Funded by the European Union - GA 737089 1 ChipScope, a new microscope on a chip with superresolution capabilities J. Daniel Prades [email protected]University of Barcelona Department of Electronic and Biomedical Engineering FET-OPEN “Overcoming the Limits of Diffraction with Superresolution Lighting on a Chip”, 2017-2020. Workshop: “European Initiatives for the Next Generation of Nanoscopies in Biosciences”
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J. Daniel Prades - ChipScope€¦ · Funded by the European Union - GA 737089 1 ChipScope, a new microscope on a chip with superresolution capabilities J. Daniel Prades [email protected]
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E. Beitzig, S. W. Hell, and W. E. Moerner, “How the optical microscope became a nanoscope,” in The Nobel Prize in Chemistry, The Nobel lectures (Stockholm University, 2014).
(a) Isolation of vital tissue from patient lung(b) Preparing small tissue samples for outgrowth8c) Brightfield Images from isolated cells in 2D culture (mag. 20x, fibroblasts marked by red circles)
Demonstration application
(c )
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Biocompatibility studies
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Phototoxicity
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Biocompatibility studies
Biocompatible materials
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CMOS Avalanche PhotodiodesElectronics
NanoLED arrays
Image proc. and reconstruction
Microsystems for life sciences
Tissues for intra-cell structure obs.
Dissemination and Training
Multiphysics simulations
Ludwig-Maximilians-Universität München
Universitat de Barcelona
Technische UniversitätBraunschweig
Austrian Instituteof Technology
University of Rome
Expert Ymaging SL
MedizinischeUniversität Wien
Swiss FoundationFor Research in Microtechnology
DNA nanorulers
Consortium
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1. To proof-the-concept of ChipScope by demonstrating microscopy of living tissues with:
• direct imaging with superresolution
• molecular fluorescent imaging with superresolution
• real-time operation (>10 frames per second)
2. To establish the technological basis as well as theoretical understanding of the ChipScope concept: