JOURNAL OF BACTERIOLOGY · JOURNAL OF BACTERIOLOGY VOLUME 119 * NUMBER 3 * SEPTEMBER 1974 EDITORIAL BOARD L. LEONCAMPBELL,Editor-in-Chief (1975) University ofDelaware, Newark R. L.

JOURNAL OF BACTERIOLOGYVOLUME 119 * NUMBER 3 * SEPTEMBER 1974

EDITORIAL BOARD

L. LEON CAMPBELL, Editor-in-Chief (1975)University of Delaware, NewarkR. L. ANDERSON, Editor (1975)

Michigan State University, East LansingS. G. BRADLEY, Editor (1979)

Virginia Commonwealth University, Richmond

ROY CURTISS III, Editor (1979)University of Alabama, BirminghamCARL SCHNAITMAN, Editor (1978)University of Virginia, CharlottesvilleH. E. UMBARGER, Editor (1976)Purdue University, Lafayette, Ind.

James N. Adams (1976)Arthur I. Aronson (1976)Stephen D. Barbour (1976)W. Lane Barksdale (1975)M. E. Bayer (1975)Blaine Beaman (1976)Claire M. Berg (1976)Richard S. Berk (1974)Winfried Boos (1976)Herbert W. Bover (1975)Jean Brenchley (1977)B. Wesley Catlin (1974)D. Joseph Clark (1974Stanely N. Cohen (1976)Ronald S. Cole (1974)S. F. Conti (1975)Nicholas R. Cozzarelli (1975)Walter J. Debrogosz (1976)R. G. Eagon (1974)Alan D. Elbein (1976)Wolfgang, Epstein (1974)Stanley Falkow (1975)Gerald R. Fink (1975)W. R. Finnerty (1976)A. T. Ganesan (1975)

Howard Goldfine (1975)Harry Gooder (1975)Joseph S. Gots (1975)Harlyn 0. Halvorson (1975)George Hegeman (1974)Donald R. Helinski (1975)Charles E. Helmstetter (1975)Joseph T. Holden (1975)Stanley C. Holt (1974)H. Ronald Kaback (1975)Sam Kaplan (1974)David E. Kennell (1974)Robert L. Lester (1975)Hillel S. Levinson (1975)E. C. C. Lin (1975)K. Brooks Low (1976)Elizabeth McFall (1976)Michael H. Malamy (1975)Manley Mandel (1975)David Mardon (1974)Robert E. Marquis (1976)Neil H. Mendelson (1975)M. L. Morse (1975)Eugene W. Nester (1975)Richard P. Novick (1976)

L. Nicholas Ornston (1975)Leo Parks (1976)Martin Pato (1975)Paul V. Phibbs (1976)Allen T. Phillips (1976)Lewis I. Pizer (1975)Jack Preiss (1974)Charles C. Remsen III (1976)William S. Reznikoff (1976)Antonio H. Romano (1976)Harold L. Sadoff (1974)M. R. J. Salton (1975)David Schlessinger (1974)Irwin H. Segel (1976)Jane K. Setlow (1975)Helen Jean Shadomy (1975)Stanley K. Shapiro (1976)Simon Silver (1976)W. R. Sistrom (1975)Lucile Smith (1975)Donald J. Tipper (1975)Arthur Weissbach (1974)Clifford A. Woolfolk (1976)Stanley A. Zahler (1974)Howard Zalkin (1976)

ROBERT A. DAY, Managing Editor, 1913 I St., N. W., Washington, D.C. 20006

EX OFFICIOPhilipp Gerhardt, President, (1974-1975)

J. Mehsen Joseph, Secretary

The Journal of Bacteriology, a publication of the American Society forMicrobiology. 1913 St., N.W., Washington, D.C. 20006, is devoted to theadvancement and dissemination of fundamental knowledge concerningbacteria and other microorganisms. The Journal is published monthly, andthe twelve numbers are divided into four volumes per year. The subscrip-tion price is S85 (Postal Union Countries, $87: other foreign, S88) per year.Single copies are S8.00 (foreign, 8.25). Members of the American Societyfor Microbiology may receive the Journal as part of their dues. Correspon-dence relating to subscriptions, reprints, defective copies, availability of backissues, lost or late proofs, disposition of submitted manuscripts, and gen-eral editorial matters should be directed to the ASM Publications Office.1913 St., N.W., Washington, D.C. 20006 (area 202 833-9416). Correspon-dence from ASM members relating to membership dues, member subscrip-

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AUTHOR INDEXAnderson, Richard L., 698Atlas, R. M., 868

Baptist, James N., 961, 976Bartha, R., 868Bazzicalupo M., 821Beveridge, T. J., 1019Bloch, Philip L., 736Bissett Donald L., 698Brenchiey, Jean E., 848Bronner, Felix, 840Buchanan, Bob B., 1066Burdett, i. D. J., 1039

Carsiotis, M., 889, 893Chatterjee, A. N., 672Cicmanec, John F., 718Cohen, Stanley N., 1072Cords, B. R., 830Cota-Robles, E. H., 1006

Davey, John F., 923, 930Deutch Charles E., 861Diedrich, D. L., 1006Doolittle, W. Ford, 677

Ennever, J., 1061

Favre, Ren6e 1069Funayama, Shigehiro, 1000

Geisler, Jarmila, 661Gerrard, T. L., 1057Gibson, D. T., 879, 923, 930Gilpin, R. W., 672Golub, Ellis E., 840

Hall, Ruth M., 1063Hancock, Robert E. W., 726Hash, John H., 913Hashimoto, Seiji, 811Hooper, Alan B., 899Hopper, Anita K., 952Howell, Neil 1063Hranueli, K., 684

Iaccarino Maurizio, 1069Inouye, Masayori, 907

Joenje, H., 784Johnson, Mary K., 844Jones, Richard F. 889, 893Jorstad, Caroline Mi., 857Jousimies, Hannele, 753

Kemper, Jost, 937Kessin, Richard H., 776Khachatourians, George G.,795

Komano, Tohru, 811Konings, W. N., 784Kooistra, Jan, 705Krieg, Noel R., 691

Laughon, Barbara E., 691Lederberg, Esther M., 1072Levinthal, Mark, 1069Liau, Deng-Fong, 913Lichstein, Herman C., 718Linnane, Anthony W., 1063Lorkiewicz, Z., 771Lukins, H. B., 1063

McKay, L. L., 830Magee, P. T., 952Mahadevan, Venkatanarayana,930

Makela, P. Helena, 753, 760, 765Mandelstam, J 684Manley, MandeI, 961, 976Matsumoto, Kouji, 666Mayer, H., 760, 765Melly, M. Ann, 913Michaels, G. E., 992Miller, Sara E., 992Morris, David R., 857Murray, R. G. E., 1019,1039

Narrod, S., 672Neidhardt, Frederick C., 736Neter, E., 760Newell, Peter C., 776

O'Brien, R. W., 661

Parisi, B., 821Patel, T. R., 879Paul, Aniko V., 907Pauling, Crellin, 861Pemberton, J. M., 748Piggot, P. J., 684Pirnik, M. P., 868Prasad, Chandan, 805

Reeves, Peter, 726Rogers, P. J., 653Russa, R., 771

Saito, Hiuga, 666Sakai, Hiroshi, 811Sala, F., 821Shaw, Charles R., 976Shibata, Takehiko, 666Singer, Richard A., 677Skurray, Ronald A., 726Smith, David F., 736Spurlock, Ben O., 992Steimer-Veale, Kathelyn, 848Stewart, P. R., 653Streckfuss, J. L., 1061

Telford, J. N., 1057Terry Kathleen R., 899Tevetiia, M. J., 961, 976Tipper, Donald J., 795Tu, J. C., 986

Venema, Gerard, 705, 784Vogel, J. J., 1061

Wesseling, Anne C., 893Whang, H. Y., 760Williams, H. H., 1057Williams, Keith L., 776Wong, W., 672

Young, F. E., 672

Zancan, Glaci T., 1000

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FERMENTATION TECHNOLOGY TODAY

Proceedings of the IVth International Fermentation SymposiumEdited by Dr. Gyozo Terui

This volume contains all the papers presented at the focal topic sessions of theIV International Fermentation Symposium held in Kyoto during March 19-25,1972. Valuable, up-to-date information in the field of fermentation technology ispresented.

Copies of Fermentation Technology Today are now available. The volume was

published in May 1973.

Contents include:(1) Fermentation Dynamics; (2) Mass Transfer and Scale-up; (3) ContinuousCulture; (4) Instrumentation for Process Control; (5) Genetics Applied to

Process Improvement; (6) Amino Acids and Nucleic Acid-Related Products

by Fermentation; (7) Microbial Enzymes; (8) Water-Insoluble EnzymeDerivatives; (9) Physiologically Active Substances by Fermentation; (10)Biomass from Noncarbohydrate Organic Substances; (i 1) Utilization ofAutotrophic Microbes; (12) Microbiological Waste Treatment; (13) RecentProgress in Traditional Fermentation Industries; (14) Tissue Culture; (15)Novel Fermentation Products; (16) Production and Use of Enzymes Activein Hydrolyzing Cell Walls and Related Substances; (17) Process Design andProduct Recovery; (18) Taxonomy and Ecology of Yeasts; (19) SubcellularStructure and Function of Yeasts; (20) Sexuality, Gene Action, and Breedingof Yeasts; Opening and Closing Lectures.

