Industriell bruk og nytte av enzymer by Kjartan Sandnes, Dr. Daglig leder og forskningsleder
Jan 08, 2016
Industriell bruk og nytte av enzymer
by
Kjartan Sandnes, Dr.Daglig leder og forskningsleder
Biorefining salmon co-products
Sediment
Water soluble protein
Oil
Water and enzymes
Exact time and temp
Enzymatic hydrolysis
Some key figures
• Norwegian sea areas 7 times the size of land area
• All included, Norway is the 13th largest country in the world
• Second largest seafood export nation
• 53 bill. NOK • 33 mill seafood meals on
the table every day
White fish (cod, haddock, saithe) 325.000Herring 239.000Aquaculture (salmon, rainbow trout) 350.000Total co-products 914.000
«Industrial fish» (to the fish meal- and oil industry 2012) 202.000T O T A L (not for direct human consumption) 1.116.000
Norway 2012:«Non-edible fish raw materials»
NOTE! Seaweeds and shellfish not included
Source: fhf, Sildesalgslaget
Source: fhf, Sintef, Sildesalgslaget
Biomega AS –a blue biorefinery
• 2000: Marine Bioproducts AS established
• 2002: Production established with patented continuous enzymatic hydrolysis process. No chemical processing aids required
• 2007: New site acquired for new factory
• 2011: Six patent families filed so far• 2011: 30.000 tons raw materials
processed . Turnover 107 mill NOK• 2012: New factory with a capacity of
60.000 tons annually (130 mill NOK inv.) - opened May 2012
6
Constant efforts to improve quality (cooling systems) and logistics
Raw material transportation to Biomega – heads and backbones
Page 8
Raw material transportation to Biomega – viscera in bulk by truck
9
New processing plantproduction from May 2012
Overview of new plant
10
Biorefining - current and next generation products
BIOACTIVE PEPTIDES MODIFIED LIPIDS POWDERS MARINE MINERALS PEPTONES PHOSPHOSLIPIDS EXTRACTS GLUCOSAMINESFLAVOURS CHONDROITIN
BIOACTIVE PEPTIDES
N E W A N D I N N O V A T I V E P R O C E S S E Senzyme technology – extraction – separation - isolation
E X A M P L E S
Herring Hydrolysates
Salmon Hydrolysates
Test sample HH 1 HH 2MB
HR 1MB
HR 2MB
HR 3
Peptide size distribution (% of total detected)
> 30 000 daltons 0 0 2.5 0.2 0.0
30 000 – 20 000 daltons 1.3 0 0.7 0.2 0.0
20 000 – 10 000 daltons 1.2 0.2 12.6 7.1 4.6
10 000 – 5 000 daltons 12.4 5.2 17.8 15.5 10.0
5 000 - 1 000 daltons 14.6 10 13.3 9.7 10.2
1 000 - 100 daltons 53.1 66.1 38.3 40.5 33.9
< 100 daltons 17.4 18.4 14.7 26.7 41.3
Rough estimated average Mw 2100 1000 4900 2800 1900
How enzymes can be used
SEM
Typical growth medium
Nitrogen sourceAmmonium salts,Nitrates,Peptone (amino acids/peptides)
Carbon sourceGlucose,Glycerol
MicronutrientsPhosphates,Sulphates,Trace metals,Vitamins
BacteriaFungi Mammalian cellsYeasts
EnzymesAntibiotics Vaccines Other
SEM
Mask writer
Saccharomyces cerevisiae Growth Curves
0
1
2
3
4
5
6
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
Time (h)
OD
600
LACH
SOYP
OXYE
CASA
GELP
BDYE
MBPT
highest AN from supplier 1
highest AN from supplier 2
“good for yeasts” from supplier 2
Thank you!