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DescriptionThe TaKaRa PCR Thermal Cycler Dice™ is a GRADIENT thermal cycler (only for TP600) which allows temperature variation up to a 20°C slope between the right and left sides of the sample block in each temperature setting step. This allows optimization of reaction conditions to be performed in a single experi-ment. In addition, the use of the variable angle wing panel which combines the control panel and display makes the Dice™ compact and functional. It can be placed anywhere in the lab.
Features • Sample Setting is Easy Through use of a "Slide and Lever" Type Cover Handle.Easy Viewing and Access in Any Bench. • The control panel can be set at a desirable angle, providing:
– Easy Programming due to an excellent graphical user interface. – Simple Operation using the pop up menu and alphanumeric key input method, data entry is similar to a cell-phone.
Easy Implementation of PCR reactions• due to the eight preinstalled protocols (PCR programs).No Heat Interference Between Pieces of Equipment • due to reduced absorp-tion from the front and sides, and back gas exhaustion. Extension Time Function• suitable for long PCRExtension Temperature Function • allows touch down PCR Gradient Function (only for TP600) • enables examination of different anneal-ing temperature
Weight 11.5 kg (System Weight)Power requirement/consumption 100-240V, 50/60Hz of AC/ 490 WHeating cooling system peltier elementRamp rate of Heating Maximum 3.0°C/sec.Ramp rate of Cooling Maximum 2.0°C/ sec.Block temperature control range 4-99.9°CBlock temperature accuracy ±0.5°CBlock temperature uniformity ±0.5°CExaggerated shot Less than 0.5°CHeated lid 110°CTemperature extension1 maximum of ±9.9°CTime extension1 maximum of ±9.59 minutesSample capacity 96 × 0.2 mL Tube or 96 well PCR Plate
DisplayBlue/white dot matrix LCD \(240 × 60 dots)
Max. programs stored 100 programsMax. user name registrations 10 users
GRADIENT function2Range which can be set up: 40–75°C (temperature variance : 6–20°C)
1 Touch down PCR2 GRADIENT is set with the first and 12th rows (right end and left end rows) of a heat block (96 wells). GRADIENT function is available only for TP600.
Application Example1. Gradient PCR Using the TaKaRa PCR Thermal Cycler Dice (TP600) System Amplification of 8-kb fragment was performed either in shuttle PCR or conven
tional PCR using λ DNA template
Reaction Mixture TaKaRa Ex Taq™ (5 units/µL) 0.25 µL 10X Ex Taq™ Buffer 5 µL dNTP Mixture (2.5 mM each) 4 µL Primer 1 (20 µM) 0.5 µL Primer 2 (20 µM) 0.5 µL λ DNA 1 µL Sterilized water 38.75 µLTotal volume 50 µL
PCR Conditions Shuttle PCR98 °C 10 sec.52 to 72 °C 5 min. (Gradient temperature : 20°C)
Conventional PCR98 °C 10 sec.45 to 65 °C 30 sec. 30 cycles(Gradient temperature: 20 °C) 5 min. 72 °C 5 min.
Results •ResultsforShuttlePCR 2 step PCR annealing/extension temperature 52°C 72°CM (gradient) M
•ResultsforConventional PCR
3 step PCR annealing temperature 45°C 65°CM (gradient) M
2. One Step RT-PCR
The performance of the TaKaRa PCR Thermal Cycler Dice™ (Cat.# TP600) was evaluated in One Step RNA PCR by using the TaKaRa One Step RNA PCR Kit (Cat.# RR024A) and the TaKaRa PCR Thermal Cycler SP (Cat.# TP400).RT-PCR was performed in two conditions :– In the presence of AMV RTase XL in the reaction mixture.– Without AMV RTase XL in the reaction mixture.