Index of subjects and authors, 890 pages. Published by the Society of Fermenta-tion Technology, Japan. Distributed by the American Society for Microbiology.

Available at $18 per copy from:Publications OfficeAmerican Society for Microbiology1913 I Street, N.W.Washington, D.C. 20006

AUTHOR INDEX

VOLUME 119

Abe, Mihoko, 543Ackerman, Rollie S., 357Adams, James N., 646Adams, Margie M., 443Allen, Jane Smith, 443Allen, Robert G., 443Anderson, Richard L., 484, 698Aoyagi, Takaaki, 394Atlas, R. M., 868Avigad, Gad, 363

Baptist, James N., 961, 976Bartha, R., 868Bazzicalupo, M., 821Bendich, Arnold J., 547Benemann, John R., 258Beveridge, T. J., 1019Bhumiratana, Amaret, 484Bissett, Donald L., 698Bloch, Philip L., 736Bowers, Blair, 564Boye, Erik, 522Bracha, Moshe, 583Bragg, P. D., 129Bremer, Hans, 270Brenchley, Jean E., 448Brill, Winston J., 266Bronner, Felix, 840Brownstein, Barbara, 345Brustad, Tor, 522Buchanan, Bob B., 1066Burdett, I. D. J., 303, 1039

Cabib, Enrico, 564Campbell, Judith L., 494Carbonell, Luis M., 602Cardelli, James, 379Carsiotis, M., 889, 893Charnetzky, W. T., 162, 170,

176Chatterjee, A. N., 672Chilton, Mary-Dell, 547Chojnacki, Bonnie, 461Cicmanec, John F., 718Clark-Walker, D. D., 282Cohen, Stanley N., 1072Cooper, Terrance G., 461Cords, B. R., 830Costilow, Ralph N., 484Cota-Robles, E. H., 1006Cozzarelli, Nicholas R., 357Currid, Victoria R., 576

Dale, J. W., 351Datta, Naomi, 144Davey, John F., 923, 930

David, Hugo L., 527Dennis, Patrick P., 270Deutch, Charles E., 861Dickbuch, Sarah, 635Diedrich, D. L., 1006Doi, Osamu, 543Doi, Roy H., 514Doolittle, W. Ford, 677Doudney, C. O., 123Dugan, Patrick R., 76Dunn, Etta, 534Duntze, Wolfgang, 386

Eden, Francine C., 547Eidels, Leon, 138Eisenstark, A., 62Englard, Sasha, 363Ennever, J., 1061Ephrati-Elizur, E., 36Epstein, Wolfgang, 357

Falkow, Stanley, 144Farrand, Stephen K., 547Favre, Ren6e, 1069Ferron, W., 62Filip, Camille C., 443Fleet, Graham H., 207Foglesong, M. A., 325Friedman, M., 619Fumisawa, Asako, 632Funayama, Shigehiro, 1000

Geisler, Jarmila, 661Georgopoulos, S. G., 334Gerrard, T. L., 1057Gibson, D. T., 879, 923, 930Gilpin, R. W., 672Goldschmidt, Eugene P., 508Golub, Ellis E., 840Gordon, Milton P., 547Gotschlich, E. C., 250Gough, Michael, 508Grutsch, James, 650Guerry, Patricia, 144Guinea, J., 9Gustafson, Ralph A., 443Guterman, Sonia K., 638

Haag, George, 386Hagen, Per-Otto, 643Hall, B. D., 619Hall, Ruth M., 1063Hancock, Robert E. W., 726Hanson, R. S., 70Hardcastle, Ronald V., 294Hash, John H., 913

i

Hashimoto, Seiji, 811Helinski, D. R., 450Hernandez, S., 9Hill, Ruth F., 33Holmes, W. M., 220Hooper, Alan B., 899Hopkins, Donald L., 612Hopper, Anita K., 619, 952Horowitz, A., 36Howell, Neil, 1063Hranueli, K., 684Hug, Daniel H., 92Hunter, John K., 92Hutchinson, Keith W., 70

laccarino, Maurizio, 1069Inouye, Masayori, 907Inselburg, Joseph, 478, 649luchi, Shiro, 632

Jeng, Yun-Hua, 514Joenje, H., 784Jofre, J., 9Johansen, Ivar, 522Johnson, Mary K., 844Johnston, K. H., 250Jones, Richard F., 889, 893Jorstad, Caroline M., 857Jousimies, Hannele, 753

Kadner, Robert J., 401Kamekura, Masahiro, 339Kane, J. F., 220Kappas, A., 334Katz, Leonard, 450Kemper, Jost, 937Kessin, Richard H., 776Khachatourians, George G795

Kinghorn, J. R., 534Komano, Tohru, 811Konings, W. N., 784Konisky, Jordan, 579Kooistra, Jan, 705Konrad, Michael W., 228Koshland, D. E., Jr., 640Kottel, Randall H., 650Krieg, Noel R., 691Kunimoto, Setsuko, 394

Langworthy, Thomas A., 106Lastras, Mercedes, 593Laughon, Barbara E., 691Lawther, Robert P., 461Lederberg, Ester M., 1072Lengeler, J., 50

AUTHOR INDEX

Levin, Gary, 564Levinthal, Mark, 1069Liau, Deng-Fong, 913Lichstein, Herman C., 718Lin, E. C. C., 50Lindgren, Viveka, 431Linnane, Anthony W., 1063Lorkiewicz, Z., 771Lukins, H. B., 1063

McGinnis, Etheleen, 554McKay, L. L., 830Magee, P. T., 619, 952Magill, Clint W., 202Magill, Jane M., 202Mahadevan, Venkatanarayana,930

Makela, P. Helena, 753, 760, 765Mandelstam, J.,. 684Manis, Jack J., 192Manley, Mandel, 961, 976Markovetz, A. J., 325Maruo, Bunji, 82, 410, 416, 425Marzluf, George A., 371Matsuda, Futami, 117Matsumoto, Kazuko, 632Matsumoto, Kouji, 666Mayberry, William R., 106Mayer, H., 760, 765Melly, M. Ann, 913Merrick, J. M., 152Michaels, G. E., 992Miller, Sara E., 992Mollenhauer, Hilton H., 612Morris, David R., 857Morris, Howard, 583Mortlock, R. P., 162, 170, 176Mowshowitz, Solomon, 363Mufloz, Emilio, 593Murofushi, Etsuyo, 117Murray, R. G. E., 303Murray, R. G. E., 1019, 1039

Nagata, Yoshiho, 410, 416, 425Naka, Machiko, 117Nakamura, Hanh T., 57Narrod, S., 672Neidhardt, Frederick C., 736Nester, Eugene W., 547Nestmann, Earle R., 33Neter, E., 760Newell, Peter C., 776Nojima, Shoshichi, 543

O'Brien, R. W., 661Okamoto, Nakako, 543Oliver, Daphna R., 192Onishi, Hiroshi, 339Orlowski, Michael, 650Osborn, M. J., 138

Pachas, Willy N., 576

Page, W. J., 44Pares, R., 9Parisi, B., 821Patel, Nanu, 220Patel, T. R., 879Pateman, J. A., 534Patrick, Michael A., 76Paul, Aniko V., 907Pauling, Crellin, 861Pedrosa, Fibio O., 336Pemberton, J. M., 748Phaff, Herman J., 207Philipson, Lennart, 19Piggot, P. J., 684Pinkett, M. Olivia, 345Pirnik, M. P., 868Powell, Jerry S., 547Prasad, Chandan, 805

Reeve, John N., 560Reeves, Peter, 726Reiner, Albey M., 183Richardson, Charles C., 494Rick, Paul D., 138Riemer, Elaine, 461Rogers, P. J., 282, 653Rogerson, Allen C., 332Rogolsky, Marvin, 57Ruch, F. E., 50Rud6, J. M., 123Russa, R., 771Rutberg, Lars, 431

Saenko, A. S., 1St. John, Richard T., 266Saito, Hiuga, 666Sakai, Hiroshi, 811Sala, F., 821San-Blas, Gioconda, 602Scherer, Gunther, 386Schlesinger, Milton J., 583Schnepf, E., 330Shah, Vinod K., 266Shaw, Charles R., 976Shibata, Takehiko, 666Shilo, Moshe, 635Shineberg, B., 500Shizuya, Hiroaki, 494Shmueli, E., 36Singer, Richard A., 677Singh, A. P., 129Sj6str6m, Jan-Eric, 19Skurray, Ronald A., 726Smirnov, G. B., 1Smith, David F., 736Smith, J. T., 351Smith, Paul F., 176Spencer, Roberto R., 202Spurlock, Ben O., 992Steimer-Veale, Kathelyn, 848Stewart, P. R., 282, 653

