Japan and Thailand using Japan and Thailand using gas chromatography with gas chromatography with liquid-liquid extraction liquid-liquid extraction and solid-phase and solid-phase microextraction microextraction Kenji Kuwayama Kenji Kuwayama et al. et al.
Comparison and classification of methamphetamine seized in Japan and Thailand using gas chromatography with liquid-liquid extraction and solid-phase microextraction. Kenji Kuwayama et al. Present by. Lt.Sirikanya Ruangsri. 51312326. RESUME. NAME :. Lt.Sirikanya Ruangsri. - PowerPoint PPT Presentation
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Comparison and Comparison and classification of classification of
methamphetamine seized methamphetamine seized in Japan and Thailand in Japan and Thailand
using using gas chromatography with gas chromatography with liquid-liquid extraction liquid-liquid extraction
and solid-phase and solid-phase microextractionmicroextraction
Kenji Kuwayama Kenji Kuwayama et al.et al.
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INTRODUCTION
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Methampheta
mine
Caffeine Amphetamine
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Methampheta
mine
Amphetamine
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eat inject smoke inhale
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HN
OH
O
HN
Methamphetamine
1-phenyl-2-propanone (P2P)
Ephedrine compound
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Presumptive Test Thin Layer Chromatography (TLC) Gas Chromatography Flame Ionization Detector (GC-FID) Gas Chromatography Mass Spectrometry (GC-MS) High Performance Liquid Chromatography (HPLC) Fourier Transform Infrared (FTIR) Spectroscopy
ท่ีมา : United Nations Office on Drugs and Crime
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Two GC method for MA impurity profiling
NRIPS ONCB
The national Research Intitute of Police Science
The Office of the Narcotics Control Board
JAPAN THAILAND
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LLE
JAPAN THAI
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Chromatograms obtained using the ONCB method
tablet
crystal
THAI
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Chromatograms obtained from an MA crystal
ONCB
NRIPS
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The aim of this study
•Improve the analytical method for profiling MA impurity
•Compare and classification MA crystals seized in different countries
•Information in criminal investigation ; traffic routes , the source of supply and relationships between seizures
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MethodMethod
Liquid-Liquid Extraction( LLE )
Solid-Phase Microextraction
(SPME )
GC-FID & GC-MS
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Technique LLE
sample
centrifuge
Shaking
solution
Organic layer
GC
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Technique SPME
SPME fiber
SPME fiber
GC
Head space
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FID
Technique GC-FID
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Technique GC-MS
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MATERIALS AND
METHODS
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Reagents and chemical1. MA.HCl crystals seized in Japan
(69) and Thailand (42)2. Std. d-MA.HCl3. l-ephedrine.HCl4. dl-Dimethylamphetamine.HCl5. cis-1,2-dimethyl-3-phenylaziridine6. n-Alkanes : Internal Standard7. Solvents 8. SPME holder and fiber coated with
Typical chromatograms of MA samples in each group using the
NRIPS
Group C
Group D
cis-1,2-dimethyl-3-phenylaziridine
Ephedrine (pseudoephedrine)
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3.Comparison and classification of sample in the high purity group by
SPMEWhen MA crystals were extracted with SPME and analyzed by GC (SPME ), the chromatograms had many impurities while the chromatograms in case of LLE had few impurities
Characteristic peaks for data processing seven peaks
Many impurity
MA
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3.Comparison and classification of sample in the high purity group by
SPME
15/19
NRIPS
SPME
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Chromatograms were distinguished clearly from SPME method , whereas in the case of LLE it was difficult to compare and classify samples in the high-purity group because there were so few impurities.
The SPME method enables us to compare and classify high-purity
MA
3.Comparison and classification of sample in the high purity group by
SPME
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CONCLUSION
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ComparedLLE
ONCB
NRIPSSuperior for detecting and separating MA impurities
Classified into four groups
14 peakscharacteristic
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LLE
SPMEEffective for comparing high-purity MA because it detected many characteristic peaks