Reaction Mixture 10X One Step RNA PCR Buffer 5 µL 25 mM MgCl2 10 µL dNTP Mixture (10 mM each) 5 µL RNase inhibitor (40 U/µL) 1 µL AMV RTase XL (5 U/µL) or DEPC treated water 1 µL AMV Optimized Taq (5 U/µL) 1 µL Control F-1 Primer 1 (20 µM) 1 µL Control R-1 Primer 2 (20 µM) 1 µL Positive Control RNA 1 µL RNase Free H2O 24 µLTotal Volume 50 µL
PCR Conditions 50°C 15 min. (RT Reaction) 94°C 2 min. (inactivation of RTase) 94°C 30 sec. 60°C 30 sec. 30 cycles72°C 1.5 min.
Results Lane Sample 1 RTase (-) 2 RTase (+) 3 RTase (-) 4 RTase (+) M 100 bp DNA Ladder
After the reaction was completed, a portion of reaction was visualized using agarose gel electrophoresis. In both cases, using TaKaRa PCR Thermal Cycler Dice™ (Cat.# TP600) and TaKaRa PCR Thermal Cycler SP (Cat.# TP400), the control reaction 462 bp product was detected only in the presence of RTase (+).
Related ProductsTaKaRa Micro PCR Tube (0.2 mL with cap) Cat.# 9047 1000 tubesTaKaRa Micro Strip PCR Tube (8 tubes per chain) Cat.# 9148 120 stripsTaKaRa Micro Strip Caps (8 caps per chain) Cat.# 9149 120 stripsTaKaRa 96 well PCR Hi-Plate (12×8 chains/plate) Cat.# NJ111 50 platesTaKaRa PCR Hi-Caps (8 caps per chain) Cat.# NJ811 120 strip
Higher annealing/extension temperatures result in reduced low-molecular nonspecific amplification and increased amplification of the target amplicon.
DescriptionTakara Bio has developed the Thermal Cycler Dice series and recently added the new Dice mini to the series. The convenient Dice Mini will become a standard among personal-type 24-well block thermal cycler systems.
FeaturesThe Program Link Function• , which runs programs in numerical order if the program names are the same and “# number” is entered at the end of the names, enables a complex array of conditions to be set. Up to 22 User Names can be Registered• and up to 200 programs can be stored. Programming is Easy • due to a graphical user interface. Operation is Simple• by utilizing the pop up menu and alphanumeric key input method, the same that are similar to a cell phone. PCR Reaction can be Started Easily. • Due to the eight types of preinstalled protocols (PCR program) . No Heat Interference Between Pieces of Equipment • due to reduced absorption from the front and sides, and back gas exhaustion. Extension Time Function:• Suitable for long PCR Extension Temperature Tunction: • Allows touch down PCR Space-saving Design for Personal Use:• The Dice mini is narrower in width compared to conventional standard systems, saving space on the benchtop (TP600/TP650: 260 mm in width ; TP100: 210 mm in width).
