Lab 2: Organelles, Proteins, and SDS-PAGE

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Lab 2: Organelles, Proteins, and SDS-PAGEDr. Sarah VanVickle-ChavezBIO 2960 LaboratorySpring 2013

+Overview

Today we will load a gel and compare your gel to the sample gel. Each person will load one lane on the gels in the center of each bench.

While gels are running, we will do the PyMOL computer lab as a group demo. You should have Lab Report 1a complete before we leave today.

You can use your books and notes, but there is a brief computer tutorial on the NSLC website to help you with Lab Report 1b.

http://www.nslc.wustl.edu/courses/Bio2960/labs.html

Next week, there are three reports due at the beginning of class 1a = proteins (PyMOL demo in class) 1b = cell organelles (use your book/notes for this one) 2 = SDS-PAGE

+1. Protein Electrophoresis

SDS-PAGE stands for sodium dodecyl sulfate polyacrylamide gel electrophoresis

+SDS vs Native Gel

How do you “see” your proteins?

+PyMOL Remember: Amino acids have different properties

based on the R-group

GAVLIMPFW = nonpolar

STNQYC = uncharged, polar

KRHDE = charged, polar

+Alpha helix

+Beta Sheet