INHERITANCE OF COAT COLOUR AND TYPE OF FLEECE IN ALPACA RENIERI Carlo University of Camerino School of Environmental Sciences Animal Production Unit Italy
Jan 17, 2015
INHERITANCE OF COAT COLOUR AND TYPE OF
FLEECE IN ALPACA
RENIERI CarloUniversity of Camerino
School of Environmental SciencesAnimal Production Unit
Italy
RESEARCH GROUPProf. Carlo Renieri, Senior Researcher
Dr Marco Antonini, researcherProf. Alessandro Valbonesi, reseracher
Dr. Antonietta La Terza, PhD Tutor
Vincenzo La Manna, PhD tutor
Dr. Dario Pediconi, Post doc
Siva Arumugam Saravanaperumal, PhD candidateChandramohan Bathrachalam, PhD candidate
Gabriela Molina, PhD candidateAnnalisa Candelori, Phd candidate
Experimental Segregation DesignExperimental Segregation Design
An experimental trial involving 17 alpaca rams and 230 alpaca An experimental trial involving 17 alpaca rams and 230 alpaca dams was performed at the Experimental Station of dams was performed at the Experimental Station of Quimsachata, Peru. The Station is located on the Andean Quimsachata, Peru. The Station is located on the Andean Plateau at 4300 m under the management of the INIA ILLPA Plateau at 4300 m under the management of the INIA ILLPA PUNO. The trial is organised in a hierarchical scheme as PUNO. The trial is organised in a hierarchical scheme as follows:follows:
One hundred forty nine One hundred forty nine (149) crias were born. At (149) crias were born. At birth, the type of fleece birth, the type of fleece and the coat colour were and the coat colour were identified. Blood samples identified. Blood samples and skin biopsies for and skin biopsies for molecular genetic analysis molecular genetic analysis were sampled from were sampled from parents and crias.parents and crias.
CROSSCROSS RAMSRAMS DAMSDAMS
White x WhiteWhite x White2 Suri2 Suri 30 Huacaya30 Huacaya
2 Huacaya2 Huacaya 30 Suri30 Suri
White x White x ColouredColoured
2 Suri2 Suri 30 Huacaya Café30 Huacaya Café
2 Huacaya2 Huacaya10 Suri Lf + 8 Ap + 10 Suri Lf + 8 Ap + GrGr
Black x BlackBlack x Black2 Suri2 Suri 30 Huacaya30 Huacaya
2 Huacaya2 Huacaya 17 Suri17 Suri
Black x BrownBlack x Brown1 Suri1 Suri 15 Huacaya15 Huacaya
1 Huacaya1 Huacaya 15 Suri15 Suri
Brown x Brown x BrownBrown
2 Suri2 Suri 30 Huacaya30 Huacaya
1 Huacaya1 Huacaya 15 Suri15 Suri
TotalTotal 1717 230230
• RENIERI C:, VALBONESI A., LA MANNA V., ANTONINI M., ASPARRIN M., 2009. Inheritance of Suri and Huacaya type of fleece in alpaca. Italian J. Anim. Sci., 8, 83-91.
• VALBONESI A., PACHECO C., LEBBORONI G., ANTONINI M., RENIERI C., 2009. Phenotipic and genetic variation of fleece weight, fineness of fibre and its coefficient of variability in Peruvian alpaca. EAAP Annual Meeting 2009, abstract
• VALBONESI A., APAZA CASTILLO N., LA MANNA V., GONZALES CASTILLO M.L., HUANCA MAMANI T., RENIERI C., 2009. Inheritance of white, black and brown coat color in alpaca by segregation analysis. Eaap Annual Meeting 2009, abstract 3947
• CREPALDI P., MILANESI E., NICOLOSO L., LA MANNA V., RENIERI C., 2009. Evualuation of MC1R gene polymorphism in Vicugna pacos. EAAP Annual Meeting 2009, abstract 4093.
• BATHRACHALAM C., LA MANNA V., RENIERI C., LA TERZA A., 2009. Asip and MC1R in coat color variation in Alpaca. Eaap 2009, abstract 4398.