Stimler, Norma P., 138Stinson, M. W., 152Stock, J. J., 44Stone, Jane E., 332Streckfuss, J. L., 1061Sugarman, Laurence I., 242Summers, Anne O., 249Szaniszlo, Paul J., 294

Takeuchi, Tomio, 394Tanaka, Shuji, 632Taylor, Barry L., 640Taylor, Craig D., 98Taylor, Mary L., 98Taylor, W. Herman, 98Telford, J. N., 1057Terry, Kathleen R., 899Tevethia, M. J., 961, 976Tipper, Donald J., 795Tokuno, Shin-Ichi, 508Tsunematsu, Yukinori, 117Tu, J. C., 986Tyson, Greta E., 629

Uehara, Hiroshi, 82Umezawa, Hamao, 394

Valoix, Josefina, 9Varon, Mazal, 635Venema, Gerard, 705, 784Vogel, J. J., 1061

Walker, James R., 443Watson, William J., 401Weare, N. M., 258Webb, R. B., 62Welch, S. K., 619Wesseling, Anne C., 893Whang, H. Y., 760White, David, 650Whitfield, Harvey J., 192Williams, Ann C., 554Williams, H. H., 1057Williams, Keith L., 776Williams, L. S., 554Wolfinbarger, L., Jr., 371Wong, W., 672Wright, Andrew, 638

Yagil, Ezra, 583Yamaguchi, Kazuo, 410, 416,425

Yamane, Kunio, 82Yoakum, G., 62Yoneda, Yuko, 82Yoshikawa, Masanosuke, 117Yosuv, D., 36Young, F. E., 672

Zancan, Glaci T., 336, 1000

* .

J. BACTERIOL.

SUBJECT INDEX

VOLUME 119

Acinetobacterfatty acids, 76hydrocarbon influence on fatty acids, 76

Adenosine transportN. crassa, 202

Adenosine triphosphatesporulation in B. subtilis, 70

Adenyl cyclase, histochemical localization ofrelationship between membrane envelopes andplasma membranes, 986

Aerobacter cloacaecitrate metabolism, 661

Agrobacterium tumefaciens and phage PS8DNA, 547

L-Alanine metabolismspore germination, 805

Alkaline phosphataseE. coli, 583regulatory genes, 583

Amino acid dehydrogenasesM. xanthus, 650

Aminoacyl-transfer ribonucleic acid synthetasesfor branched-chain amino acids

E. coli, 554Anacystis nidulansdark endogenous metabolism, 677

Anthranilate synthase complexesBacillus species, 220

Aquaspirillum gracilesugar catabolism, 691

L-Arabinose metabolismR. japonicum, 336

D-Arabinose-5-phosphate transportS. typhimurium, 138

D-Arabitol catabolic pathwayK. aerogenes, 170, 176

Artemia, spirochete-infectedrenal lesion, 629

Aspergillu8 nidulansL-glutamate transport, 534griseofulvin treatment, 334interference with chromosome segregation, 334

Autolysispreferential release of old cell walls, 672S. aureus, 672

Bacillus circulan8 glucanasesS. pombe cell walls, 207

Bacillus megateriumcalcium uptake in, 840

Bacillus papilliaetrehalase metabolism by, 484

Bacillus speciesanthranilate synthase complexes, 220

Bacillus subtilisL-alanine metabolism, inhibition of, 805a-amylase mutants, 416

iii

a-amylase synthesis, 410cell-bound a-amylase in, 425cell surface, growth, 560DNA-membrane interactions, 784glycerol metabolism, 431mapping by 5-bromouracil sensitization to UV

inactivation, 666mRNA of spores, 514operons specific for sporulation, 684peptide chain initiation and elongation, 821protease productivity, regulation of, 82protein (altered) with triosephosphate isomer-

ase activity, 976protein synthesis, 821spore germination, 805spore mRNA, 514sporulation, inhibition of, 57, 70suppressor mutations, 961thymineless death, 36

Bacteriophagechanneling toward lysogenization, 508

Bacteriophage deoxyribonucleic acidradiation-induced breaks, 522

Bacteriophage 411 genomeS. aureus competence, 19

Bacteriophage PS8 and Agrobacterium tumefaciensDNA, 547

Bacteriophage-like particlesF. symbiosum, 325

Bdellovibriomutants, 635

l-ft' subunits of ribonucleic acid polymeraseE. coli minicells, 332

Biosynthesisa factor in S. cerevisiae, 386

Biotin uptakeS. cerevisiae, 718

Brevibacterium erythrogeneshydrocarbon metabolism by, 868

5-Bromouracil sensitization to ultraviolet inac-tivation

B. subtilis mapping, 666

CalcificationE. coli, 1061

Calciumuptake in B. megaterium, 840

Candida albicans chlamydosporeselectron microscopy, 992

,8-Carotene biogenesisM. kansasii, 527

Cell divisionantibiotic treatment, 303

Cell surface, growth ofB. 8ubtili8, 560

Cell wallH. farciminosum forms, 602

SUBJECT INDEX

Cell wall superficial macromolecular arraysS. putridiconchylium, 1019

Chitin synthesispolyoxin D, 564S. cerevisiae, 564

ChlamydosporesC. albicans, 992

Chromosome sizeP. aerugina8a, 748

Citrate metabolismA. cloacae, 661

Citrobacter intermediumamino acid excretion, 9episomic element, 9fermentative metabolism, 9

Cladosporium werneckiidimorphism, 294

Colicin plasmidsincompatibility of El, E2, and E3 in E. coli, 478Mu phage infection, 469replication in, 450

Common antigenSalmonella groups B and C,, 765S. minnesota R mutants, 760

Common antigen determinationLPS genes, 760, 765

CompetenceS. aureus, 19

Composite plasmidS. typhimurium, 192

Conditional lethalityE. coli mutant, 1reversion to viability, 1

Conjugation-defective femalesE. coli, 183

Conjugation-defective mutantsE. coli, 726

Con- mutantsE. coli, 726

"Constrictive" modes of divisionE. coli, 303

Continuous cultureM. genevenfis, 282oxygen and glucose effects, 282

Dark endogenous metabolismA. nidulans, 677mutational analysis, 677

Deoxyribonucleic acidA. tumefaciens and phage PS8, 547H. influenzae rec-, 705

Deoxyribonucleic acid-membrane interactionsB. 8ubtilis, 784

Deoxyribonucleic acid polymerase IIpolB mutation, 494

Deoxyribonucleic acid replicationE. coli mutant, 811

Detergent-resistant phospholipase A locusmapping, 543

Dictyostelium di8coidcumlinkage analysis, 776

Dicyclohexylcarbodiimide treatmentE. coli mutants, 129

Dihydroorotate dehydrogenaseL. bulgaricus, 98

DimorphismC. werneckii, 294

Enterobacteriaculture medium, 736

Enzyme secretion, extracellularP. lemoignei, 152

Episomic elementamino acid excretion, 9C. intermedium, 9fermentative metabolism, 9

Erysipelothrix rhusipathiaeL-forms, 576

Escherichia colialkaline phosphatase, 583aminoacyl-tRNA synthetases for branched-chain amino acids, 554

calcification, 1061c-AMP stimulation, 450cell division regulation, 443colicin plasmids, 478conditional lethality, 1conjugation-defective females, 183conjugation-defective mutants, 726dicyclohexylcarbodiimide treatment, 129dnaD mutant, 840DNA replication in mutants, 811Hg(II)-reducing activity, 242ilvA, complementation between different muta-

tions, 1069lactose repressor, inactivation of, 500macromolecular composition, 270methionine transport in, 401methylglyoxal accumulation, 357mRNA synthesis, inhibition of, 795mutagenesis by mutator gene mutHl, 33mutant tolerant to colicins Ia and Ib, 379pIdA mapping, 543polB locus, 494polypeptide chain chain elongation, 857preirradiation ribonucleic acid synthesis inhibi-

tion, 123protein synthesis, abnormal, 345replication gap closure, inhibition of, 62selenium deposits, 1057septum formation, 303, 1039thiolutin, 795thymineless mutagenesis, 861

Escherichia coli minicellst-ft' subunits of RNA polymerase, 332

Ethidium bromideB. 8ubtilis sporulation, 57sporulation, inhibition of, 57

Fatty acidsAcinetobacter, 76hydrocarbon influence, 76R. trifolii LPS, 771

Flagellar rotation, reversalP. citronellolis, 640S. typhimurium, 640

Fusobacterium symbio8umbacteriophage-like particles, 325

iV J. BAc=RoL.

