Shop! BioXtra ™ 2016 Over 100 essential offers and exciting new products Ask your Life Science specialist for your promotional brochure or check out the BioXtra promotions on our website. Valid until 31 st December, 2016 Issue 35 September 2016 www.vwr.com PROMOTIONS FOR LIFE SCIENTISTS – Valid until 31st January, 2017 Valid until 31st January, 2017 Please visit www.vwr.com for full technical specifications and product selection Varioskan ™ LUX Multimode Reader VividPro ™ and VividPro Plus ™ fluorescent total protein stains Slide-A-Lyzer ™ Dialysis Cassettes Western blotting consumables A new source for Protein chromatography resins VWR Chemi only Cat. No. 735-0296P Cat. No. 1B1772-200ULP Cat. No. 514-0263P Cat. No. 786-952P Cat. No. 730-1471P Cat No. 28-9068-36P2 ™ 24.650,00 € read more on page 9 104,00 € read more on page 10 33,25 € read more on page 10 1.060,00 € read more on page 11 9.188,50 € read more on page 12 205,94 € read more on page 13 NEW c Introduction offer! Big savings! 30% OFF Buy 4 and pay for only 3 Buy 3 and pay for 2! Buy 3 and get 1 ECL Start kit for FREE!
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Shop!
BioXtra™ 2016Over 100 essential offers and exciting new products
Ask your Life Science specialist for your promotional brochure or
check out the BioXtra promotions on our website.
Valid until 31st December, 2016
Issue 35 September 2016
www.vwr.com
PROMOTIONS FOR LIFE SCIENTISTS – Valid until 31st January, 2017
Valid until 31st January, 2017 Please visit www.vwr.com for full technical specifications and product selection
Varioskan™ LUX Multimode Reader
VividPro™ and VividPro Plus™
fluorescent total protein stainsSlide-A-Lyzer™ Dialysis Cassettes
Western blotting consumablesA new source for Protein chromatography resins
VWR VWRbioMarke Shop September 2016 Valid until 31st January, 2017
2 Offers are valid until 31st January, 2017Promotions in the VWRbioMarke Shop cannot be used in conjunction with any other pricing agreements from VWR. VWR reserves the right to refuse invalid orders.
Place your order today! Visit our website www.vwr.com
Local price agreements apply.
Omega Bio-tek Total RNA IsolationHP Total RNA Kit
This kit provides a rapid and easy method for RNA isolation from cultured cells or tissues. The kit has been updated to include a unique RNA Homogenizer Mini Column, which doubles to simultaneously remove gDNA and homogenize the sample.
• Reduces processing times by consolidating 2 steps.
• Eliminates column clogging by removing gDNA and cellular debris.
To evaluate the HP Total RNA Kit, order the 5 prep kit free of charge
GENOMICS
Quantabio SYBR and cDNA DealSYBR Deal
Sensitive and precise DNA amplification with DNA-intercalating dye based detection chemistry.
cDNA Deal
qScript cDNA synthesis reagents provide highly sensitive first-strand cDNA synthesis in a variety of easy-to-use reagent configurations for RT-PCR and RT-qPCR.
VWR VWRbioMarke Shop September 2016 Valid until 31st January, 2017
3Place your order today! Visit our website www.vwr.com
Local price agreements apply.
What can be done in seconds?HOMOGENIZE 24 SAMPLES WHILE MAKING YOUR COFFEE!
Get optimal DNA, RNA & protein extraction from the most difficult, large or tiny samples, in less than 30 seconds.
Precellys® Evolution is the most advanced homogenizer gathering high efficiency and versatility for all sample preparation needs. With more than 30 different kits to process animal, plant or microorganisms and its high speed, any homogenization steps will be done in seconds.
