Instruction manual Microtome M530 Rotary Microtome semi-automatic Manufacturer MEDITE GmbH and publisher: Wollenweberstraße 12 31303 Burgdorf Germany Read manual closely before putting device into operation • Keep manual accessible with device • Contact customer service in case of questions • Specifications are subject to change without prior notice. Rev.: 2 – 06/2017
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Instruction manual Microtome M530
Rotary Microtome semi-automatic
Manufacturer MEDITE GmbH
and publisher: Wollenweberstraße 12
31303 Burgdorf
Germany
Read manual closely before putting device into
operation • Keep manual accessible with device •
Contact customer service in case of questions •
Specifications are subject to change without prior
notice.
Rev.: 2 – 06/2017
Declaration of Conformity
Type of instrument: Rotary Microtome semi-automatic
Model: M530
Serial No.:
We do hereby certify that the above mentioned product meets the requirements set forth in the CE Directives indicated below including all changes and addendums to date thereto. The above mentioned product has been controlled and meets the following directives and standards:
This manual will help you to handling the device. Please read the manual completely and follow the advice. Acquaint yourself to the control elements and their functionality by studying the figures. This way, an adequate use of the device, is ensured. If you have problems, feel free to contact the competent MEDITE contact person.
1.2. Used Symbols and their meanings
1.3. Hazard Sources
Working with the machine contents crushing hazard from the hand wheel and the specimen clamp.
Crushing hazard: Working on moveable components of the machine should be avoided. These warnings are marked with a yellow triangle and a crushing hand inside.
Warning: This symbol warns you of risks for machine, material or environment. Warnings are marked with a yellow warning triangle.
Working with this machine contents cutting hazards from the knife carrier
and specimen clamp (moving).
Cutting hazard: Working with this machine contents cutting hazards for
fingers and hand. These warning are marked with a yellow triangle and a
cutting hand inside.
Notice: Notices, meaning important information for the user, that are not classified as danger or warning, are marked with an information symbol.
Danger warning: This symbol warns you of risks for the life or health of persons. Danger warnings are marked by a red framed triangle.
When working with the Microtome M530, it is necessary, according to you security policies applicable in any case, to wear protective clothings to avoid direct contact with the biologic samples. The same applies, for example, when cleaning the unit.
1.5. Transport and stocking
Observe the following notes for transport and stocking the Microtome M530.
1.6. Environmental conditions
The permissible ambient conditions for operation of the device are listed below. Operation at other ambient conditions is not permitted.
• Operation is only allowed in enclosed rooms,
• Ambient temperature from +15°C to +40°C (without strong fluctuations),
• Only set it on a vibration-free, the weight of the device corresponding laboratory bench,
• Relative air humidity max 80% non-condensing,
• The unit must not be exposed to direct sunlight,
• Maximum height of 2000 m (according to EN 61010-1:2001),
• Mains voltage fluctuation is within + / - 10 % of rated voltage.
Always use the carrying device for lifting and carrying the Microtome M530 make sure to avoid a correct attitude about material things and especially physical damage when lifting!
The Microtome M530 is supplied in a safety transport box. Transporting them always upright and avoid shocks and extreme temperature fluctuations! For the storage of the device an ambient temperature of +5 ° C to +50 ° C is permissible.
Always wear protective clothing (e.g. lab coat, gloves,) to prevent accidental contact with biological samples.
„Top“: The packaged unit must always be transported and stored so that the arrows point upwards at any time.
"Protect from wetness": The packaged unit must be protected from excessive humidity and must be stored and transported in covered.
The Microtome M530 must be installed on a stable, level and horizontally oriented base to ensure the safe and reliable operation.
1.8. Protectiom measures of the device
1.9. Intended Use
The Microtome is a highly efficient multi-purpose microtome which can be used for any tasks
in paraffin, hard- and thin-sectioning techniques in biology, medicine, industry and research.
A use in food industries and similar facilities is strictly prohibited. Arbitrary rebuilding or
changing of this laboratory instrument is strictly prohibited due to safety reasons. Use only
original MEDITE spare parts in case of replacement issues.
Operation, maintenance or service conditions mentioned in this manual have to be strictly observed.
1.10. Approved Operators
Only persons who have been thoroughly trained by the operator and commissioned can handle the Microtome M530. The operator is responsible for other people in the work area. The operating company make the instruction manual accessible to the operator and make sure, that the operator have read and understand the instruction manual.
Unauthorized modifications to the device are forbidden due to safety reasons. When replacement damaged parts of the device only original spare parts must be used.
You should always activate these two safety precautions, when the
Microtome is not in use.
The handwheel brake – if activated – keeps the handwheel in its position
and protect the operator from crushing hazards.
Don’t use the device in an explosive area.
The knife carrier comes with a finger protection, which always should be
If you want to move the specimen away from the blade tip button (6) each tip moves the
specimen 6µm away from the blade. Hold pressing button (6) until second acustic signal
appears moves the specimen automatically into home position. You can stop that
automatically movement by again tip button (6).
Check if "Trim" is shown on the display. Set the slice thickness by turning the multiple
functional knob (Example 50 µm). The display shows the currently set cut thickness.
Cut the sample by turning the hand wheel until the desired object area is reached.
4.5.2. Sectioning the specimen
Check if "Fine" is shown on the display. Set the section thickness by turning the multiple
functional knob (Example 5 µm). The display shows the currently set cut thickness.
Cut the sample by turning the hand wheel until the desired object area is reached.
4.5.3. Automatic Retraction
To prevent the knife and the specimen from damage when the object clamp is moving up again, the specimen can be pulled back during upward movement by activating the automatic “Retraction”. The “Retraction” can be switched on and off by pressing button (2).
4.5.4. Position Memory
The M530 offers the possibility to store 3 values for trimming positions. These values can be used to recover certain positions within a short time. A position can be stored by pushing one of the button (3), (4) or (5) for more than one second until a signal appears. The value of the actual positioning then is stored in your chosen memory and can be approached any time later on by just pushing the memory key again. Occupied memory numbers are shown “M1 … M3” including their values in the display to avoid an overwriting of your positions. Of course you can overwrite your values just by proceding the same way again.
If the function is activated there is an “R” appearing in the display.
Press the multiple functional knob to switch between the trim and fine-cut
mode.
Press the multiple functional knob to switch between the trim and fine-cut