• PRESCIUTTINI S., VALBONESI A., APAZA N., ANTONINI M., HUANCA T., RENIERI C., 2010. Fleece variation in alpaca (Vicugna pacos): a two-locus model for the Suri/Huacaya phenotype. BMC Genetics 2010, 11:70. http://www.biomedcentral.com/1471-2156/11/70
• ALLAIN D., RENIERI C., 2010. Genetics of fibre production and fleece characteristics in small ruminants, Angora rabbit and South American camelids. Animal, 2010 (4) 9: 1472-1481
• La Manna V., La Terza A., Grezzi S., Saravanaperumal S.A., Apaza N., Huanca T., Renieri C., Bozzi R., 2010. A microsatellite study on the genetic distance between suri and huacaya sub populations in Peruvian Alpacas (Vicugna pacos). BMC Genetics, submitted.
• Chandramohan B., La Manna V., Renieri C., La Terza A., 2010. Asip and MC1R genes in Alpaca. WCGALP, PP2-154, p. 238
FULL WHITEDominante or recessive ?
• Dominante– Bustinza (1968)– Davis (2010)
• Recessive– Gandarillas (1971)
BUSTINZA (1968)
• WHITE x WHITE– 619 full and spotted white– 387 solid
• WHITE x SOLID– 746 full and spotted white– 712 solid
DAVID (2010)
• WHITE x WHITE– 71 full and spotted white– 30 solid
• WHITE x SOLID– 91 full and spotted white– 108 solid
BLACK vs BROWN
• DOMINANCE OF BLACK– Davis (2010)
• DOMINANCE OF BROWN– Velasco (1981)– Gandarillas (1971)
VELASCO (1981)
• BLACK x BLACK– 5 black
• BLACK x BROWN– 3 black– 5 brown
• BROWN x BROWN– 4 black– 27 brown
DAVIS (2010)
• BLACK x BLACK– 44 black– 7 brown
• BLACK x BROWN– 26 black– 17 brown
• BROWN x BROWN– 16 black– 69 brown
Conclusions for white (1)
• The inheritance of white can be defined by a single gene segregation, without any modifying effect.
• It is independent and completely dominant on pigmented animals, without any difference in segregation on black and brown pattern.
• This hypothesis is fully supported by the segregations observed in crosses involving white rams and pigmented dams , as well as in crosses of white parents, assuming that the frequency of heterozygous females ranges from 35% up to 100%.
Conclusion for white (2)
• White in mammals arise from improper melanoblast development or survival, reflecting absence of mature melanocytes.
• White can be caused by defects at various stages of melanocytes development, including proliferation, survival, migration, invasion of the integument, hair follicle entry and melanocytes stem cell renewal (Baxter et al., 2004).
• Many white traits have been identified in mouse and man, and 10 of the genes have been cloned (Baxter et al., 2004).
• The hypothesis is that the gene for white in alpaca is among these loci.
Conclusion for black and brown
• Black is dominant over brown.
• This hypothesis is fully supported by the segregations observed in crosses involving black rams and brown dams , as well as in crosses of black parents, assuming that the frequency of heterozygous females ranges from 54% up to 100%.
API Faint BrownLight Brown
Grey BlackBrown
Alpaca – Huacaya TypeInternational Year of
Natural Fibers 2009
Alpaca – Suri Type
APIBrown
Redish brownGreyWhite
Black
International Year of Natural Fibers
2009
Mechanisam of action of Asip and MC1R
White (BL) X Brown (CA)
S0502BL
282298CA
076108LF
EEI-024NE
297204CC
072108BL
Black (NE) X Brown (CC)
Pedigree chart of samples in progressPedigree chart of samples in progress
• Up to now we amplified full coding and 3’ UT region of Asip and MC1R
• the full coding region of Asip comprises of 402 bp and it codes for a protein of 133 aa and 3’ UTR comprises of 243 bp
• the entire coding region for MC1R comprises of 954 bp and it codes for a protein of 317 aa and 3’UTR includes 626 bp
• Structure of AsipAsip mRNA
Our findings
AAAAAAAAA
3’ Un Translated Region
ATG TGA
Coding Region5’ UTR ???
AAAAAAAAA
3’ Un Translated Region
ATG TGA
Coding Region5’ UTR ???
402 bp402 bpBp ???Bp ??? 243 bp243 bp
626 bp626 bp954 bp954 bpBp ???Bp ???
