TABLE OF CONTENTS CHAPTER1 CHAPTER2 Introduction to the Biochemistry Laboratory A. Safety in the Laboratory Safety First 2 Material Safety Data Sheets 2 Safe Practices in the Biochemistry Laboratory 2 4 B. Keeping Records and Communicating Experimental Results The Laboratory Notebook 5 Details of the Experimental Write-Up 7 Communicating Results from Biochemistry Research C. Using Biochemical Reagents and Solutions Water Quality 14 Cleaning Laboratory Glassware 15 Solutions: Concentrations and Calculations 14 15 Preparing and Storing Solutions 17 D. Quantitative Transfer of Liquids Pipets and Pipetting 18 Automatic Pipetting Devices 21 18 E. Statistical Analysis of Experimental Data Defining Statistical Analysis 23 The Mean, Sample Deviation, and Standard Deviation Spreadsheet Statistics 28 Statistical Analysis in Practice 23 28 Study Problems Further Reading 30 32 The Computer as a Tool In Biochemistry and Molecular Biology A. Computers in Biochemistry and Molecular Biology Personal Computing in Biochemistry 36 The Computer and the Internet 37 B. The World Wide Web 38 Web Sites for Biochemistry and Molecular Biology 38 5 9 24 35 35 xIII
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Introduction to the Biochemistry Laboratory · Introduction to the Biochemistry Laboratory A. Safety in the Laboratory Safety First 2 Material Safety Data Sheets 2 Safe Practices
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TABLE OF CONTENTS
CHAPTER1
CHAPTER2
Introduction to the Biochemistry Laboratory
A. Safety in the Laboratory
Safety First 2
Material Safety Data Sheets 2
Safe Practices in the Biochemistry Laboratory
2
4
B. Keeping Records and Communicating Experimental Results
The Laboratory Notebook 5
Details of the Experimental Write-Up 7
Communicating Results from Biochemistry Research
C. Using Biochemical Reagents and Solutions
Water Quality 14
Cleaning Laboratory Glassware 15Solutions: Concentrations and Calculations
14
15
Preparing and Storing Solutions 17
D. Quantitative Transfer of LiquidsPipets and Pipetting 18Automatic Pipetting Devices 21
18
E. Statistical Analysis of Experimental Data
Defining Statistical Analysis 23
The Mean, Sample Deviation, and Standard Deviation
Spreadsheet Statistics 28
Statistical Analysis in Practice
23
28
Study Problems
Further Reading
30
32
The Computer as a Tool In Biochemistry and Molecular Biology
A. Computers in Biochemistry and Molecular BiologyPersonal Computing in Biochemistry 36The Computer and the Internet 37
B. The World Wide Web 38
Web Sites for Biochemistry and Molecular Biology 38