Introducing automated ELISA - ChemWell TM Geoffrey Anderson Unitech Scientific LLC Lakewood, CA Visit us: .

Introducing automated ELISA - ChemWellTM

Geoffrey AndersonGeoffrey Anderson

Unitech Scientific LLCUnitech Scientific LLC

Lakewood, CA Lakewood, CA

Visit us:Visit us:

www.unitechscientific.comwww.unitechscientific.com

ChemWellTM Wine Autoanalyzer

Manufactured by Awareness Technology

Unitech Scientific - Exclusive

Distributor

First analyzer in the world to combine biochemistry First analyzer in the world to combine biochemistry and microplate EIA into one instrument.and microplate EIA into one instrument.

ChemWell uses microwells for all reactions, chemistry and EIA.

Windows 95, 98 or XP

Reagents Samples reactions

2 syringe pumps

Wash headWash head

Optical systemOptical system

DI water

Wash and rinse bottles

A ChemWell Tour

ChemWellChemWell

Designed for labs doing:Designed for labs doing:

100 - 1000 tests per day100 - 1000 tests per dayor or

400 EIA per 8 hours400 EIA per 8 hours

Recommended workloadRecommended workload

ChemWellChemWell Features & Benefits

• Easy to Use

• Continuous Sample Loading

• “Stat” sample priority

• Low Operating Cost

• Intuitive, Open Software

• Proven, Reliable Technology

Calibration Options Cut-off (Semi Qualitative)Cut-off (Semi Qualitative) Multi-point Multi-point

Polynomial fitPolynomial fit Cubic Spline fit . . . and more Cubic Spline fit . . . and more

Store Calibration CurvesStore Calibration Curves

Wine Controls: Chart Levy JenningsWine Controls: Chart Levy Jennings

ChemWell runs ELISA as well as Enzymatic reagentsUnitech offers Histamine ELISA kitsUnitech offers Histamine ELISA kitsBotrytis and Aspergillus ELISA kits Botrytis and Aspergillus ELISA kits are in developmentare in development

So let’s take a quick review of the steps and So let’s take a quick review of the steps and principals of ELISA reagents.principals of ELISA reagents.

Immuno Complex: an Antigen with its matching Antibody

An antigen and its antibody fit together like 3 dimensional puzzle pieces.

Ag

Ab

Diagram of a simple ELISA with a positive sample (sample has Ag in it)

Mix the sample and diluent together in a Mix the sample and diluent together in a microwell that is pre-coated with specific Abmicrowell that is pre-coated with specific Ab

Incubate for x minutes until the Ag in the sample Incubate for x minutes until the Ag in the sample binds to the Ab on the well walls.binds to the Ab on the well walls.

Ab

AbAb

Start with an Add the sample Incubate until Ab-coated well with some Ag in it complexed

Ab

AbAb

Ab-Ag

Ab

Ab-Ag

Ag

Ag

Ab

Conjugate is a specific Ab

bound to an Enzyme.

Ab-Ag

AbAb-Ag

Wash out the Add a Incubate until remaining sample conjugate complexed

Ab-Ag

AbAb-Ag

Ab-Ag-Ab-E

AbAb-Ag-Ab-E

Ab-E

Ab-E

The conjugate has a second antibody to the same antigen, allowing you to makean antigen sandwich (with antibody bread). It also leaves an enzyme hanging off.

Ab-E Ab-E

Washing . . . removes the unbound conjugate, without removing bound antibodies.

Ab-Ag-Ab-E

AbAb-Ag-Ab-E

Wash out the Add a Substrate + Enzyme remaining conjugate substrate produces color

Ab-Ag-Ab-E

AbAb-Ag-Ab-E

Ab-Ag-Ab-E-S

AbAb-Ag-Ab-E-S

S

The more Enzyme available, the more color will develop.Many ELISA’s use a stopping solution to end the color generating step before reading.

S S S

Reliable Results:Enzymatic and ELISA

Precise pipettingPrecise pipetting Washes probe inside and outWashes probe inside and out Liquid sensingLiquid sensing Temperature controlledTemperature controlled

Easy to Maintain

Proven Reliability, over 500 sold Proven Reliability, over 500 sold Self DiagnosisSelf Diagnosis Phone/email support by UnitechPhone/email support by Unitech Modular DesignModular Design

Economical to Use

Dramatically reduces LABOR Dramatically reduces LABOR Laboratory Laboratory AdministrativeAdministrative

Warranty

1-year parts & labor Warranty1-year parts & labor Warranty Extended Warranty available Extended Warranty available

6-Month Preventative Maintenance6-Month Preventative Maintenance Free replacement of warranteed partsFree replacement of warranteed parts Other repairs if requiredOther repairs if required Loaner instrument within 72 hoursLoaner instrument within 72 hours

Easy to own Price start at $27,500Price start at $27,500 Reagent rack cooling optionReagent rack cooling option

Add $2000 Add $2000

ChemWell ChemWell Distributed byDistributed by Unitech Scientific Unitech Scientific