• Efficient on every samples: thanks to the high speed of 10 000 rpm
• Easy handling: thanks to the patented automatic tube locking system
• Versatile: Process up to 24x2 ml (or 0.5 ml), 12x7 ml or 6x15 ml tubes
• Protective: compatible with the cooling option Cryolys® to protect sensitive samples
GENOMICS
Description Pack Cat. No. Price, € Promo price, €
Precellys® Evolution 1 unit 432-0304P 8.595,00 8.165,25
7 ml holder pack for Precellys® Evolution 1 unit 432-0307P 981,00 490,50
15 ml holder pack for Precellys® Evolution 1 unit 432-0308P 981,00 490,50
Hard tissue grinding MK28 1 kit 432-3753P 190,00 161,50
Hard tissue homogenizing CK28 1 kit 432-3752P 190,00 161,50
VWR VWRbioMarke Shop September 2016 Valid until 31st January, 2017
4 Offers are valid until 31st January, 2017Promotions in the VWRbioMarke Shop cannot be used in conjunction with any other pricing agreements from VWR. VWR reserves the right to refuse invalid orders.
Place your order today! Visit our website www.vwr.com
Local price agreements apply.
GENOMICS
VWR PCR WorkstationProvides a clean environment for PCR set-up and other sensitive protocols
• Active decontamination of work surface
• Additional inactivation of aerosol-bound contaminants
• Large working area of 72 x 54 cm in stainless steel
• 4 internal power outlets for operating lab equipment
Buy Now and Save 25% on the NEW Automation-compatible Axygen® PCR Microplate!Features
• 96-well half skirt microplates
• Compatible with major thermal cyclers and sequencers
• Raised rims provide superior sealing
• Reinforced frame enhances rigidity
• Ultra-thin walls provide even and consistent heat transfer
• Black alpha-numerical lettering, visible grid lines, and transparent wells facilitate sample traceability
• Nonpyrogenic
• RNase-/DNase-free
• PCR inhibition-free
Split in frame relieves stress during thermal cycling.
Raised rims provide superior sealing.
Defined grid lines enable easy sample tracking.
Clear and ultra-thin walls provide even heat transfer.
Grid improves support and rigidity.
Description Volume Pack Cat No Price, € Promo price, €
VWR VWRbioMarke Shop September 2016 Valid until 31st January, 2017
5Place your order today! Visit our website www.vwr.com
Local price agreements apply.
GENOMICS
VWR® PCR EnzymesVWR Taq DNA Polymerase
A high performance thermostable Taq DNA Polymerase. Highly stable and well suited for routine PCR applications requiring high yield and robust performance.
VWR TEMPase Hot Start DNA Polymerase
For reaction set-up at room temperature. Decreased formation of non-specific priming results in increased sensitivity, specificity and yield Taq DNA Polymerase and TEMPase Hot Start DNA Polymerase are supplied Key Buffer, Extra Buffer and MgCl2
VWR Master Mixes
Convenient alternatives to Taq and TEMPase DNA Polymerases, containing polymerase, buffer, dNTPs and MgCl2. Fewer handling steps reduce set-up time and increase reproducibility. RED Taq Master Mix for direct gel loading 50 µl final reaction volume
VWR VWRbioMarke Shop September 2016 Valid until 31st January, 2017
6 Offers are valid until 31st January, 2017Promotions in the VWRbioMarke Shop cannot be used in conjunction with any other pricing agreements from VWR. VWR reserves the right to refuse invalid orders.
Place your order today! Visit our website www.vwr.com
Local price agreements apply.
GENOMICS
5PRIME HotMaster Taq DNA Polymerase5PRIME HotMaster Taq DNA Polymerase uses an innovative thermostable Taq inhibitor that releases fully active at high temperatures ensuring precise primer annealing and extension at optimal temperatures and “cold-stop” after thermal cycling. Ideal for fast thermal cycling protocols.
• Minimal optimization with self-adjusting magnesium buffer technology
• Novel HotMaster hot-start/cold-stop technology releases full activity at higher temperatures without activation step
• Convenient reaction set-up at ambient temperature
Perfect cycling with VWR PCR CyclersBuy a UNO96 gradient cycler and get a personal Ristretto for FREE
FastDigest Value Pack* 13 x 20 reactions K1991P 43,69 32,77
*Value pack contains 13 of most commonly used FastDigest enzymes: EcoR I, Bgl II, Nde I, Xba I, Pst I, Not I, Hind III, Eco32I, Sma I, BamH I, Xho I, Kpn I, Sal I
ThermoFisher Scientific former Fermentas & Pierce ranges now available through VWR!
