Sample to Insight Join the QIAGEN Cancer Research Community Get exclusive access to our first Cancer Insights Sessions Sign up for our first activities and be part of the new QIAGEN Cancer Research Community from the beginning – learn about the value of multi- modality approaches in liquid biopsy analysis and how to achieve a more complete picture of liquid biopsies by analyzing circulating tumor cells, extracellular vesicles and cell-free DNA in parallel. Podcasts and Science Talks • RNA Profiles of Circulating Tumor Cells and Extracellular Vesicles • Cell-Free DNA Variant Sequencing Using CTC-Depleted Blood • Molecular Analysis Research of Circulating Tumor Cells, Extracellular Vesicles and Nucleic Acids in Liquid Biopsies • QIAGEN R&D experts in liquid biopsy analysis discuss findings in a webcast talk show Coming soon: look out for further insights into CTCs and FFPE samples.
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Sample to Insight
Join the QIAGENCancer ResearchCommunityGet exclusive access to our firstCancer Insights Sessions
Sign up for our first activities and be part of the new QIAGEN Cancer
Research Community from the beginning – learn about the value of multi-
modality approaches in liquid biopsy analysis and how to achieve a more
complete picture of liquid biopsies by analyzing circulating tumor cells,
extracellular vesicles and cell-free DNA in parallel.
Podcasts and Science Talks• RNA Profiles of Circulating Tumor Cells and Extracellular Vesicles
• Cell-Free DNA Variant Sequencing Using CTC-Depleted Blood
• Molecular Analysis Research of Circulating Tumor Cells, Extracellular
Vesicles and Nucleic Acids in Liquid Biopsies
• QIAGEN R&D experts in liquid biopsy analysis discuss findings in
a webcast talk show
Coming soon: look out forfurther insights into CTCsand FFPE samples.
Join the QIAGEN Cancer Research Community 02/2020 32 Join the QIAGEN Cancer Research Community 02/2020
Quick-Start Workflow for multianalyte liquid biopsy research
No storage possible
Blood supernatantPool 2 x 5 ml
Product Cat. no.
PAXgene® Blood ccfDNA Tubes 768115*
AdnaTest CTC Select 395092
QIAamp® MinElute® ccfDNA Mini Kit 55204
exoRNeasy Maxi Kit 77164
* Available in United States; not available in all countries. For Research Use Only.Not for use in diagnostic procedures.
† Recommended conditions for PAXgene Blood ccfDNA Tube:1. centrifugation step at 1900 x g, 15 min, at room temperature.2. Optional: second centrifugation step at 1900 x g, 10 min, at room temperature.
‡ 16,000 x g will remove some of the larger extracellular vesicles
Whole blood
2 x 10 ml
or
For ccfDNA analysis: up to 25°C for ≤10 daysup to 30°C for ≤7 daysup to 37°C for ≤3 days
Blood sampling andstabilization of CTCs:
PAXgene® Blood ccfDNA Tube
up to
hours at 2–8°C72
Blood samplingand stabilization:
EDTA tube
up to
hours at 2–8°C4
CTC lysate
Store for up to4 weeks at –20°C
Protocol HB-2366-002
QIAamp MinEluteccfDNA Mini Kit
Centrifugation†
1. 1900 x g, 10 min at 4°C2. 16000 x g, 10 min at 4°C
Optional bloodgDNA control
2 x 5 ml
Residual bloodcells
Analysis
Plasma 4 ml
Store for up to1 month at –20 °C
> 1 month at –80 °C
AdnaTest CTC SelectProtocol HB-2519-002
Step 1 206
• NGS • Digital PCR • qPCR
cfDNA
• NGS • qPCR • Pyrosequencing
CTC nucleic acids
• NGS • qPCR
Exosomal RNA
Protocol HB-2630-002
exoRNeasyMaxi Kits
0.8 µm or16,000 x g, 10 min at 4°C
or filter‡
Centrifugation
10 ml1. 1900 x g, 10 min at 4°C2. 3000 x g, 15 min at 4°C
Plasma 4 ml
Store for up to1 month at –20 °C
> 1 month at –80 °C
Product Cat. no.
PAXgene® Blood ccfDNA Tubes 768115*
AdnaTest CTC Select 395092
QIAamp® MinElute® ccfDNA Mini Kit 55204
exoRNeasy Maxi Kit 77164
No storage possible
Blood supernatantPool 2 x 5 ml
Product Cat. no.
PAXgene® Blood ccfDNA Tubes 768115*
AdnaTest CTC Select 395092
QIAamp® MinElute® ccfDNA Mini Kit 55204
exoRNeasy Maxi Kit 77164
* Available in United States; not available in all countries. For Research Use Only.Not for use in diagnostic procedures.
† Recommended conditions for PAXgene Blood ccfDNA Tube:1. centrifugation step at 1900 x g, 15 min, at room temperature.2. Optional: second centrifugation step at 1900 x g, 10 min, at room temperature.
