Influence of 2D and 3D Environments on Osteogeneic Differentiation in hMSCs Jacqueline Mimnaugh, RET Neuqua Valley High School Dr. Richard Gemeinhart Melanie Kollmer UIC Department of Biopharmaceutical Sciences Tracy Chuong, REU University of California Berkley
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Influence of 2D and 3D Environments on Osteogeneic Differentiation in hMSCs Jacqueline Mimnaugh, RET Neuqua Valley High School Dr. Richard Gemeinhart Melanie.
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Influence of 2D and 3D Environments on Osteogeneic Differentiation in hMSCs
Jacqueline Mimnaugh, RET
Neuqua Valley High School
Dr. Richard Gemeinhart
Melanie Kollmer
UIC Department of Biopharmaceutical Sciences
Tracy Chuong, REU
University of California Berkley
Stem cells differentiate into other types of cells
Two major categories:
What are hMSCs?
Human Mesenchymal Stem Cells (hMSCs)
Isolated from marrow
Can differentiate into bone, cartilage, fat
Embryonic(Pluripotent)
Adult(Multipotent)
Induce hMSCs to develop into bone cells Osteogenesis
Tissue Engineering• Bone diseases/defects, trauma, cancer,
mal-union/non-union fractures
Why are hMSCs important?
How could we make new bone tissue for therapeutic uses?
Cells in the lab are typically cultured on plates
2 D
The Problem
Cells in vivo (in an organism)
3 D
It is possible that cells grown in a 3D scaffold would:1. Be more like cells in vivo
2. Would not reach confluence as quickly
3. Could be implanted directly
How will a 2D and 3D environment effect the osteogenic differentiation of hMSCs?