Limited Use Label License: [P10]
Specifications
Product Name TaKaRa PCR Thermal Cycler Dice Mini
Model No. TP100Temperature accuracy +/- 0.5°C (30.0°C to 99.9°C)
+/- 2.0°C (4.0°C to 15.0°C)Temperature uniformity +/- 0.5°C (30.0°C to 99.9°C)
Temperature setting range 4.0°C to 99.9°C
Time setting range 1 second to 99 minutes, 59 seconds, ∞ (up to 9999)Heating cooling system Peltier element
Ramp rate of heating Maximum 3.0°C/sec.Ramp rate of cooling Maximum 2.0°C/sec.Tubes and plates 0.2 mL × 24 tubes
8 Strip of Tubes (0.2 mL) × 3PCR reaction volume settings 5 µL to 150 µL
Program capacity User-names: max. 22 (excluding “Common” and “TaKaRa”)Number of programs: max. 200
Lid temperature Approx. 110°C for the 20.0°C to 99.9°C rangeApprox. 40°C for the 4.0°C to 19.9°C range
Added functions Slope function, Program link•History function•Diagnosis function•PC communications (RS-232)•
(PC transmission software and R5232c cable are not provided)
Display Blue/white dot matrix LCD (240 × 60 dots)Safety devices Power switch with 5 A over-current breaker
Lid Heater temperature fuse (169°C)Thermal guard for reaction block heater plate (85°C)
Power source 100 to 240V AC, 50/60 Hz, 180VADimensions 210 (W) × 370 (D) × 260 (H) mmWeight 7.5 kg
TaKaRa Micro PCR tube (0.2 mL with caps) Cat.# 9047 1000 tubes TaKaRa PCR Micro Strip 8-Tube Cat.# 9148 120 strips TaKaRa PCR Micro Strip 8-Cap Cat.# 9149 120 strips 0.2 mL Hi-Tube Dome Cap Cat.# NJ200 1000 tubes 0.2 mL Hi-Tube Dome Cap Recovery Cat.# NJ201 1000 tubes 0.2 mL Hi-Tube Flat Cap Cat.# NJ202 1000 tubes 0.2 mL Hi-Tube Flat Cap Recovery Cat.# NJ203 1000 tubes 0.2 mL Hi-8-Tube Cat.# NJ300 125 strips 0.2 mL Hi-8-Dome Cap Cat.# NJ301 125 strips 0.2 mL Hi-8-Flat Cap Cat.# NJ302 125 strips TaKaRa 96 well PCR Hi-Plate (12 × 8 chains/plate) Cat.# NJ111 50 plates TaKaRa PCR Hi-Caps (8 caps per chain) Cat.# NJ811 120 strips
PCR tubes
DescriptionsTaKaRa Micro PCR tube (Cat.# 9047):
Tube with attached cap. •Ideal for those applications that use only a few tubes at a time. •
TaKaRa MicroStrip PCR Tube (Cat.# 9148): Eight tube strips allows multiple PCR reactions and is ideal when using an eight •channel pipettor.Packaged 120 strips per unit.•
TaKaRa Micro Strip Caps (Cat.# 9149): 8 Cap Strips. Domed caps eliminate the need for mineral overlay when used in •thermal cyclers with heated lids.Optimized to fit the TaKaRa MycroStrip PCR Tube.•Packaged 120 strips per unit. •
TaKaRa 96 well PCR Hi-Plate (Cat.# NJ111): Robust design to minimize warp.• Raised rims minimize well-to-well contamination.•Thin wall for maximizing efficient heat transfer.•Packaged 50 plates per unit. •
TaKaRa PCR Hi-Caps (Cat.# NJ811): Eight connected caps in strip.•Flat top for comfortable application.•Optimized to fit TaKaRa 96-well PCR plates.•Packaged 120 strips per unit.•
0.2 mL Hi-Tube Dome Cap (Cat.# NJ200)0.2 mL individual PCR tubes with integral domed caps.•Dome-shaped cap for good contact with the heated thermal cycler lid. •
0.2 mL Hi-Tube Dome Cap Recovery (Cat.# NJ201)0.2 mL individual PCR tubes with integral domed caps. •Dome-shaped cap for good contact with the heated thermal cycler lid.•
0.2 mL Hi-Tube Flat Cap (Cat.# NJ202)0.2mL individual PCR tubes with integral flat caps. •Flat caps for easy closure and sample labelling.•
0.2 mL Hi-Tube Flat Cap Recovery (Cat.# NJ203)Flat caps for easy closure and sample labelling.•
0.2 mL Hi-8-Tube (Cat.# NJ300)Strips of 8 attached tubes for use with 0.2 mL strip caps.•
0.2 mL Hi-8-Dome Cap (Cat.# NJ301) Strips of 8 attached tubes for use with 0.2 mL strip tubes.•
0.2 mL Hi-8 Flat Cap (Cat.# NJ302)Strips of 8 attached caps for use with 0.2 mL strip tubes.•Flat caps for easy closure and sample labelling.•
Cat. # NJ200, NJ201, NJ202, NJ203, NJ300, NJ301 and NJ302 are DNase-free, RNase-free and pyrogen free products.
