Journal of International Academic Research for Multidisciplinary ISSN 2320 -5083 A Scholarly, Peer Reviewed, Monthly, Open Access, Online Research Journal Impact Factor – 1.393 VOLUME 1 ISSUE 10 NOVEMBER 2013 A GLOBAL SOCIETY FOR MULTIDISCIPLINARY RESEARCH www.jiarm.com A GREEN PUBLISHING HOUSE
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Journal of International Academic Research for Multidisciplinary
ISSN 2320 -5083
A Scholarly, Peer Reviewed, Monthly, Open Access, Online Research Journal
Impact Factor – 1.393
VOLUME 1 ISSUE 10 NOVEMBER 2013
A GLOBAL SOCIETY FOR MULTIDISCIPLINARY RESEARCH
www.jiarm.com
A GREEN PUBLISHING HOUSE
Editorial Board
Dr. Kari Jabbour, Ph.D Curriculum Developer, American College of Technology, Missouri, USA.
Er.Chandramohan, M.S System Specialist - OGP ABB Australia Pvt. Ltd., Australia.
Dr. S.K. Singh Chief Scientist Advanced Materials Technology Department Institute of Minerals & Materials Technology Bhubaneswar, India
Dr. Jake M. Laguador Director, Research and Statistics Center, Lyceum of the Philippines University, Philippines.
Prof. Dr. Sharath Babu, LLM Ph.D Dean. Faculty of Law, Karnatak University Dharwad, Karnataka, India
Dr.S.M Kadri, MBBS, MPH/ICHD, FFP Fellow, Public Health Foundation of India Epidemiologist Division of Epidemiology and Public Health, Kashmir, India
Dr.Bhumika Talwar, BDS Research Officer State Institute of Health & Family Welfare Jaipur, India
Dr. Tej Pratap Mall Ph.D Head, Postgraduate Department of Botany, Kisan P.G. College, Bahraich, India.
Dr. Arup Kanti Konar, Ph.D Associate Professor of Economics Achhruram, Memorial College, SKB University, Jhalda,Purulia, West Bengal. India
Dr. S.Raja Ph.D Research Associate, Madras Research Center of CMFR , Indian Council of Agricultural Research, Chennai, India
Dr. Vijay Pithadia, Ph.D, Director - Sri Aurobindo Institute of Management Rajkot, India.
Er. R. Bhuvanewari Devi M. Tech, MCIHT Highway Engineer, Infrastructure, Ramboll, Abu Dhabi, UAE Sanda Maican, Ph.D. Senior Researcher, Department of Ecology, Taxonomy and Nature Conservation Institute of Biology of the Romanian Academy, Bucharest, Romania Dr. Reynalda B. Garcia Professor, Graduate School & College of Education, Arts and Sciences Lyceum of the Philippines University Philippines Dr.Damarla Bala Venkata Ramana Senior Scientist Central Research Institute for Dryland Agriculture (CRIDA) Hyderabad, A.P, India PROF. Dr.S.V.Kshirsagar, M.B.B.S,M.S Head - Department of Anatomy, Bidar Institute of Medical Sciences, Karnataka, India. Dr Asifa Nazir, M.B.B.S, MD, Assistant Professor, Dept of Microbiology Government Medical College, Srinagar, India. Dr.AmitaPuri, Ph.D Officiating Principal Army Inst. Of Education New Delhi, India Dr. Shobana Nelasco Ph.D Associate Professor, Fellow of Indian Council of Social Science Research (On Deputation}, Department of Economics, Bharathidasan University, Trichirappalli. India M. Suresh Kumar, PHD Assistant Manager, Godrej Security Solution, India. Dr.T.Chandrasekarayya,Ph.D Assistant Professor, Dept Of Population Studies & Social Work, S.V.University, Tirupati, India.
JOURNAL OF INTERNATIONAL ACADEMIC RESEARCH FOR MULTIDISCIPLINARY Impact Factor 1.393, ISSN: 2320-5083, Volume 1, Issue 10, November 2013
50 www.jiarm.com
EXTRACTION, MODELING AND PURIFICATION OF FLAVONOIDS FROM ZEPHYRANTHES CANDIDA
MANOJ KUMAR SINDIRI*
MANASA MACHAVARAPU** DR. MEENA VANGALAPATI***
*M.Tech, Dept. of Chemical Engineering, Center For Biotechnology, AUCE(A), Andhra University, Visakhapatnam, India ** M.Tech, Dept. of Chemical Engineering, Center For Biotechnology, AUCE(A), Andhra University, Visakhapatnam, India
***Associate Professor, Dept. of Chemical Engineering, Center For Biotechnology, AUCE(A), Andhra University, Visakhapatnam, India
ABSTRACT
Zephyranthes candida is a perennial herb has many pharmacological activities
because of flavonoid and other biological components. The objective of present work is to
develop a modelling equation and purification of flavonoids by solvent-solvent extraction and
Column chromatography. Extraction was carried out by 80% methanol as a solvent Soxhlet
extractor. Soxhlet extraction with methanol was carried out with varying time intervals, to
evaluate modelling equation. The proposed modelling equation was E= 7.831 (t) 0.0037. High
yield was obtained for 4hrs of Soxhlet extraction with 80% methanol. Yield of flavonoids
after solvent-solvent extraction and column chromatography was 265.67µg/ml and 270.4
µg/ml respectively. The proposed model showed good agreement with the experimental data.
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