Lab meeting 2014.02.24 이세라. Gene Scan –B2M Sample name 등록번호 Sample 날짜 양회식 7095224211/09 CRS 환자 신원홍 7095184911/16 CRS 환자 PCR.

Lab meeting

2014.02.24이세라

Gene Scan –B2MSample name 등록번호 Sample 날짜

양회식 70952242 11/09 CRS 환자

신원홍 70951849 11/16 CRS 환자

PCR

Sample 2.Sample 1.

electrophoresis

95℃ / 5min

Sequencing-PLK1

Childhood AML -RNA

cDNA합성

65 ℃ / 5min -> ice

25 ℃ / 10min -> 50 ℃ / 50min->85 ℃ / 5min -> ice

1μl of Rnase H 37 ℃ / 20min -> ice

cDNA->electrophoresis

cDNA PCR->electrophoresis

PLK1 -Primer

pyrosequencing

A-365B-2009-129D-4

D-22

2014.02.24(BDNF)

PCR전 PCR후2013.09.24(SLC6A4)

flowcytomety2014.02.21

G-MDSCM-MDSC

G-MDSCM-MDSC

2/20sample 조일도 71411924