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1
Integrated microRNA network analyses identify a poor-prognosis subtype of gastric
cancer characterized by the miR-200 family
Fengju Song 1, Da Yang 4, Ben Liu 1, Yan Guo 1, Hong Zheng 1, Lian Li 1, Tao Wang 5, Jinpu
Yu 2,6, Yanrui Zhao 1, Ruifang Niu 1, Han Liang 3, Hans Winkler 7, Wei Zhang 4, Xishan Hao 3,
and Kexin Chen 1
Departments of 1Epidemiology and Biostatistics, 2Immunology, and 3Gastric Cancer, Key
Laboratory of Cancer Prevention and Therapy, Tianjin, National Clinical Research Center of
Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, 300060, P. R.
China.
4 Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston,
Texas, 77030, USA.
5 Department of Gastroenterology, Tianjin Medical University General Hospital, Tianjin,
300060, P. R. China.
6 TMUCIH–J&J Joint Laboratory, Tianjin Medical University Cancer Institute and Hospital,
Tianjin, 300060, P. R. China
7 Janssen Research and Development, a Division of Janssen Pharmaceutica, NV, 2340 Beerse,
Belgium.
Note: F. Song, D. Yang, and B. Liu contributed equally to this work.
Running title: microRNA network predicts gastric cancer survival
Requests for reprints: Kexin Chen, M.D., Ph.D., Professor and Chair, Department of
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
References: 1. Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin DM. Estimates of worldwide burden of cancer in
2008: GLOBOCAN 2008. Int J Cancer 2010;127:2893-917. 2. Catalano V, Labianca R, Beretta GD, Gatta G, de Braud F, Van Cutsem E. Gastric cancer. Crit Rev Oncol
Hematol 2009;71:127-64. 3. Wu WK, Lee CW, Cho CH, Fan D, Wu K, Yu J et al.. MicroRNA dysregulation in gastric cancer: a new
player enters the game. Oncogene 2010;29:5761-71. 4. Link A, Kupcinskas J, Wex T, Malfertheiner P. Macro-role of microRNA in gastric cancer. Dig Dis
2012;30:255-67. 5. Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 2004;116:281-97. 6. Song FJ, Chen KX. Single-nucleotide polymorphisms among microRNA: big effects on cancer. Chin J
Cancer 2011;30:381-91. 7. Li X, Chen J, Hu X, Huang Y, Li Z, Zhou L et al.. Comparative mRNA and microRNA expression
profiling of three genitourinary cancers reveals common hallmarks and cancer-specific molecular events. PLoS
One 2011;6:e22570. 8. Zhou L, Chen J, Li Z, Li X, Hu X, Huang Y et al.. Integrated profiling of microRNAs and mRNAs:
microRNAs located on Xq27.3 associate with clear cell renal cell carcinoma. PLoS One 2010;5:e15224. 9. Noushmehr H, Weisenberger DJ, Diefes K, Phillips HS, Pujara K, Berman BP et al.. Identification of a
CpG island methylator phenotype that defines a distinct subgroup of glioma. Cancer Cell 2010;17:510-22. 10. Verhaak RG, Hoadley KA, Purdom E, Wang V, Qi Y, Wilkerson MD et al.. Integrated genomic analysis
identifies clinically relevant subtypes of glioblastoma characterized by abnormalities in PDGFRA, IDH1, EGFR,