VWR VWRbioMarke Shop September 2016 Valid until 31st January, 2017
8 Offers are valid until 31st January, 2017Promotions in the VWRbioMarke Shop cannot be used in conjunction with any other pricing agreements from VWR. VWR reserves the right to refuse invalid orders.
Place your order today! Visit our website www.vwr.com
Local price agreements apply.
CELL BIOLOGY
JetPRIME® & INTERFERin® Transfection ReagentsBe part of the growing number of jetPRIME® & INTERFERin® users in life science labs worldwide!
• Highly efficient transfection reagents
• Gentle mode of action for more robust data and excellent cell viability
• Use less DNA & siRNA
• Ready to use, compatible with serum & antibiotics
JetPRIME® is a powerful and versatile DNA and siRNA transfection reagent for day-to-day transfections in adherent cell lines. INTERFERin® provides high silencing efficiency already at 1 nM siRNA, hence avoiding off-target effects, and can be used in a wide variety of adherent and suspension cells.
PCR Mycoplasma Test KitReady-to-use PCR Mix for the detection of Mycoplasma in Cell Culture
With PanReac AppliChem PCR Mycoplasma PCR test, results are obtained within a few hours, since the presence of contaminant mycoplasma can be easily detected simply by verifying the bands of amplified DNA fragments in electrophoresis.
Features of this kit
• Detects all of mycoplasma species found in cell cultures
• For 20 tests
Kit components
- Reaction mix (including Taq Polymerase)
- Buffer solution
- Positive template control
Description Pack Cat. No Price, € Promo Price, €
PCR Mycoplasma Test Kit 20 tests A3744.0020P 371,00 278,25
VWR VWRbioMarke Shop September 2016 Valid until 31st January, 2017
9Place your order today! Visit our website www.vwr.com
Local price agreements apply.
CELL BIOLOGY
Varioskan™ LUX Multimode ReaderDesigned for fast and reliable results even for the most challenging applications including cell based assays
• Five reading technologies: Absorbance, Fluorescence Intensity, Luminescence, AlphaScreen / AlphaLISA, and Time-Resolved Fluorescence for wide range of applications
• Autocalibration, self diagnostics and SMART safety features for reliable performance day to day, run to run
• Modular and upgradable design enables to meet current and future needs
• Integrated gas module allow optimal conditions for cell based assays with atmospheric control of CO2 and O2
Description Cat. No. Price, € Promo price, €
Varioskan™ LUX, top F, Abs, 735-0296P 29.000,00 24.650,00
Varioskan™ LUX, top F,Abs,L 735-0295P 33.300,00 28.305,00
Varioskan™ LUX, top F,Abs,L,T 735-0301P 37.944,00 32.252,40
Varioskan™ LUX, top F,Abs,L,A 735-0304P 39.270,00 33.379,50
Varioskan™ LUX, top F,Abs,L,A,T 735-0307P 43.248,00 36.760,80
VWR VWRbioMarke Shop September 2016 Valid until 31st January, 2017
10 Offers are valid until 31st January, 2017Promotions in the VWRbioMarke Shop cannot be used in conjunction with any other pricing agreements from VWR. VWR reserves the right to refuse invalid orders.
Place your order today! Visit our website www.vwr.com
Local price agreements apply.
PROTEOMICS
VividPro™ and VividPro Plus™
VividPro™ and VividPro Plus™ are fluorescent total protein stains offering sensitivity of detection down to 10 ng per band of protein. Gels treated with both stains are compatible with mass spectrometry and those stained with VividPro Plus™ may also be used for Western blotting.