DescriptionThe Mupid®-One electrophoresis system is CE marked. It has novel features including separated power and drain, support for multipipettes, 7 output voltage settings (18, 25, 35, 50, 70, 100, and 135V) and timer function.This product is manufactured by ADVANCE. Mupid® is a registered trademark of ADVANCE.
Features• MultiChannelPipettes:canbeusedwithmultipipettes. The Mupid®-One
allows simultaneous electrophoresis of 104 samples on a single gel (26 wells 4 rows (96 samples + 8 markers)). • The Mupid®-One is wider than the conventional Mupid® series and can support 13 or 26 lanes, enabling sample loading with multipipettors (9 mm pitch for 13 wells, 4.5 mm pitch for 26 wells).
• VoltageRange: Supports seven conventional voltages (18V, 25V, 35V, 50V, 70V, 100V and 135V). Global Input Voltage Compatibility: • variable input voltage of 100 to 240V supports use anywhere in the world Heat-Resistant Materials: • A liquid gel solution up to 100 °C can be poured into the Gel Tray. Clean up can be performed using boiling water. Lengthy Electrophoresis Possible: • wide range for voltage control which improves buffer stability during the run.
ComponentsOne gel casting stand-HR (1)* Several comb setting grooves are provided in the stand. Supports a maximum of
four combs per gel with easy position changed.One-Gel Tray L-HR (1) Accommodates up to four rows of wells (running length of 2.7 cm), running up to 104 samples (26 x 4) is possible.One-Gel Tray S-HR (2) and center partition (1) Used for making 13 and 26 sample gels.One-Comb-HR (4) Four combs can be used for L tray, whereas one comb can be used for S tray.* Number in brackets indicate number of pieces supplied with the chambers
Mupid®-One Cat.# AD160 1 set
Mupid® One
Mupid Specifications
Electrophoresis Cell Overall dimensions 183 mm (W) × 59 mm (H) × 162 mm (L) Material PPHOX Solution volume Approx. 270–320 mL (includes gel tray and gel) Multi-sample Multi-channel pippette compatible Safety Lid Overall dimensions 197 mm (W) × 38 mm (H) × 1709 mm (L) Safety Interlock system Without the lid, main power cannot be operated. Power Supply Overall dimensions 75 mm (W) × 62 mm (H) × 170 mm (L) Weight 410 g Input Voltage AC100 - 240V, (Internationally compatible) 50/60Hz Output Voltage 135V, 100V, 50V, 25V, 70V, 35V, 18V Constant peak voltage of 140V, and duty-control Timer Timer operation 0–99 min, and continuous mode Temporary shutdown supported Memory Function Automatic memory (the last used V and T) One-Gel Tray Gel tray (small) 130 mm (W) × 16.5 mm (H) × 59.5 mm (L) Gel tray (large) 130 mm (W) × 24 mm (H) × 122 mm (L) Quantity small: 2, large: 1 Material Heat Resistant Material (around 100°C of agarose available) One Comb -HR Multiple number of wells 13–26 Wells (13 wells: 9 mm spacing; 26 wells: 4.5 mm spacing) Quantity 4 combs (support making 13 or 26 wells) Material Heat Resistant Material (around 100°C of agarose available) One Gel Casting Stand -HR (makes 2 small gel trays or 1 large gel tray) Overall dimension 140 mm (W) × 20 mm (H) × 125 mm (L)
Gel Maker Set HR (Mupid® One Part #) Cat. # AD240 1 setOne Gel Tray L HR (Mupid® One Part #) Cat. # AD241 2 piecesOne Gel Tray S HR (Mupid® One Part #) Cat. # AD242 4 piecesOne Gel Maker Stand HR (Mupid® One Part #) Cat. # AD243 1 pieceOne Comb-HR (Mupid® One Part #) Cat. # AD244 2 pieces