Kuhlmann et al. Suppl. Figure SUVH2 (At2g33290) SUVH9 (At4g13460)
Kuhlmann et al. Suppl. Figure 1. Kuhlmann et al. Suppl. Figure 2 SRA-domainSET-domain Gabi-Kat_516A07 SALK_079574 At2g33290 SUVH2 SRA-domainSET-domain.
Mar 27, 2015
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Kuhlmann et al. Suppl. Figure 1
SUVH2 (At2g33290) SUVH9 (At4g13460)
Kuhlmann et al. Suppl. Figure 2
SRA-domain SET-domain
Gabi-Kat_516A07SALK_079574
At2g33290 SUVH2
SRA-domain SET-domainAt4g13460 SUVH9
SALK_048033
SALK_048093
LB-p1::SUL1-p35S> RB RB <p35S-SUL1::P1-LB
Gabi-Kat_516A07
TGAACGGCAA 1937bp 1946bp CTGCAACTAGpAC161
pAC161
Kuhlmann et al. Suppl. Figure 3
rel. mRNA SUVH2 / mRNA PFK
0,001
0,01
0,1
1
Col suvh2 suvh9 suvh2/suvh9
rel. mRNA SUVH9 / mRNA PFK
0,00001
0,0001
0,001
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0,1
1
Col suvh2 suvh9 suvh2/suvh9
rel mRNA act2 /mRNA PFK
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100
Col suvh2 suvh9 suvh2/suvh9
SUVH2 / PFK rel. RNA
SUVH9 / PFK rel. RNA
ACT2 / PFK rel. RNA
wt suvh2 suvh9 suvh2 suvh9
wt suvh2 suvh9 suvh2 suvh9
wt suvh2 suvh9 suvh2 suvh9
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C
mRNA of seedlings rel. to PFK mRNA (median with min and max deviation)
0,00001
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Col suvh2 suvh9 suvh2/suvh9
SUVH2
SUVH9
act2
mRNA of imbibed seeds rel. to act2 (median with min and max deviation)
0,00001
0,0001
0,001
0,01
0,1
1
Col suvh2 suvh9 suvh2/suvh9
D
E
wt suvh2 suvh9 suvh2 suvh9
wt suvh2 suvh9 suvh2 suvh9
Kuhlmann et al. Suppl. Figure 5
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Col-0 mycSUVH2
% m
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tion
Wt Pro35S-mycSUVH2 leaves leaves
A 100
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Col-0 Col-0 seedlings myc-SUVH2
% m
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Complete CG CNG CNN
% m
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yla
tion
Wt Wt Pro35S-mycSUVH2 leaves seedlings leaves
B100
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total C CG CHG CHH
Kuhlmann et al. Suppl. Figure 6
24mer -
wt
suvh
2 s
uvh9
wt
suvh
2 s
uvh9
AtSN1 (A)
tRNA
EtBr
Kuhlmann et al. Suppl. Figure 7
A B
C D
total C CG CHG CHH
PCR SUVH2
PCR SUVH9
PCR PFK
PCR ACT2
Kuhlmann et al. Suppl. Figure 8
Suppl. Table: Sequences of used primesPrimers used for genotyping:suvh2:SUVH2-gaby-F: ATATGCAGGTGTAGTTGTCACGAGSUVH2-gaby-R: ATGGCGAGCTTGCCGTTCACTTCT-DNA-insertion pAC161: pAC161 5´-out: ATATTGACCATCATACTCATTGCpAC161-F (NOS): GATGTCCGCAGCGTTATTATAAAATGPAC161-R (NOS): CGCATAATCTCAGACCAATCTGAsuvh9:SUVH9-SALK-F: ATGGGTTCTTCTCACATTCCTCTTGSUVH9-SALK-R: CAGTGATCCTCACTAGCTCCGACGT-DNA-insertion pROK2:pROK2 3´-out: GAAATATTTGCTAGCTGATAGTGpROK2-F (pNOS): GAGCGGAGAATTAAGGGAGpROK2-R (pNOS): GAGAACCTGCGTGCAATC
Primers used for qPCR:At4g04040 (Phospfofructokinase, Kapoor et al., 2005):F (B8F): gccacgaaaaccaaacagacR (B8R): ccggaatttcgatcaatcct At3g18780 (Actin-2 An et al., 1996):F: tgagagattcagatgcccagaaR: ttgattccagcagcttccatAt2g33290 (SUVH2):F: atatgcaggtgtagttgtcacgagR: atggcgagcttgccgttcacttcAt4g13460 (SUVH9):F: atgggttcttctcacattcctcttR: cagtgatcctcactagctccgacgAtSN1-A (AtSN1- Fragment A):F (F4) aaaataagtggtggttgtacaagcR (ATS15): accaacgtgctgttggcccagtggtaaatcAtSN1-B (AtSN1- Fragment B):F (A205): tgagagatttaccactgggccaacaR (A206): tgaggagctcaacacataaatggcaataAtSN1-C (AtSN1- Fragment C):F (A207): cctttccaagacaccatctcaacaacR (A208): tcctcaacaaaaataattccgaacgac
Primers used for converted target regions: AtSN1 (chromosome III, nucleotides 15,805,617–15,805,773) :AtSN1-bi-F: caatatacratccaaaaaacarttattaaaataatatcttaa (JP 1821, Johnson et al., 2008)AtSN1-bi-R: gttgtataagtttagttttaattttayggatyagtattaattt (JP 1822, Johnson et al., 2008)AtCopia4:AtCopia4-bi-F: ggttgtytgtgttttttatggttyagattttata (JP 3100, Johnson et al., 2008)AtCopia4-bi-R: ataactraaccacarattcaracccattttcattt (JP 3101, Johnson et al., 2008)
Primers used for quantitative amplification of genomic regions after ChIP:At4g04040 (Phospfofructokinase, beta-subunit, Mathieu et al., 2003):F: gccacgaaaaccaaacagacR: ccggaatttcgatcaatcct At3g18780 (Actin-2 An et al., 1996):F: tgagagattcagatgcccagaaR: ttgattccagcagcttccatTa3-LTR (Johnson et al., 2002):F (JP1617): tagggttcttagttgatcttgtattgagctcR (JP1618): tttgctctcaaactctcaattgaagtttAtSN1-A (AtSN1- Fragment A, Herr et al., 2005):F (F4) aaaataagtggtggttgtacaagcR (ATS15): accaacgtgctgttggcccagtggtaaatcAtSN1-B (AtSN1- Fragment B):F (A205): tgagagatttaccactgggccaacaR (A206): tgaggagctcaacacataaatggcaataAtSN1-C (AtSN1- Fragment C):F (A207): cctttccaagacaccatctcaacaacR (A208): tcctcaacaaaaataattccgaacgac
Kuhlmann et al. Suppl. Table
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