VividPro™
• Fast fluorescent staining in under 45 minutes
• Sensitivity to 10 ng per band of protein
• Excitation 284, 366 nm / emission 471 nm (in presence of 0.2 mg/ml BSA)
• Compatible with mass spectrometry
VividPro Plus™
• Choice of two fluorescent staining protocols: co-electrophoretic & post-electrophoretic
• Co-electrophoretic staining requires only 15 minute post-electrophoresis washing
• Sensitivity to 10 ng per band of protein
• Excitation 300, 496 nm / emission 506 nm (in presence of 0.2 mg/ml BSA)
• Compatible with mass spectrometry and Western blotting
Thermo Scientific™ Slide-A-Lyzer™ Dialysis CassettesSlide-A-Lyzer Dialysis Cassettes enable simple and effective dialysis without the hassle of conventional tubing:
• Reliable – maintain the highest possible sample integrity and protection
• Fast and consistent – dialysis with maximum sample recovery
• Convenient – self-floating chambers for buoyancy ensure vertical orientation during dialysis; twist-off cap for pipette-friendly, easy sample loading and retrieval
VWR VWRbioMarke Shop September 2016 Valid until 31st January, 2017
11Place your order today! Visit our website www.vwr.com
Local price agreements apply.
PROTEOMICS
A New Source for ResinsGel Filtration Resins, Hydrophobic Interaction Chromatography (HIC) & Ion Exchange Chromatography
For gel filtration or size exclusion chromatography we now offer Agarose 4B, 6B, CL-4B and CL-6B.
For hydrophobic chromatography we offer 6% agarose with Butyl, Phenyl and Octyl groups to separate biomolecules with relative hydrophobicity. Phenyl format is offered as a high and low sub.
For ion exchange chromatography, we offer 6% agarose with carboxymethyl (CM), a weak cation exchanger; with sulphopropyl (SP), a strong cation exchanger; with diethylaminoethyl (DEAE), a weak anion exchanger; and with quaternary ammonium (Q), a strong anion exchange.
Description Type Size (ml) Cat. No. Price, €
G-Sep™ Agarose 4B Gel Filtration 1000 786-952P 1.060,00
G-Sep™ Agarose 6 Fast Flow Gel Filtration 1000 786-954P 1.600,00
G-Sep™ Agarose 6B Gel Filtration 1000 786-955P 1.040,00
G-Sep™ Agarose CL-4B Gel Filtration 1000 786-953P 1.130,00
G-Sep™ Agarose CL-6B Gel Filtration 1000 786-956P 1.150,00
G-Sep™ Butyl Agarose 6 Fast Flow HIC 25 786-957P 198,00
G-Sep™ Butyl Agarose 6 Fast Flow HIC 200 786-958P 959,00
G-Sep™ CM Agarose Fast Flow Ion Exchange 25 786-965P 99,80
G-Sep™ CM Agarose Fast Flow Ion Exchange 500 786-966P 754,00
G-Sep™ DEAE Agarose Fast Flow Ion Exchange 25 786-967P 99,80
G-Sep™ DEAE Agarose Fast Flow Ion Exchange 500 786-968P 754,00
G-Sep™ Octyl Agarose 6 Fast Flow HIC 25 786-963P 212,00
G-Sep™ Octyl Agarose 6 Fast Flow HIC 200 786-964P 959,00
G-Sep™ Phenyl Agarose 6 Fast Flow (High Sub) HIC 25 786-959P 195,00
G-Sep™ Phenyl Agarose 6 Fast Flow (High Sub) HIC 200 786-960P 959,00
G-Sep™ Phenyl Agarose 6 Fast Flow (Low Sub) HIC 25 786-961P 198,00
G-Sep™ Phenyl Agarose 6 Fast Flow (Low Sub) HIC 200 786-962P 959,00
G-Sep™ Q Agarose Fast Flow Ion Exchange 25 786-969P 99,80
G-Sep™ Q Agarose Fast Flow Ion Exchange 300 786-970P 614,00
G-Sep™ SP Agarose Fast Flow Ion Exchange 25 786-971P 99,80
G-Sep™ SP Agarose Fast Flow Ion Exchange 300 786-972P 614,00
DetectionRequest your copy from your local VWR sales office
VWR VWRbioMarke Shop September 2016 Valid until 31st January, 2017
12 Offers are valid until 31st January, 2017Promotions in the VWRbioMarke Shop cannot be used in conjunction with any other pricing agreements from VWR. VWR reserves the right to refuse invalid orders.
