This project is supported by the European Union under the Food, Agriculture and Fisheries and Biotechnology theme of the 7 th Framework Programme for Research and Technological Development under Grant Agreement no. 312139 Integrated biotechnological solutions for combating marine oil spills Prof. Nicolas Kalogerakis, Coordinator School of Environmental Engineering Technical University of Crete 3 rd Conference on "Ocean of Tomorrow" Projects, 11 November 2015 , Brussels
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Integrated biotechnological solutions for combating …ec.europa.eu/research/bioeconomy/pdf/ocean_of_tomorrow/...marine oil spills Prof. Nicolas Kalogerakis, Coordinator School of
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This project is supported by the European Union under the Food, Agriculture and Fisheries and Biotechnology theme of the 7th Framework
Programme for Research and Technological Development under Grant Agreement no. 312139
Integrated biotechnological solutions for combating marine oil spills
Prof. Nicolas Kalogerakis, Coordinator School of Environmental Engineering Technical University of Crete
3rd Conference on "Ocean of Tomorrow" Projects, 11 November 2015 , Brussels
Weathering processes & period of activation The essence of
KILL•SPILL
To turn bioremediation from a weathering process into an attractive technology, several “problems” must be overcome (by human intervention):
Speed up the growth of indigenous hydrocarbon degraders or add (previously grown & lyophilized degraders) - bioaugmentation.
Add missing nutrients (primarily N&P) in oligotrophic environments - biostimulation.
Disperse oil into fine droplets (surfactants or biosurfactants)
8. “Mandatory” Field Testing of Technologies Emergency Response Companies as Kill•Spill partners: EPE (Greece) & MMB (Norway)
Development of biosensors for the in-situ monitoring of hydrocarbons at contaminated sites
KIL
L•SP
ILL
Sen
sors
Construction of plasmids encoding sensor/promoter::gfp pairs that, when introduced into a suitable host, respond to the presence of hydrocarbons by producing Green Fluorescent protein.
Sevilla, E., Yuste, L., and Rojo, F., 2015; Marine hydrocarbonoclastic bacteria as whole-cell biosensors for n-alkanes; Microbial Biotechnology, 8: 693-706
Gas CO2
Biodegradation is associated with an enrichment of 13C in the remaining contaminant pool increasing 13C
Compound Specific Isotope Analysis
First KillSpill FISH kits are now
commercially available …
FISH protocols adapted to easy and standardized VIT®
format allows fast and specific on-site monitoring
Identification and quantification of various hydrocarbon-
degrading bacteria using developed test kits:
o Alcanivorax borkumensis
o Cycloclasticus pugetii
o Marinobacter sp.
o Oleispira antarctica
o Oleiphilus messinensis
o Thalassolituus oleivorans
KIL
L•SP
ILL
FISH
kit
s
Green: Alcanivorax borkumensis, Yellow: Oleispira antarctica; Total magnification 1000 x
Green: Alcanivorax borkumensis, Yellow: Oleispira antarctica; Total magnification 1000 x