Structure of MC1RMC1R mRNA
MC1R
Asip
MC1RMC1R sequence alingment of Black X Cafe claro sequence alingment of Black X Cafe claro 10 20 30 40 50 60 70 80 90 .........|.........|.........|.........|.........|.........|.........|.........|.........|ATGCCTGTGCTCGGCCCCCAGAGGAGGCTGCTGGGCTCCCTCAACTCCACCCCCCAAGCCACCACCCACCTCGGACTGGCCACCAACCAG ATGCCTGTGCTCGGCCCCCAGAGGAGGCTGCTGGGCTCCCTCAACTCCACCCCCCAAGCCACCACCCACCTCGGACTGGCCACCAACCAG ATGCCTGTGCTCGGCCCCCAGAGGAGGCTGCTGGGCTCCCTCAACTCCACCCCCCAAGCCACCACCCACCTCGGACTGGCCGCCAACCAG 100 110 120 130 140 150 160 170 180 .........|.........|.........|.........|.........|.........|.........|.........|.........|ACGGGGCCCCAGTGCCTGGAGGTGTCTGTTCCCGATGGGCTGTTCCTCAGCCTGGGGCTGGTGAGCCTCGTGGAGAACGTGCTGGTGGTGACGGGGCCCCAGTGCCTGGAGGTGTCTGTTCCCGATGGGCTGTTCCTCAGCCTGGGGCTGGTGAGCCTCGTGGAGAACGTGCTGGTGGTGATGGGGCCCCAGTGCCTGGAGGTGTCTGTTCCCGACGGGCTGTTCCTCAGCCTGGGGCTGGTGAGCCTCGTGGAGAACGTGCTGGTGGTG 190 200 210 220 230 240 250 260 270 .........|.........|.........|.........|.........|.........|.........|.........|.........|GCTGCCATCACCAAGAACCGCAACCTGCATTCTCCCATGTATTACTTCATCTGCTGCCTGGCCGCGTCGGACCTGCTGATGAGCATGAGCGCTGCCATCACCAAGAACCGCAACCTGCATTCTCCCATGTATTACTTCATCTGCTGCCTGGCCGCGTCGGACCTGCTGATGAGCATGAGCGCTGCCATCACCAAGAACCGCAACCTGCATTCTCCCATGTATTACTTCATCTGCTGCCTGGCCGCGTCGGACCTGCTGGTGAGCATGAGC 280 290 300 310 320 330 340 350 360 .........|.........|.........|.........|.........|.........|.........|.........|.........|AACGTGCTGGAGACGGCCGTCATGCTGCTGCTGGAGGCTGGCGCCCTGGCCACATGGGCTACGGTGGTGCAGCAGCTGGACAATGTCATGAACGTGCTGGAGACGGCCGTCATGCTGCTGCTGGAGGCTGGCGCCCTGGCCACATGGGCTACGGTGGTGCAGCAGCTGGACAATGTCATGAACGTGCTGGAGACGGCCGTCATGCTGCTGCTGGAGGCTGGCGCCCTGGCCACATGGGCTACGGTGGTGCAGCAGCTGGACAAGGTCATG 370 380 390 400 410 420 430 440 450 .........|.........|.........|.........|.........|.........|.........|.........|.........|GATGTGCTCATCTGCAGCTCCATGGTGTCCAGCCTCTGCTCTCTGGGTGCTATCGCCGTGGACCGCTACATCTCCATCTTCTATGCACTGGATGTGCTCATCTGCAGCTCCATGGTGTCCAGCCTCTGCTCTCTGGGTGCTATCGCCGTGGACCGCTACATCTCCATCTTCTATGCACTGGATGTGCTCATCTGCGGCTCCATGGTGTCCAGCCTCTGCTCTCTGGGTGCTATCGCCGTGGACCGCTACATCTCCATCTTCTATGCACTG 460 470 480 490 500 510 520 530 540 .........|.........|.........|.........|.........|.........|.........|.........|.........|CGCTACCACAGCATCGTGACGCTGCCTCGGGCATGGCGGGCCATCGCGGCCATCTGGGTGGCCAGCGTCCTCTCCAGCACCCTCTTCATCCGCTACCACAGCATCGTGACGCTGCCTCGGGCATGGCGGGCCATCGCGGCCATCTGGGTGGCCAGCGTCCTCTCCAGCACCCTCTTCATCCGCTACCACAGCATCGTGACGCTGCCTCGGGCATGGCGGGCCATCGCGGCCATCTGGGTGGCCAGCGTCCTCTCCAGCACCCTCTTCATC 550 560 570 580 590 600 610 620 630 .........|.........|.........|.........|.........|.........|.........|.........|.........