Nanosense Prof. dr. ir. Ronald Thoelen 02/12/2014
NanosenseProf. dr. ir. Ronald Thoelen
02/12/2014
The project ‘Nanosense’
NANO - SENSOR
READ-OUT TECHNIQUERECEPTOR
Heat Transfer Resistance (HTM)
MIP
Target-molecule
monomer
MAA
cross-linker
EGDM
CHCl3porogene
initiator
AIBN
+
+
Synthetic receptors (MIPs)
Nanosense
1. Heat-Transfer Resistance Based Analysis of Bioparticles (US2014011198)▪ Applicants: IMEC and UHasselt
▪ Status: granted in EP, close to granting in US
▪ Scope: biosensing device for detection and/or characterization of target DNA and/or RNA bioparticles
▪ Current application not covered by this patent
2. Heat-Transfer Resistance Based Analysis of Bioparticles (US2014242605) ▪ Applicants: IMEC and UHasselt
▪ Status: EP-search report available, office action March 2015
▪ Scope: biosensing device for detection and/or characterization of target bioparticles, a.o. cells or small molecules
▪ Current application covered by this patent application
IP
Literature @ UHasselt
Proof-of-concept
The technique has been proven for:
TARGETs: nicotine, serotonin, histamine, caffeïne, malachite green, biogenic amines, …
ANALYTEs: buffer solutions, water, saliva, urine, blood plasma, wholeblood, …
Dose-response curve of
nicotine in PBS
=> LOD ~ 30 nM
HTM-determination
of serotonin in non-diluted blood plasma
Marloes Peeters et al., Analytical and Bioanalytical Chemistry 405, 6479 – 6487 (2013)
Bram Geerets et al., Sensors 13, 9148 – 9159 (2013).
State-of-the-art
- Use of bulk-MIPs
- Stamping technique / spraycaoting
- Flow-cell and drop-cell
- Home-made setup
Valorisation
• What are the most interesting TARGETs? (market potential, emerging technologies, FTO, etc.)
• Detection limits and dynamic range?
• Complexity of the analytes (preparation necessary?)
• Single testing or multi-parameter testing?
ENVIRONMENTAL FOOD SAFETY HEALTH