Johnny Yin 18 April, 2014 Expert of yeast origin nutrients for “Microorganisms”

Johnny Yin

18 April, 2014

Johnny Yin

18 April, 2014

Expert of yeast origin nutrients for “Microorganisms”

Contents

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2

3

Introduction about Angel yeast Co.,Ltd.

Angel yeast extracts – Good nutrient-sources for microoganisms

Application of Angel yeast extract

1

1. Introduction of Angel Yeast Co.,Ltd.1.1 About us >1.2 Global net work > 1.3 Our history >1.4 Main business >1.5 Angel’s value chain >1.6 Employees >1.7 Advantages >

1.1 About us

• Angel yeast, founded in 1986, is a listed high-tech yeast company in China.

• It is one of the largest global yeast and yeast extract manufacturers with 12 factories in China and Egypt up to now.

1.2 Global network

12Factories 4Yeast extract plants

12Technical centers 140Countries

1989 2000 20031986

Project started Public company No.1 in Asia

First facilities First yeast extract facilities

Global supplier

Today2008

1.3 History

The fastest growing company in global yeast industry

• Angel yeast is estimated to grow at a CAGR of 20% 2011-2012, while the global yeast market’s CAGR is to be 8.6% (Foodnavigator.com, 2012-04-05).

CAGR 2011-2012

Main business

• Bakery• Yeast extract-savory• Yeast extract-fermentation• Beverage & ethanol• Human health• Enzymes• Animal nutrition

1.4 Main business

1.5 Angel’s Value Chain

MicroorganismMicroorganism

Industrial fermentationIndustrial fermentation

ProductProduct

BrewingBrewing FeedFeed PharmPharmEnvironmentEnvironment EnergyEnergyAgricultureAgricultureFoodFood ChemicalChemical

LabLab

Wine yeastWine yeast

EnzymeYeast extractYeast cell wall

Feed yeast

EnzymeYeast extractYeast cell wall

Feed yeast

EnzymeActive yeastYeast extractInactive yeast

EnzymeActive yeastYeast extractInactive yeast

Yeast extractYeast extract

Alcohol yeastAlcohol yeastYeast extractBio-fertilizer

Yeast extractBio-fertilizer

BakeryEnzyme

SeasoningInactive yeastYeast extract

BakeryEnzyme

SeasoningInactive yeastYeast extract

EnzymeEnzyme

Yeast extractYeast extract

Yeast extractInactive yeast

Technical supportExperiment

Yeast extractInactive yeast

Technical supportExperiment

PackageTrading

PackageTrading

Our global partners

1.6 Employees>• 1331 employees, 70% of which are professional people• Over 100 researchers work together with universities.• 7 international experts employed as senior technical consultants.

Rosenberg (Netherlands) He Chongyue

(Taiwan, China)

　　 Yao Juan Li Xiao Kuang Jinbao Li Pei Li Zhijun

Tilak (U.S.)Jan Hille ((Netherlands)

1.7 Advantages

1.7.1 Strong production output >1.7.2 Professional production process>1.7.3 R&D strength >1.7.4 Quality, safety and consistency >1.7.5 Certification>1.7.6 Environment protection >1.7.7 Global professional service >

1.7.1 Strong production output >

Yichang Plant Yili Plant

Chongzuo Plant Binzhou Plant

Total output of

Yeast is 120.000 tons, Yeast Extract is 46.000 tons.

1.7.2 Professional production process

1.7.3 R&D strength >• 4 sub-headquarters with technical centers

• National yeast technology research and promotion center

• Post-doctoral scientific research workstation

• R & D team led by PhDs and experts

• National recognized laboratory CNAS

• Basic research and Application research laboratory

• Own the first-class test device and pilot plant.