|ACCTACTATGATCACACAGCCGTCCTCCTCTGTCTCGTCAGCTTTTTTGTAGCCATGCTGGCGCTCATGGCGGTGCTGTATGTCCACATGACCTACTATGATCACACAGCCGTCCTCCTCTGTCTCGTCAGCTTTTTTGTAGCCATGCTGGCGCTCATGGCGGTGCTGTATGTCCACATGACCTACTATGATCACACAGCCGTCCTCCTCTGTCTCGTCAGCTTTTTTGTAGCCATGCTGGCGCTCATGGCGGTGCTATATGTCCACATG 640 650 660 670 680 690 700 710 720 .........|.........|.........|.........|.........|.........|.........|.........|.........|CTGGCCCGGGCGTGCCAGCATGCCCGGGGCATCGCCCAGCTCCACAAGAGACAGCGCCCCATCCACCAGGGCTTTGGCCTCAAGGGCGTGCTGGCCCGGGCGTGCCAGCATGCCCGGGGCATCGCCCAGCTCCACAAGAGACAGCGCCCCATCCACCAGGGCTTTGGCCTCAAGGGCGTGCTGGCCCGGGCGTGCCAGCATGCCCGGGGCATCGCCCAGCTCCACAAGAGACAGCGCCCCATCCACCAGGGCTTTGGCCTCAAGGGCGTG 730 740 750 760 770 780 790 800 810 .........|.........|.........|.........|.........|.........|.........|.........|.........|GCCACGCTCACCATCCTGCTGGGCATCTTCTTCCTCTGCTGGGGCCCCTTCTTCCTGCACCTTTTCCTCATCGTCCTCTGTCCTCAGCACGCCACGCTCACCATCCTGCTGGGCATCTTCTTCCTCTGCTGGGGCCCCTTCTTCCTGCACCTTTTCCTCATCGTCCTCTGTCCTCAGCACGCCACGCTCACCATCCTGCTGGGCATCTTCTTCCTCTGCTGGGGCCCCTTCTTCCTGCACCTTTTCCTCATCGTCCTCTGTCCTCAGCAC 820 830 840 850 860 870 880 890 900 .........|.........|.........|.........|.........|.........|.........|.........|.........|CTTTTCCTCATCGTCCTCTGTCCTCAGCACAACCTCTTCCTTGCCCTCATCATCTGCAACTCCATCGTGGACCCCCTCATCTATGCCTTCCTTTTCCTCATCGTCCTCTGTCCTCAGCACAACCTCTTCCTTGCCCTCATCATCTGCAACTCCATCGTGGACCCCCTCATCTATGCCTTCCTTTTCCTCATCGTCCTCTGTCCTCAGCACAACCTCTTCCTTGCCCTCATCATCTGCAACTCCATCGTGGACCCCCTCATCTATGCCTTC 910 920 930 940 950.........|.........|.........|.........|.........|.........|CGCAGCCAGGAGCTCCGGAAGACACTCCAGGAGGTGCTGCAGTGCTCCTGGTGATGCAGCCAGGAGCTCCGGAAGACACTCCAGGAGGTGCTGCAGTGCTCCTGGTGATGCAGCCAGGAGCTCCGGAAGACACTCCAGGAAGTGCTGCAGTGCTCCTGGTGA
Father (bLACK)Father (bLACK)Mother (Cafe claro)Mother (Cafe claro)F1 (white)F1 (white)
TTTTAA
RRCCCC
MMMMVV
SSSSGG
Father (bLACK)Father (bLACK)Mother (Cafe claro)Mother (Cafe claro)F1 (white)F1 (white)
Father (bLACK)Father (bLACK)Mother (Cafe claro)Mother (Cafe claro)F1 (white)F1 (white)
Father (bLACK)Father (bLACK)Mother (Cafe claro)Mother (Cafe claro)F1 (white)F1 (white)
Father (bLACK)Father (bLACK)Mother (Cafe claro)Mother (Cafe claro)F1 (white)F1 (white)
Father (bLACK)Father (bLACK)Mother (Cafe claro)Mother (Cafe claro)F1 (white)F1 (white)
Father (bLACK)Father (bLACK)Mother (Cafe claro)Mother (Cafe claro)F1 (white)F1 (white)
Father (bLACK)Father (bLACK)Mother (Cafe claro)Mother (Cafe claro)F1 (white)F1 (white)
Father (bLACK)Father (bLACK)Mother (Cafe claro)Mother (Cafe claro)F1 (white)F1 (white)
Father (bLACK)Father (bLACK)Mother (Cafe claro)Mother (Cafe claro)F1 (white)F1 (white)
Father (bLACK)Father (bLACK)Mother (Cafe claro)Mother (Cafe claro)F1 (white)F1 (white)
