JB Accepts, published online ahead of print on 15 July …jb.asm.org/content/early/2011/07/15/JB.05485-11.full.pdf · 145 J1interacting protein is the DE AD-box RNA helicase, CshA.
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Characterization of components of the Staphylococcus aureus messenger RNA 1
degradosome holoenzyme-like complex 2
3
4
Christelle M. Roux1, Jonathon P. DeMuth2 and Paul M. Dunman1,2,† 5
6 1Department of Microbiology and Immunology, University of Rochester Medical Center, 7
Rochester, NY, 14642.; 2Department of Microbiology and Pathology, University of Nebraska 8
Medical Center, Omaha, NE, 68198. 9 10 11 12 †To whom correspondence should be addressed: 13
Paul M. Dunman 14
University of Rochester School of Medicine and Dentistry 15
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Figure 1. B. subtilis and S. aureus mRNA degradosome-like complexes. Panel A. B. subtilis RNAdegradosome-like complex (5). Panel B. Deduced S. aureus RNA degradosome-like complex; blue
t h d i t ti b t S d B btili t i A thi k
Bacillus subtilis Staphylococcus aureus
arrows represent shared interactions between S. aureus and B. subtilis proteins. Arrow thicknessindicates the strength of interactions as determined by quantitative β-galactosidase activity results(Supplemental Table 2.). on July 28, 2018 by guest
Figure 3. Bacterial two-hybrid analysis of selected B. subtilis proteins. Panel A.Qualitative analysis of β-galactosidase activity of indicated protein-pairs. Panel B.Corresponding quantitative analysis of β-galactosidase activity; * P ≤ 0.01 (SupplementalTable 4). B. subtilis proteins tested are RNase J1 (J1), RNase J2 (J2), CshA and RnpA.) p ( ), ( ), p
Figure 4. In vitro interaction of purified S. aureus RNase J1 andRNase J2. Panel A. Gel filtration analysis of RNase J1 (dotted line), RNaseJ2 (dashed line), and RNase J1 + RNase J2 (solid line) as measured by
J1 + J2
( ), ( ) yO.D.595nm. Molecular weight size standards are shown above thechromatogram. Panel B. Silver stained 10% SDS-PAGE of eluted RNaseJ1, RNase J2, and RNase J1 + RNase J2; elution fractions are indicated.