Place your order today! Visit our website www.vwr.com
Local price agreements apply.
PROTEOMICS
VWR Chemi onlyChemiluminescence made easy
The new CHEMI only is dedicated to chemiluminescence imaging. This system is built for high performance and automation and features a next generation high quantum efficiency CCD camera for even greater sensitivity. CHEMI only allows automatic capture of a quality image of any Western blot with a single ‘click’.
Western blot imaging has never been as easy as with the VWR CHEMI only. The system automatically selects the right imaging conditions for any blot - regardless of the chemiluminescent reagents being used. All chemiluminescence applications can easily be handled by the CHEMI. Produce superb images even from the faintest of signals.
The compact instrument sits on any laboratory bench taking up very little space.
The VWR CHEMI only is ideal for small blots (up to 11 x 8 cm). The cooled 16 bit camera (ΔT -57 °C) with fixed lens (f/0.95) and data feedback amazes with a great quantum efficiency (73% @ 425 nm) and high resolution (4 MP).
VWR VWRbioMarke Shop September 2016 Valid until 31st January, 2017
14 Offers are valid until 31st January, 2017Promotions in the VWRbioMarke Shop cannot be used in conjunction with any other pricing agreements from VWR. VWR reserves the right to refuse invalid orders.
Place your order today! Visit our website www.vwr.com
Local price agreements apply.
Pall Laboratory Centrifugal Filtration DevicesAdvance your ability to concentrate
• Low-binding polypropylene construction
• Typically yields greater than 90% recovery
• Omega™ modified polyethersulfone membrane provides high flow rates and low protein binding
VWR VWRbioMarke Shop September 2016 Valid until 31st January, 2017
15Place your order today! Visit our website www.vwr.com
Local price agreements apply.
SUPPORTING PRODUCTS
BRAND Ultra Low Retention Tips, BIO-CERT®
More hydrophobic than PTFE
The surfaces of the Ultra Low Retention tips from BRAND are produced through a special physicochemical process. The homogeneous, defect-free surface thus produced has extremely low surface tension – over 50% less than PTFE. This significantly reduces sample loss and provides substantially higher reproducibility when working with critical media.
• Ideal for biological samples that contain detergents such as Triton™ X-100, SDS, Tween etc.
• No additives that can be leached out! No siliconization of the surface!
• High chemical resistance. Ideal for working with solvents.
• The tips can be autoclaved at 121 °C (2 bar) without damaging the material properties.
Tech specs: BIO-CERT® quality: sterile acc. to ISO 11137 and AAMI guidelines, a SAL of 10-6 is obtained. Free from DNA, RNase, endotoxins and ATP.
VWR VWRbioMarke Shop September 2016 Valid until 31st January, 2017
16 Offers are valid until 31st January, 2017Promotions in the VWRbioMarke Shop cannot be used in conjunction with any other pricing agreements from VWR. VWR reserves the right to refuse invalid orders.
Place your order today! Visit our website www.vwr.com
Local price agreements apply.
SUPPORTING PRODUCTS
VWR Thermal Shake Touch & Cooling Thermal Shake TouchIntuitive 10.9 cm colour LCD touch screen with help topics on every screen
• Rapid heating, cooling, and high speed shaking
• Internal memory stores five separate 5-step programs
Accessory Blocks
Block for 24 x 1,5 ml microtubes (includes a removable rack and cover) (460-0210)
Accessory Blocks
Microplate Thermal Block with lid for standard or deep well plates (460-0208)
3 NEW blocks available; 384-well PCR Thermal Block, 0,2 ml PCR Tube/Strip/Plate
Thermal Block, and 5 ml Eppendorf Tube Block which can be ordered separately:
Description Pack Cat. No. Price, €
VWR Cooling Thermal Shake Touch + free block for 1,5 ml Microtubes 1 460-0196P 2.350,00
VWR VWRbioMarke Shop September 2016 Valid until 31st January, 2017
18 Offers are valid until 31st January, 2017Promotions in the VWRbioMarke Shop cannot be used in conjunction with any other pricing agreements from VWR. VWR reserves the right to refuse invalid orders.