10 20 30 40 50 60 70 80 90 .........|.........|.........|.........|.........|.........|.........|.........|.........|ATGCCTGTGCTCGGCCCCCAGAGGAGGCTGCTGGGCTCCCTCAACTCCACCCCCCAAGCCACCACCCACCTCGGACTGGCCGCCAACCAG ATGCCTGTGCTCGGCCCCCAGAGGAGGCTGCTGGGCTCCCTCAACTCCACCCCCCAAGCCACCACCCACCTCGGACTGGCCACCAACCAG ATGCCTGTGCTCGGCCCCCAGAGGAGGCTGCTGGGCTCCCTCAACTCCACCCCCCAAGCCACCACCCACCTCGGACTGGCCACCAACCAG 100 110 120 130 140 150 160 170 180 .........|.........|.........|.........|.........|.........|.........|.........|.........|ACGGGGCCCCAGTGCCTGGAGGTGTCTGTTCCCGACGGGCTGTTCCTCAGCCTGGGGCTGGTGAGCCTCGTGGAGAACGTGCTGGTGGTGACGGGGCCCCAGTGCCTGGAGGTGTCTGTTCCCGATGGGCTGTTCCTCAGCCTGGGGCTGGTGAGCCTCGTGGAGAACGTGCTGGTGGTGATGGGGCCCCAGTGCCTGGAGGTGTCTGTTCCCGATGGGCTGTTCCTCAGCCTGGGGCTGGTGAGCCTCGTGGAGAACGTGCTGGTGGTG 190 200 210 220 230 240 250 260 270 .........|.........|.........|.........|.........|.........|.........|.........|.........|GCTGCCATCACCAAGAACCGCAACCTGCATTCTCCCATGTATTACTTCATCTGCTGCCTGGCCGCGTCGGACCTGCTGGTGAGCATGAGCGCTGCCATCACCAAGAACCGCAACCTGCATTCTCCCATGTATTACTTCATCTGCTGCCTGGCCGCGTCGGACCTGCTGATGAGCATGAGCGCTGCCATCACCAAGAACCGCAACCTGCATTCTCCCATGTATTACTTCATCTGCTGCCTGGCCGCGTCGGACCTGCTGGTGAGCATGAGC 280 290 300 310 320 330 340 350 360 .........|.........|.........|.........|.........|.........|.........|.........|.........|AACGTGCTGGAGACGGCCGTCATGCTGCTGCTGGAGGCTGGCGCCCTGGCCACATGGGCTACGGTGGTGCAGCAGCTGGACAACGTCATGAACGTGCTGGAGACGGCCGTCATGCTGCTGCTGGAGGCTGGCGCCCTGGCCACATGGGCTACGGTGGTGCAGCAGCTGGACAATGTCATGAACGTGCTGGAGACGGCCGTCATGCTGCTGCTGGAGGCTGGCGCCCTGGCCACATGGGCTACGGTGGTGCAGCAGCTGGACAATGTCATG 370 380 390 400 410 420 430 440 450 .........|.........|.........|.........|.........|.........|.........|.........|.........|GATGTGCTCATCTGCGGCTCCATGGTGTCCAGCCTCTGCTCTCTGGGTGCTATCGCCGTGGACCGCTACATCTCCATCTTCTATGCACTGGATGTGCTCATCTGCAGCTCCATGGTGTCCAGCCTCTGCTCTCTGGGTGCTATCGCCGTGGACCGCTACATCTCCATCTTCTATGCACTGGATGTGCTCATCTGCGGCTCCATGGTGTCCAGCCTCTGCTCTCTGGGTGCTATCGCCGTGGACCGCTACATCTCCATCTTCTATGCACTG 460 470 480 490 500 510 520 530 540 .........|.........|.........|.........|.........|.........|.........|.........|.........|CGCTACCACAGCATCGTGACGCTGCCTCGGGCATGGCGGGCCATCGCGGCCATCTGGGTGGCCAGCGTCCTCTCCAGCACCCTCTTCATCCGCTACCACAGCATCGTGACGCTGCCTCGGGCATGGCGGGCCATCGCGGCCATCTGGGTGGCCAGCGTCCTCTCCAGCACCCTCTTCATCCGCTACCACAGCATCGTGACGCTGCCTCGGGCATGGCGGGCCATCGCGGCCATCTGGGTGGCCAGCGTCCTCTCCAGCACCCTCTTCATC 550 560 570 580 590 600 610 620 630 .........|.........|.........|.........|.........|.........|.........|.........|.........|ACCTACTATGATCACACAGCCGTCCTCCTCTGTCTCGTCAGCTTTTTTGTAGCCATGCTGGCGCTCATGGCGGTGCTATATGTCCACATGACCTACTATGATCACACAGCCGTCCTCCTCTGTCTCGTCAGCTTTTTTGTAGCCATGCTGGCGCTCATGGCGGTGCTGTATGTCCACATGACCTACTATGATCACACAGCCGTCCTCCTCTGTCTCGTCAGCTTTTTTGTAGCCATGCTGGCGCTCATGGCGGTGCTGTATGTCCACATG 640 650 660 670 680 690 700 710 720 .........