1.7.4 Quality, safety and consistency >

• EU standard design production line

• Stable and consistent quality applies to all production plants

• Distributed control system

• Siemens PCS7 process control system

• Whole-course CIP cleaning

• SAP-ERP Management flat

1.7.5 Certifications >

BRC SEDEX

ISO22000

HALAL KOSHER(OU)

ISO14001ISO9001CNAS

1.7.6 Environment protection >

• First class environment protection facilities in each plant

• The high-concentration organic waste water was made into bio-organic fertilizer.

1.7.7 Global professional service >

• We do not only provide the best quality products, we also provide technical solutions for all our serving industries

• Set up global baking centers• Over 200 application engineers

2. Angel Yeast Extracts – Good nutrient-sources for microoganisms

2.1 What’s yeast extract >2.2 Why choose yeast extract for fermentation industry > 2.3 Range of Angel’s typical products >

2.1 What’s yeast extract?

Yeast extract is a kind of :

• 100% animal free natural ingredient that is made from yeast.

• High performance and stable sources of nutrients for microorganisms

• Clean and safe raw materials for industrial fermentation and culture media

Yeast extract is a kind of :

• 100% animal free natural ingredient that is made from yeast.

• High performance and stable sources of nutrients for microorganisms

• Clean and safe raw materials for industrial fermentation and culture media

Process of yeast extract production

Process of yeast extract production

2.1.1 Molecular weight distribution

2.1.2 Profile of Amino acids

Asp Thr Ser Glu Gly Ala Val Met Ile Leu Tyr Phe Lys His Arg Pro0

1

2

3

4

5

6

7

Con

cent

rati

on (

%)

Amino acids

A0128 A0316 A0418OX3098OX6919

2.1.3 Profile of free Amino acids and Total amino acids

Free Amino acids / Total Amino acids ×100Free Amino acids / Total Amino acids ×100

2.1.4 Trace elements

Name of elements Concentration （ mg/kg）Ca 246Mg 149Zn 86Fe 33Cr 1.26

Mn 0.08K 9172

Na 2022As 0.03Se 0.002Pb 0.19

2.1.5 Vitamins

Name of vitamins Concentration （ mg/kg ）

Vitamin B1 2.7

Vitamin B2 56.9

Niacin 336

Vitamin B6 17.6

Pantothenic acid 29

Free D-Biotin 500 （ μg/100g ）

2.1.6 Nucleic acid and Nucleotides

0.0

0.5

1.0

1.5

2.0

2.5

3.0

Con

cent

rati

on (

%)

RNA

A0128 A0316 A0418 OX3098 OX6919

UMP CMP GMP IMP AMP0.00

0.02

0.04

0.06

0.08

0.10

0.12

Con

cent

rati

on (

mg/

kg)

UMP, CMP, GMP,IMP and AMP

A0128 A0316 A0418 OX3098 OX6919

2.1.7 Functionality of yeast extract

• Increase biomass • Improve the accumulation of metabolites

2.1.8 Stability of yeast extract

Two key indicators: Total nitrogen and Amino nitrogen, For example: Stability of Yeast extract FM902

2.2 Why we choose yeast extract for fermentation industry? • Clean and Safe - Clean and safe natural material for culture media and industrial fermentation - Animal free, no risks of illness - GMO free, Non allergen - Kosher and Halal certified.

• Stable quality - Produced with strict quality control system. - Large industry scale production - No quality change in different seasons

• High performance - Advanced production technology

Beside yeast extract, Angel also produce yeast peptone and inactive yeast for fermentation industry

2.3 Range of Angel’s typical productsMicrofiltered yeast extract FM888 Powder Lab, Biopharmaceuticals

Nutrients-enriched yeast extractFM803

FM505

Powder

PowderProbiotics, Starter culture

Yeast peptoneFP101

LP101

Powder

PasteProbiotics, culture media

Standard yeast extract

FM808

FM902

LM800

LD00

Powder

Powder

Paste

Liquid

Industrial fermentation

Autolyzed & Inactive yeastFM801

YP101

Powder

PowderAntibiotics, APIs

Economical yeast extractFM701

LM815

Powder

PasteAgriculture and environment

Products

FM888 FP101

Which are the most popular products in lab research?