SUBJECT INDEX

D-Galactose degradationS. aureu8, 698D-tagatose 6-phosphate pathway, 698

Geotrichum candidum septum or valva of centricdiatom, 330

Gluconobacter cerinusfructose 5-dehydrogenase-negative mutant of363

L-Glutamate transportA. nidulans, 534

Glycerol catabolismK. aerogene8, 50

Glycerol diether lipidsS. acidocaldarius, 106

GlycoproteinsM. gypseum, 44

GriseofulinA. nidulans chromosome segregation, 334

Haemophilus influenzaeDNA in transformation-deficient strains, 705

Hg(II)-reducing activityE. coli, 242

Histidine ammonia-lyaseP. putida, 92temperature effects, 92

Histoplasma farciminosumcell wall of yeastlike and mycelial forms, 602

HydrocarbonsAcinetobacter, 76fatty acids, 76

ilvAcomplementation between different mutations,

1006E. coli, 1006

Klebsiella aerogenesD-arabitol catabolic pathway, 170, 176glycerol catabolism, 50pentitol genetics, 176ribitol catabolic pathway, 162, 176

Klebsiella pneumoniaenitrogenase synthesis regulation, 266

,g-Lactamaseoxacillin-hydrolyzing, 351R-factor-mediated, 351

LactobaciUus bulgaricusdihydroorotate dehydrogenase, 98

Lactose degradationS. aureus, 698D-tagatose 6-phosphate pathway, 698

Lactose repressorsE. coli, 500inactivation, 500

leuD geneS. typhimurium, 937

leuD gene, substitution for, 937L-formE. rhusiopathiae, 576

Linkage analysisD. di8coideum, 776

Lipophilic proteinsS. cerevisiae mitochondria, 653

Lipopolysaccharide core biosynthesisgenetic analysis, 753S. minnesota R mutants, 753

Lipopolysaccharide genescommon antigen determination, 760, 765

Lysis of yeast cell wallsB. circulans glucanases, 207

Lysogenizationchanneling phage, 508

Macromolecular compositionE. coli, 270

Macromoblecule synthesis and breakdownyeast, 619

Mapping, genetic5-bromouracil sensitization to UV inactivation,666

B. subtilis, 666Membrane adenosine triphosphataseM. lysodeikticus, 593

Membrane envelopes and plasma membranesrelationship, 986

Membrane vesiclesB. subtilis, 784DNA interaction, 784

Messenger ribonucleic acidB. 8ubtilis spores, 514spores, 514

Messenger ribonucleic acid synthesisE. coli, 795inhibition, 795thiolutin, 795

MetabolismB. erythrogene8, 868glycerol in B. 8ubtili8, 431Pseudomonas, 923, 930trehalose by B. papilliae, 484

Methylglyoxalaccumulation by E. coli K-12, 357

Mg2+M. lysodeikticu8 membrane ATPase, 593

Micrococcus lysodeikticusMg'+ treatment, 593

Micrococcus varianshalophilic nuclease from, 339

Microsporum gyp8eumglycoproteins, 44sporulation, 44

Mitochondrial genetic crossespolarity alleles, 1063

Mitochondrial protein-synthesizing systemS. cerevisiae, 653

Mucor geneven8iscontinuous culture, 282yeast-mycelium conversion, 282, 294

MutagenesisE. coli, 33, 861mutator gene mutH1, 33

Mutator mutationP22 and R factor genes, 638S. typhimurium, 638

VOL. 119, 1974 v

SUBJECT INDEX

Mycobacterium karsasiif-carotene biogenesis, 527

Myxococcus xanthusamino acid dehydrogenases, 650transaminases, 650

Near-ultraviolet light photoproducts of L-trypto-phan

replication gap closure, inhibition of, 62Neisseria gonorrhoeae

outer membrane, 250Neurospora crassaadenosine transport and growth inhibition, 202cross-pathway regulation in, 889, 893peptide utilization by, 371

Nitrogenase activityP. boryanum, 258

Nitrogenase synthesisK. pneumoniae, 266regulation by oxygen, 266

Nitrosomonasphotoinactivation of ammonia oxidation in, 899

Nocardia erythropolisrecombinant recovery, 646

Nucleaseof halophilic M. varians, 339

OperonsB. subtilis sporulation, 684statistical estimate, 684

Orthophosphate requirementphosphoenolpyruvate formation from pyruvate,

1066Outer membraneN. gonorrhoeae, 250

Outer membrane and cytoplasmic membranePseudomonas, 1000

Pentitol geneticsK. aerogenes, 176

Peptidasein S. pneumoniae, 844

Phosphate incorporation into tRNA nucleosidetriphosphate termini, 228

Phosphoenolpyruvate-dependent phosphotrans-ferase system

enrichment of mutants, 632Phosphoenolpyruvate formationorthophosphate requirement, 1066

PhotosynthesisP. boryanum, 258

Pierce's disease bacterium, 612Plasmalogen

S. ridiculosis lipids, 643Plasma membranes and membrane envelopes

relationship, 986Plasmid deoxyribonucleic acid

S. typhimurium transformation, 1072Plectonema boryanum

nitrogenase activity, 258photosynthesis, 258

Pleiotropic alterationS. aureus toxins and enzymes, 117

pidA mappingE. coli, 543

Polarity allelesmitochondrial genetic crosses, 1063

polAr uvrPrreversion to viability, 1

polB locusDNA polymerase II, 494E. crli, 494

Poly-fl-hydroxybutyric acid depolymeraseP. lemoignei, 152

Polyol dialkyl glycerol triether lipidsS. acidocaldarius, 106

Polyporus circinatuspyridine nucleotide-independent L-lactate de-

hydrogenase, 1000Polyoxin D

chitin synthesis, 564S. cerevisiae, 564septum formation, 564

Potassium ionsprotein synthesis, 821

Preirradiation ribonucleic acid synthesis inhibi-tion

E. coli, 123Protease productivityB. subtilis, 82

Proteins, alteredB. subtilis, 976triosephosphate isomerase activity, 976

Protein synthesisB. 8ubtilis, 821E. coli mutant, 345potassium ions, 821

Protein synthesis in relation to sporulation andmeiosis in yeast, 952

Pseudomonasmetabolism in, 923outer membrane and cytoplasmic membrane,

1006Pseudomonas aeruginosachromosome size, 748

Pseudomonas citronellolisflagellar rotation, reversal, 640

Pseudomonas lemoigneiextracellular enzyme secretion, 152poly-,f-hydroxybutyric acid depolymerase, 152

Pseudomonas putidahistidine ammonia-lyase, 92metabolism in, 930(+ )-cis-naphthalene dihydriol dehydrogenase

of, 879Psychophilesheat stability, 92

Pyridine nucleotide-independent L-lactate de-hydrogenase

P. circinatus, 1000

R62, 144Radiation-induced breaksphage X DNA, 522

Recombinant recoveryN. erythropolis, 646

Renal lesionspirochete-infected Artemia, 629

Replication gap closure, inhibition ofE. coli, 62

ni J. BAcTmuOL.

SUBJECT INDEX

R factor R62, 144Rhizobium japonicumRhizobium trifolii lipopolysaccharides

fatty acids, 717L-arabinose metabolism, 336

Ribitol catabolic pathwayK. aerogenes, 162, 176

R plasmid R62, 144

Saccharomycesprotein synthesis in relation to sporulation and

meiosis, 952Saccharomyces cerevisiaea factor biosynthesis in, 386allantoin degradation in, 461biotin uptake, 718chitin synthesis, 564lipophilic proteins of mitochondria, 653mitochondrial protein-synthesizing system, 653polyoxin D treatment, 564septum formation, 564

Salmonella minnesota R mutantsgenetic analysis, 753, 760, 765LPS core biosynthesis, 753

Salmonella typhimuriumD-arabinose-5-phosphate transport, 138channeling phage toward lysogenization, 508composite plasmid, 192flagellar rotation, reversal, 640mutator mutation, 638P22 and R factor genes, 638D-sedoheptulose-7-phosphate transport, 138strains sensitive and resistant to methionine

sulfoximine, 848sugar phosphate transport, 138transformation by plasmic DNA, 1072

Schizosaccharomyces pombeB. circulans glucanases, 207lysis of cell wall, 207

D-Sedoheptulose-7-phosphate transportS. typhimurium, 138

Selenium depositsE. coli, 1057

Septum formationE. coli, 303, 1039polyoxin D, 564S. cerevisiae, 564

SialidasesStreptomyces, 394

Sphaerophorus ridiculosis lipidsplasmologen, 643

Spirillum putridiconchyliumcell wall superficial macromolecular arrays,

1019Spirochete-infected Artemia

renal lesion, 629Spore germination

L-alanine metabolism, inhibition of, 805B. subtilis, 805

Sporulationadenine nucleotide changes, 70

B. subtilis, 57, 70ethidium bromide inhibition of, 57initiation of, 70

Staphylococcus aureusautolysis, 672bacteriophage 11 genome, 19competence, 19pleiotropic alteration, 117surface polysaccharide from, 913