|.........|.........|.........|.........|.........|.........|.........|.........|CTGGCCCGGGCGTGCCAGCATGCCCGGGGCATCGCCCAGCTCCACAAGAGACAGCGCCCCATCCACCAGGGCTTTGGCCTCAAGGGCGTGCTGGCCCGGGCGTGCCAGCATGCCCGGGGCATCGCCCAGCTCCACAAGAGACAGCGCCCCATCCACCAGGGCTTTGGCCTCAAGGGCGTGCTGGCCCGGGCGTGCCAGCATGCCCGGGGCATCGCCCAGCTCCACAAGAGACAGCGCCCCATCCACCAGGGCTTTGGCCTCAAGGGCGTG 730 740 750 760 770 780 790 800 810 .........|.........|.........|.........|.........|.........|.........|.........|.........|GCCACGCTCACCATCCTGCTGGGCATCTTCTTCCTCTGCTGGGGCCCCTTCTTCCTGCACCTTTTCCTCATCGTCCTCTGTCCTCAGCACGCCACGCTCACCATCCTGCTGGGCATCTTCTTCCTCTGCTGGGGCCCCTTCTTCCTGCACCTTTTCCTCATCGTCCTCTGTCCTCAGCACGCCACGCTCACCATCCTGCTGGGCATCTTCTTCCTCTGCTGGGGCCCCTTCTTCCTGCACCTTTTCCTCATCGTCCTCTGTCCTCAGCAC 820 830 840 850 860 870 880 890 900 .........|.........|.........|.........|.........|.........|.........|.........|.........|CTTTTCCTCATCGTCCTCTGTCCTCAGCACAACCTCTTCCTTGCCCTCATCATCTGCAACTCCATCGTGGACCCCCTCATCTATGCCTTCCTTTTCCTCATCGTCCTCTGTCCTCAGCACAACCTCTTCCTTGCCCTCATCATCTGCAACTCCATCGTGGACCCCCTCATCTATGCCTTCCTTTTCCTCATCGTCCTCTGTCCTCAGCACAACCTCTTCCTTGCCCTCATCATCTGCAACTCCATCGTGGACCCCCTCATCTATGCCTTC 910 920 930 940 950.........|.........|.........|.........|.........|.........|TGCAGCCAGGAGCTCCGGAAGACACTCCAGGAAGTGCTGCAGTGCTCCTGGTGACGCAGCCAGGAGCTCCGGAAGACACTCCAGGAGGTGCTGCAGTGCTCCTGGTGACGCAGCCAGGAGCTCCGGAAGACACTCCAGGAGGTGCTGCAGTGCTCCTGGTGA
Father (White)Father (White)Mother (Cafe)Mother (Cafe)F1 (Light fawn)F1 (Light fawn)
Father (White)Father (White)Mother (Cafe)Mother (Cafe)F1 (Light fawn)F1 (Light fawn)
Father (White)Father (White)Mother (Cafe)Mother (Cafe)F1 (Light fawn)F1 (Light fawn)
Father (White)Father (White)Mother (Cafe)Mother (Cafe)F1 (Light fawn)F1 (Light fawn)
Father (White)Father (White)Mother (Cafe)Mother (Cafe)F1 (Light fawn)F1 (Light fawn)
Father (White)Father (White)Mother (Cafe)Mother (Cafe)F1 (Light fawn)F1 (Light fawn)
Father (White)Father (White)Mother (Cafe)Mother (Cafe)F1 (Light fawn)F1 (Light fawn)
Father (White)Father (White)Mother (Cafe)Mother (Cafe)F1 (Light fawn)F1 (Light fawn)
Father (White)Father (White)Mother (Cafe)Mother (Cafe)F1 (Light fawn)F1 (Light fawn)
Father (White)Father (White)Mother (Cafe)Mother (Cafe)F1 (Light fawn)F1 (Light fawn)
Father (White)Father (White)Mother (Cafe)Mother (Cafe)F1 (Light fawn)F1 (Light fawn)
MC1RMC1R Sequence alignment of White X Cafe Sequence alignment of White X Cafe
AATTTT
TTTTMM
CCRRRR
GGSSGG
Conclusion for Asip and MC1R
HIPÓTESIS GENÉTICAS
• Gene dominante: Velasco J., 1980
• Gene recesivo: Calle Escobar R., 1984
• Gene dominante o aplotipo: Ponzoni et al., 1997; Baychelier, 2000; Sponenberg, 2010).