Which are the most popular products in lab research?

Angel Microfiltered yeast extract FM888

• Features and Advantages - High solubility- Easily assimilated by microorganisms and cells- Rich in peptides and growth factors- Low impurities

• Application- Lab research Dehydrated culture media, Cell culture, Diagnostic media- High value-added industrial fermentation Insulins, Hormones, Hyaluronic acids

Angel yeast peptone FP101

• Features and Advantages - Good replacement of Animal origin peptone- Could be combined with yeast extract- Rich in peptides

• Application- Lab research Microorganisms culture, small scale fermentation- Industrial fermentation Probiotics, Enzymes, Hormones, Organic acids

Products

FM808

FM902

LD00

LM800

Which are the most popular products in industrial

fermentation?

Which are the most popular products in industrial

fermentation?

Angel standard yeast extract FM808

• Features and Advantages - Good solubility- High nitrogen level- Rich in peptides- Light color

• Application- Lab research Dehydrated culture media- Industrial fermentation Insulins, Hormones, Hyaluronic acids, Probiotics

Angel standard yeast extract FM902

• Features and Advantages - Total soluble in water- High nitrogen level- Cost effective- High consistency

• Application- Industrial fermentation

For >60 types of fermentation-based products

Angel standard yeast extract FM902

Angel standard yeast extract FM902

Angel standard yeast extract FM902

Angel standard yeast extract FM902

FM902FM902

EnvironmentEnvironment

AgricultureAgriculture

Industrial fermentationIndustrial fermentation

Amino acidEnzymeHormoneOrganic acidProbioticNucleotides / NucleosidesPoly saccharideBiological preservativesAntibiotic

Amino acidEnzymeHormoneOrganic acidProbioticNucleotides / NucleosidesPoly saccharideBiological preservativesAntibiotic

MashroomRhizobiaBiological baitEffective MicroorganismsBio-fertilizer

MashroomRhizobiaBiological baitEffective MicroorganismsBio-fertilizer

Waste water treatment

Environmental microorganisms

Waste water treatment

Environmental microorganisms

Angel standard yeast extract LM800 and LD00

• Features and Advantages - Paste and Liquid form of yeast extract FM902- Rich in peptides- Cost effective- High consistency

• Application- Industrial fermentation

For >60 types of fermentation-based products

Better yeast extract perform better

solubilityDepends

Good solubility means readily assimilated nutrients,

but it depends on specific needs.

Better yeast extract perform lighter

colorDepends

Some fermentation need light color

Better yeast extract perform higher

amino nitrogenDepends

Higher amino nitrogen product is suitable for seed

culture and probiotics, not all the fermentation

Yeast extract should contain low level

active microbesTrue

High level active microbes cause contamination

Less biomass and productivity means

poor quality of yeast extractDepends

40% of this situation means unsuitable process,

condition or culture media formula

Baker’s yeast extract is better than

Brewer’s yeast extractTrue

Brewer’s yeast extract is not stable which come from

spent brewer’s yeast

Baker’s yeast extract must get

Kosher/Halal certificatesTrue

Clean and safe

Yeast extract contain rich protein Depends It depends on the customers specific needs

How to judge the quality?

3. Application of Angel Yeast Extract

3.1 Summary of Angel yeast extract application >3.2 Some experiments of Angel yeast extract >

3.1 Summary of Angel yeast extract application

Biomass products industry - Starter cultures and probiotics for human food products - Cultivation of mashroom and macrofungi Recommendation: FM808, FM803, FM505, FP101

Culture media industry and lab reagent - Bench scale research - Dehydrated culture media, Diagnostics, Cell cuture Recommendation: FM888, FM808

Bioactive molecules and chemicals industry - Amino acids and peptides - Organic acids, Vitamins, Nucleotides, Enzymes, Alcohol - Vitamins, Antibiotics, Hormones, Biological preservatives - Other types of large scale fermentation Recommendation: FM808, FM902, LM800, LD00, YP101