Streptococcus lactislactose-negative mutants of, 830

Streptococcus pneumoniaepeptidases of, 844

Streptomycessialidases of, 394

Sugar catabolismA. gracile, 691

Sugar phosphate transportS. typhimurium, 138

Sulfolobus acidocaldariuslipids, 106

Suppressor mutantsB. subtilis, 961

supQ geneleuD substitute, 937

supQ mutationscharacterization, 937S. typhimurium, 937

D-Tagatose 6-phosphate pathway of lactose andD-galactose degradation, 698

ThiolutinE. coli, 795mRNA synthesis, inhibition of, 795

Thymineless deathB. subtilis, 36

TransaminasesM. xanthus, 650

Transcriptional unitlength, average, 228

Transformationplasmid DNA, 1072S. typhimurium, 1072

Ultraviolet resistanceE. coli, 123Preirradiation treatment, 123

Valva of centric diatom or septum of Geotrichumcandidum, 330

Vibrio parahaemolyticusenrichment of mutants, 632

Yeastmacromolecule synthesis and breakdown, 619protein synthesis in relation to sporulation and

meiosis, 952Yeast-mycelium conversion

reversibility, 282

VOL. 119, 1974 *ii

JOURNAL

OF

BACTERIOLOGY

VOLUME 119

WASHINGTON, D.C. 20006

1974

JOURNAL OF BACTERIOLOGYVOLUME 119 * 1974

EDITORIAL BOARD

L. LEON CAMPBELL, Editor-in-Chief (1975)University of Delaware, Newark

R. L. ANDERSON, Editor (1975)Michigan State University, East Lansing

S. G. BRADLEY, Editor (1979)Virginia Commonwealth University, Richmond

ROY CURTISS III, Editor (1979)University of Alabama, BirminghamCARL SCHNAITMAN, Editor (1978)University of Virginia, CharlottesvilleH. E. UMBARGER, Editor (1976)Purdue University, Lafayette, Ind.

James N. Adams (1976)Arthur 1. Aronson (1976)Stephen D. Barbour (1976)W. Lane Barksdale (1975)M. E. Bayer (1975)Blaine Beaman (1976)Claire M. Berg (1976)Richard S. Berk (1974)Winfried Boos (1976)Herbert W. Bover (1975)Jean Brenchley (1977)B. Wesley Catlin (1974)D. Joseph Clark (1974)Stanely N. Cohen (1976)Ronald S. Cole (1974)S. F. Conti (1975)Nicholas R. Cozzarelli (1975)Walter J. Debrogosz (1976)R. G. Eagon (1974)Alan D. Elbein (1976)Wolfgang, Epstein (1974)Stanley Falkow (1975)Gerald R. Fink (1975)W. R. Finnerty (1976)A. T. Ganesan (1975)

Howard Goldfine (1975)Harry Gooder (1975)Joseph S. Gots (1975)Harlyn 0. Halvorson (1975)George Hegeman (1974)Donald R. Helinski (1975)Charles E. Helmstetter (1975)Joseph T. Holden (1975)Stanley C. Holt (1974)H. Ronald Kaback (1975)Sam Kaplan (L974)David E. Kennell (1974)Robert L. Lester (1975)Hillel S. Levinson (1975)E. C. C. Lin (1975)K. Brooks Low (1976)Elizabeth McFall (1976)Michael H. Malamy (1975)Manley Mandel (1975)David Mardon (1974)Robert E. Marquis (1976)Neil H. Mendelson (1975)M. L. Morse (1975)Eugene W. Nester (1975)Richard P. Novick (1976)

L. Nicholas Ornston (1975)Leo Parks (1976)Martin Pato (1975)Paul V. Phibbs (1976)Allen T. Phillips (1976)Lewis 1. Pizer (1975)Jack Preiss (1974)Charles C. Remsen III (1976)William S. Reznikoff (1976)Antonio H. Romano (1976)Harold L. Sadoff (1974)M. R. J. Salton (1975)David Schlessinger (1974)Irwin H. Segel (1976)Jane K. Setlow (1975)Helen Jean Shadomy (1975)Stanley K. Shapiro (1976)Simon Silver (1976)W. R. Sistrom (1975)Lucile Smith (1975)Donald J. Tipper (1975)Arthur Weissbach (1974)Clifford A. Woolfolk (1976)Stanley A. Zahler (1974)Howard Zalkin (1976)

ROBERT A. DAY, Managing Editor, 1913 I St., N. W., Washington, D.C. 20006

EX OFFICIOPhilipp Gerhardt, President' (1974-1975)

J. Mehsen Joseph, Secretary

The Journal ofBacteriology, a publication of the American Society forMicrobiology. 1913 St., N.W., Washington. D.C. 20006, is devoted to theadvancement and dissemination of fundamental knowledge concerningbacteria and other microorganisms. The Journal is published monthly, andthe twelve numbers are divided into four volumes per year. The subscrip-tion price is $85 (Postal Union Countries, $87: other foreign, $88) per year.Single copies are $8.00 (foreign, 8.25). Members of the American Societyfor Microbiology may receive the Journal as part of their dues. Correspon-dence relating to subscriptions, reprints, defective copies, availability of backissues, lost or late proofs, disposition of submitted manuscripts, and gen-eral editorial matters should be directed to the ASM Publications Office.1913 St., N.W., Washington, D.C. 20006 (area 202 833-9416). Correspon-dence from ASM members relating to membership dues, member subscrip-

Helen R. Whiteley, Vice-President (1974-1975)T. J. Carski, Treasurer

tions, changes of address, incorrect journals, etc., should be directed to theExecutive Secretary, American Society for Microbiology, 1913 1 St., N.W.,Washington, D.C. 20006. Published monthly by the ASM at 1913 1 St.,N.W., Washington, D.C. 20006.

Second class postage paid at Washington, D.C. 20006, and at additionalmailing offices.Made in the United States of America.Copyright 0 1974, American Society for Microbiology.All Rights Reserved.

Volume 119 Contents for July Number 1

Morphology and UltrastructureIsolation and Characterization of the Outer Membrane of Neisseria gonorrhoeae.

K. H. JOHNSTON AND E. C. GOTSCHLICH................................... 250Effects of Oxygen and Glucose on Energy Metabolism and Dimorphism of Mucor

genevensis Grown in Continuous Culture: Reversibility of Yeast-Mycelium Con-version. P. J. ROGERS, D. D. CLARK-WALKER, AND P. R. STEWART............ 282

Characterization of Dimorphism in Cladosporium werneckii. RONALD V. HARDCASTLEAND PAUL J. SZANISZLO................................................. 294

Septum Formation in Escherichia coli: Characterization of Septal Structure and theEffects of Antibiotics on Cell Division. I. D. J. BURDETT AND R. G. E. MURRAY ..303

Morphology of Bacteriophage-Like Particles from Fusobacterium symbiosum. M. A.FOGLESONG AND A. J. MARKOVETZ....................................... 325

Septum of Geotrichum candidum or Valva of a Centric Diatom? E. SCHNEPF ........ 330

Genetics and Molecular BiologyGenetic Analysis of a Temperature-Resistant Revertant of the Conditional Lethal

Escherichia coli Double Mutant polAl2 uvrE502. G. B. SMIRNOV ANDA. S. SAENKO........................................................... 1

A New Episomic Element Controlling Fermentative Metabolism and Excretion ofAmino Acids by Citrobacter intermedium C3. R. PARES, J. GUINEA, S. HERNANDEZ,JOSEFINA VALOIX, AND J. JOFRE ......................................... 9

Role of the i11 Phage Genome in Competence in Staphylococcus aureus. JAN-ERICSJOSTROM AND LENNART PHILIPSON........................................ 19

Mutagenesis by Mutator Gene mutHl in Continuous Cultures of Escherichia coli.EARLE R. NESTMANN AND RUTH F. HILL.................................. 33

Thymineless Death in Bacillus subtilis: Correlation Between Cell Lysis and Deoxy-ribonucleic Acid Breakdown. E. EPHRATI-ELIZUR, D. Yosuv, E. SHMUELI, ANDA. HOROWITZ........................................................... 36

Inhibition of Replication Gap Closure in Escherichia coli by Near-Ultraviolet LightPhotoproducts of L-Tryptophan. G. YOAKUM, W. FERRON, A. EISENSTARK, ANDR. B. WEBB............................................................ 62

Effect of Preirradiation Ribonucleic Acid Synthesis Inhibition on Resistance to Ultra-violet Light with Resistant and Sensitive Strains of Escherichia coli B/r. J. M.RUD19 AND C. 0. DOUDNEY ............................................... 123

Effect of Dicyclohexylcarbodiimide on Growth and Membrane-Mediated Processesin Wild Type and Heptose-Deficient Mutants of Escherichia coli K-12. A. P.SINGH AND P. D. BRAGG................................................. 129

