lead to pollution of ground water, lakes, rivers, etc.

• Plants and animals will die if they are exposed to hazardous chemical waste.

• Serious health problems will become present in people if hazardous waste finds its way into drinking water.

When in doubt – ASK!!!

• Do not carry out a new or unfamiliar procedure until you have been fully trained & understand the precautions necessary for safe working

• DO NOT DO NOT GUESS!!!!GUESS!!!!