and NF1. Cancer Cell 2010;17:98-110. 11. Wilkerson MD, Hayes DN. ConsensusClusterPlus: a class discovery tool with confidence assessments and
item tracking. Bioinformatics 2010;26:1572-3. 12. Liu Y, Hayes DN, Nobel A. Statistical Significance of Clustering for High-Dimension, Low-Sample Size
Data. JOURNAL OF THE AMERICAN STATISTICAL ASSOCIATION 2008;103 :1281-93. 13. Yang D, Sun Y, Hu L, Zheng H, Ji P, Pecot CV et al.. Integrated analyses identify a master microRNA
regulatory network for the mesenchymal subtype in serous ovarian cancer. Cancer Cell 2013;23:186-99. 14. Li X, Zhang Y, Zhang Y, Ding J, Wu K, Fan D. Survival prediction of gastric cancer by a
seven-microRNA signature. Gut 2010;59:579-85. 15. Brenner B, Hoshen MB, Purim O, David MB, Ashkenazi K, Marshak G et al.. MicroRNAs as a potential
prognostic factor in gastric cancer. World J Gastroenterol 2011;17:3976-85. 16. Kogo R, Mimori K, Tanaka F, Komune S, Mori M. Clinical significance of miR-146a in gastric cancer
cases. Clin Cancer Res 2011;17:4277-84. 17. Wu WY, Xue XY, Chen ZJ, Han SL, Huang YP, Zhang LF et al.. Potentially predictive microRNAs of
gastric cancer with metastasis to lymph node. World J Gastroenterol 2011;17:3645-51. 18. Kurashige J, Kamohara H, Watanabe M, Hiyoshi Y, Iwatsuki M, Tanaka Y et al.. MicroRNA-200b
regulates cell proliferation, invasion, and migration by directly targeting ZEB2 in gastric carcinoma. Ann Surg
Oncol 2012;19 Suppl 3:S656-64. 19. Hu X, Macdonald DM, Huettner PC, Feng Z, El NI, Schwarz JK et al.. A miR-200 microRNA cluster as
prognostic marker in advanced ovarian cancer. Gynecol Oncol 2009;114:457-64. 20. Baffa R, Fassan M, Volinia S, O'Hara B, Liu CG, Palazzo JP et al.. MicroRNA expression profiling of
human metastatic cancers identifies cancer gene targets. J Pathol 2009;219:214-21.
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
21. Roybal JD, Zang Y, Ahn YH, Yang Y, Gibbons DL, Baird BN et al.. miR-200 Inhibits lung
adenocarcinoma cell invasion and metastasis by targeting Flt1/VEGFR1. Mol Cancer Res 2011;9:25-35. 22. Smith CM, Watson DI, Leong MP, Mayne GC, Michael MZ, Wijnhoven BP et al.. miR-200 family
expression is downregulated upon neoplastic progression of Barrett's esophagus. World J Gastroenterol
2011;17:1036-44. 23. Leskela S, Leandro-Garcia LJ, Mendiola M, Barriuso J, Inglada-Perez L, Munoz I et al.. The miR-200
family controls beta-tubulin III expression and is associated with paclitaxel-based treatment response and
progression-free survival in ovarian cancer patients. Endocr Relat Cancer 2011;18:85-95. 24. Nam EJ, Yoon H, Kim SW, Kim H, Kim YT, Kim JH et al.. MicroRNA expression profiles in serous
ovarian carcinoma. Clin Cancer Res 2008;14:2690-5. 25. Davalos V, Moutinho C, Villanueva A, Boque R, Silva P, Carneiro F et al.. Dynamic epigenetic regulation
of the microRNA-200 family mediates epithelial and mesenchymal transitions in human tumorigenesis.