R62, a Naturally Occurring Hybrid R Plasmid. PATRICIA GUERRY, STANLEY FALKOW,AND NAOMI DATTA..................................... I ............... 144

Escherichia coli Females Defective in Conjugation and in Adsorption of a Single-Stranded Deoxyribonucleic Acid Phage. ALBEY M. REINER.................... 183

Evidence for a Composite State of an F'his,gnd Element and a Cryptic Plasmid in aDerivative of Salmonella typhimurium LT2. DAPHNA R. OLIVER, JACK J. MANIS,AND HARVEY J. WHITFIELD............................................... 192

Apparent Average Length of the Transcriptional Unit in Bacteria. MICHAEL W. KON-RAD . . . . . . . . . .. . . . . . .. .. . . . . . . . . . . . . . . . . . . . . 228

Cell-Free Mercury(II)-Reducing Activity in a Plasmid-Bearing Strain of Escherichiacoli. ANNE 0. SUMMERS AND LAURENCE I. SUGARMAN........................ 242

3,8-1' Subunits of Ribonucleic Acid Polymerase in Episome-Free Minicells of Escherichiacoli. ALLEN C. ROGERSON AND JANE E. STONE.............................. 332

Interference of Griseofulvin with the Segregation of Chromosomes at Mitosis inDiploid Aspergillus nidulans. A. KAPPAS AND S. G. GEORGOPOULOS............ 334

Physiology and MetabolismChanges in Microsporum gypseum Mycelial Wall and Spore Coat Glycoproteins Dur-

ing Sporulation and Spore Germination. W. J. PAGE AND J. J. STOCK............ 44xi

Regulation of Glycerol Catabolism in Klebsiella aerogenes. F. E. RUCH, J. LENGELER,AND E. C. C. LIN........................................................ 50

Sensitivity of an Early Step in the Sporulation of Bacillus subtilis to Selective Inhibi-tion by Ethidium Bromide. MARVIN ROGOLSKY AND HANH T. NAKAMURA....... 57

Adenine Nucleotide Changes Associated with the Initiation of Sporulation in Bacillussubtilis. KEITH W. HUTCHISON AND R. S. HANSON ........................... 70

Influence of Hydrocarbons and Derivatives on the Polar Lipid Fatty Acids of anAcinetobacter Isolate. MICHAEL A. PATRICK AND PATRICK R. DUGAN............ 76

Effect of Temperature on Histidine Ammonia-Lyase from a Psychrophile, Pseu-domonas putida. DANIEL H. HUG AND JOHN K. HUNTER..................... 92

Long-Chain Glycerol Diether and Polyol Dialkyl Glycerol Triether Lipids of Sulfolobusacidocaldarius. THOMAS A. LANGWORTHY, WILLIAM R. MAYBERRY, AND PAUL F.SMITH............................................................... 106

Pleiotropic Alteration of Activities of Several Toxins and Enzymes in Mutants ofStaphylococcus aureus. MASANOSUKE YOSHIKAWA, FUTAMI MATSUDA, MACHIKONAKA, ETSUYO MUROFUSHI, AND YUKINORI TSUNEMATSU.................... 117

Transport of D-Arabinose-5-Phosphate and D-Sedoheptulose-7-Phosphate by theHexose Phosphate Transport System of Salmonella typhimurium. LEON EIDELS,PAUL D. RICK, NORMA P. STIMLER, AND M. J. OSBORN...................... 138

Ribitol Catabolic Pathway in Klebsiella aerogenes. W. T. CHARNETZKY AND R. P.MORTLOCK............................................................. 162

D-Arabitol Catabolic Pathway in Klebsiella aerogenes. W. T. CHARNETZKY AND R. P.MORTLOCK............................................................ 170

Close Genetic Linkage of the Determinants of the Ribitol and D-Arabitol CatabolicPathways in Klebsiella aerogenes. W. T. CHARNETZKY AND R. P. MORTLOCK...... 176

Relationship Between [8-14C]Adenosine Transport and Growth Inhibition in Neuro-spora crassa Strain ad-8. JANE M. MAGILL, ROBERTO R. SPENCER, AND CLINT W.MAGILL.................................................... ........... 202

Nitrogenase Activity and Photosynthesis in Plectonema boryanum. N. M. WEARE ANDJOHN R. BENEMANN..................................................... 258

Regulation of Nitrogenase Synthesis by Oxygen in Klebsiella pneumoniae. RICHARDT. ST. JOHN, VINOD K. SHAH, AND WINSTON J. BRILL.........................266

Macromolecular Composition During Steady-State Growth of Escherichia coli B/r.PATRICK P. DENNIS AND HANS BREMER................................... 270

L-Arabinose Metabolism in Rhizobium japonicum. FABIO 0. PEDROSA AND GLACI T.ZANCAN................................................................ 336

EnzymologyRegulation of Neutral Protease Productivity in Bacillus subtilis: Transformation of

High Protease Productivity. HIROSHI UEHARA, YUKO YONEDA, KUNIO YAMANE,AND BUNJI MARUO...................................................... 82

Biosynthetic Dihydroorotate Dehydrogenase from Lactobacillus bulgaricus: PartialCharacterization of the Enzyme. W. HERMAN TAYLOR, CRAIG D. TAYLOR, ANDMARY L. TAYLOR....................................................... 98

Extracellular Enzyme Secretion by Pseudomonas lemoignei. M. W. STINSON AND J. M.MERRICK.............................................................. 152

Lysis of Yeast Cell Walls: Glucanases from Bacillus circulans WL-12. GRAHAM H.FLEET AND HERMAN J. PHAFF......................................... 207

Homologous and Hybrid Complexes of Anthranilate Synthase from Bacillus Species.NANU PATEL, W. M. HOLMES, AND J. F. KANE............................. 220

*Xi CONTENTS

Volume 119 Contents for August Number 2Morphology and Ultrastructure

Growth of the Bacillus subtilis Cell Surface. JOHN N. REEVE...................... 560L-Form Induction, Morphology, and Development in Two Related Strains of Ery-

sipelothrix rhusiopathiae. WILLY N. PACHAS AND VICTORIA R. CuRRID .......... 576Chemical and Ultrastructural Studies on the Cell Walls of the Yeastlike and Mycelial

Forms of Histoplasma farciminosum. GIOCONDA SAN-BLAS AND LUIS M. CAR-BONELL ....................................................... . ..................602

UltrastructuralStudy of Pierce's Disease Bacterium in Grape Xylem Tissue. HILTONH. MOLLENHAUER AND DONALD L. HOPKINS................................ 612

Distinctive Renal Lesion of Spirochete-Infected Brine Shrimp. GRETA E. TYsON.... 629Genetics and Molecular Biology

Streptomycin-Induced Synthesis of Abnormal Protein in an Escherichia coli Mutant.M. OLIVIA PINKETT AND BARBARA BROWNSTEIN .............................. 345Isolation and Characterization of an Escherichia coli Mutant Tolerant to Colicins Ia

and Ib. JAMES CARDELLI AND JORDAN KONISKY............................. 379Methionine Transport in Escherichia coli: Physiological and Genetic Evidence for Two

Uptake Systems. ROBERT J. KADNER AND WILLIAM J. WATSON................ 401Genetic Control of the Rate of a-Amylase Synthesis in Bacillus subtilis. KAzuO

YAMAGUCHI, YOSHIHo NAGATA, AND BUNJI MARUO......................... 410Isolation of Mutants Defective in a-Amylase from Bacillus subtilis: Genetic Analyses.

KAzuO YAMAGUCHI, YOSHIHo NAGATA, AND BUNJI MARUO .................. 416Genetic and Biochemical Studies on Cell-Bound a-Amylase in Bacillus subtilis

Marburg. YOSHIHo NAGATA, KAZUO YAMAGUCHI, AND BUNJI MARUO......... 425Glycerol Metabolism in Bacillus subtilis: Gene-Enzyme Relationships. VIvEKA LIND-

GREN AND LARS RUTBERG......................... .......................431Bacterial Cell Division Regulation: Characterization of thednaH Locus of Escherichia

coli. CAMILLE C. FILIP, JANE SMITH ALLEN, RALPH A. GUSTAFSON, ROBERT G.ALLEN, AND JAMES R. WALKER........................................... 443

Effect of Inhibitors of Ribonucleic Acid and Protein Synthesis on the Cyclic AdenosineMonophosphate Stimulation of Plasmid ColEl Replication. LEONARD KATZ ANDD. R. HELINSKI ............. ............................................ 450

Clustering of the Genes for Allantoin Degradation in Saccharomyces cerevisiae. ROBERTP. LAWTHER, ELAINE RIEMER, BONNIE CHOJNACKI, AND TERRANCE G. COOPER. . 461

Isolation and Characterization of Mutants of Colicin Plasmids El and E2 After MuBacteriophage Infection. JOSEPH INSELBURG.............................. 469

