B.Sc: Microbiology and toxicological screening in bivalves Sicilian’s bivalves Farms 6 Months Project
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B.Sc: Microbiology and toxicological screening in bivalves
Sicilian’s bivalves Farms
6 Months Project
Heat Shock
resistant
Salmonella spp.
Escherichia Coli
Paralytic Shellfish PoisoningAmnesic Shellfish PoisoningDiarrheic Shellfish Poisoning
Not well cooked
Well cooked
AIM: Evaluating the presence of either faecal microorganisms and/or algal toxins
Microbiological Screening
1. Samples enrichment
2. Colonies isolation through selective media
3. Biochemical identification:
- Macro-methods- Micro-methods
Bio-toxicological Screening
1. Chemical (pH) and physical treatments (centrifugation) of samples
2. Injection of the sample into blood veins
Sample extraction
Salmonella Escherichia Coli
TBXXLD
TSI
Macro-methodsSelective media
Micro-methodsSelective media
API 20 E: Different dehydrated substrates
Bio-toxicological Screening
Intra-peritoneal injection of the bivalve extract into lab mice
PSP ASP
Results: Luckily none of the samples was found contaminated either from toxic bacteria or algal toxins
Techniques learned : Media and solution preparation Bacteria culturing Microbial screening through biochemistry techniques
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