‡ 16,000 x g will remove some of the larger extracellular vesicles
Whole blood
2 x 10 ml
or
For ccfDNA analysis: up to 25°C for ≤10 daysup to 30°C for ≤7 daysup to 37°C for ≤3 days
Blood sampling andstabilization of CTCs:
PAXgene® Blood ccfDNA Tube
up to
hours at 2–8°C72
Blood samplingand stabilization:
EDTA tube
up to
hours at 2–8°C4
CTC lysate
Store for up to4 weeks at –20°C
Protocol HB-2366-002
QIAamp MinEluteccfDNA Mini Kit
Centrifugation†
1. 1900 x g, 10 min at 4°C2. 16000 x g, 10 min at 4°C
Optional bloodgDNA control
2 x 5 ml
Residual bloodcells
Analysis
Plasma 4 ml
Store for up to1 month at –20 °C
> 1 month at –80 °C
AdnaTest CTC SelectProtocol HB-2519-002
Step 1 206
• NGS • Digital PCR • qPCR
cfDNA
• NGS • qPCR • Pyrosequencing
CTC nucleic acids
• NGS • qPCR
Exosomal RNA
Protocol HB-2630-002
exoRNeasyMaxi Kits
0.8 µm or16,000 x g, 10 min at 4°C
or filter‡
Centrifugation
10 ml1. 1900 x g, 10 min at 4°C2. 3000 x g, 15 min at 4°C
Plasma 4 ml
Store for up to1 month at –20 °C
> 1 month at –80 °C
No storage possible
Blood supernatantPool 2 x 5 ml
Product Cat. no.
PAXgene® Blood ccfDNA Tubes 768115*
AdnaTest CTC Select 395092
QIAamp® MinElute® ccfDNA Mini Kit 55204
exoRNeasy Maxi Kit 77164
* Available in United States; not available in all countries. For Research Use Only.Not for use in diagnostic procedures.
† Recommended conditions for PAXgene Blood ccfDNA Tube:1. centrifugation step at 1900 x g, 15 min, at room temperature.2. Optional: second centrifugation step at 1900 x g, 10 min, at room temperature.
‡ 16,000 x g will remove some of the larger extracellular vesicles
Whole blood
2 x 10 ml
or
For ccfDNA analysis: up to 25°C for ≤10 daysup to 30°C for ≤7 daysup to 37°C for ≤3 days
Blood sampling andstabilization of CTCs:
PAXgene® Blood ccfDNA Tube
up to
hours at 2–8°C72
Blood samplingand stabilization:
EDTA tube
up to
hours at 2–8°C4
CTC lysate
Store for up to4 weeks at –20°C
Protocol HB-2366-002
QIAamp MinEluteccfDNA Mini Kit
Centrifugation†
1. 1900 x g, 10 min at 4°C2. 16000 x g, 10 min at 4°C
Optional bloodgDNA control
2 x 5 ml
Residual bloodcells
Analysis
Plasma 4 ml
Store for up to1 month at –20 °C
> 1 month at –80 °C
AdnaTest CTC SelectProtocol HB-2519-002
Step 1 206
• NGS • Digital PCR • qPCR
cfDNA
• NGS • qPCR • Pyrosequencing
CTC nucleic acids
• NGS • qPCR
Exosomal RNA
Protocol HB-2630-002
exoRNeasyMaxi Kits
0.8 µm or16,000 x g, 10 min at 4°C
or filter‡
Centrifugation
10 ml1. 1900 x g, 10 min at 4°C2. 3000 x g, 15 min at 4°C
Plasma 4 ml
Store for up to1 month at –20 °C
> 1 month at –80 °C
No storage possible
Blood supernatantPool 2 x 5 ml
Product Cat. no.
PAXgene® Blood ccfDNA Tubes 768115*
AdnaTest CTC Select 395092
QIAamp® MinElute® ccfDNA Mini Kit 55204
exoRNeasy Maxi Kit 77164
* Available in United States; not available in all countries. For Research Use Only.Not for use in diagnostic procedures.
† Recommended conditions for PAXgene Blood ccfDNA Tube:1. centrifugation step at 1900 x g, 15 min, at room temperature.2. Optional: second centrifugation step at 1900 x g, 10 min, at room temperature.
‡ 16,000 x g will remove some of the larger extracellular vesicles
Whole blood
2 x 10 ml
or
For ccfDNA analysis: up to 25°C for ≤10 daysup to 30°C for ≤7 daysup to 37°C for ≤3 days
Blood sampling andstabilization of CTCs:
PAXgene Blood ccfDNA Tube
up to
hours at 2–8°C72
Blood samplingand stabilization:
EDTA tube
up to
hours at 2–8°C4
CTC lysate
Store for up to4 weeks at –20°C
Protocol HB-2366-002
QIAamp MinEluteccfDNA Mini Kit
Centrifugation†
1. 1900 x g, 10 min at 4°C2. 16000 x g, 10 min at 4°C
Optional bloodgDNA control
2 x 5 ml
Residual bloodcells
Analysis
Plasma 4 ml
Store for up to1 month at –20 °C
> 1 month at –80 °C
AdnaTest CTC SelectProtocol HB-2519-002
Step 1 206
• NGS • Digital PCR • qPCR
cfDNA
• NGS • qPCR • Pyrosequencing
CTC nucleic acids
• NGS • qPCR
Exosomal RNA
Protocol HB-2630-002
exoRNeasyMaxi Kits
0.8 µm or16,000 x g, 10 min at 4°C
or filter‡
Centrifugation
10 ml1. 1900 x g, 10 min at 4°C2. 3000 x g, 15 min at 4°C
Plasma 4 ml
Store for up to1 month at –20 °C
> 1 month at –80 °C
No storage possible
Blood supernatantPool 2 x 5 ml
Product Cat. no.