Agricultural and environmental industry - Sewage treatment, Bio-pesticide, Bio-plastic - Probiotics for agriculture and environment Recommendation: FM902, FM801, FM701, LM815, LP101

3.2 Some experiments of Angel yeast extract

3.2.1 Morphology of 4 types of typical microorganisms >3.2.2 Some experiments of Angel yeast extract >

3.2.1 Morphology of 4 types of typical microorganisms after using yeast extract and other type of organic nitrogen

A B

AA

A

BB

B

“A”—Another type of organic nitrogen “B”—Angel yeast extract

Streptomyces lincolnensis Lactobacillus bulgaricus

Bacillus subtilis Acetobacterium

3.2.2 Angel standard yeast extract FM808 for Lactobacillus thermophilus growth

0

20

40

60

80

100

12 16 20 24 28 32 36 40 44 48

安琪酵母浸粉 FM888

国外某试剂级酵母浸粉

0

2

4

6

8

10

12

14

16

18

12 16 20 24 28 32 36 40 44 48

安琪酵母浸粉 FM888

国外某试剂级酵母浸粉

Strain: Lactobasillus thermophilus

Medium: Yeast Extract FM808 0.3% ， Glucose 2%, etc. pH: 6.5

Result: Yeast extract FM808 could improve the growth of Lactobacillus thermophilus well

Angel yeast extract FM808Angel yeast extract FM808

ControlControl

Angel yeast extract FM808Angel yeast extract FM808

ControlControl

3.2.3 Angel standard yeast extract FM902 for the growth of E.coli and Absidia coerulea

The growth curve of E.coli, comparison of FM902 and Brewer’s yeast extract

The conversion rate of HC(from RSA), Absidia coerulea, yeast extract FM902

3.2.4 Angel nutrients enriched yeast extract FM803 for probiotics

0

0.4

0.8

1.2

1.6

2

0 3 6 9 12 15 18 21 24 27 30

时间(h)

OD

600

FM803

国外某试剂级酵母浸粉

0

0.2

0.4

0.6

0.8

1

1.2

0 3 6 9 12 15 18 21 24 27 30时间(h)

OD

600

FM803

国外某试剂级酵母浸粉

Strain: Lactobacillus rhamnosusMedium: MRS

Strain: Lactobacillus helveticusMedium: MRS

Lactobacillus needs complicated nutritional condition for its growth and acid production. Yeast extract is rich in amino acids, small peptides and growth factors which Lactobacillus needs. Yeast extract FM803 is a kind of free amino acids enriched product and could improve the growth of Lactobacillus.

Angel yeast extract FM803Angel yeast extract FM803

ControlControl

Angel yeast extract FM803Angel yeast extract FM803

ControlControl

Fig.1 The effect on L-Tryptophan when different ratio of yeast extracts were used

Fermentation culture medium(%): yeast extract FM902, glucose 2 %, ammonium sulfate 0.4%, potassium dihydrogen phosphate 0.15%, etc.

Conclusion:FM902 is rich in small peptides and free amino acids, and it also contains appropriate polypeptides, vitamins and trace elements. Thus FM902 is suitable for the fermentation of L-Tryptophan, 2% is a proper level of yeast extract ratio.

3.2.5 Angel standard yeast extract FM902For L-Tryptophan

Department of Microbial Nutrition Research

• The research and development of new products

• The optimization of culture media formula

• The suggestion of fermentation process

• Development of tailor-made products

Technical support

Test and scale up platform

HPLC Atomic Absorption Spectrometer

GC Amino acid analyzer

GC-MS Powder Instrument Drawing Instrument Ultrasonic Processors

Pilot Plant Platform for fermentation control The membrane separation system Spray drying system

Any question, please feel free to contact me:

Johnny Yin

Angel Yeast Co.,Ltd.

Address: 168 Cheng Dong Avenue, Yichang, Hubei, China. 443003.

Email: [email protected];

[email protected]

Contact information

THANK YOU!