VELASCO (1981)
• HUACAYA x HUACAYA– 129 H
• SURI x HUACAYA– 9 S– 3 H
• SURI x SURI– 422 S– 89 H
PONZONI et al. (1997)
• HUACAYA x HUACAYA– 145 H
• SURI x HUACAYA– 11 S– 13 H
• SURI x SURI– 29 S– 6 H
SPONENBERG (2010)
• HUACAYA x HUACAYA– 4 S– 19633 H
• SURI x HUACAYA– 56 S– 89 H
• SURI x SURI– 1702 S– 278 H
SURI FROM HUACAYA PARENTS
• Flint (1996)– 12 among 8,446 1.4
• Renieri et al. (2009)– 3 among 2,126 1.4
• Sponenberg (2010)– 4 among 19633 0.025
Nueva mutación dominante
Mutación con sobre exprésion genica
Tasa de mutación directa
= 3/2126 = 0.001411101 = 1,411101 x 10-3
CRUZAMIENTOS MACHOS HEMBRAS
BLANCO X BLANCO 2 SURI 30 HUACAYA
2 HUACAYA 30 SURI
BLANCO X COLOR 2 SURI 30 HUACAYA CAFÉ
2 HUACAYA 10 SURI LF + 8 AP + GR
COLOR X COLOR
NEGRO X NEGRO 2 SURI 30 HUACAYA
2 HUACAYA 17 SURI
NEGRO X CAFÉ 1 SURI 15 HUACAYA
1 HUACAYA 15 SURI
CAFÉ X CAFÉ 2 SURI 30 HUACAYA
1 HUACAYA 15 SURI
TOTAL : 17 230
HUACAYA GENOTYPE
DOBLE RECESSIVE
ab/ab
SURIGENOTYPE vs PHENOTYPE
NO SEGREGATING GENOTYPES
AA BBAA BbAa BBAa Bb
AA bbaa BB
SEGREGATINGGENOTYPES
Aa Bb
Aa bb
aa Bb
TWO LINKED LOCI
TEST CROSS
AB/ab x ab/ab
AB//abconfiguration CIS
Ab//aBconfiguracion TRANS
CONFIGURATION CISAB//ab x ab//ab
AB//ab SURI
ab//ab HUACAYA
Segregation ratio 1:1
THE MODEL IS INDISTINGUISHABLE FROM A
SINGLE LOCUS RECESSIVE MODEL
R1SEGREGATION RATIO
R1 = ½ - h Huacaya : ½ + h Suri
Ratio close to 1 : 1
CONFIGURATION TRANSAB//aB x ab//ab
Ab//ab SURI
aB//ab SURI
NO SEGREGATION OF HACAYA
RECOMBINATION h
R2SEGREGATION RATIO
R2 = ½ h Huacaya : 1 – ½ h Suri
Ratio close 0 : 1
hRECOMBINATION RATE
MAXIMUM LIKELIHOOD ESTIMATE
h =0,09995% confidence limits
0.029 – 0.204
Conclusions
• analysis of molecular variance (AMOVA), Nei’s and Cavalli-Sforza’s distance all suggest that there is no genetic differentiation between the two Suri and Huacaya populations for the studied loci.