Introduction to the Microscope

Introduction to the Microscope

History Types Care Parts

Focusing

Diggs?Hans and Zcharias Janssen?

Who invented the microscope?

Antony van Leeuwenhook

Who really used the microscope?

He figured out that living things are made of cells.

Robert Hooke

Compound-More than one lens-Has own light-Light must see through the sample

Types of Microscopes

Stereomicroscope/Dissecting-Do not need light to pass through object-Low magnification

Types of Microscopes

Electron-Very powerful-Passes electrons through an object rather than light-Coat with gold or platinum to attract particles

Types of Microscopes

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-Support

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-Support

Stage - support

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-support

Stage - support

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-support

Stage - support

Coarse adjust – general focusFine Focus – medium and high power focus

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-Support

Stage - support

Coarse adjust – general focusFine Focus – medium and high power focus

Base - support

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-Support

Stage - support

Coarse adjust – general focusFine Focus – high power focus

Base - support

Light source

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-Support

Stage - support

Coarse adjust – general focusFine Focus – high power focus

Base - support

Light source

Diaphragm – adjusts amount of light

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-Support

Stage - support

Coarse adjust – general focusFine Focus – high power focus

Base - support

Light source

Diaphragm – adjusts amount of light

Stage clips – holds slide

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-Support

Stage - support

Coarse adjust – general focusFine Focus – medium and high power focusBase - support

Light source

Diaphragm – adjusts amount of light

Stage clips – holds slide

Objective Lenses

Label the parts on your microscope picture. Body Tube

Eyepiece/ocular lens magnification = 10X

Arm-Support

Stage - support

Coarse adjust – general focusFine Focus – high power focus

Base - support

Light source

Diaphragm – adjusts amount of light

Stage clips – holds slide

Objective Lenses

Revolving nosepiece

Magnification of a Compound Microscope•Because you are looking through multiple lenses the lenses have a “compounding” effect.•The eyepiece always magnifies 10X

  Eyepiece Objective Lens Magnification

Low Power  

10X 

4X 

40XMedium Power

10X 10X 

100XHigh Power

10X 

40X 400X 

The circular area viewed through the eyepiece.

Magnification – The number of times the image of an object is increased

insize by a lens system

FIELD OF VIEW

The number of times the image of an object is increased in size

by a lens system

MAGNIFICATION

Always carry with 2 hands – one on the base and the other on the arm

Do not force knobs Click Nosepiece to the lowest (shortest) setting

when done – otherwise known as the park position Cover the microscope when done

Place the Slide on the Microscope

Use Stage Clips Click Nosepiece to the

lowest (shortest) setting Look into the Eyepiece Use the Coarse Focus

Follow steps to focus using low power Click the nosepiece to the medium or longest

objective Bring the stage as close as possible to the

objective lens using the Coarse Focusing Knob

Use the Fine Focus Knob to bring the slide into focus