Oncogene 2012;31:2062-74. 26. Spaderna S, Brabletz T, Opitz OG. The miR-200 family: central player for gain and loss of the epithelial
phenotype. Gastroenterology 2009;136:1835-7. 27. Birchmeier W, Behrens J. Cadherin expression in carcinomas: role in the formation of cell junctions and
the prevention of invasiveness. Biochim Biophys Acta 1994;1198:11-26. 28. Thiery JP. Epithelial-mesenchymal transitions in development and pathologies. Curr Opin Cell Biol
2003;15:740-6. 29. Ru GQ, Wang HJ, Xu WJ, Zhao ZS. Upregulation of Twist in gastric carcinoma associated with tumor
invasion and poor prognosis. Pathol Oncol Res 2011;17:341-7. 30. Kim MA, Lee HS, Lee HE, Kim JH, Yang HK, Kim WH. Prognostic importance of
epithelial-mesenchymal transition-related protein expression in gastric carcinoma. Histopathology
2009;54:442-51. 31. Castro AC, Rosivatz E, Schott C, Hollweck R, Becker I, Sarbia M et al.. Slug is overexpressed in gastric
carcinomas and may act synergistically with SIP1 and Snail in the down-regulation of E-cadherin. J Pathol
2007;211:507-15. 32. Park SM, Gaur AB, Lengyel E, Peter ME. The miR-200 family determines the epithelial phenotype of
cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2. Genes Dev 2008;22:894-907. 33. Hurteau GJ, Carlson JA, Spivack SD, Brock GJ. Overexpression of the microRNA hsa-miR-200c leads to
reduced expression of transcription factor 8 and increased expression of E-cadherin. Cancer Res
post-transcriptional repression of ZFHX1B. Rna 2007;13:1172-8. 35. Renthal NE, Chen CC, Williams KC, Gerard RD, Prange-Kiel J, Mendelson CR. miR-200 family and
targets, ZEB1 and ZEB2, modulate uterine quiescence and contractility during pregnancy and labor. Proc Natl
Acad Sci U S A 2010;107:20828-33. 36. Panda H, Pelakh L, Chuang TD, Luo X, Bukulmez O, Chegini N. Endometrial miR-200c is altered during
transformation into cancerous states and targets the expression of ZEBs, VEGFA, FLT1, IKKbeta, KLF9, and
FBLN5. Reprod Sci 2012;19:786-96. 37. Sheh A, Ge Z, Parry NM, Muthupalani S, Rager JE, Raczynski AR et al.. 17beta-estradiol and tamoxifen
prevent gastric cancer by modulating leukocyte recruitment and oncogenic pathways in Helicobacter
pylori-infected INS-GAS male mice. Cancer Prev Res (Phila) 2011;4:1426-35. 38. Lindblad M, Ye W, Rubio C, Lagergren J. Estrogen and risk of gastric cancer: a protective effect in a
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
nationwide cohort study of patients with prostate cancer in Sweden. Cancer Epidemiol Biomarkers Prev
2004;13:2203-7. 39. Korpal M, Lee ES, Hu G, Kang Y. The miR-200 family inhibits epithelial-mesenchymal transition and
cancer cell migration by direct targeting of E-cadherin transcriptional repressors ZEB1 and ZEB2. J Biol Chem
2008;283:14910-4. 40. Hur K, Toiyama Y, Takahashi M, Balaguer F, Nagasaka T, Koike J et al.. MicroRNA-200c modulates
epithelial-to-mesenchymal transition (EMT) in human colorectal cancer metastasis. Gut 2013;62:1315-26. 41. Ueda T, Volinia S, Okumura H, Shimizu M, Taccioli C, Rossi S et al.. Relation between microRNA
expression and progression and prognosis of gastric cancer: a microRNA expression analysis. Lancet Oncol
2010;11:136-46. 42. Yang ZX, Lu CY, Yang YL, Dou KF, Tao KS. MicroRNA-125b expression in gastric adenocarcinoma and
its effect on the proliferation of gastric cancer cells. Mol Med Rep 2013;7:229-32. 43. Fassan M, Pizzi M, Realdon S, Balistreri M, Guzzardo V, Zagonel V et al.. The
HER2-miR125a5p/miR125b loop in gastric and esophageal carcinogenesis. Hum Pathol 2013;44:1804-10.
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844
Published OnlineFirst December 18, 2013.Clin Cancer Res Fengju Song, Da Yang, Ben Liu, et al. miR-200 familypoor-prognosis subtype of gastric cancer characterized by the Integrated microRNA network analyses identify a
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Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 18, 2013; DOI: 10.1158/1078-0432.CCR-13-1844