PAXgene® Blood ccfDNA Tubes 768115*
AdnaTest CTC Select 395092
QIAamp® MinElute® ccfDNA Mini Kit 55204
exoRNeasy Maxi Kit 77164
* Available in United States; not available in all countries. For Research Use Only.Not for use in diagnostic procedures.
† Recommended conditions for PAXgene Blood ccfDNA Tube:1. centrifugation step at 1900 x g, 15 min, at room temperature.2. Optional: second centrifugation step at 1900 x g, 10 min, at room temperature.
‡ 16,000 x g will remove some of the larger extracellular vesicles
Whole blood
2 x 10 ml
or
For ccfDNA analysis: up to 25°C for ≤10 daysup to 30°C for ≤7 daysup to 37°C for ≤3 days
Blood sampling andstabilization of CTCs:
PAXgene® Blood ccfDNA Tube
up to
hours at 2–8°C72
Blood samplingand stabilization:
EDTA tube
up to
hours at 2–8°C4
CTC lysate
Store for up to4 weeks at –20°C
Protocol HB-2366-002
QIAamp MinEluteccfDNA Mini Kit
Centrifugation†
1. 1900 x g, 10 min at 4°C2. 16000 x g, 10 min at 4°C
Optional bloodgDNA control
2 x 5 ml
Residual bloodcells
Analysis
Plasma 4 ml
Store for up to1 month at –20 °C
> 1 month at –80 °C
AdnaTest CTC SelectProtocol HB-2519-002
Step 1 206
• NGS • Digital PCR • qPCR
cfDNA
• NGS • qPCR • Pyrosequencing
CTC nucleic acids
• NGS • qPCR
Exosomal RNA
Protocol HB-2630-002
exoRNeasyMaxi Kits
0.8 µm or16,000 x g, 10 min at 4°C
or filter‡
Centrifugation
10 ml1. 1900 x g, 10 min at 4°C2. 3000 x g, 15 min at 4°C
Plasma 4 ml
Store for up to1 month at –20 °C
> 1 month at –80 °C
No storage possible
Blood supernatantPool 2 x 5 ml
Product Cat. no.
PAXgene® Blood ccfDNA Tubes 768115*
AdnaTest CTC Select 395092
QIAamp® MinElute® ccfDNA Mini Kit 55204
exoRNeasy Maxi Kit 77164
* Available in United States; not available in all countries. For Research Use Only.Not for use in diagnostic procedures.
† Recommended conditions for PAXgene Blood ccfDNA Tube:1. centrifugation step at 1900 x g, 15 min, at room temperature.2. Optional: second centrifugation step at 1900 x g, 10 min, at room temperature.
‡ 16,000 x g will remove some of the larger extracellular vesicles
Whole blood
2 x 10 ml
or
For ccfDNA analysis: up to 25°C for ≤10 daysup to 30°C for ≤7 daysup to 37°C for ≤3 days
Blood sampling andstabilization of CTCs:
PAXgene® Blood ccfDNA Tube
up to
hours at 2–8°C72
Blood samplingand stabilization:
EDTA tube
up to
hours at 2–8°C4
CTC lysate
Store for up to4 weeks at –20°C
Protocol HB-2366-002
QIAamp MinEluteccfDNA Mini Kit
Centrifugation†
1. 1900 x g, 10 min at 4°C2. 16000 x g, 10 min at 4°C
Optional bloodgDNA control
2 x 5 ml
Residual bloodcells
Analysis
Plasma 4 ml
Store for up to1 month at –20 °C
> 1 month at –80 °C
AdnaTest CTC SelectProtocol HB-2519-002
Step 1 206
• NGS • Digital PCR • qPCR
cfDNA
• NGS • qPCR • Pyrosequencing
CTC nucleic acids
• NGS • qPCR
Exosomal RNA
Protocol HB-2630-002
exoRNeasyMaxi Kits
0.8 µm or16,000 x g, 10 min at 4°C
or filter‡
Centrifugation
10 ml1. 1900 x g, 10 min at 4°C2. 3000 x g, 15 min at 4°C
Plasma 4 ml
Store for up to1 month at –20 °C
> 1 month at –80 °C
* Available in United States; not available in all countries.For Research Use Only. Not for use in diagnostic procedures.
† Recommended conditions for PAXgene Blood ccfDNA Tube:1. Centrifugation step at 1900 x g, 15 min, at room temperature.2. Optional: second centrifugation step at 1900 x g, 10 min,at room temperature.
‡ 16,000 x g will remove some of the larger extracellular vesicles
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