Incompatibility Exhibited by Colicin Plasmids El, E2, and E3 in Escherichia coli.JOSEPH INSELBURG...................................................... 478

Mapping of a Mutation, polBlOO, Affecting Deoxyribonucleic Acid Polymerase IIin Escherichia coli K-12. JUDITH L. CAMPBELL, HIROAKI SHIZUYA, AND CHARLESC. RICHARDSON ........................................................ 494

Mutations Partially Inactivating the Lactose Repressor of Escherichia coli. B. SHINE-BERG....................................................... .......... 500

Mutant of Salmonella typhimurium That Channels Infecting Bacteriophage P22 To-ward Lysogenization. SHIN-ICHI TOKUNO, EUGENE P. GOLDSCHMIDT, ANDMICHAEL GOUGH....................................................... 508

Time Scale for Rejoining of Bacteriophage X Deoxyribonucleic Acid Molecules inSuperinfected pol+ and polAl Strains of Escherichia coli After Exposure to 4MeV Electrons. ERIK BOYE, IVAR JOHANSEN, AND TOR BRUSTAD.............. 522

Regulatory Aspects of L-Glutamate Transport in Aspergillus nidulans. J. A. PATE-MAN, J. R. KINGHORN, AND ETTA DUNN.................................... 534

Genetic Mapping of the Locus for Detergent-Resistant Phospholipase A (pldA) inEscherichia coli K-12. MIHOKO ABE, NAKAKO OKAMOTO, OSAMU DoI, AND SHO-SHICHI NOJIMA......................................................... 543

*. -

XllCONTENTS

Attempts to Detect Deoxyribonucleic Acid from Agrobacterium tumefaciens and Bac-teriophage PS8 in Crown Gall Tumors by Complementary Ribonucleic Acid/Deoxyribonucleic Acid-Filter Hybridization. FRANCINE C. EDEN, STEPHEN K.FARRAND, JERRY S. POWELL, ARNOLD J. BENDICH, MARY-DELL CHILTON,EUGENE W. NESTER, AND MILTON P. GORDON .............................. 547

Derepression of Synthesis of the Aminoacyl-Transfer Ribonucleic Acid Synthetasesfor the Branched-Chain Amino Acids of Escherichia coli. ETHELEEN MCGINNIS,ANN C. WILLIAMS, AND L. S. WILLIAMS.................................. 554

Pleiotropic Effects of Mutations Involved in the Regulation of Escherichia coli K-12Alkaline Phosphatase. HOWARD MORRIS, MILTON J. SCHLESINGER, MOSHEBRACHA, AND EzRA YAGIL............................................. 583

Macromolecule Synthesis and Breakdown in Relation to Sporulation and Meiosis inYeast. ANITA K. HOPPER, P. T. MAGEE, S. K. WELCH, M. FRIEDMAN, AND B. D.HALL.................................................................. 619

Isolation of Host-Dependent and Nonparasitic Mutants of the Facultative ParasiticBdellovibrio UKi2. MAZAL VARON, SARAH DICKBUCH, AND MOSHE SHILO...... 635

Effects of a Mutator Mutation of Salmonella typhlimurium on P22 and R Factor Genes.SONIA K. GUTERMAN AND ANDREW WRIGHT .................................638Limiting Nutrients During Mating as a Means of Increasing Recombinant Recoveryin Crosses of Nocardia erythropolis. JAMES N. ADAMS AND MARGIE M. ADAMS.... 646

Physiology and MetabolismR-Factor-Mediated,B-Lactamases That Hydrolyze Oxacillin: Evidence for Two Dis-

tinct Groups. J. W. DALE AND J. T. SMITH................................ 351Accumulation of Toxic Concentrations of Methylglyoxal by Wild-Type Escherichia

coli K-12. ROLLIE S. ACKERMAN, NICHOLAS R. COZZARELLI, AND WOLFGANGEPSTEIN............................................................... 357

Metabolic Consequences of a Block in the Synthesis of 5-Keto-D-Fructose in a Mutantof Gluconobacter cerinus. SOLOMON MOWSHOWITZ, SASHA ENGLARD, AND GADAvIGAD................................................................ 363

Peptide Utilization by Amino Acid Auxotrophs of Neurospora crassa. L. WOLFIN-BARGER, JR., AND GEORGE A. MARZLUF.................................... 371

Mechanism of a Factor Biosynthesis in Saccharomyces cerevisiae. GtNTHER SCHERER,GEORG HAAG, AND WOLFGANG DUNTZE ..................... 386

Trehalose Metabolism by Bacillus popilliae. AMARET BHUMIRATANA, RICHARD L.

ANDERSON, AND RALPH N. COSTILOW ...................... 484Messenger Ribonucleic Acid of Dormant Spores of Bacillus subtilis. YUN-HUA JENG

AND ROY H. DoI .............................. 514Biogenesis of ,8-Carotene in Mycobacterium kansasii. HUGO L. DAVID .......... 527Effect of Polyoxin D on Chitin Synthesis and Septum Formation in Saccharomyces

cerevisiae. BLAIR BOWERS, GARY LEVIN, AND ENRICO CABIB .......... . 564Membrane Adenosine Triphosphatase of Micrococcus lysodeikticus: Effect of Milli-

molar Mg2+ in Modulating the Properties of the Membrane-Bound Enzyme.MERCEDES LASTRAS AND EMILIO MUROz................................... 593

Enrichment of Mutants Lacking the Phosphoenolpyruvate-Dependent Phospho-transferase System of Vibrio parahaemolyticus by Screening with Methyl-a-D-Glucoside. KAZUKO MATSUMOTO, SHIROIUCHI, ASAKO FUMISAWA, AND SHUJITANAKA ............................................................ 632

Reversal ofFlagellar Rotation Monotrichous and Peritrichous Bacteria: Genera-tion of Changes in Direction. BARRY L. TAYLOR AND D. E. KOSHLAND, JR....... 640

Lipids of Sphaerophorus ridiculos8s: Plasmalogen Composition. PER-OTTO HAGEN.... 643

EnzymologyHalophilic Nuclease from a Moderately Halophilic Micrococcus varians. MASAHIRO

KAMEKURA AND HIROSHI ONISHI........................................ 339Purification and Characterization of Streptomyces Sialidases. SETSUKO KUNIMOTO,

TAKAAKI AOYAGI, ToMIO TAKEUCHI, AND HAMAO UMEZAWA.................. 394

X1V CONTENTS

Presence of Amino Acid Dehydrogenases and Transaminases in Myxococcus xanthusDuring Vegetative Growth and Myxospore Formation. RANDALL H. KOTTEL,MICHAEL ORLOWSKI, DAVID WHITE, AND JAMES GRUTSCH.................... 650

Errata................................................................... 652

Volume 119 Contents for September Number 3Morphology and Ultrastructure

Fatty Acids Present in the Lipopolysaccharide of Rhizobium trifolii. R. RuSSA ANDZ. LORKIEWICZ.......................................................... 771

Relationship Between the Membrane Envelope of Rhizobial Bacteroids and the PlasmaMembrane of the Host Cell as Demonstrated by Histochemical Localization ofAdenyl Cyclase. J. C. Tu................................................. 986

Electron Microscopy of Young Candida albicans Chlamydospores. SARA E. MILLER,BEN 0. SPURLOCK, AND G. E. MICHAELS.................................. 992

Superficial Macromolecular Arrays on the Cell Wall of Spirillum putridiconchylium.T. J. BEVERIDGE AND R. G. E. MURRAY................................. 1019

Electron Microscope Study of Septum Formation in Escherichia coli Strains B and B/rDuring Synchronous Growth. I. D. J. BURDETT AND R. G. E. MURRAY....... 1039

Detection of Selenium Deposits in Escherichia coli by Electron Microscopy. T. L.GERRARD, J. N. TELFORD, AND H. H. WILLIAMS.......................... 1057

Calcification by Escherichia coli. J. ENNEVER, J. J. VOGEL, AND J. L. STRECKFUSS... 1061Genetics and Molecular Biology

Genetic Mapping in Bacillus subtilis by 5-Bromouracil Sensitization to UltravioletInactivation of Transforming Activities. KOUJI MATSUMOTO, TAKEHIKO SHIBATA,AND HIUGA SAITO........................................................ 666

Mutational Analysis of Dark Endogenous Metabolism in the Blue-Green BacteriumAnacystis nidulans. W. FORD DOOLITTLE AND RICHARD A. SINGER............ 677

Statistical Estimate of the Total Number of Operons Specific for Bacillus subtilis Sporu-lation. K. HRANUELI, P. J. PIGGOT, AND J. MANDELSTAM................... 684

Fate of Donor Deoxyribonucleic Acid in a Highly Transformation-Deficient Strainof Haemophilus influenzae. JAN KOOISTRA AND GERARD VENEMA.............. 705

Con- Mutants: Class of Mutants in Escherichia coli K-12 Lacking a Major Cell WallProtein and Defective in Conjugation and Adsorption of a Bacteriophage. RONALDA. SKURRAY, ROBERT E. W. HANCOCK, AND PETER REEVES................. 726

