Introduction to Introduction to Affymetrix Affymetrix Microarrays Microarrays Why use microarrays? Permits expression profiling of thousands of genes in parallel Why use Affymetrix microarrays? • Well established platform • Arrays for various species • Enables combination of different applications • Support from the bioinformatic community • Well annoted probesets
10
Embed
Introduction to Affymetrix Microarrays Why use microarrays? Permits expression profiling of thousands of genes in parallel Why use Affymetrix microarrays?
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Introduction to Affymetrix Introduction to Affymetrix MicroarraysMicroarrays
Why use microarrays?Permits expression profiling of thousands of genes in parallel
Why use Affymetrix microarrays?• Well established platform• Arrays for various species• Enables combination of different applications • Support from the bioinformatic community• Well annoted probesets
Introduction to microarraysIntroduction to microarraysSteps in analysisSteps in analysis
Scan
Scanner
DesignDesign ExperimentExperiment
DesignDesign ExperimentExperiment
ProbeProbeArrayArray
ProbeProbeArrayArray
PreparePrepareSampleSample
PreparePrepareSampleSample
Wash&
Stain
FluidicsStation
Hybridize
Hybridization Oven
Data Analysis Software
Introduction to microarraysIntroduction to microarraysAffymetrix InstrumentationAffymetrix Instrumentation
5 µm
Thousands of copies of a specificoligonucleotide probe
Image of Hybridized GeneChip Array
>6.5 million differentcomplementary probes
Single stranded, labeled ‘target’
Oligonucleotide ‘probe’
**
**
**
****
*****GeneChip® Array Hybridized Probe Cell
1.28cm
1.28cm
Introduction to microarraysIntroduction to microarraysChip features Chip features