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• Cripto Loss of Func0on ( Cripto LOF) Guardiola et al., PNAS 2012
• Cripto Gain of Func0on (Cripto GOF)
Modelli Murini condizionali in cellule staminali muscolari
(satelli0)
• OR1-‐Mobilizzazione delle cellule staminali muscolari (satelli0) in modelli murini di danno muscolare acuto
• OR2 -‐ Iden0ficazione di nuovi regolatori delle cellule staminali (Automazione HTS)
IGB “A.B.T.” Gabriella MinchioK
Approcci gene0ci e farmacologici alla rigenerazione muscolare • Proteina Cripto
ricombinante • Delivery sistemico -‐Biopolimeri
Approcci farmacologici
RNA Seq/cellule satelli0
Cripto LOF
96-channel head disposable pipetting tips
The system executes Protocols for targeted differentiation of stem cells Screening of compounds libraries
Advantage/benefits High standard reliability, performance and flexibility Low user intervention Contamination risk reduction
Method standardization Up to 4000 single compuonds simultaneously screened
Complete sterility
Robotic platform: The Cell maker
liquid-handling workstation
laminar air-flow cabinet
medium reservoirs
library MTPs
MTP reader
cell culture MTPs
The CellMaker
+
Cell-based Phenotypic Screening: Identificazione di nuovi regolatori delle
cellule staminali
Cell Proliferation
Cell Colony Phenotype
Targeted Differentiation
• LOPAC • HDAC
Inhibitors
Small molecules
Casalino et al., Journal Molecular Cell Biol, 2011 Casalino et al., Molecular Biotechnology, 2011 Franci et al., under review