Size of the Chromosome of Pseudomonas aeruginosa PAO. J. M. PEMBERTON. ..............748Genetic Analysis of Salmonella minnesota R Mutants with Defects in the Biosynthesis

of the Lipopolysaccharide Core. HANNELE JOUSIMIES AND P. HELENA MXKELX. 753Participation of Lipopolysaccharide Genes in the Determination of the Enterobacterial

Common Antigen: Analysis of R Mutants of Salmonella minnesota. P. HELENAMAKELA, H. MAYER, H. Y. WHANG, AND E. NETER........................ 760

Participation of Lipopolysaccharide Genes in the Determination of the EnterobacterialCommon Antigen: Analysis in Salmonella Groups B and C1. P. HELENA MXKELXAND H. MAYER.......................... 765

Linkage Analysis in Dictyostelium discoideum Using Temperature-Sensitive GrowthMutants Selected with Bromodeoxyuridine. RICHARD H. KESSIN, KEITH L.WILLIAMS, AND PETER C. NEWELL.......................... 776

Interactions Between Exogenous Deoxyribonucleic Acid and Membrane Vesicles Iso-lated from Bacillus subtilis 168. J. JOENJE, W. N. KONINGS, AND G. VENEMA. 784

Inhibition of Messenger Ribonucleic Acid Synthesis in Escherichia coli by Thiolutin.GEORGE G. KHACHATOURIANS AND DONALD J. TIPPER...................... 795

CONTENTS xv

Replication of Deoxyribonucleic Acid in Escherichia coli C Mutants Temperature-Sensitive in the Initiation of Chromosome Replication. HIROSHI SAKAI, SEIJIHASHIMOTO, AND ToHRu KOMANO......................................... 811

Protein Synthesis in Bacillus subtilis: Differential Effect of Potassium Ions on In VitroPeptide Chain Initiation and Elongation. F. SALA, M. BAZZICALUPO, AND B.PARISI.................................................................. 821

Polyamine Limitation of Growth Slows the Rate of Polypeptide Chain Elongation inEscherichia coli. CAROLINE M. JORSTAD AND DAVID R. MORRIS.............. 857

Thymineless Mutagenesis in Escherichia coli. CHARLES E. DEUTCH AND CRELLINPAULING................................................................ 861

Temperature-Sensitive Modification and Restriction Phenotypes of an Escherichia colidnaD Mutant. ANIKO V. PAUL AND MASAYORI INOUYE..................... 907

Evolution of a New Gene Substituting for the leuD Gene of Salmonella typhimurium:Characterization of supQ Mutations. JOST KEMPER.......................... 937

Protein Synthesis in Relation to Sporulation and Meiosis in Yeast. P. T. MAGEE ANDANITA K. HOPPER....................................................... 952

Pleiotropic Effects of Suppressor Mutations in Bacillus subtilis. M. J. TEVETHIA,JAMES N. BAPTIST, AND MANLEY MANDEL................................. 961

Altered Proteins with Triosephosphate Isomerase Activity in Suppressor-ContainingStrains of Bacillus subtilis. JAMES N. BAPTIST, M. J. TEVETHIA, MANLEY MANDEL,AND CHARLES R. SHAW.................................................. 976

Genetic Analyses of the Polarity Alleles in Recombinants from Mitochondrial GeneticCrosses. NEIL HOWELL, RuTH M. HALL, ANTHONY W. LINNANE, AND H. B. LUKINS 1063

Complementation Between Different Mutations in the ilvA Gene of Escherichia coliK-12. RENEE FAVRE, MAURIZIO ICCARINO, AND MARK LEVINTHAL.......... 1069

Transformation of Salmonella typhimurium by Plasmid Deoxyribonucleic Acid. EstherM. LEDERBERG AND STANLEY N. COHEN................................. 1072

Physiology and MetabolismLipophilic Proteins of Mitochondria from Microaerobic and Aerobic Continuous Cul-

tures of Saccharomyces cerevisiae. P. J. ROGERS AND P. R. STEWART.......... 653Citrate Metabolism in Aerobacter cloacae. R. W. O'BRIEN AND JARMILA GEISLER.... 661Autolysis in Staphylococcus aureus: Preferential Release of Old Cell Walls. R. W. GIL-

PIN, S. NARROD, W. WONG, F. E. YOUNG, AND A. N. CHATTERJEE .......... 672Sugar Catabolism in Aquaspirillum gracile. BARBARA E. LAUGHON AND NOEL R. KREIG. 691Genetic Evidence for the Physiological Significance of the D-Tagatose 6-Phosphate

Pathway of Lactose and D-Galactose Degradation in Staphylococcus aureus. DON-ALD L. BISSETT AND RICHARD L. ANDERSON............................... 698

Biotin Uptake by Cold-Shocked Cells, Spheroplasts, and Repressed Cells of Saccharo-myces cerevisiae: Lack of Feedback Control. JOHN F. CICMANEC AND HERMAN C.LICHSTEIN............................................................... 718

Culture Medium for Enterobacteria. FREDERICK C. NEIDHARDT, PHILIP L. BLOCH,AND DAVID F. SMITH.................................................... 736

Initiation of Spore Germination in Bacillus subtilis: Relationship to Inhibition Of L-Ala-nine Metabolism. CHANDAN PRASAD....................................... 805

Characterization of Lactose-Fermenting Revertants from Lactose-Negative Strepto-coccus lactis C2 Mutants. B. R. CORDS AND L. L. MCKAY ...... .............. 830

Bacterial Calcium Transport: Energy-Dependent Calcium Uptake by MembraneVesicles from Bacillus megaterium. ELLIS E. GOLUB AND FELIX BRONNER...... 840

Physiological Roles of Pneumococcal Peptidases. MARY K. JOHNSON............... 844Characterization of Salmonella typhimurium Strains Sensitive and Resistant to Methio-

nine Sulfoximine. KATHELYN STEIMER-VEALE AND JEAN E. BRENCHLEY....... 848Hydrocarbon Metabolism by Brevibacterium erythrogenes: Normal and Branched Al-

kanes. M. P. PIRNIK, R. M. ATLAS, AND R. BARTHA....................... 868Cross-Pathway Regulation: Tryptophan-Mediated Control of Histidine and Arginine

Biosynthetic Enzymes in Neurospora crassa. M. CARSIOTIS AND RICHARD F. JONES. 889Cross-Pathway Regulation: Histidine-Mediated Control of Histidine, Tryptophan, and

XV1 CONTENTS

CONTENTS XVll

Arginine Biosynthetic Enzymes in Neurospora crassa. M. CARSIOTIS, RICHARD F.JONES, AND ANNE C. WESSELING.......................................... 893

Photoinactivation of Ammonia Oxidation in Nitrosomonas. ALAN B. HOOPER ANDKATHLEEN R. TERRY.................................................... 899

Surface Polysaccharide from Staphylococcus aureus M That Contains Taurine, D-Amino-galacturonic Acid, and D-Fucosamine. DENG-FONG LIAU, M. ANN MELLY, ANDJOHN H. HASH.......................................................... 913

Bacterial Metabolism of para- and meta-Xylene: Oxidation of a Methyl Substituent.JOHN F. DAVEY AND DAVID T. GIBSON................................... 923

Bacterial Metabolism of para- and meta-Xylene: Oxidation of the Aromatic Ring.DAVID T. GIBSON, VENKATANARAYANA MAHADEVAN, AND JOHN F. DAVEY...... 930

Heterogeneity in Lipid Composition of the Outer Membrane and Cytoplasmic Mem-brane of Pseudomonas. D. L. DIEDRICH AND E. H. CoTA-ROBLES........... 1006

Orthophosphate Requirement for the Formation of Phosphoenolpyruvate from Pyru-vate by Enzyme Preparations from Photosynthetic Bacteria. BOB B. BUCHANAN. 1066

EnzymologyPurification and Properties of (+)-cis-Naphthalene Dihydrodiol Dehydrogenase of

Pseudomonas putida. T. R. PATEL AND D. T. GIBSON....................... 879Purification and Properties of Pyridine Nucleotide-Independent L-Lactate Dehydro-

genase from Polyporus circinatus. Shigehiro Funayama and Glaci T. Zancan... 1000

INDEX TO DATE OF ISSUE

Month Date of Issue Pages

July 12 July 1974 1-338August 13 August 1974 339-652September 19 September 1974 653-1074

INDEX TO ADVERTISERS

APPLiED BIo-SCIENCES........................... 8

* BELLCO GLAss Co.................................................... 9

* DIFco LABORATORIES.......................................... COVER 4

GIBCO DIAGNOSTICS .................. .. ........................... 11

HARLECO.......................................... COVER 2

PHARMACiA FINE CHEMICALS, INC............................7, 12

* Sustaining Member, American Society for Microbiology