Industrial Training ppt

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3rd Aug 2015

SONAL

(Plant Bio-Technology--Algae)

Presentation on

Industrial Training

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INTRODUCTION

Haematococcus Pluvialis It is a fresh water species of chlorophyta from family Haematococcaceae. It is usually found in temperate region. It is a green microalgae contain astaxanthin i.e. strong antioxidant.More than 40g of astaxanthin (Keto-carotenoid) can be obtained from 1kg of dry biomass.Astaxanthin produced from Haematococcus Pluvialis is a main source for human consumption.

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ASTAXANTHIN

• Red fat soluble pigment• Higher antioxidant properties.• Used in various drugs, lotions and creams• Protects brain from nervous damage• It has anti-cancer, anti diabetic, anti-inflammation activities

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RW1-170P-PHOTOBIOREACTOR

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INOCULATION OF MEDIUM AND DEVELOPMENT OF GROWTH CURVE

Icubated Under Outdoor Direct Sunlight DAY DATE CULTURE CONDITION O.D pH

0th 09-06-15 CONTROL 0.00 7.02TEST-1 0.00 7.24TEST-2 0.00 7.25



Incubated Under Double Tube LightDAY DATE CULTURE CONDITION O.D pH



14th 26-06-15 CONTROL 0.00 8.06

TEST-1 0.09 8.12

TEST-2 0.08 8.34

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MORPHOLOGICAL IDENTIFICATION OF HAEMATOCOCCUS PLUVIALIS

AT 10X AT 45X AT 100X

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STANDARDIZATION OF DAILY HARVESTED VOLUME FROM MASS CULTIVATED ALGAE

At 0th day After 19 days

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OPTIMIZATION OF ALGAE AS GROWTH AFFECTED BY NITROGEN SOURCE

DAY DATE CULTURECONDITION

O.D. pH

0th 15-06-15 CONTROL 0.00 7.58

FLASK-1(0.5g) 0.00 7.57

FLASK-2(0.5g) 0.00 7.53

FLASK-1(1g) 0.00 7.54

FLASK-2(1g) 0.00 7.55

FLASK-1(1.5g) 0.00 7.54

FLASK-2(1.5g) 0.00 7.54

15th 29-06-15 CONTROL 0.00 6.47TEST-1 (0.5g) 0.13 6.37TEST-2(0.5g) 0.10 6.03TEST-1(1g) 0.07 6.31TEST-2(1g) 0.08 6.02

TEST-1(1.5g) 0.07 6.21TEST2(1.5g) 0.06 6.27

AFTER 15 DAYS

FLASKS(0.5g, 1g)

FLASKS(1.5g)

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OPTIMIZATION OF ALGAE AS GROWTH AFFECTED BY VITAMINS


O.D. pH

1st 19-06-15 CONTROL 0.00 8.02

FLASK-1(0.5ml) 0.00 8.06

FLASK-2 (1ml) 0.00 8.03

FLASK-3(1.5ml) 0.00 8.05

11th 29-06-15 CONTROL 0.00 8.11(6.11)

FLASK-1(0.5ml) 0.07 8.79 (6.16)

FLASK-2 (1ml) 0.07 8.80(6.20)

FLASK-3(1.5ml) 0.05 8.17(6.22)

25th 13-07-15 CONTROL 0.00 8.50

FLASK-1(0.5ml) 0.20 9.70

FLASK-2 (1ml) 0.27 9.67

FLASK-3(1.5ml) 0.13 9.00

At 1st day

After 25 days

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OPTIMIZATION OF ALGAE AS GROWTH AFFECTED BY LIGHT


O.D. pH

0th 16-06-15 CONTROL 0.00 6.06

FLASK-1(D.T.L) 0.00 6.07

FLASK-2(S.T.L) 0.00 6.12

FLASK-3(I) 0.00 6.01

FLASK-4(O) 0.00 6.09

9th 25-06-15 CONTROL 0.00 8.03

FLASK-1(D.T.L) 0.07 8.98

FLASK-2(S.T.L) 0.03 8.43

FLASK-3(I) 0.07 8.93

FLASK-4(O) 0.05 9.12

20th 06-07-15 CONTROL 0.00 6.23

FLASK-1(D.T.L) 0.17 6.38

FLASK-2(S.T.L) 0.10 6.26

FLASK-3(I) 0.21 6.19

FLASK-4(O) 0.19 6.41

AFTER 20 DAYS

UNDER DOUBLE TUBE AND SINGLE TUBE LIGHT

UNDER INDOOR AND OUTDOOR IN DIRECT SUNLIGHT

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OPTIMIZATION OF ALGAE AS GROWTH AFFECTED BY pH


O.D. pH

0th 22-06-15 CONTROL 0.00 7.28

FLASK-1(pH 7) 0.00 7.34

FLASK-2(pH 8) 0.00 7.32

FLASK-3(pH 9) 0.00 7.34

9th 01-07-15 CONTROL 0.00 7.88

FLASK-1(pH 7) 0.07 7.95

FLASK-2(pH 8) 0.05 8.58

FLASK-3(pH 9) 0.04 8.23

21st 13-07-15 CONTROL 0.00 7.91

FLASK-1(pH 7) 0.19 7.95

FLASK-2(pH 8) 0.22 8.87

FLASK-3(pH 9) 0.14 8.89

AFTER 21 DAYS

GROWTH AFFECTED BY pH

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TRANSESTERIFICATION

SEPARATED BIODIESEL IN A FUNNEL SEPARATED GLYCEROL

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BEFORE PASSING CURRENT AFTER PASSING CURRENT

HARVESTING OF MICROALGAE WITH ELECTROFLOCULATION

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B STRAIN OF ASTAXANTHIN (AHB)

AT 10X AT 45X AT 100X

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ISOLATION OF ALGAL CULTURE BY SKINNER’S TECHNIQUE

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ISOLATION OF ALGAL CULTURE BY SERIAL PLATE METHOD

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MARKET SCOPE OF ASTAXANTHIN

Market Sectors Market Size (as of 2009) ( Million USD)

Potential Market (2020) (Million USD)

Animal feed coloring agents

300 800

Antioxidant neutraceuticals

30 300

Pharmaceuticals Emerging 500

Cosmetics Emerging 30

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Industrial Training ppt

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