KOTAGIRI MELODY, LIMGENCO MARTHA, MENDOZA MIGUEL, NEVADO PAOLO, SANDOVAL PATRICIA, SOLIVEN MONIQUE Effects of Diesel Inhalation, Alcohol Consumption and.

KOTAGIRI MELODY, LIMGENCO MARTHA, MENDOZA MIGUEL, NEVADO PAOLO, SANDOVAL PATRICIA, SOLIVEN MONIQUE

Effects of Diesel Inhalation, Alcohol Consumption and Sugar

Intake on Learning and Coordination

Introduction1) To be able to determine

the correlation of diesel inhalation, alcohol consumption, and sugar intake with the time needed to learn the course of a maze

2) To be able to determine the effects of diesel inhalation, alcohol consumption, and sugar intake on coordination.

Methodology

a. Alcohol Consumption

b. Diesel Inhalation

c. Sugar Intake

5%

10%

gauze

8 mL diesel

chicken wire cage

glucose

50%

5%

improvised gavage

improvised gavage

Results

Table 1. Effect of Alcohol Intake on Learning and Coordination

* 0.2 mL volume of 5% and 10% alcohol were administered through an improvised tube

Mouse Observations Time to Finish Maze (mm:ss)

1 (5% alcohol)* Normal movement speed, but kept missing the right path

7:50

2 (10% alcohol)* Lacking coordination in movement, followed the same path over and over again

More than 15 minutes

Control Normal 2:20

Results

Table 2. Effect of Exposure to Diesel on Learning and Coordination

*amount of diesel 8 mL in Petri Plate covered in gauze

Mouse Observations Time to Finish Maze (mm:ss)

1 (15 mins.) Slow movements, didn’t eat food

03:01

2 (30 mins.) Slower movements, didn’t eat food

12:41

Control (no diesel inhalation)

Normal 02:10

Results

Table 3. Effect of Sugar Intake on Time to Finish the Maze

*A 15-minute cap was placed on the time, and Mouse 2 was not able to finish the maze.

Mouse Observations Time to Finish Maze (mm:ss)

1 (50% glucose) Faster movement, ate the food at the end of the maze

3:30

2 (5% glucose) Fast movement, hyperactive, climbed over the maze

15:00*

Control Normal 2:20

Figure 2. Effects of Three Variables on Time to Finish a Maze.

Discussion

a. Alcohol Consumption- Ethanol as a central nervous

system depressant- Nigrostriatal pathway- Memory, attention, sleep,

coordination, and judgment- Coordination: alcohol impairs

reaction time and mental acuity

- GABA and NMDA

Discussionb. Diesel Inhalation- Diesel vapour inhalation:

effect on the mobility - Hydrocarbons : decreases

the sensitivity of postsynaptic neurons ; hypoxia

- Respiratory depressionc. Sugar Intake- Increased blood sugar levels

in their blood- Influence memory, motor

function and sleep patterns

 Conclusion

- Study verified hypothesis about alcohol effects - Sugar intake results induced hyperactivity but does not

give a direct relation to coordination and learning patterns

- assumption: hydrocarbons are responsible for the sluggish movements of the mouse.

- long-term effects may also develop from regular and high - concentration exposure of diesel fuel ﴾e.g. lipoid pneumonia).

- A detailed study is therefore recommended to determine the specific compound(s) in diesel fuel and the pathway impaired by this compound(s) which cause the signs of toxicity.