Place your order today! Visit our website www.vwr.com
Local price agreements apply.
Streamline Your StorageHave you outgrown your current cryo storage platform? Do you need a more secure sample tracking system? Upgrade your storage system with Thermo Scientific™ Nunc Universal sample storage systems.
Speed Sample Processing, Ensure Efficiency, and Sample Integrity:
• Ensure best tube quality with Thermo Scientific™ Nunc™ Universal Tubes, the molded-In Cap Gasket makes it easy to close without over-tightening by hand or with tools and maintains sample security at a wide range of temperatures.
• The Thermo Scientific™ VisionMate™ High Speed Barcode Reader instantly decodes tube 2D barcodes and shares the data seamlessly with your LIMS or database.
• Rapidly reads racks of tubes in 24, 48, 96 and 384 formats without any user programming. Now with a standard linear barcode reader that attaches magnetically to the reader housing, the unit can simultaneously track racks of tubes by reading a linear barcode placed or etched on the rack
• Improve storage efficiency with the Thermo Scientific 8-Channel Screw Cap Decapper. Cap/decap a full column screw cap tubes in just four seconds. Access all eight tubes with a single or multichannel pipette, then recap. The motor does all the work.
VWR VWRbioMarke Shop September 2016 Valid until 31st January, 2017
19Place your order today! Visit our website www.vwr.com
Local price agreements apply.
VWR® Mini shake liteThe combination of heating/cooling with shaking makes the Mini shake lite microtube shaking incubator ideal for many life science research applications in molecular biology, biochemistry, and clinical chemistry. Its compact footprint incorporates an intuitive control panel with large multicolor display, allowing users to easily program and view temperature, time and speed settings.
• Eight interchangeable aluminum blocks accommodate from PCR plates to tubes ranging from 0,2 ml – 15 ml
• Accommodates up to 40 x 1,5/2 ml tubes
• Fine-tune speed control
• Rapid heating and cooling, temperature range from 14 ºC below ambient to 100 ºC
• Compact footprint (330x166 mm)
• Speed range from 300 - 1500 rpm
The new brochure covering the whole VWR value range for PCR is available
Request your copy from local sales offices or visit vwr.com for online download or flip catalogue
VWR® FOR PCR
Description Cat. No. Price, €
Mini Shake lite + FOC 40 x 1,5 ml tube block 460-0249P1 1.360,00
Mini Shake lite + FOC 54 x 0,5 ml tube block 460-0249P2 1.360,00
Mini Shake lite + FOC 96 x 0,2ml tube block (for PCR tubes or plates) 460-0249P3 1.360,00
Mini Shake lite + FOC 24 x 15 ml tube block 460-0249P4 1.360,00
Mini Shake lite + FOC ‘Water bath’ block 460-0249P5 1.360,00
Mini Shake lite + FOC 26 x 0,5 ml / 24 x 1,5 ml tube block 460-0249P6 1.360,00
Mini Shake lite + FOC 40 x 0,2 ml block 460-0249P7 1.360,00
Mini Shake lite + FOC 96-well microtiter plate block 460-0249P8 1.360,00
VWR VWRbioMarke Shop September 2016 Valid until 31st January, 2017
EditorVWR International Europe bvbaHaasrode Researchpark Zone 3Geldenaaksebaan 4643001 LeuvenBelgium
CopywritingVWR
Layout and typesettingMarketing Services VWR
PrintingStork, Germany
No part of this publication may be reproduced or copied without prior permission by writing of VWR Europe.
Run94 000 copiesPublication date: September 2016
Due to the high sales volume of promoted articles some items may be temporarily out of stock. Products may vary from picture shown. VWR does not take any responsibility for the content. - VWR Terms and Conditions of Sale apply.
ORDER FROM THE CUSTOMER SERVICE CENTRE OR YOUR VWR PRIMARY DISTRIBUTOR
These offers cannot be used in conjunction with any other pricing agreements from VWR and are exclusively for end users. Prices in €, excl.VAT.