10 International Journal of Recent Research and Review, Vol. XI, Issue 2, June 2018 ISSN 2277 – 8322 Using of 2,5-Dihydroxy Benzyldehyde in Spectrophotometric Assay of Paracetamol in Pharmaceutical Preparations Mohammed. S. Younis, Nabeel .S.Othman Department of Chemistry, College of Science, University of Mosul, Iraq Email : [email protected]Abstract- A simple, accurate and sensitive indirect spectrophotometric method for the determination of paracetamol (PAR) in aqueous solution, the method was based on the oxidative coupling reaction of p-amino phenol (PAP) which resulted from acid hydrolysis of PAR with 2,5-di-hydroxy benzaldehyde (2,5-DHB) in presence of potassium periodate as oxidizing agent to produce colored compound, water-soluble and stable product, which exhibit maximum absorption at 534 nm. Beer’s law was obeyed over the range 0.25-3.0 g.ml -1 of PAR in final volume of 10 ml, with a molar absorptivity of 0.34710 4 l.mol -1 .cm -1 and Sandell’s sensitivity index of 0.04348 g.cm -2 , limit of detection (LOD) 0.0479 g.ml -1 ,limit of quantitation (LOQ) 0.159g.ml -1 , a relative error of 1.33 to 2.14 and relative standard deviation not more than 2.52 % depending on the concentration level. The method has been successfully applied to the determination of PAR in pharmaceutical preparations. Keywords- spectrophotometric, paracetamol, 2,5-dihydroxy benzaldehyde, oxidative coupling. I INTRODUCTION Paracetamol [acetaminophen, N-acetyl-p-aminophenol, 4-acetamidophenol] ,it has analgesic and antipyretic properties with week anti-inflammatory activity, it is used in the symptomatic management of moderate pain and fever [1]. PAR has the following chemical structure [2]. Paracetamol,N-(4-Hydroxyphenyl) acetamide The assay of PAR is still the object of investigation, a survey of the literature showed that various methods have been used for quantitative determination of PAR ,these methods included: HPLC [3-6], voltametric [7-10] and electrochemical [11]. Spectrophotometry is considered as the most convenient analytical technique in pharmaceutical analysis because of its intrinsic, simplicity and availability in most quality control laboratories [12-19]. The objective of the investigated reported in this paper is to evaluated simple and accurate spectrophotometric method for the determination of PAR. The method is based on oxidative coupling of PAP with 2,5-DHB reagent in presence of KIO4 as an oxidizing agent to produced an a soluble and stable colored product. II. EXPIMENTAL SETUP All spectrophotometric measurements were performed on Jasco V-630(Japan) spectrophotometer using 1 cm glass and quartz cells, pH meter type HANNA PH 211 was used for pH reading. Reagents - All chemicals used in this investigation are of analytical – reagent grade, and paracetamol standard material was OH NHC-CH 3 O
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10
International Journal of Recent Research and Review, Vol. XI, Issue 2, June 2018
ISSN 2277 – 8322
Using of 2,5-Dihydroxy Benzyldehyde in Spectrophotometric Assay
of Paracetamol in Pharmaceutical Preparations Mohammed. S. Younis, Nabeel .S.Othman
Department of Chemistry, College of Science, University of Mosul, Iraq